ORF
Description Protein of unknown function found in the cytoplasm and bud neck; mRNA expression may be regulated by the cell cycle and/or cell wall stress; overexpression of YBR071W affects endocytic protein trafficking
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Cytoplasm 0.97 0.98 1.0 0.97 0.96 0.94 0.95 – 0.91 0.81 0.67 0.81 0.99 1.0 1.0 0.98 0.97 0.98 0.9 0.9 0.8 0.92 0.85 0.86
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.15 0 – 0.06 0.29 0.52 0.28 0 0 0 0 0 0 0 0 0.06 0 0.06 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.07 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 1 1 0 0 3 4
Bud 0 0 0 0 0 0 0 – 0 2 5 1 0 1 0 1 1 0 1 1 0 1 5 5
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 1 0 0 0 1 0 1 2
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 2 0 2 2 0 2 – 0 3 1 1 5 1 2 2 8 14 0 0 0 1 3 1
Cytoplasm 192 152 16 90 78 50 157 – 29 85 44 90 325 467 504 224 220 247 180 140 33 212 302 341
Endoplasmic Reticulum 0 1 0 0 0 0 0 – 0 0 0 0 0 0 0 9 5 17 0 1 0 0 1 0
Endosome 1 0 0 0 0 0 1 – 1 0 0 1 0 0 0 1 0 1 1 3 0 1 2 3
Golgi 0 1 0 1 0 0 0 – 0 0 0 0 1 0 0 0 0 0 1 2 0 0 2 1
Mitochondria 6 1 0 2 0 8 6 – 2 30 34 31 1 1 1 5 0 3 7 1 2 7 21 15
Nucleus 0 0 0 0 0 0 0 – 0 1 1 0 0 0 1 1 2 2 0 0 0 0 1 0
Nuclear Periphery 0 1 0 1 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 1 1 – 1 2 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 1 0 0 – 0 0 0 2 0 0 0 0 0 1 0 0 0 0 0 0
SpindlePole 0 0 0 0 1 0 0 – 1 0 0 0 0 0 0 0 0 0 0 0 0 2 2 5
Vac/Vac Membrane 3 0 0 2 6 0 5 – 0 0 0 1 1 2 0 1 4 4 0 0 0 2 2 4
Unique Cell Count 198 155 16 93 81 53 166 32 105 66 111 328 469 505 229 226 252 202 156 42 232 354 399
Labelled Cell Count 204 158 16 98 88 59 172 34 123 85 127 333 472 508 245 241 289 202 156 42 232 354 399


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.5 4.7 7.5 5.2 8.8 3.8 5.2 – 5.3 4.4 4.1 4.7 5.2 5.1 5.2 5.6 5.8 6.0 4.0 4.2 4.2
Std Deviation (1e-4) 0.9 1.1 4.2 1.5 12.7 1.0 1.7 – 3.2 2.3 1.8 2.3 1.3 1.3 1.6 1.3 1.1 1.6 0.8 1.7 1.7
Intensity Change (Log2) – – – -0.54 0.23 -0.96 -0.53 – -0.5 -0.77 -0.85 -0.66 -0.52 -0.55 -0.51 -0.41 -0.37 -0.33 -0.91 -0.83 -0.84


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -1.0535 0.7545 -0.0631 -1.2383 -1.3205 -0.1205 0.7208 2.5292 1.919 0.8502 0.9713 1.8058 2.3909 4.6539 3.493 3.152 2.6428 3.7678
Actin 0.0151 0.0016 0.007 0.0005 0.0006 0.0013 0.0339 0.0047 0.0117 0.0071 0.0045 0.0176 0.0327 0.0019 0.0082 0.0209 0.0685 0.0063
Bud 0.0007 0.0006 0.0002 0.0002 0.0018 0.0004 0.0016 0.0036 0.0008 0.0024 0.0009 0.0004 0.0008 0.0009 0.0003 0.0046 0.0013 0.0006
Bud Neck 0.0018 0.0002 0.0008 0.0002 0.0034 0.0034 0.0024 0.0002 0.0009 0.0022 0.0006 0.0044 0.0045 0.0002 0.0015 0.0089 0.0007 0.0007
Bud Periphery 0.0013 0.0008 0.0003 0.0002 0.0051 0.0004 0.0031 0.0029 0.001 0.0045 0.0011 0.0005 0.0013 0.0004 0.0003 0.007 0.0038 0.0006
Bud Site 0.0022 0.0022 0.0017 0.0002 0.0027 0.0022 0.0121 0.004 0.0135 0.0023 0.008 0.0002 0.0055 0.001 0.0089 0.0153 0.0097 0.0017
Cell Periphery 0.0002 0.0002 0.0002 0 0.0003 0.0004 0.0004 0.0002 0.0002 0.0003 0.0001 0.0001 0.0007 0.0002 0.0002 0.0009 0.0012 0.0001
Cytoplasm 0.2161 0.5176 0.3756 0.1885 0.1956 0.4796 0.3716 0.7196 0.621 0.3854 0.3409 0.6598 0.3583 0.661 0.5214 0.2741 0.1971 0.621
Cytoplasmic Foci 0.0231 0.0104 0.0094 0.0223 0.0246 0.0194 0.0282 0.0083 0.0102 0.0323 0.0394 0.0124 0.0126 0.0041 0.0122 0.014 0.0077 0.0075
Eisosomes 0.0004 0.0001 0.0002 0.0001 0.0001 0.0001 0.0004 0.0001 0.0002 0.0001 0.0001 0.0001 0.0007 0 0.0002 0.0002 0.0009 0.0001
Endoplasmic Reticulum 0.0024 0.0029 0.0021 0.0017 0.0012 0.0016 0.0036 0.0019 0.0015 0.0028 0.0013 0.0017 0.0047 0.0014 0.0019 0.0058 0.0029 0.0018
Endosome 0.0084 0.0074 0.0068 0.0049 0.0048 0.0046 0.0235 0.0078 0.0066 0.0317 0.03 0.0035 0.0084 0.0012 0.0051 0.0276 0.0186 0.01
Golgi 0.0035 0.0005 0.0016 0.0002 0.0012 0.0004 0.0095 0.002 0.0044 0.0122 0.0213 0.0021 0.0044 0.0002 0.0022 0.005 0.0155 0.0027
Lipid Particles 0.0046 0.0004 0.004 0.0007 0.0047 0.0011 0.0083 0.0005 0.0027 0.0303 0.0496 0.0031 0.0025 0.0001 0.0024 0.0075 0.0373 0.0031
Mitochondria 0.0023 0.0008 0.001 0.0003 0.0078 0.0005 0.0143 0.0025 0.0062 0.0079 0.0076 0.0006 0.0112 0.0004 0.0148 0.0062 0.0123 0.0011
None 0.7013 0.4467 0.5796 0.7727 0.7079 0.4779 0.4558 0.238 0.3063 0.4561 0.4547 0.2838 0.5303 0.3122 0.4062 0.5844 0.6004 0.3328
Nuclear Periphery 0.0025 0.0009 0.0019 0.001 0.0012 0.0009 0.0073 0.0005 0.0007 0.0026 0.0014 0.0007 0.0024 0.0007 0.0033 0.0038 0.0073 0.0017
Nucleolus 0.0004 0.0002 0.0005 0.0002 0.0013 0.0002 0.0007 0.0001 0.0003 0.0004 0.0005 0.0002 0.0009 0.0011 0.0006 0.0005 0.0004 0.0002
Nucleus 0.0016 0.0018 0.0026 0.0016 0.0108 0.0016 0.0027 0.0012 0.0015 0.0034 0.0026 0.0029 0.0022 0.0109 0.0034 0.0018 0.0009 0.0007
Peroxisomes 0.0068 0.0003 0.0008 0.0003 0.0158 0.0008 0.0058 0.0005 0.0026 0.0041 0.0295 0.0023 0.0043 0.0002 0.0039 0.004 0.0078 0.0032
Punctate Nuclear 0.0036 0.0012 0.0023 0.0029 0.0069 0.0019 0.0069 0.0003 0.0064 0.0032 0.0031 0.003 0.0098 0.0013 0.0023 0.0053 0.0022 0.0035
Vacuole 0.0013 0.0027 0.0012 0.001 0.002 0.0012 0.0055 0.001 0.0011 0.0068 0.0015 0.0005 0.0012 0.0005 0.0006 0.0015 0.0017 0.0004
Vacuole Periphery 0.0003 0.0004 0.0003 0.0002 0.0003 0.0002 0.0023 0.0002 0.0002 0.002 0.0013 0.0001 0.0004 0.0001 0.0002 0.0008 0.0018 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 40.7704 15.5091 24.1676 87.9088 35.9902 69.3581 10.5848 8.9962 63.257 60.7839
Translational Efficiency 1.7142 0.8798 1.7805 1.735 2.5549 1.8998 2.2257 2.0967 2.05 2.0834

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘