Standard name
Description Putative glycoside hydrolase of the mitochondrial intermembrane space
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0.05 0 0 0 0 0 0
Cytoplasm 0.93 0.95 0.95 0.98 0.97 0.91 0.85 0.86 0.81 0.76 0.68 0.77 0.99 0.99 1.0 0.97 0.97 0.93 0.8 0.87 0.81 0.83 0.81 0.75
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.13 0.09 0.05 0.1 0.05 0.22 0.27 0.23 0.32 0.36 0.49 0.42 0 0 0 0 0 0 0.07 0.07 0.09 0.06 0.07 0.06
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 0 0 0 0 1 3 0 0 0 0 0 0 0 2 0 4 2 4 5
Bud 1 0 4 1 2 2 3 8 5 3 10 12 1 1 0 0 0 0 0 0 0 8 11 19
Bud Neck 0 0 1 0 4 1 1 6 0 0 0 2 0 0 0 0 0 0 1 1 1 1 2 4
Bud Site 0 0 0 0 0 0 0 1 1 0 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 1 2 0 1 2 5 2 2 8 4 8 1 1 0 17 4 8 0 0 2 1 3 6
Cytoplasm 173 227 179 157 251 225 356 374 244 316 203 280 356 622 691 277 204 139 150 205 160 292 405 341
Endoplasmic Reticulum 1 1 2 0 1 2 3 0 3 4 6 1 2 1 0 18 10 4 0 1 0 2 1 2
Endosome 1 0 0 0 0 0 0 2 0 0 0 0 0 1 1 1 2 1 4 1 1 5 3 5
Golgi 1 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 4 0 0 0 4 0
Mitochondria 24 22 9 16 12 53 112 100 95 152 145 155 4 2 1 12 5 2 12 16 18 20 33 29
Nucleus 0 0 0 0 0 1 0 2 1 2 4 2 2 2 1 3 1 5 0 1 0 0 0 0
Nuclear Periphery 1 0 0 0 0 0 1 1 0 4 1 0 0 0 0 0 0 1 0 0 0 0 1 0
Nucleolus 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
SpindlePole 1 0 1 0 0 0 4 3 0 1 1 1 1 0 0 0 0 0 1 0 0 5 10 7
Vac/Vac Membrane 0 0 0 0 0 0 8 20 10 11 5 10 1 1 1 9 2 4 2 2 3 3 6 17
Unique Cell Count 186 239 188 161 258 246 418 437 300 418 298 366 361 628 694 286 210 149 188 238 197 352 500 453
Labelled Cell Count 205 251 198 174 272 286 493 519 361 502 383 472 368 631 695 337 228 164 188 238 197 352 500 453


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.4 3.2 3.3 3.3 3.4 3.4 3.0 3.4 3.7 3.7 4.1 4.1 4.1 4.0 4.0 5.9 6.9 7.3 4.1 4.0 4.5
Std Deviation (1e-4) 1.0 0.7 0.7 1.2 1.0 1.4 1.1 1.1 1.3 1.5 1.5 1.6 1.4 1.4 1.3 1.7 1.8 1.4 1.7 1.6 1.8
Intensity Change (Log2) – – – 0.02 0.07 0.04 -0.13 0.05 0.17 0.18 0.34 0.32 0.33 0.29 0.3 0.86 1.07 1.17 0.32 0.28 0.46


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –