Standard name
Description Guanine nucleotide exchange factor (GEF) for Cdc42p; required for polarity establishment and maintenance, and mutants have morphological defects in bud formation and shmooing; relocalizes from nucleus to cytoplasm upon DNA replication stress; thermosensitivity of the cdc24-4 mutant in the presence of sorbitol is functionally complemented by human CDC42
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.08 0.06 0.05 0.09 0.12 0.07 0 0.05 0 0 0 0.05 0.14 0.12 0.08 0.15 0.13 0.16 0.08 0.05 0.05 0 0 0
Bud 0.07 0.07 0.07 0.05 0.08 0.07 0.07 0.05 0.07 0 0.08 0.09 0.14 0.07 0.06 0.07 0.05 0.09 0.09 0.14 0.14 0.17 0.15 0.14
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0.05 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0.13 0.17 0.06 0.05 0.06 0 0 0 0 0 0
Cytoplasm 0.33 0.33 0.4 0.42 0.56 0.49 0.53 0.5 0.46 0.46 0.42 0.46 0.37 0.55 0.67 0.48 0.49 0.55 0.19 0.2 0.23 0.06 0 0
Endoplasmic Reticulum 0 0 0.06 0.05 0.05 0.05 0 0 0 0 0 0 0.12 0.1 0.11 0.06 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.06 0 0 0 0.08 0.15 0.06 0.33 0.21 0.35 0.19 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.41 0.31 0.34 0.32 0.17 0.19 0.17 0.14 0.18 0.21 0.17 0.13 0.22 0.13 0.05 0.21 0.15 0.13 0.27 0.23 0.23 0.24 0.23 0.24
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.1 0.24 0.15 0.14 0.11 0.13 0.11 0.2 0.09 0.16 0.12 0.16 0.07 0.08 0.05 0.13 0.19 0.14 0.21 0.27 0.2 0.32 0.39 0.41
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 15 13 11 24 35 24 22 28 17 4 7 17 20 29 21 44 20 18 17 14 11 11 13 5
Bud 13 15 15 13 22 22 33 28 30 15 28 32 21 17 16 19 7 10 20 35 34 45 59 62
Bud Neck 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0 8 13 14 13 17 9
Bud Site 0 1 1 1 1 2 3 8 10 1 12 6 2 0 0 2 1 0 – – – – – –
Cell Periphery 2 2 9 4 10 6 9 4 8 4 9 12 5 32 44 17 7 7 0 1 1 0 0 0
Cytoplasm 60 66 84 110 162 160 262 262 183 159 141 164 55 133 174 138 75 62 43 52 55 16 8 10
Endoplasmic Reticulum 6 3 13 12 13 17 21 23 11 6 6 8 18 23 29 17 6 2 4 1 4 10 15 14
Endosome 1 1 0 0 2 11 6 5 3 4 6 6 3 2 2 3 1 0 5 4 5 6 5 10
Golgi 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0 3 2 3 3 0 0 0 0 3
Mitochondria 6 13 2 7 4 27 73 29 132 72 118 70 6 1 1 2 3 5 1 2 8 7 10 14
Nucleus 75 62 70 82 49 62 85 73 72 74 58 46 32 32 13 61 23 15 61 62 55 64 89 105
Nuclear Periphery 0 0 0 0 0 0 0 3 0 2 0 0 0 0 1 0 0 0 1 0 0 2 8 9
Nucleolus 0 1 0 0 0 1 0 3 2 0 2 2 0 0 0 0 2 0 0 0 0 0 2 3
Peroxisomes 0 0 0 0 1 1 1 2 0 0 1 0 1 1 1 1 0 1 0 0 0 0 0 1
SpindlePole 0 1 1 2 6 1 4 12 3 0 2 13 1 0 0 5 2 4 1 2 1 2 2 7
Vac/Vac Membrane 18 49 32 37 32 44 53 106 36 54 41 57 10 20 12 38 30 16 47 71 49 87 153 181
Unique Cell Count 182 201 208 260 288 327 499 520 400 347 336 359 148 242 260 288 154 113 223 264 247 274 393 447
Labelled Cell Count 197 227 238 292 337 378 572 588 507 395 431 433 174 290 314 350 180 143 223 264 247 274 393 447


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.9 7.7 7.8 7.3 7.8 6.4 6.3 6.6 5.5 5.5 5.2 6.0 8.4 8.5 8.4 10.1 10.0 9.5 8.2 8.2 8.8
Std Deviation (1e-4) 1.5 1.3 1.1 1.1 1.3 1.1 1.0 1.3 1.3 1.1 1.0 1.1 1.4 1.2 1.3 1.6 1.7 1.6 1.1 1.2 1.6
Intensity Change (Log2) – – – -0.09 0.0 -0.3 -0.3 -0.23 -0.49 -0.5 -0.58 -0.38 0.11 0.13 0.11 0.37 0.36 0.29 0.07 0.08 0.17


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 1.6 2.6 0.9 -0.5 0.1 -0.6 -2.9 -2.0 -0.3 2.7 2.5 1.2 3.5 2.6 3.2
Bud -1.0 0.2 -0.2 -0.3 -0.9 0.1 -1.5 0.5 0.7 2.2 -0.1 -0.5 -0.3 -1.0 0.5
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -1.8 -0.5 -1.7 -1.9 0 -1.7 0 -1.0 -0.6 -0.5 3.3 4.3 0.8 0.1 0.7
Cytoplasm 0.4 3.5 1.9 2.9 2.4 1.3 1.2 0.4 1.2 -0.6 3.1 5.7 1.7 1.6 2.5
Endoplasmic Reticulum -0.8 -0.9 -0.5 -1.2 -1.0 -2.1 -2.8 -2.8 -2.4 1.9 1.3 1.8 -0.2 -1.0 -1.8
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 3.6 5.4 2.8 9.0 6.6 9.3 6.4 0 0 0 0 0 0
Nucleus -0.5 -4.3 -3.8 -4.9 -6.0 -4.3 -3.2 -4.4 -5.9 -2.5 -5.2 -8.1 -3.1 -4.0 -4.0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 2.3 0 0 0 0 0 0
Vacuole -0.3 -1.4 -0.6 -1.8 1.6 -2.4 0.1 -1.1 0.2 -2.5 -2.4 -4.0 -0.7 1.0 -0.3


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cdc24-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available