Standard name | |
---|---|
Description | Guanine nucleotide exchange factor (GEF) for Cdc42p; required for polarity establishment and maintenance, and mutants have morphological defects in bud formation and shmooing; relocalizes from nucleus to cytoplasm upon DNA replication stress; thermosensitivity of the cdc24-4 mutant in the presence of sorbitol is functionally complemented by human CDC42 |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 15 | 13 | 11 | 24 | 35 | 24 | 22 | 28 | 17 | 4 | 7 | 17 | 20 | 29 | 21 | 44 | 20 | 18 | 17 | 14 | 11 | 11 | 13 | 5 |
Bud | 13 | 15 | 15 | 13 | 22 | 22 | 33 | 28 | 30 | 15 | 28 | 32 | 21 | 17 | 16 | 19 | 7 | 10 | 20 | 35 | 34 | 45 | 59 | 62 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 8 | 13 | 14 | 13 | 17 | 9 |
Bud Site | 0 | 1 | 1 | 1 | 1 | 2 | 3 | 8 | 10 | 1 | 12 | 6 | 2 | 0 | 0 | 2 | 1 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 2 | 9 | 4 | 10 | 6 | 9 | 4 | 8 | 4 | 9 | 12 | 5 | 32 | 44 | 17 | 7 | 7 | 0 | 1 | 1 | 0 | 0 | 0 |
Cytoplasm | 60 | 66 | 84 | 110 | 162 | 160 | 262 | 262 | 183 | 159 | 141 | 164 | 55 | 133 | 174 | 138 | 75 | 62 | 43 | 52 | 55 | 16 | 8 | 10 |
Endoplasmic Reticulum | 6 | 3 | 13 | 12 | 13 | 17 | 21 | 23 | 11 | 6 | 6 | 8 | 18 | 23 | 29 | 17 | 6 | 2 | 4 | 1 | 4 | 10 | 15 | 14 |
Endosome | 1 | 1 | 0 | 0 | 2 | 11 | 6 | 5 | 3 | 4 | 6 | 6 | 3 | 2 | 2 | 3 | 1 | 0 | 5 | 4 | 5 | 6 | 5 | 10 |
Golgi | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 2 | 3 | 3 | 0 | 0 | 0 | 0 | 3 |
Mitochondria | 6 | 13 | 2 | 7 | 4 | 27 | 73 | 29 | 132 | 72 | 118 | 70 | 6 | 1 | 1 | 2 | 3 | 5 | 1 | 2 | 8 | 7 | 10 | 14 |
Nucleus | 75 | 62 | 70 | 82 | 49 | 62 | 85 | 73 | 72 | 74 | 58 | 46 | 32 | 32 | 13 | 61 | 23 | 15 | 61 | 62 | 55 | 64 | 89 | 105 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 8 | 9 |
Nucleolus | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 3 | 2 | 0 | 2 | 2 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 2 | 3 |
Peroxisomes | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 2 | 0 | 0 | 1 | 0 | 1 | 1 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 |
SpindlePole | 0 | 1 | 1 | 2 | 6 | 1 | 4 | 12 | 3 | 0 | 2 | 13 | 1 | 0 | 0 | 5 | 2 | 4 | 1 | 2 | 1 | 2 | 2 | 7 |
Vac/Vac Membrane | 18 | 49 | 32 | 37 | 32 | 44 | 53 | 106 | 36 | 54 | 41 | 57 | 10 | 20 | 12 | 38 | 30 | 16 | 47 | 71 | 49 | 87 | 153 | 181 |
Unique Cell Count | 182 | 201 | 208 | 260 | 288 | 327 | 499 | 520 | 400 | 347 | 336 | 359 | 148 | 242 | 260 | 288 | 154 | 113 | 223 | 264 | 247 | 274 | 393 | 447 |
Labelled Cell Count | 197 | 227 | 238 | 292 | 337 | 378 | 572 | 588 | 507 | 395 | 431 | 433 | 174 | 290 | 314 | 350 | 180 | 143 | 223 | 264 | 247 | 274 | 393 | 447 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 8.9 | 7.7 | 7.8 | 7.3 | 7.8 | 6.4 | 6.3 | 6.6 | 5.5 | 5.5 | 5.2 | 6.0 | 8.4 | 8.5 | 8.4 | 10.1 | 10.0 | 9.5 | 8.2 | 8.2 | 8.8 |
Std Deviation (1e-4) | 1.5 | 1.3 | 1.1 | 1.1 | 1.3 | 1.1 | 1.0 | 1.3 | 1.3 | 1.1 | 1.0 | 1.1 | 1.4 | 1.2 | 1.3 | 1.6 | 1.7 | 1.6 | 1.1 | 1.2 | 1.6 |
Intensity Change (Log2) | – | – | – | -0.09 | 0.0 | -0.3 | -0.3 | -0.23 | -0.49 | -0.5 | -0.58 | -0.38 | 0.11 | 0.13 | 0.11 | 0.37 | 0.36 | 0.29 | 0.07 | 0.08 | 0.17 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 1.6 | 2.6 | 0.9 | -0.5 | 0.1 | -0.6 | -2.9 | -2.0 | -0.3 | 2.7 | 2.5 | 1.2 | 3.5 | 2.6 | 3.2 |
Bud | -1.0 | 0.2 | -0.2 | -0.3 | -0.9 | 0.1 | -1.5 | 0.5 | 0.7 | 2.2 | -0.1 | -0.5 | -0.3 | -1.0 | 0.5 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -1.8 | -0.5 | -1.7 | -1.9 | 0 | -1.7 | 0 | -1.0 | -0.6 | -0.5 | 3.3 | 4.3 | 0.8 | 0.1 | 0.7 |
Cytoplasm | 0.4 | 3.5 | 1.9 | 2.9 | 2.4 | 1.3 | 1.2 | 0.4 | 1.2 | -0.6 | 3.1 | 5.7 | 1.7 | 1.6 | 2.5 |
Endoplasmic Reticulum | -0.8 | -0.9 | -0.5 | -1.2 | -1.0 | -2.1 | -2.8 | -2.8 | -2.4 | 1.9 | 1.3 | 1.8 | -0.2 | -1.0 | -1.8 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 3.6 | 5.4 | 2.8 | 9.0 | 6.6 | 9.3 | 6.4 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | -0.5 | -4.3 | -3.8 | -4.9 | -6.0 | -4.3 | -3.2 | -4.4 | -5.9 | -2.5 | -5.2 | -8.1 | -3.1 | -4.0 | -4.0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.3 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -0.3 | -1.4 | -0.6 | -1.8 | 1.6 | -2.4 | 0.1 | -1.1 | 0.2 | -2.5 | -2.4 | -4.0 | -0.7 | 1.0 | -0.3 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|