Standard name
Description Protein of unknown function; member of a flavodoxin-like fold protein family that includes Pst2p and Ycp4p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; RFS1 has a paralog, PST2, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.24 0.14 0.13 0.09 0.17 0.34 0.33 0.32 0.37 0.42 0.35 0.09 0.11 0.05 0.12 0.07 0 0.08 0.06 0 0.06 0 0
Bud 0 0 0 0 0 0.09 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.76 0.82 0.85 0.78 0.75 0.49 0.69 0.51 0.63 0.63 0.61 0.93 0.89 0.93 0.89 0.89 0.87 0.8 0.77 0.91 0.83 0.88 0.9
Cytoplasm 0.26 0.35 0.15 0.43 0.18 0.27 0.16 0.18 0.2 0.19 0.19 0.21 0.15 0.08 0.09 0.15 0.11 0 0.1 0 0 0 0
Endoplasmic Reticulum 0.11 0 0 0.05 0.09 0 0.1 0 0.07 0.07 0.06 0.07 0.05 0 0.06 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0.07 0 0 0 0.07 0 0 0 0 0.05 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.33 0 0.33 0.2 0.32 0.27 0 0 0 0.12 0.11 0.16 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 59 22 11 14 28 40 100 24 77 113 68 24 46 23 12 9 6 22 11 1 10 16 14
Bud 9 7 2 1 5 10 6 2 6 15 8 4 8 6 1 0 2 0 0 0 1 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 4 2 0 2 2
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 187 131 72 118 123 57 208 39 132 170 120 236 372 411 91 110 118 219 139 99 158 373 340
Cytoplasm 63 56 13 65 30 31 47 14 42 52 37 54 61 36 9 18 15 11 18 4 4 10 2
Endoplasmic Reticulum 28 2 3 8 14 4 30 3 15 18 11 19 20 9 6 1 5 12 2 0 3 10 6
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 2 2
Golgi 0 0 0 0 2 1 22 0 3 4 14 1 1 0 1 6 1 1 0 0 0 0 0
Mitochondria 1 1 0 0 0 38 11 25 41 86 52 0 0 1 12 14 22 1 0 0 7 2 1
Nucleus 0 0 0 0 0 2 2 3 1 4 1 0 0 0 1 0 0 0 0 0 0 0 0
Nuclear Periphery 1 1 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 2 1 0 0 1 1 0 1 0 1 0 1 0 0 0 0 0 0
SpindlePole 0 1 2 0 0 2 2 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 1 0 1 7 0 1 1 7 0 0 0 2 2 0 0 1 0 1 0 0
Unique Cell Count 246 160 85 151 163 116 302 76 208 268 196 254 419 441 102 124 135 276 181 109 192 423 376
Labelled Cell Count 348 221 103 207 202 188 436 110 318 465 321 338 509 487 136 160 170 276 181 109 192 423 376


Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 14.0 13.7 14.5 13.7 16.2 10.9 15.1 12.0 13.4 12.8 13.9 18.7 21.1 21.5 22.1 23.4 22.6 13.4 14.9 16.0
Std Deviation (1e-4) 2.2 2.2 2.0 2.2 2.8 2.6 2.4 2.8 3.0 2.7 4.0 2.9 3.7 4.2 4.8 9.6 4.8 3.0 2.8 3.1
Intensity Change (Log2) -0.08 0.16 -0.41 0.06 -0.27 -0.11 -0.18 -0.06 0.36 0.54 0.56 0.61 0.69 0.64 -0.12 0.04 0.14


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -0.9 0.9 3.5 3.6 2.9 4.1 4.9 3.7 -0.9 -0.5 -2.7 -0.2 -1.4 -2.3
Bud 0 0 1.9 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -1.2 -1.7 -5.2 -2.9 -4.6 -3.6 -3.7 -3.9 2.3 1.1 2.6 0.9 0.8 0.6
Cytoplasm 4.4 0.6 1.9 0.1 0.5 1.0 0.9 0.7 1.2 -0.2 -2.1 -1.4 -0.2 -0.9
Endoplasmic Reticulum 0 1.5 0 1.9 0 1.2 1.1 0 1.3 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 5.9 0 5.8 4.4 6.0 5.3 0 0 0 3.3 3.2 3.9
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rfs1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available