Standard name | |
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Human Ortholog | |
Description | Small heat shock protein (sHSP) with chaperone activity; forms hollow, sphere-shaped oligomers that suppress unfolded proteins aggregation; long-lived protein that is preferentially retained in mother cells and forms cytoplasmic foci; oligomer activation requires heat-induced conformational change; also has mRNA binding activity |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | 0 | 0 | 0 | 0 | 1 | – | 1 | 3 | 3 | 4 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 |
Bud | 0 | 1 | 0 | 0 | 0 | 5 | 3 | – | 8 | 11 | 15 | 6 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 2 | 8 | 7 |
Bud Neck | 1 | 0 | 0 | 3 | 3 | 0 | 2 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 2 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 2 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 2 | 0 | 0 | 1 | 0 | 1 | – | 3 | 3 | 4 | 8 | 0 | 1 | 1 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 1 |
Cytoplasm | 144 | 164 | 85 | 177 | 220 | 133 | 230 | – | 137 | 147 | 102 | 112 | 121 | 176 | 248 | 91 | 49 | 58 | 140 | 158 | 76 | 104 | 203 | 154 |
Endoplasmic Reticulum | 0 | 2 | 0 | 0 | 0 | 0 | 1 | – | 0 | 3 | 8 | 12 | 1 | 0 | 1 | 4 | 2 | 3 | 0 | 0 | 0 | 1 | 2 | 1 |
Endosome | 0 | 0 | 1 | 0 | 2 | 1 | 2 | – | 0 | 2 | 1 | 1 | 0 | 2 | 1 | 3 | 2 | 2 | 1 | 5 | 0 | 1 | 3 | 5 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 1 | 3 | 7 | 4 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 2 |
Mitochondria | 12 | 9 | 0 | 1 | 1 | 33 | 16 | – | 30 | 29 | 55 | 41 | 2 | 1 | 0 | 4 | 3 | 3 | 19 | 6 | 4 | 4 | 10 | 9 |
Nucleus | 0 | 2 | 0 | 2 | 1 | 4 | 5 | – | 8 | 8 | 10 | 13 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 1 | 4 | – | 2 | 4 | 6 | 7 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 1 | 1 | – | 2 | 5 | 4 | 6 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 1 | 0 | 0 | 3 | – | 3 | 6 | 4 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 3 | 2 |
Vac/Vac Membrane | 1 | 2 | 0 | 2 | 7 | 4 | 37 | – | 37 | 51 | 51 | 54 | 1 | 2 | 1 | 0 | 0 | 1 | 2 | 4 | 0 | 0 | 2 | 2 |
Unique Cell Count | 149 | 176 | 86 | 180 | 226 | 152 | 282 | 197 | 246 | 214 | 231 | 125 | 182 | 250 | 96 | 55 | 64 | 172 | 180 | 91 | 123 | 248 | 199 | |
Labelled Cell Count | 158 | 183 | 86 | 186 | 235 | 182 | 309 | 233 | 277 | 270 | 269 | 126 | 183 | 252 | 106 | 59 | 69 | 172 | 180 | 91 | 123 | 248 | 199 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.5 | 4.6 | 5.3 | 4.6 | 5.7 | 4.6 | 8.5 | – | 16.0 | 15.5 | 16.3 | 19.7 | 4.7 | 5.0 | 6.2 | 5.4 | 5.7 | 5.4 | 13.0 | 6.6 | 12.4 |
Std Deviation (1e-4) | 0.3 | 1.7 | 2.8 | 1.4 | 2.3 | 2.5 | 6.8 | – | 19.4 | 16.4 | 22.8 | 23.6 | 1.7 | 1.8 | 3.2 | 1.3 | 1.7 | 1.2 | 36.7 | 18.4 | 41.0 |
Intensity Change (Log2) | – | – | – | -0.18 | 0.12 | -0.2 | 0.69 | – | 1.61 | 1.56 | 1.63 | 1.9 | -0.16 | -0.07 | 0.24 | 0.04 | 0.11 | 0.03 | 1.31 | 0.33 | 1.24 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.2235 | 10.0043 | 3.5928 | 5.2461 | 7.8135 | 2.4988 | 6.7522 | 13.4383 | 7.223 | 8.0801 | 5.7058 | 9.031 | 6.4309 | 11.404 | 7.9538 | 9.5494 | 8.1453 | 10.261 |
Actin | 0.0307 | 0.0314 | 0.0387 | 0.0164 | 0.0243 | 0.0209 | 0.0217 | 0.0145 | 0.0328 | 0.0677 | 0.0397 | 0.0167 | 0.0272 | 0.0365 | 0.019 | 0.0158 | 0.0041 | 0.0307 |
Bud | 0.0123 | 0.0149 | 0.0115 | 0.0065 | 0.0015 | 0.0036 | 0.0069 | 0.0226 | 0.009 | 0.0125 | 0.0014 | 0.0019 | 0.0056 | 0.0127 | 0.0057 | 0.0124 | 0.0078 | 0.0054 |
Bud Neck | 0.0039 | 0.0015 | 0.0012 | 0.001 | 0.0048 | 0.0035 | 0.005 | 0.0016 | 0.0031 | 0.0041 | 0.0012 | 0.0259 | 0.0028 | 0.0028 | 0.0059 | 0.0235 | 0.0029 | 0.0044 |
Bud Periphery | 0.0062 | 0.0086 | 0.0064 | 0.0061 | 0.0011 | 0.0042 | 0.0046 | 0.0097 | 0.0114 | 0.007 | 0.0013 | 0.0016 | 0.0046 | 0.012 | 0.004 | 0.0087 | 0.0069 | 0.0083 |
Bud Site | 0.0217 | 0.0545 | 0.0173 | 0.0024 | 0.0025 | 0.0015 | 0.0143 | 0.036 | 0.0149 | 0.0071 | 0.0039 | 0.0021 | 0.0077 | 0.0447 | 0.0059 | 0.0052 | 0.0036 | 0.0044 |
Cell Periphery | 0.0006 | 0.0012 | 0.0008 | 0.0003 | 0.0002 | 0.0005 | 0.0007 | 0.0016 | 0.0004 | 0.0003 | 0.0003 | 0.0002 | 0.0005 | 0.0012 | 0.0012 | 0.0006 | 0.0002 | 0.0021 |
Cytoplasm | 0.1844 | 0.2625 | 0.2179 | 0.2211 | 0.0805 | 0.2344 | 0.2499 | 0.3792 | 0.2614 | 0.3229 | 0.1636 | 0.2782 | 0.2874 | 0.2683 | 0.2623 | 0.2896 | 0.3412 | 0.2904 |
Cytoplasmic Foci | 0.104 | 0.2061 | 0.1182 | 0.2475 | 0.2116 | 0.1207 | 0.1366 | 0.1601 | 0.1802 | 0.1402 | 0.1354 | 0.1814 | 0.097 | 0.2026 | 0.1687 | 0.1055 | 0.1738 | 0.1533 |
Eisosomes | 0.0003 | 0.0002 | 0.0003 | 0.0013 | 0.0002 | 0.0001 | 0.0004 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0002 |
Endoplasmic Reticulum | 0.0108 | 0.0108 | 0.0231 | 0.0047 | 0.007 | 0.0123 | 0.0241 | 0.012 | 0.0193 | 0.0123 | 0.0254 | 0.0176 | 0.0189 | 0.0138 | 0.0201 | 0.0172 | 0.0097 | 0.0121 |
Endosome | 0.1551 | 0.1584 | 0.127 | 0.1441 | 0.1249 | 0.1641 | 0.1655 | 0.1109 | 0.1727 | 0.1567 | 0.2975 | 0.1796 | 0.1146 | 0.1031 | 0.1425 | 0.0841 | 0.2031 | 0.1489 |
Golgi | 0.0357 | 0.0293 | 0.0351 | 0.0153 | 0.0846 | 0.0421 | 0.0332 | 0.0271 | 0.0411 | 0.0418 | 0.1157 | 0.0337 | 0.025 | 0.0217 | 0.0351 | 0.0073 | 0.0121 | 0.0367 |
Lipid Particles | 0.0239 | 0.0083 | 0.0096 | 0.0345 | 0.0069 | 0.0159 | 0.0141 | 0.0082 | 0.0067 | 0.003 | 0.0264 | 0.0114 | 0.0115 | 0.0069 | 0.0113 | 0.004 | 0.0108 | 0.0058 |
Mitochondria | 0.0215 | 0.0106 | 0.0188 | 0.0059 | 0.0832 | 0.0095 | 0.012 | 0.0091 | 0.0252 | 0.0243 | 0.0383 | 0.0125 | 0.0088 | 0.0072 | 0.0066 | 0.0144 | 0.0049 | 0.0147 |
None | 0.176 | 0.0806 | 0.1671 | 0.149 | 0.3145 | 0.2024 | 0.0552 | 0.0309 | 0.0233 | 0.0466 | 0.005 | 0.0404 | 0.161 | 0.108 | 0.0998 | 0.1502 | 0.0422 | 0.0797 |
Nuclear Periphery | 0.0614 | 0.0102 | 0.0509 | 0.0259 | 0.0033 | 0.0367 | 0.0754 | 0.0165 | 0.0429 | 0.022 | 0.0273 | 0.0424 | 0.0557 | 0.0303 | 0.0416 | 0.0563 | 0.0167 | 0.0348 |
Nucleolus | 0.0047 | 0.0181 | 0.0025 | 0.0073 | 0.0042 | 0.0007 | 0.0085 | 0.0094 | 0.0039 | 0.001 | 0.0011 | 0.0048 | 0.0044 | 0.0195 | 0.0082 | 0.0018 | 0.0116 | 0.0139 |
Nucleus | 0.0742 | 0.05 | 0.1118 | 0.0646 | 0.0023 | 0.0686 | 0.0901 | 0.0838 | 0.0784 | 0.1 | 0.0581 | 0.0729 | 0.1185 | 0.0473 | 0.0996 | 0.1209 | 0.0789 | 0.0817 |
Peroxisomes | 0.0118 | 0.0183 | 0.0156 | 0.0202 | 0.0174 | 0.0152 | 0.0142 | 0.0198 | 0.0261 | 0.01 | 0.0315 | 0.0332 | 0.0071 | 0.0348 | 0.0237 | 0.0332 | 0.011 | 0.0237 |
Punctate Nuclear | 0.0398 | 0.0094 | 0.0148 | 0.0151 | 0.0194 | 0.0256 | 0.0287 | 0.0256 | 0.0275 | 0.0085 | 0.0069 | 0.0244 | 0.0267 | 0.0173 | 0.025 | 0.0336 | 0.0413 | 0.0309 |
Vacuole | 0.0156 | 0.0125 | 0.0077 | 0.0079 | 0.0026 | 0.0136 | 0.0307 | 0.0169 | 0.0149 | 0.0088 | 0.0126 | 0.0134 | 0.0106 | 0.0072 | 0.01 | 0.0096 | 0.0129 | 0.0116 |
Vacuole Periphery | 0.0055 | 0.0027 | 0.0035 | 0.0028 | 0.0033 | 0.0041 | 0.0081 | 0.0046 | 0.0047 | 0.0033 | 0.0073 | 0.0057 | 0.0043 | 0.0018 | 0.0036 | 0.0061 | 0.0045 | 0.0064 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 11.1196 | 4.5878 | 10.213 | 12.6027 | 8.872 | 8.5488 | 8.8909 | 18.7849 | 17.7045 | 14.9157 |
Translational Efficiency | 1.692 | 3.4192 | 1.5295 | 1.6778 | 2.1884 | 2.2362 | 2.4186 | 1.162 | 2.3103 | 1.9065 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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