Standard name
Human Ortholog
Description Guanine nucleotide exchange factor (GEF) for Cdc42p; required for polarity establishment and maintenance, and mutants have morphological defects in bud formation and shmooing; relocalizes from nucleus to cytoplasm upon DNA replication stress; thermosensitivity of the cdc24-4 mutant in the presence of sorbitol is functionally complemented by human CDC42
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.08 0.06 0.05 0.09 0.12 0.07 0 0.05 0 0 0 0.05 0.14 0.12 0.08 0.15 0.13 0.16 0.08 0.05 0.05 0 0 0
Bud 0.07 0.07 0.07 0.05 0.08 0.07 0.07 0.05 0.07 0 0.08 0.09 0.14 0.07 0.06 0.07 0.05 0.09 0.09 0.14 0.14 0.17 0.15 0.14
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0.05 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0.13 0.17 0.06 0.05 0.06 0 0 0 0 0 0
Cytoplasm 0.33 0.33 0.4 0.42 0.56 0.49 0.53 0.5 0.46 0.46 0.42 0.46 0.37 0.55 0.67 0.48 0.49 0.55 0.19 0.2 0.23 0.06 0 0
Endoplasmic Reticulum 0 0 0.06 0.05 0.05 0.05 0 0 0 0 0 0 0.12 0.1 0.11 0.06 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.06 0 0 0 0.08 0.15 0.06 0.33 0.21 0.35 0.19 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.41 0.31 0.34 0.32 0.17 0.19 0.17 0.14 0.18 0.21 0.17 0.13 0.22 0.13 0.05 0.21 0.15 0.13 0.27 0.23 0.23 0.24 0.23 0.24
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.1 0.24 0.15 0.14 0.11 0.13 0.11 0.2 0.09 0.16 0.12 0.16 0.07 0.08 0.05 0.13 0.19 0.14 0.21 0.27 0.2 0.32 0.39 0.41
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 15 13 11 24 35 24 22 28 17 4 7 17 20 29 21 44 20 18 17 14 11 11 13 5
Bud 13 15 15 13 22 22 33 28 30 15 28 32 21 17 16 19 7 10 20 35 34 45 59 62
Bud Neck 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0 8 13 14 13 17 9
Bud Site 0 1 1 1 1 2 3 8 10 1 12 6 2 0 0 2 1 0 – – – – – –
Cell Periphery 2 2 9 4 10 6 9 4 8 4 9 12 5 32 44 17 7 7 0 1 1 0 0 0
Cytoplasm 60 66 84 110 162 160 262 262 183 159 141 164 55 133 174 138 75 62 43 52 55 16 8 10
Endoplasmic Reticulum 6 3 13 12 13 17 21 23 11 6 6 8 18 23 29 17 6 2 4 1 4 10 15 14
Endosome 1 1 0 0 2 11 6 5 3 4 6 6 3 2 2 3 1 0 5 4 5 6 5 10
Golgi 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0 3 2 3 3 0 0 0 0 3
Mitochondria 6 13 2 7 4 27 73 29 132 72 118 70 6 1 1 2 3 5 1 2 8 7 10 14
Nucleus 75 62 70 82 49 62 85 73 72 74 58 46 32 32 13 61 23 15 61 62 55 64 89 105
Nuclear Periphery 0 0 0 0 0 0 0 3 0 2 0 0 0 0 1 0 0 0 1 0 0 2 8 9
Nucleolus 0 1 0 0 0 1 0 3 2 0 2 2 0 0 0 0 2 0 0 0 0 0 2 3
Peroxisomes 0 0 0 0 1 1 1 2 0 0 1 0 1 1 1 1 0 1 0 0 0 0 0 1
SpindlePole 0 1 1 2 6 1 4 12 3 0 2 13 1 0 0 5 2 4 1 2 1 2 2 7
Vac/Vac Membrane 18 49 32 37 32 44 53 106 36 54 41 57 10 20 12 38 30 16 47 71 49 87 153 181
Unique Cell Count 182 201 208 260 288 327 499 520 400 347 336 359 148 242 260 288 154 113 223 264 247 274 393 447
Labelled Cell Count 197 227 238 292 337 378 572 588 507 395 431 433 174 290 314 350 180 143 223 264 247 274 393 447


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.9 7.7 7.8 7.3 7.8 6.4 6.3 6.6 5.5 5.5 5.2 6.0 8.4 8.5 8.4 10.1 10.0 9.5 8.2 8.2 8.8
Std Deviation (1e-4) 1.5 1.3 1.1 1.1 1.3 1.1 1.0 1.3 1.3 1.1 1.0 1.1 1.4 1.2 1.3 1.6 1.7 1.6 1.1 1.2 1.6
Intensity Change (Log2) – – – -0.09 0.0 -0.3 -0.3 -0.23 -0.49 -0.5 -0.58 -0.38 0.11 0.13 0.11 0.37 0.36 0.29 0.07 0.08 0.17

WT3RAP60RAP140RAP220RAP300RAP380RAP460RAP540RAP620RAP7000510WT3HU80HU120HU1600510WT3rpd3Δ_1rpd3Δ_2rpd3Δ_30510WT1AF100AF140AF1800510
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 1.6 2.6 0.9 -0.5 0.1 -0.6 -2.9 -2.0 -0.3 2.7 2.5 1.2 3.5 2.6 3.2
Bud -1.0 0.2 -0.2 -0.3 -0.9 0.1 -1.5 0.5 0.7 2.2 -0.1 -0.5 -0.3 -1.0 0.5
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -1.8 -0.5 -1.7 -1.9 0 -1.7 0 -1.0 -0.6 -0.5 3.3 4.3 0.8 0.1 0.7
Cytoplasm 0.4 3.5 1.9 2.9 2.4 1.3 1.2 0.4 1.2 -0.6 3.1 5.7 1.7 1.6 2.5
Endoplasmic Reticulum -0.8 -0.9 -0.5 -1.2 -1.0 -2.1 -2.8 -2.8 -2.4 1.9 1.3 1.8 -0.2 -1.0 -1.8
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 3.6 5.4 2.8 9.0 6.6 9.3 6.4 0 0 0 0 0 0
Nucleus -0.5 -4.3 -3.8 -4.9 -6.0 -4.3 -3.2 -4.4 -5.9 -2.5 -5.2 -8.1 -3.1 -4.0 -4.0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 2.3 0 0 0 0 0 0
Vacuole -0.3 -1.4 -0.6 -1.8 1.6 -2.4 0.1 -1.1 0.2 -2.5 -2.4 -4.0 -0.7 1.0 -0.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.6742 6.2011 5.2562 4.6801 4.1699 5.6563 3.4361 4.0399 3.651 3.5087 3.3638 4.1881 4.0222 4.4633 3.9927 4.1605 3.7392 4.1362
Actin 0.0504 0.0121 0.055 0.1083 0.0803 0.0048 0.0565 0.0084 0.0239 0.0219 0.0366 0.0062 0.0259 0.0135 0.0371 0.011 0.0187 0.0057
Bud 0.0096 0.011 0.109 0.0753 0.0127 0.0017 0.0067 0.0125 0.1187 0.0747 0.0521 0.0037 0.0052 0.0404 0.1512 0.0715 0.0195 0.0029
Bud Neck 0.0158 0.0167 0.0049 0.0037 0.0109 0.3911 0.019 0.0126 0.0069 0.0344 0.0123 0.34 0.0084 0.0101 0.01 0.0117 0.0281 0.2393
Bud Periphery 0.0215 0.0095 0.1771 0.3259 0.0182 0.0033 0.0141 0.0093 0.1618 0.1732 0.1193 0.0089 0.0079 0.0243 0.1819 0.1386 0.0349 0.0034
Bud Site 0.0165 0.1767 0.0578 0.0067 0.0109 0.0044 0.031 0.1142 0.0457 0.0092 0.0095 0.0062 0.0071 0.1301 0.0373 0.0086 0.016 0.0018
Cell Periphery 0.0078 0.0189 0.0061 0.0155 0.0028 0.0019 0.0017 0.0019 0.0043 0.0046 0.0077 0.0016 0.0007 0.0025 0.004 0.0063 0.0039 0.0007
Cytoplasm 0.1121 0.3196 0.3683 0.3347 0.4615 0.1361 0.0842 0.403 0.4561 0.4379 0.4211 0.1577 0.0502 0.3146 0.3555 0.5457 0.4104 0.0852
Cytoplasmic Foci 0.0233 0.0154 0.0076 0.0069 0.0133 0.0083 0.0162 0.0057 0.012 0.0062 0.0039 0.0036 0.0134 0.0043 0.0069 0.0024 0.0239 0.004
Eisosomes 0.0012 0.0001 0.0008 0.0007 0.0002 0.0001 0.0014 0 0.0001 0.0001 0.0001 0 0.0002 0.0001 0.0001 0 0.0001 0
Endoplasmic Reticulum 0.0132 0.0094 0.0082 0.0033 0.0072 0.0039 0.0108 0.0041 0.0065 0.014 0.0101 0.0032 0.0037 0.0028 0.0096 0.0155 0.0116 0.0034
Endosome 0.028 0.0127 0.0043 0.0053 0.0274 0.0142 0.0394 0.0033 0.0134 0.0291 0.0124 0.0098 0.0085 0.0013 0.009 0.0039 0.0171 0.0031
Golgi 0.0075 0.0027 0.0025 0.0081 0.0059 0.0025 0.0116 0.0007 0.0048 0.0063 0.0022 0.0032 0.0035 0.0004 0.0015 0.0004 0.0071 0.0006
Lipid Particles 0.0189 0.0016 0.002 0.0397 0.0071 0.0167 0.009 0.0002 0.001 0.0017 0.0009 0.005 0.0061 0.0002 0.0009 0.0002 0.0118 0.0005
Mitochondria 0.0318 0.0006 0.0155 0.0092 0.0793 0.0132 0.0156 0.0008 0.0225 0.0318 0.0414 0.0012 0.0078 0.0012 0.0024 0.0031 0.0058 0.0007
None 0.0914 0.0457 0.0715 0.0031 0.0024 0.0016 0.0177 0.0033 0.0047 0.0038 0.0022 0.0006 0.0062 0.0091 0.0104 0.0026 0.0263 0.0009
Nuclear Periphery 0.0205 0.0054 0.005 0.003 0.006 0.0089 0.0281 0.009 0.0073 0.0145 0.019 0.0052 0.0109 0.007 0.0119 0.0143 0.014 0.011
Nucleolus 0.0072 0.0029 0.0013 0.0004 0.0012 0.0074 0.003 0.0023 0.0015 0.001 0.0029 0.004 0.0075 0.0027 0.0027 0.0012 0.0034 0.0058
Nucleus 0.4777 0.3162 0.0754 0.0052 0.1726 0.3631 0.6121 0.395 0.0805 0.1116 0.2263 0.4305 0.7937 0.4176 0.1316 0.1364 0.2922 0.6166
Peroxisomes 0.0079 0.0013 0.0077 0.0242 0.0698 0.0033 0.0055 0.0005 0.0019 0.0008 0.0004 0.0007 0.0061 0.0009 0.0012 0.0002 0.0194 0.0002
Punctate Nuclear 0.0135 0.0078 0.0017 0.0031 0.0025 0.0055 0.0051 0.0044 0.0023 0.0056 0.0095 0.0035 0.0208 0.0072 0.0061 0.0015 0.022 0.0096
Vacuole 0.0196 0.0132 0.0166 0.0159 0.0064 0.0071 0.0088 0.0083 0.0209 0.0148 0.0092 0.0048 0.0046 0.0089 0.0261 0.0227 0.0127 0.0043
Vacuole Periphery 0.0046 0.0005 0.0016 0.0017 0.0014 0.0009 0.0026 0.0004 0.0029 0.0029 0.0012 0.0005 0.0016 0.0007 0.0026 0.0022 0.0011 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 16.7769 19.6122 19.4041 24.348 17.0112 20.2071 24.1069 24.537 22.8765 26.3009
Translational Efficiency 0.7819 0.8126 0.7651 0.6451 0.8733 0.7822 0.75 0.6945 0.7905 0.6782

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1895 669 327 1226 1228 1483 1012 2917 3123 2152 1339 4143

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 877.31 1082.77 1466.22 1134.28 926.31 980.42 1159.88 1139.49 896.58 1012.24 1234.69 1137.95
Standard Deviation 112.82 163.38 174.12 153.09 119.38 148.06 150.99 157.92 117.90 160.15 204.83 156.53
Intensity Change Log 2 — 0.303568 0.740943 0.370618 — 0.081905 0.324409 0.298821 — 0.193981 0.542026 0.334191

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.002098 0.004737 0.026827 0.036304 0.004231 0.002244 0.008776 0.011562 0.002937 0.003019 0.013184 0.018884
Bud Neck 0.124880 0.099808 0.141694 0.057613 0.113717 0.098450 0.138646 0.076199 0.120491 0.098872 0.139390 0.070699
Bud Site 0.203375 0.243756 0.352453 0.500023 0.236076 0.177750 0.342711 0.359878 0.216233 0.198269 0.345090 0.401350
Cell Periphery 0.008385 0.003536 0.008788 0.006188 0.007895 0.006481 0.006512 0.003232 0.008192 0.005566 0.007068 0.004107
Cytoplasm 0.233326 0.249088 0.042097 0.309158 0.249599 0.224034 0.129074 0.474042 0.239725 0.231822 0.107833 0.425250
Cytoplasmic Foci 0.014166 0.024257 0.007168 0.008576 0.032477 0.020538 0.004922 0.002908 0.021366 0.021694 0.005471 0.004585
Eisosomes 0.000250 0.000226 0.001577 0.000710 0.000383 0.000247 0.000484 0.000267 0.000302 0.000241 0.000751 0.000398
Endoplasmic Reticulum 0.008799 0.018048 0.044626 0.003310 0.018468 0.015391 0.017727 0.001147 0.012601 0.016217 0.024296 0.001787
Endosome 0.001632 0.003153 0.003141 0.001586 0.003017 0.003281 0.001534 0.000224 0.002176 0.003241 0.001926 0.000627
Golgi 0.000364 0.000970 0.000319 0.000753 0.000954 0.000681 0.000129 0.000143 0.000596 0.000771 0.000175 0.000323
Lipid Particles 0.000547 0.000796 0.004013 0.000676 0.001352 0.000858 0.001024 0.000138 0.000863 0.000838 0.001754 0.000298
Mitochondria 0.001534 0.004229 0.003102 0.001404 0.001149 0.002434 0.001913 0.001193 0.001383 0.002992 0.002204 0.001256
Mitotic Spindle 0.000822 0.008349 0.002085 0.003604 0.000833 0.003986 0.003796 0.002262 0.000826 0.005342 0.003378 0.002659
None 0.003719 0.001370 0.000265 0.000554 0.001886 0.002427 0.003932 0.000491 0.002998 0.002098 0.003037 0.000509
Nuclear Periphery 0.000158 0.000441 0.001165 0.000050 0.000224 0.000351 0.000539 0.000032 0.000184 0.000379 0.000692 0.000037
Nuclear Periphery Foci 0.000238 0.000556 0.001239 0.000379 0.001345 0.000540 0.001132 0.000045 0.000673 0.000545 0.001158 0.000144
Nucleolus 0.000877 0.000465 0.000257 0.000071 0.001127 0.001022 0.000662 0.000042 0.000976 0.000849 0.000563 0.000050
Nucleus 0.361645 0.276443 0.236953 0.027135 0.285133 0.392128 0.257105 0.037681 0.331560 0.356164 0.252184 0.034560
Peroxisomes 0.002636 0.002188 0.001733 0.002653 0.002951 0.003055 0.000883 0.000783 0.002760 0.002786 0.001091 0.001336
Vacuole 0.030239 0.055080 0.120257 0.039021 0.036539 0.043519 0.078305 0.027386 0.032716 0.047113 0.088550 0.030829
Vacuole Periphery 0.000311 0.002503 0.000241 0.000230 0.000643 0.000583 0.000194 0.000345 0.000441 0.001180 0.000205 0.000311

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.23 -7.74 -11.33 -10.03 -3.90 3.45 -2.90 -6.55 -9.38 -2.36 -0.16 -7.47 -13.70 -13.49 -4.87
Bud Neck 3.28 1.08 12.41 5.75 7.20 1.92 -0.29 5.92 4.51 6.70 4.08 0.79 12.18 6.81 9.28
Bud Site -2.21 -1.16 -20.31 -13.57 -11.67 5.47 -2.48 -9.11 -17.18 -5.39 2.85 -4.17 -20.28 -21.54 -10.69
Cell Periphery 4.96 1.56 2.92 -2.48 1.46 1.46 2.69 6.05 4.82 3.51 3.57 3.43 7.21 3.17 3.52
Cytoplasm -1.05 17.08 -8.97 -6.01 -23.50 2.17 9.14 -21.52 -25.23 -35.03 0.98 14.37 -25.98 -24.43 -39.85
Cytoplasmic Foci -3.29 2.88 2.71 4.68 -0.71 4.11 10.67 12.01 9.28 2.89 -0.07 11.00 12.91 10.32 1.50
Eisosomes 0.73 -6.73 -5.52 -5.63 3.27 3.65 -1.16 2.95 -0.43 4.61 2.88 -7.11 -2.39 -4.48 4.76
Endoplasmic Reticulum -4.87 -9.33 5.91 7.28 10.67 1.80 0.16 11.10 14.49 15.25 -3.24 -8.08 14.12 15.68 17.70
Endosome -3.00 -5.59 0.58 2.84 4.57 -0.50 4.47 10.90 7.65 9.23 -3.02 -0.02 8.65 8.04 8.70
Golgi -1.65 0.39 -1.15 0.78 -1.27 1.34 4.59 4.59 4.43 0.28 -0.98 5.38 3.30 3.25 -1.29
Lipid Particles -3.29 -11.93 -3.29 -0.84 10.48 2.99 0.39 9.06 6.22 15.63 0.04 -10.54 6.26 5.12 14.97
Mitochondria -1.93 -4.31 0.41 2.09 4.15 -2.20 -2.49 -0.01 2.21 2.54 -2.54 -2.92 0.58 3.05 4.21
Mitotic Spindle -3.02 -2.88 -2.29 2.07 1.07 -2.96 -3.88 -2.96 1.31 2.06 -4.21 -4.65 -3.66 2.39 2.29
None 7.61 12.14 8.67 2.99 -0.28 -1.45 -1.11 5.28 6.86 2.31 3.29 0.55 11.99 7.52 2.28
Nuclear Periphery -5.12 -14.02 6.32 6.43 14.68 -3.58 -10.63 9.08 9.07 14.95 -6.79 -17.31 12.14 11.10 20.08
Nuclear Periphery Foci -3.31 -8.07 -3.68 0.55 6.61 2.93 -0.23 5.12 4.77 7.58 0.87 -5.14 4.65 4.56 9.39
Nucleolus 3.17 4.38 6.81 6.64 6.05 0.25 1.30 2.85 5.98 3.49 0.62 2.19 5.58 6.99 4.03
Nucleus 4.73 3.01 35.58 18.28 15.70 -7.57 -0.69 22.44 33.16 23.70 -2.70 3.16 41.27 37.50 28.44
Peroxisomes 0.96 2.75 0.48 -0.40 -1.97 -0.13 4.11 5.13 4.23 1.15 0.01 4.83 4.57 3.66 -0.56
Vacuole -7.06 -13.58 -10.31 -2.21 9.51 -2.68 -13.34 -1.10 1.97 13.19 -7.34 -19.05 -8.65 0.09 15.65
Vacuole Periphery -2.19 1.11 1.51 2.31 0.88 0.35 3.36 1.68 1.45 -0.78 -2.26 4.03 1.38 2.69 -0.61
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Guanine nucleotide exchange factor (GEF) for Cdc42p; required for polarity establishment and maintenance, and mutants have morphological defects in bud formation and shmooing; relocalizes from nucleus to cytoplasm upon DNA replication stress; thermosensitivity of the cdc24-4 mutant in the presence of sorbitol is functionally complemented by human CDC42
Localization
Cell Percentages cytoplasm (28%), nucleus (10%), mixed (18%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Cdc24

Cdc24


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cdc24-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available