Standard name
Human Ortholog
Description Protein with a putative role in response to acid stress; null mutant is sensitive to acetic acid; transcription is regulated by Haa1p and induced in the presence of acetic acid; protein observed in plasma membrane foci in the presence of acetic acid; the authentic, non-tagged protein is detected in a phosphorylated state in highly purified mitochondria in high-throughput studies

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.07 0.05 0 0 0 0 0.09 0.05 0.13 0.18 0.22 0.2 0 0 0 0 0 0 0.08 0.05 0.11 0.07 0.09 0.06
Bud 0 0 0.06 0 0 0.07 0.08 0.08 0.08 0.13 0.15 0.12 0 0 0 0 0 0 0.07 0.06 0.05 0.07 0.07 0.09
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.08 0.07
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.08 0.09 0.09 0 0 0.08 0.16 0.13 0.26 0.31 0.38 0.38 0.05 0.07 0.1 0.05 0 0 0.18 0.15 0.22 0.15 0.1 0.15
Cytoplasm 0.83 0.87 0.87 0.92 0.9 0.78 0.56 0.45 0.45 0.33 0.33 0.3 0.96 0.93 0.92 0.88 0.88 0.88 0.4 0.57 0.42 0.39 0.31 0.32
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.06 0.08 0.05 0 0 0 0.06 0.05
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0 0 0.05 0.09 0.25 0.31 0.32 0.28 0.29 0.32 0.36 0 0 0 0.08 0 0 0.09 0.06 0.05 0.12 0.12 0.1
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0.06 0.09 0.07 0.1 0.06 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.13 0.08 0.11 0.06 0.08 0 0 0 0 0 0 0 0 0 0.09 0.1 0.09
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 9 10 8 1 9 20 77 46 71 115 131 143 3 7 16 2 2 3 11 10 25 15 25 20
Bud 5 7 13 7 21 41 73 71 42 84 88 84 1 4 1 1 1 1 9 13 11 15 22 29
Bud Neck 2 0 5 1 7 8 17 4 1 4 4 3 0 1 4 0 1 0 5 8 6 13 25 20
Bud Site 2 2 1 1 1 9 7 5 7 9 13 7 0 1 0 0 0 0
Cell Periphery 10 20 21 9 17 50 141 112 136 195 221 274 11 29 51 5 4 4 26 34 49 32 28 47
Cytoplasm 101 192 197 190 445 488 480 377 238 207 191 216 197 410 472 84 126 101 57 127 96 84 93 99
Endoplasmic Reticulum 2 1 0 0 0 1 6 3 1 4 6 4 0 2 2 7 8 9 6 2 5 5 18 15
Endosome 0 0 0 0 0 0 0 3 4 2 1 1 0 0 2 2 3 0 2 1 2 0 3 2
Golgi 0 0 0 1 0 0 0 0 5 0 0 0 1 0 0 0 1 3 0 0 0 0 1 1
Mitochondria 6 4 8 11 45 153 271 267 148 187 187 260 0 2 1 8 6 4 13 14 11 25 35 30
Nucleus 0 0 0 0 1 4 6 17 7 8 5 8 0 1 0 0 0 1 0 0 0 0 1 1
Nuclear Periphery 0 0 1 0 2 10 51 76 37 64 37 47 0 1 0 0 0 0 0 0 0 1 1 0
Nucleolus 0 0 0 1 1 1 0 12 5 3 7 8 0 0 0 0 1 0 0 0 0 0 0 0
Peroxisomes 0 0 0 2 0 6 19 11 5 4 5 3 0 0 1 0 1 0 0 0 0 0 1 0
SpindlePole 0 1 0 2 1 6 34 25 17 20 23 21 1 5 6 2 3 1 1 1 4 1 3 6
Vac/Vac Membrane 0 0 1 1 2 5 32 113 42 70 34 56 0 2 4 1 1 1 4 6 6 19 28 29
Unique Cell Count 121 221 227 206 492 624 864 842 527 634 585 726 206 442 512 96 143 115 142 224 228 217 297 311
Labelled Cell Count 137 237 255 227 552 802 1214 1142 766 976 953 1135 214 465 560 112 158 128 142 224 228 217 297 311


Bud, Cell Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.0 4.7 3.4 3.4 3.2 3.6 4.2 6.2 7.8 8.8 10.7 12.4 5.3 5.1 5.4 5.0 5.0 5.0 8.3 8.1 7.7
Std Deviation (1e-4) 0.9 2.4 1.2 1.7 1.3 1.9 2.9 5.1 6.6 7.6 10.9 13.3 1.8 1.7 2.0 1.7 1.6 2.0 12.5 15.4 12.1
Intensity Change (Log2) -0.01 -0.09 0.08 0.29 0.84 1.17 1.35 1.63 1.84 0.61 0.57 0.64 0.54 0.53 0.54 1.27 1.23 1.17


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.5469 2.6842 1.2341 1.3825 0.1328 1.5185 0.684 5.2806 1.5747 1.5753 1.1208 2.2531 -0.5268 1.6659 0.3455 1.6887 1.7541 0.7289
Actin 0.0355 0.0114 0.0101 0.0086 0.003 0.0131 0.023 0.0014 0.012 0.001 0.0078 0.0154 0.0238 0.0024 0.0029 0.0035 0.0025 0.0073
Bud 0.0104 0.0242 0.0128 0.0111 0.0027 0.0141 0.0044 0.0037 0.0052 0.0031 0.0088 0.0036 0.0072 0.0091 0.014 0.0038 0.012 0.0075
Bud Neck 0.0081 0.0033 0.0059 0.006 0.0128 0.0229 0.0022 0.0012 0.0023 0.016 0.0035 0.0124 0.0026 0.0025 0.002 0.059 0.0014 0.0293
Bud Periphery 0.0383 0.0431 0.0503 0.1089 0.0068 0.0941 0.041 0.0137 0.0381 0.0178 0.0879 0.0563 0.0481 0.036 0.0508 0.0317 0.08 0.0793
Bud Site 0.0441 0.1163 0.0605 0.0324 0.0044 0.0073 0.0296 0.0663 0.0319 0.0057 0.002 0.0024 0.032 0.116 0.0335 0.0078 0.0033 0.0072
Cell Periphery 0.089 0.1935 0.1124 0.1506 0.0625 0.1344 0.0626 0.1449 0.1149 0.1054 0.1328 0.1724 0.2167 0.2194 0.1927 0.2853 0.3347 0.2853
Cytoplasm 0.1301 0.2005 0.125 0.1168 0.141 0.1676 0.1243 0.2302 0.1585 0.2294 0.1174 0.2324 0.1438 0.2563 0.24 0.2188 0.1182 0.2209
Cytoplasmic Foci 0.0357 0.0146 0.0192 0.0116 0.0448 0.021 0.0203 0.0062 0.0254 0.0058 0.008 0.0098 0.0142 0.006 0.0079 0.0139 0.0016 0.0099
Eisosomes 0.0041 0.0014 0.0041 0.0015 0.0006 0.001 0.0024 0.0015 0.0018 0.0002 0.0006 0.0006 0.0082 0.0021 0.0013 0.0016 0.0072 0.0011
Endoplasmic Reticulum 0.0057 0.0088 0.0061 0.0088 0.005 0.004 0.0052 0.0026 0.0113 0.0032 0.0011 0.003 0.0051 0.0083 0.0053 0.0045 0.0082 0.0038
Endosome 0.0213 0.0118 0.0242 0.0104 0.0465 0.0073 0.018 0.0051 0.0282 0.0064 0.0028 0.0198 0.0091 0.0128 0.0069 0.0028 0.0013 0.0047
Golgi 0.0047 0.0012 0.0021 0.0019 0.0075 0.0012 0.0076 0.0003 0.0059 0.0002 0.0006 0.0031 0.0024 0.0003 0.0005 0.0008 0.0002 0.0006
Lipid Particles 0.0111 0.0025 0.0056 0.0059 0.0199 0.0047 0.0231 0.0005 0.0074 0.0009 0.0002 0.002 0.004 0.002 0.003 0.0007 0.0019 0.0021
Mitochondria 0.0115 0.002 0.0089 0.0049 0.0055 0.0038 0.0173 0.0003 0.0154 0.0002 0.0086 0.0046 0.0021 0.0006 0.001 0.0007 0.0008 0.0016
None 0.4689 0.3184 0.4877 0.4575 0.5727 0.4407 0.5761 0.5142 0.5057 0.4906 0.5684 0.4118 0.4637 0.3011 0.4212 0.3517 0.3603 0.3207
Nuclear Periphery 0.0032 0.0022 0.0053 0.002 0.0022 0.0012 0.002 0.0003 0.0021 0.0007 0.0002 0.0004 0.0016 0.0009 0.0008 0.0006 0.0012 0.0006
Nucleolus 0.0034 0.0016 0.0041 0.0033 0.0038 0.0025 0.0011 0.0003 0.002 0.0003 0.0004 0.0004 0.0007 0.0004 0.0006 0.0004 0.0003 0.0007
Nucleus 0.0048 0.0049 0.0055 0.0107 0.0057 0.0054 0.0025 0.0008 0.0031 0.0015 0.0005 0.0008 0.001 0.0008 0.0016 0.0019 0.0012 0.0015
Peroxisomes 0.017 0.0007 0.0055 0.0024 0.0206 0.0036 0.0082 0.0001 0.0053 0.0001 0.0008 0.0003 0.0015 0.0001 0.0002 0.0005 0.0001 0.0004
Punctate Nuclear 0.0054 0.0061 0.0065 0.0104 0.0113 0.0109 0.0011 0.0002 0.001 0.0003 0.0001 0.0003 0.001 0.0003 0.0009 0.0006 0.0001 0.0013
Vacuole 0.0449 0.0305 0.0358 0.0327 0.0197 0.0377 0.0262 0.0061 0.0206 0.1066 0.0463 0.0461 0.0105 0.0223 0.0124 0.0093 0.0619 0.0136
Vacuole Periphery 0.0029 0.0009 0.0022 0.0016 0.0011 0.0015 0.0018 0.0002 0.0019 0.0044 0.001 0.0023 0.0005 0.0002 0.0003 0.0004 0.0015 0.0006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 4.2644 0.953 22.6298 27.2012 8.9845 5.3275 6.2634 10.0806 26.989 10.0888
Translational Efficiency

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1593 1655 232 387 1858 1582 2727 117 3451 3237 2959 504

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 646.20 724.20 4012.32 3129.34 663.48 688.19 1490.64 2590.98 655.50 706.60 1688.35 3004.36
Standard Deviation 79.96 110.42 1056.75 1114.88 79.12 96.69 585.14 902.63 79.97 105.48 928.75 1093.25
Intensity Change Log 2 0.164407 2.634384 2.275806 0.052754 1.167807 1.965373 0.108924 2.070993 2.126882

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.003460 0.005079 0.001602 0.002847 0.000434 0.005750 0.004707 0.002425 0.001831 0.005407 0.004463 0.002749
Bud Neck 0.051338 0.043780 0.006267 0.001164 0.025868 0.061734 0.008854 0.001964 0.037625 0.052554 0.008651 0.001350
Bud Site 0.049023 0.062733 0.010142 0.035712 0.009311 0.045411 0.137415* 0.028619 0.027642 0.054267 0.127436* 0.034066
Cell Periphery 0.006606 0.012773 0.919240* 0.903308* 0.002049 0.012232 0.817020* 0.948144* 0.004152 0.012509 0.825035* 0.913716*
Cytoplasm 0.437905* 0.396415* 0.000077 0.026687 0.451045* 0.390005* 0.019667 0.006266 0.444980* 0.393283* 0.018131 0.021947
Cytoplasmic Foci 0.207298* 0.232250* 0.000208 0.001812 0.244382* 0.251341* 0.000613 0.000070 0.227264* 0.241580* 0.000581 0.001407
Eisosomes 0.003573 0.002615 0.036414 0.001890 0.000635 0.005770 0.002512 0.005876 0.001991 0.004157 0.005170 0.002815
Endoplasmic Reticulum 0.001590 0.001906 0.001451 0.001677 0.000803 0.001008 0.001357 0.000280 0.001166 0.001467 0.001364 0.001353
Endosome 0.011940 0.016030 0.000380 0.000587 0.010974 0.019790 0.000424 0.000108 0.011420 0.017868 0.000421 0.000476
Golgi 0.004383 0.007416 0.001278 0.004311 0.002815 0.012043 0.000313 0.000172 0.003539 0.009677 0.000388 0.003350
Lipid Particles 0.015999 0.009187 0.000291 0.001133 0.010970 0.011540 0.000361 0.000005 0.013292 0.010337 0.000356 0.000871
Mitochondria 0.008346 0.010715 0.002239 0.002217 0.005006 0.017772 0.001707 0.002063 0.006548 0.014164 0.001749 0.002181
Mitotic Spindle 0.002034 0.002683 0.001075 0.002130 0.000813 0.001487 0.000288 0.000230 0.001377 0.002099 0.000350 0.001689
None 0.003849 0.002357 0.000086 0.000688 0.001489 0.001526 0.000072 0.000026 0.002578 0.001951 0.000073 0.000534
Nuclear Periphery 0.000300 0.000306 0.000907 0.000474 0.000423 0.000193 0.000066 0.000188 0.000366 0.000251 0.000132 0.000408
Nuclear Periphery Foci 0.000256 0.000312 0.000090 0.000850 0.000167 0.000231 0.000048 0.000022 0.000208 0.000272 0.000052 0.000658
Nucleolus 0.000898 0.001132 0.000174 0.000086 0.000858 0.000741 0.000026 0.000044 0.000876 0.000941 0.000038 0.000076
Nucleus 0.130964* 0.122144 0.000400 0.000698 0.181066* 0.090062 0.001215 0.000119 0.157939* 0.106465 0.001151 0.000563
Peroxisomes 0.010270 0.015287 0.000006 0.000050 0.007826 0.029383 0.000061 0.000008 0.008954 0.022176 0.000057 0.000041
Vacuole 0.049099 0.053075 0.014037 0.005614 0.042305 0.040460 0.002684 0.002310 0.045441 0.046910 0.003574 0.004847
Vacuole Periphery 0.000870 0.001805 0.003637 0.006064 0.000760 0.001517 0.000589 0.001060 0.000811 0.001664 0.000828 0.004902

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.53 2.17 0.50 1.90 -1.31 -5.96 -11.18 -1.13 1.66 1.29 -5.33 -5.53 -1.11 2.68 2.04
Bud Neck 2.04 13.63 16.69 18.54 3.70 -9.94 9.14 12.84 18.60 7.44 -5.75 16.13 21.37 26.27 11.40
Bud Site -2.31 8.22 1.93 3.77 -4.18 -10.16 -26.00* -1.96 1.58 10.04 -7.91 -20.79 -1.29 3.55 14.40
Cell Periphery -2.65 -101.64* -82.91* -81.30* 1.15 -5.07 -139.81* -63.43* -62.38* -8.31 -5.45 -151.24 -100.86 -99.08* -8.59
Cytoplasm 4.69 71.59* 49.89 43.76 -4.83 6.75 69.90* 56.68 45.15 2.61 8.17 94.48* 68.78 57.81 -0.73
Cytoplasmic Foci -3.34 40.68 39.24 41.93 -1.30 -0.93 48.11 48.25 43.22 5.09 -2.67 62.71 60.49 59.15 -0.80
Eisosomes 1.54 -5.78 2.54 1.07 6.07 -6.47 -9.58 -2.49 -0.08 -1.60 -4.32 -5.90 -1.29 1.72 3.00
Endoplasmic Reticulum -0.77 0.23 -0.15 0.35 -0.30 -1.06 -2.39 2.95 5.41 5.79 -1.34 -0.89 -0.41 0.23 0.03
Endosome -2.79 14.70 13.78 12.45 -0.82 -6.29 15.51 16.25 16.06 2.45 -6.39 21.01 20.04 19.66 -0.25
Golgi -3.67 6.47 0.02 1.57 -1.71 -8.59 8.30 9.28 11.44 1.19 -9.14 12.36 0.11 4.17 -2.19
Lipid Particles 5.31 13.24 11.36 10.55 -1.43 -0.64 16.77 17.93 14.77 2.28 3.70 19.77 16.06 14.88 -1.10
Mitochondria -1.52 4.40 4.35 6.87 0.02 -7.54 4.92 2.01 7.55 -0.26 -6.77 6.82 4.78 10.55 -0.60
Mitotic Spindle -0.39 1.03 -0.02 0.28 -0.70 -1.24 1.03 1.18 2.91 0.70 -1.00 1.96 -0.23 0.36 -1.15
None 1.74 4.63 3.61 4.13 -1.86 -0.30 12.45 12.88 7.93 5.45 1.47 6.57 4.49 4.87 -1.85
Nuclear Periphery -0.09 -6.58 -1.49 -1.33 3.02 5.96 12.01 3.39 0.13 -1.93 3.01 10.42 -0.45 -1.66 -3.13
Nuclear Periphery Foci -1.32 4.96 -0.86 -0.78 -1.11 -1.74 6.91 8.38 5.26 3.44 -2.36 8.86 -0.85 -0.72 -1.15
Nucleolus -1.22 7.45 8.43 5.49 4.41 0.34 6.38 6.24 6.96 -2.21 -0.79 10.12 9.63 8.00 -4.85
Nucleus 1.50 35.74 35.65 31.77 -2.62 16.77 42.59 42.97* 26.64 4.28 13.20 54.62 55.07 41.15 2.56
Peroxisomes -3.68 11.02 10.97 15.43 -2.99 -10.85 13.36 13.45 15.48 4.63 -11.10 16.72 16.75 20.82 1.08
Vacuole -1.59 14.78 20.89 21.35 4.71 0.81 24.41 22.36 20.32 0.61 -0.90 33.40 28.40 27.59 -1.34
Vacuole Periphery -4.65 -5.23 -4.25 -3.46 -1.83 -4.44 1.60 -1.41 1.73 -2.09 -6.46 -0.20 -4.34 -3.42 -4.30