Standard name
Description Small heat shock protein (sHSP) with chaperone activity; forms hollow, sphere-shaped oligomers that suppress unfolded proteins aggregation; long-lived protein that is preferentially retained in mother cells and forms cytoplasmic foci; oligomer activation requires heat-induced conformational change; also has mRNA binding activity

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.97 0.93 0.99 0.98 0.97 0.88 0.82 0.7 0.6 0.48 0.48 0.97 0.97 0.99 0.95 0.89 0.91 0.82 0.88 0.84 0.85 0.82 0.78
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.08 0.05 0 0 0 0.22 0.06 0.15 0.12 0.26 0.18 0 0 0 0 0.05 0.05 0.12 0 0.05 0 0 0.05
Nucleus 0 0 0 0 0 0 0 0 0 0.05 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.13 0.19 0.21 0.24 0.23 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 0 1 1 3 3 4 0 0 0 1 0 0 0 0 0 0 1 2
Bud 0 1 0 0 0 5 3 8 11 15 6 0 0 0 1 0 0 0 1 0 2 8 7
Bud Neck 1 0 0 3 3 0 2 0 1 0 0 0 0 0 0 0 0 1 0 0 1 2 1
Bud Site 0 0 0 0 0 0 2 0 1 0 0 0 0 0 0 0 0
Cell Periphery 0 2 0 0 1 0 1 3 3 4 8 0 1 1 0 0 2 0 0 0 0 0 1
Cytoplasm 144 164 85 177 220 133 230 137 147 102 112 121 176 248 91 49 58 140 158 76 104 203 154
Endoplasmic Reticulum 0 2 0 0 0 0 1 0 3 8 12 1 0 1 4 2 3 0 0 0 1 2 1
Endosome 0 0 1 0 2 1 2 0 2 1 1 0 2 1 3 2 2 1 5 0 1 3 5
Golgi 0 0 0 0 0 0 1 1 3 7 4 0 1 0 1 1 0 0 0 0 0 1 2
Mitochondria 12 9 0 1 1 33 16 30 29 55 41 2 1 0 4 3 3 19 6 4 4 10 9
Nucleus 0 2 0 2 1 4 5 8 8 10 13 1 0 0 1 0 0 0 0 0 0 0 2
Nuclear Periphery 0 0 0 0 0 1 4 2 4 6 7 0 0 0 0 1 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 1 1 2 5 4 6 0 0 0 0 1 0 0 0 0 0 0 1
Peroxisomes 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 1 0 0 3 3 6 4 1 0 0 0 0 0 0 1 0 2 0 3 2
Vac/Vac Membrane 1 2 0 2 7 4 37 37 51 51 54 1 2 1 0 0 1 2 4 0 0 2 2
Unique Cell Count 149 176 86 180 226 152 282 197 246 214 231 125 182 250 96 55 64 172 180 91 123 248 199
Labelled Cell Count 158 183 86 186 235 182 309 233 277 270 269 126 183 252 106 59 69 172 180 91 123 248 199


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.5 4.6 5.3 4.6 5.7 4.6 8.5 16.0 15.5 16.3 19.7 4.7 5.0 6.2 5.4 5.7 5.4 13.0 6.6 12.4
Std Deviation (1e-4) 0.3 1.7 2.8 1.4 2.3 2.5 6.8 19.4 16.4 22.8 23.6 1.7 1.8 3.2 1.3 1.7 1.2 36.7 18.4 41.0
Intensity Change (Log2) -0.18 0.12 -0.2 0.69 1.61 1.56 1.63 1.9 -0.16 -0.07 0.24 0.04 0.11 0.03 1.31 0.33 1.24


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Hsp26-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available