Standard name
Description Peptidylprolyl-cis/trans-isomerase (PPIase); specific for phosphorylated S/T residues N-terminal to proline; regulates phosphorylation of RNAPII large subunit (Rpo21p) C-terminal domain (CTD) at Ser7; associates with phospho-Ser5 form of RNAPII in vivo; present along entire coding length of genes; represses initiation of CUTs; required for efficient termination of mRNA transcription, trimethylation of histone H3; human ortholog PIN1 can complement yeast null and ts mutants
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0.06
Bud Neck 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.06 – 0 0.05 0.05 0.06 0.09 – 0.05 0.06 0.06 0.05 0.1 0.24 0.32 0.11 0.13 0.18 0 0 0 0 0.05 0.08
Endoplasmic Reticulum 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.05 0.05
Golgi 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.08 0.13 0 0 0 0 0 0
Mitochondria 0 – 0 0 0 0.08 0 – 0.09 0 0.08 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.5 – 0.29 0.76 0.74 0.55 0.71 – 0.65 0.6 0.67 0.68 0.45 0.27 0.18 0.48 0.41 0.3 0.69 0.8 0.63 0.54 0.42 0.43
Nuclear Periphery 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.44 – 0.47 0 0 0.09 0 – 0.09 0.12 0.11 0.1 0.05 0 0 0 0 0 0.22 0.08 0.21 0.18 0.17 0.12
Peroxisomes 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.09
Vac/Vac Membrane 0.17 – 0.29 0.19 0.22 0.31 0.23 – 0.2 0.27 0.16 0.17 0.41 0.5 0.52 0.4 0.47 0.46 0 0 0.06 0.09 0.11 0.1
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 – 0 0 0 0 0 – 0 1 0 2 0 0 0 1 0 0 0 0 0 0 0 0
Bud 0 – 0 0 0 2 1 – 3 3 3 3 0 1 0 1 0 0 1 1 0 21 46 35
Bud Neck 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 9
Bud Site 0 – 0 0 0 0 0 – 4 4 3 3 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2 – 0 11 21 13 33 – 13 21 13 17 30 104 175 17 17 27 0 1 0 10 36 43
Endoplasmic Reticulum 0 – 0 0 0 0 0 – 0 0 0 0 0 1 0 5 5 8 0 0 0 1 4 5
Endosome 0 – 0 5 1 2 3 – 4 6 3 4 7 4 18 3 1 1 1 0 2 11 35 26
Golgi 0 – 0 0 5 0 4 – 0 1 0 0 10 18 17 6 10 19 0 0 0 0 1 3
Mitochondria 0 – 0 0 2 18 1 – 24 13 19 21 1 0 0 1 1 1 0 0 0 1 1 0
Nucleus 18 – 5 156 288 119 273 – 183 205 155 244 133 113 97 72 54 44 50 55 30 188 303 244
Nuclear Periphery 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 1 10 6
Nucleolus 16 – 8 0 7 19 9 – 24 42 26 37 15 6 4 1 2 6 16 5 10 64 126 70
Peroxisomes 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 – 0 0 1 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 1 14 59 49
Vac/Vac Membrane 6 – 5 38 84 66 89 – 56 93 36 61 119 214 289 60 63 68 0 2 2 30 78 58
Unique Cell Count 36 17 204 389 216 387 282 344 231 359 293 426 552 151 133 149 73 70 49 352 724 568
Labelled Cell Count 42 18 210 409 239 413 311 389 258 392 315 461 600 167 153 174 73 70 49 352 724 568


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.4 – 8.9 11.8 12.4 8.0 11.3 – 9.5 10.2 9.5 9.9 14.8 14.7 14.8 18.2 19.8 19.5 12.2 12.4 12.8
Std Deviation (1e-4) 2.5 – 1.3 2.1 1.9 2.0 2.2 – 1.9 1.9 1.8 2.0 2.3 2.2 2.2 4.4 4.7 5.7 1.7 1.9 2.0
Intensity Change (Log2) – – – 0.41 0.49 -0.15 0.35 – 0.1 0.2 0.11 0.16 0.74 0.73 0.74 1.04 1.16 1.14 0.46 0.48 0.53


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 – 0 0 0 0 0 0 2.8 0 0 0
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleus 4.2 4.0 2.0 3.6 – 2.9 2.5 3.1 3.3 1.3 0 0 1.4 0.9 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0.1 0 – 0 0 0 0 0.9 1.7 1.9 0.8 1.4 1.3