Standard name
Human Ortholog
Description AAA ATPase and SNARE disassembly chaperone; required for vesicular transport between ER and Golgi, the 'priming' step in homotypic vacuole fusion, autophagy, and protein secretion; releases Sec17p from SNAP complexes; has similarity to mammalian N-ethylmaleimide-sensitive factor (NSF)
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.22 0.23 0.21 0.22 0.17 0.15 0.16 0.16 – 0.17 0.21 0.18 0.22 0.23 0.14 0.2 0.15 0.17 0.11 0.11 0.21 0.18 0.18 0.12
Bud 0 0 0 0 0.05 0.07 0.07 0.06 – 0.06 0.1 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.29 0.07 0.11 0.14 0.11 0.09 0.09 – 0.06 0.11 0.1 0.26 0.23 0.28 0.21 0.2 0.26 0 0.07 0 0 0 0
Endoplasmic Reticulum 0.3 0.4 0.25 0.3 0.27 0.28 0.23 0.19 – 0.19 0.13 0.25 0.51 0.37 0.23 0 0 0 0.09 0.15 0.08 0.12 0.16 0.13
Endosome 0 0 0.11 0.05 0 0.06 0 0 – 0.05 0 0 0 0 0 0.2 0.27 0.07 0.15 0.19 0.14 0.15 0.1 0.12
Golgi 0.6 0.19 0.53 0.61 0.56 0.3 0.38 0.32 – 0.2 0.15 0.23 0.4 0.44 0.48 0.28 0.23 0.53 0.58 0.31 0.49 0.42 0.46 0.55
Mitochondria 0.07 0.16 0.17 0.3 0.18 0.48 0.45 0.5 – 0.63 0.6 0.57 0 0 0 0.16 0.12 0.07 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.07 0.06 0 0 0 0 0 0
Vac/Vac Membrane 0.16 0 0.06 0 0.05 0 0 0 – 0 0 0 0 0.05 0.07 0.16 0.17 0.2 0 0.09 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 57 46 68 43 46 47 64 82 – 80 49 50 25 53 25 37 21 15 32 29 79 44 55 47
Bud 10 6 11 3 13 23 27 29 – 27 23 19 4 0 2 4 5 1 1 3 2 2 3 3
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 1 0 1 2 0
Bud Site 0 0 0 0 0 0 0 1 – 1 1 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 3 1 1 2 1 5 3 – 1 1 4 0 3 4 0 0 0 0 0 0 0 0 0
Cytoplasm 9 58 24 22 38 34 37 44 – 29 26 27 29 52 49 38 28 23 4 17 0 5 5 5
Endoplasmic Reticulum 79 80 80 58 73 90 92 95 – 87 29 69 57 83 40 4 4 3 27 38 30 29 52 49
Endosome 10 3 35 9 10 18 17 20 – 22 10 3 3 2 4 37 38 6 46 48 53 36 33 45
Golgi 159 38 174 117 150 98 155 162 – 93 35 61 45 100 84 52 32 47 174 80 185 101 144 214
Mitochondria 19 32 57 58 47 154 183 257 – 288 139 154 1 2 0 30 17 6 4 4 4 8 7 8
Nucleus 0 1 0 0 2 0 0 1 – 1 1 0 0 0 1 5 9 1 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 2 0 2 2 – 2 0 3 1 0 0 0 1 0 0 4 0 0 0 0
Nucleolus 2 2 3 1 3 5 8 22 – 11 8 2 0 0 0 4 5 1 0 0 0 0 0 1
Peroxisomes 0 0 4 1 1 0 2 0 – 0 0 1 0 0 0 0 0 0 1 0 4 4 1 2
SpindlePole 0 0 4 2 0 0 0 0 – 0 1 0 0 0 0 6 10 5 0 1 2 0 2 1
Vac/Vac Membrane 42 7 20 0 14 13 17 16 – 14 4 2 4 11 13 30 23 18 3 22 6 7 7 9
Unique Cell Count 263 198 326 193 267 322 408 513 458 230 271 112 226 175 185 139 89 299 257 375 244 318 392
Labelled Cell Count 388 276 481 315 401 483 609 734 656 327 396 169 306 222 247 193 126 299 257 375 244 318 392


Early Golgi, Golgi

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 17.4 14.6 14.3 13.9 15.8 13.9 14.5 13.7 – 13.4 13.0 14.4 15.2 16.2 17.1 11.1 11.0 16.2 15.9 16.6 18.0
Std Deviation (1e-4) 4.2 3.7 3.2 3.1 4.0 3.7 4.2 3.5 – 4.6 4.6 5.2 3.5 3.6 4.5 3.5 3.8 5.4 2.8 3.6 4.2
Intensity Change (Log2) – – – -0.03 0.14 -0.04 0.02 -0.06 – -0.09 -0.14 0.01 0.09 0.19 0.26 -0.36 -0.37 0.18 0.15 0.22 0.34


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0.4 -1.1 -2.1 -1.8 -1.8 – -1.2 0.1 -0.7 0.3 0.7 -1.8 -0.2 -1.4 -0.8
Bud -1.2 0.9 2.2 2.0 1.5 – 1.6 3.2 2.0 0 0 0 -0.8 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cytoplasm 1.6 2.7 1.4 0.8 0.6 – -0.6 1.6 1.1 5.2 5.2 6.2 4.4 4.0 4.9
Endoplasmic Reticulum 1.4 0.8 1.0 -0.6 -2.1 – -1.9 -3.5 0.3 5.2 3.1 -0.4 -6.6 -5.6 -4.4
Endosome -2.4 -3.2 -2.4 -3.4 -3.9 – -3.2 -2.7 -4.8 -2.6 -4.6 -3.4 2.9 4.5 -1.1
Golgi 1.6 0.7 -5.9 -4.2 -6.3 – -9.6 -9.1 -7.7 -2.4 -2.1 -1.1 -5.5 -6.0 -0.1
Mitochondria 3.3 0 8.2 7.9 9.5 – 12.6 10.4 10.0 -4.5 -6.2 -5.9 -0.4 -1.4 -2.5
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 2.8 – 1.5 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Vacuole -3.5 -0.5 -1.2 -1.2 -2.1 – -2.1 -2.5 -3.5 -1.0 -0.6 0.6 3.7 3.5 4.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.1557 0.0989 0.0602 0.0613 0.0717 0.084 0.1405 0.0366 0.0452 0.0664 0.0411 0.0397 – – – – – –
Bud 0.0055 0.0024 0.0171 0.0052 0.0003 0.0017 0.0005 0.0005 0.0146 0.0007 0.0012 0.0002 – – – – – –
Bud Neck 0.0041 0.0015 0.0017 0.0023 0.0014 0.0439 0.0013 0.0004 0.0012 0.0008 0.0009 0.0137 – – – – – –
Bud Periphery 0.0085 0.0017 0.0195 0.0061 0.0002 0.0009 0.0007 0.0002 0.0131 0.0004 0.0019 0.0002 – – – – – –
Bud Site 0.0115 0.0187 0.0119 0.0019 0.0003 0.0005 0.0024 0.0103 0.0075 0.0063 0.0006 0.0002 – – – – – –
Cell Periphery 0.0062 0.0008 0.0012 0.001 0.0002 0.0005 0.0006 0.0002 0.0005 0.0002 0.0008 0.0015 – – – – – –
Cytoplasm 0.1053 0.0477 0.0583 0.0254 0.017 0.0807 0.0101 0.0262 0.0196 0.0763 0.0308 0.0144 – – – – – –
Cytoplasmic Foci 0.0338 0.0619 0.0653 0.0767 0.1276 0.0493 0.0375 0.0424 0.0315 0.0311 0.0328 0.029 – – – – – –
Eisosomes 0.0013 0.0002 0.0001 0.0002 0.0001 0.0002 0.002 0 0.0002 0.0002 0.0001 0.0002 – – – – – –
Endoplasmic Reticulum 0.0317 0.003 0.0107 0.0043 0.0023 0.0096 0.0231 0.005 0.0057 0.0033 0.0117 0.0037 – – – – – –
Endosome 0.1648 0.2452 0.27 0.238 0.1862 0.1331 0.1359 0.2001 0.1861 0.2188 0.1859 0.106 – – – – – –
Golgi 0.356 0.4735 0.4051 0.459 0.5596 0.5528 0.5937 0.6495 0.6446 0.5583 0.6012 0.7697 – – – – – –
Lipid Particles 0.0255 0.0213 0.0178 0.0653 0.0246 0.0073 0.015 0.015 0.0061 0.0153 0.0219 0.0084 – – – – – –
Mitochondria 0.0079 0.0128 0.0159 0.0058 0.0029 0.0146 0.0111 0.0067 0.0033 0.003 0.0038 0.0081 – – – – – –
None 0.0058 0.0001 0.0011 0.0002 0.0001 0.0009 0.001 0.0001 0.0004 0.0012 0.0012 0.0001 – – – – – –
Nuclear Periphery 0.0339 0.0005 0.0032 0.0038 0.0002 0.0063 0.0128 0.0007 0.0022 0.0019 0.0164 0.0002 – – – – – –
Nucleolus 0.0006 0.0003 0.0002 0.0002 0 0.0007 0.0001 0 0.0001 0.0001 0.0006 0 – – – – – –
Nucleus 0.0101 0.0002 0.0007 0.0004 0.0001 0.0023 0.001 0.0001 0.0003 0.0008 0.0016 0.0001 – – – – – –
Peroxisomes 0.005 0.0013 0.0056 0.002 0.0018 0.0011 0.0049 0.0011 0.0016 0.0046 0.0314 0.0006 – – – – – –
Punctate Nuclear 0.0181 0.0002 0.0011 0.0008 0.0004 0.0008 0.0016 0.0001 0.0008 0.0015 0.0017 0.0001 – – – – – –
Vacuole 0.0039 0.0041 0.0174 0.0143 0.0013 0.0039 0.0022 0.0027 0.0062 0.0038 0.0046 0.0016 – – – – – –
Vacuole Periphery 0.0048 0.0037 0.0156 0.0259 0.0018 0.0048 0.0022 0.0022 0.0093 0.0048 0.0078 0.0023 – – – – – –

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 59.8465 43.3191 44.0382 54.4851 48.6923 51.8221 57.0494 53.8028 62.1102 61.0584
Translational Efficiency 1.7945 1.9787 1.5592 1.3062 1.5435 2.4248 1.6694 1.4271 1.3082 1.4575

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✔

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
983 1513 1748 325 403 1823 1517 1983 1386 3336 3265 2308

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1559.99 1243.80 1475.64 1634.47 1390.57 1378.46 1401.22 1261.33 1510.73 1317.39 1441.06 1313.87
Standard Deviation 334.85 296.19 233.72 295.43 252.39 273.92 256.33 153.62 322.44 292.04 247.29 222.29
Intensity Change Log 2 — -0.326782 -0.080196 0.067286 — -0.012619 0.011007 -0.140731 — -0.170178 -0.036494 -0.027027

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.025973 0.051845 0.013051 0.014532 0.066314 0.033437 0.048670 0.037337 0.037702 0.041786 0.029601 0.034126
Bud Neck 0.004236 0.002882 0.005688 0.006829 0.009898 0.001899 0.005457 0.005072 0.005882 0.002345 0.005580 0.005319
Bud Site 0.034395 0.019522 0.042893 0.042731 0.077689 0.012482 0.041922 0.065101 0.046983 0.015675 0.042442 0.061951
Cell Periphery 0.003972 0.002072 0.004196 0.004785 0.031328 0.002122 0.004504 0.007824 0.011926 0.002100 0.004339 0.007397
Cytoplasm 0.004447 0.005716 0.014776 0.005042 0.020127 0.002018 0.032148 0.036539 0.009006 0.003695 0.022847 0.032104
Cytoplasmic Foci 0.047427 0.097086 0.140036 0.077780 0.085905 0.072206 0.126370 0.179569 0.058615 0.083490 0.133686 0.165236
Eisosomes 0.000497 0.000775 0.000241 0.000279 0.002189 0.000916 0.002260 0.001844 0.000989 0.000852 0.001179 0.001623
Endoplasmic Reticulum 0.035006 0.031342 0.056820 0.084629 0.121371* 0.037314 0.045644 0.042460 0.060118 0.034606 0.051628 0.048398
Endosome 0.196302 0.208243* 0.227336* 0.212205* 0.199179 0.249324* 0.182911 0.230010* 0.197138 0.230693* 0.206695* 0.227503*
Golgi 0.436355* 0.415338* 0.259259* 0.275484* 0.297184* 0.441061* 0.236117* 0.207415* 0.395889* 0.429395* 0.248507* 0.217000*
Lipid Particles 0.088241 0.140185* 0.114038* 0.080803 0.042656 0.113835* 0.228358* 0.105980* 0.074986 0.125786* 0.167154* 0.102435*
Mitochondria 0.005100 0.005011 0.000972 0.000885 0.004172 0.007231 0.007593 0.006151 0.004830 0.006224 0.004048 0.005410
Mitotic Spindle 0.002233 0.000565 0.005038 0.001682 0.005379 0.001499 0.000524 0.000264 0.003148 0.001075 0.002941 0.000464
None 0.000092 0.000349 0.000048 0.000046 0.000054 0.000073 0.000131 0.000044 0.000081 0.000198 0.000086 0.000044
Nuclear Periphery 0.000498 0.000127 0.000406 0.001222 0.000123 0.000180 0.000074 0.000041 0.000389 0.000156 0.000252 0.000207
Nuclear Periphery Foci 0.003905 0.001333 0.007961 0.013352 0.001577 0.000727 0.001252 0.000675 0.003228 0.001002 0.004844 0.002460
Nucleolus 0.000025 0.000115 0.000019 0.000018 0.000014 0.000026 0.000024 0.000028 0.000022 0.000066 0.000021 0.000026
Nucleus 0.000079 0.000107 0.000143 0.000101 0.000208 0.000094 0.000260 0.000184 0.000117 0.000100 0.000197 0.000172
Peroxisomes 0.001599 0.002019 0.001094 0.000737 0.004954 0.001266 0.015527 0.016625 0.002575 0.001607 0.007800 0.014387
Vacuole 0.081293 0.008600 0.089157 0.155086 0.017600 0.010359 0.013252 0.038147 0.062774 0.009561 0.053889 0.054613
Vacuole Periphery 0.028325 0.006767 0.016827 0.021772 0.012082 0.011929 0.007004 0.018691 0.023602 0.009588 0.012263 0.019125

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.64 3.91 2.96 8.77 -0.50 4.62 2.35 4.04 -1.18 2.84 -1.14 2.39 1.01 2.65 -1.64
Bud Neck 2.54 -2.38 -2.33 -3.76 -0.98 6.88 3.49 4.08 -8.24 0.61 7.17 0.53 1.13 -8.15 0.69
Bud Site 3.81 -2.00 -1.18 -3.79 0.06 8.87 4.62 1.60 -16.56 -5.93 9.06 1.21 -3.46 -16.14 -6.06
Cell Periphery 2.95 -0.34 -0.53 -1.91 -0.39 6.85 6.29 5.48 -9.24 -5.37 7.24 5.55 3.17 -9.63 -5.36
Cytoplasm -0.76 -7.20 -0.35 0.32 6.23 6.26 -3.52 -4.92 -20.26 -1.62 4.87 -9.06 -12.57 -18.55 -4.93
Cytoplasmic Foci -11.75 -21.34 -4.88 3.06 9.49 2.31 -6.06 -14.21 -26.99 -10.76 -7.94 -21.16 -26.42 -22.27 -7.98
Eisosomes -3.49 4.26 3.22 7.61 -0.96 3.57 -0.17 0.95 -5.81 1.99 1.10 -1.39 -4.10 -6.22 -3.31
Endoplasmic Reticulum 1.11 -5.49 -5.00 -5.45 -2.78 8.68 7.74 8.12 -1.61 0.96 6.73 2.09 2.74 -4.86 1.03
Endosome -1.84 -4.82 -1.64 -0.39 1.66 -5.30 1.73 -3.32 3.23 -7.93 -6.32 -1.81 -5.39 0.67 -4.49
Golgi 1.76 17.15 10.39 9.80 -1.20 -10.09 4.37 6.65 32.03 4.18 -3.92 17.45 20.98 35.36 5.65
Lipid Particles -6.75 -3.82 0.76 5.91 3.57 -10.64 -22.45 -10.04 1.33 15.98 -9.43 -16.25 -5.07 4.69 12.24
Mitochondria 0.06 3.80 3.80 3.76 0.27 -1.85 -2.36 -1.47 0.85 1.46 -1.22 0.85 -0.59 0.87 -2.14
Mitotic Spindle 3.06 -3.35 0.87 -3.21 4.49 1.36 1.73 1.82 1.76 3.91 2.15 0.11 2.97 1.56 6.62
None -2.01 2.15 2.25 2.43 0.32 -1.49 -7.01 1.21 2.64 9.90 -1.95 -0.22 2.55 2.71 7.83
Nuclear Periphery 8.87 1.24 -1.66 -2.51 -1.84 -2.15 3.17 5.60 6.13 7.21 7.67 3.25 2.71 -0.81 0.70
Nuclear Periphery Foci 5.62 -5.08 -3.48 -4.48 -1.92 2.91 1.06 3.19 0.45 4.84 6.90 -3.38 1.56 -3.62 4.46
Nucleolus -1.27 0.71 0.94 1.38 0.52 -1.02 -2.63 -3.80 -0.11 -1.86 -1.35 0.09 -0.75 1.24 -2.34
Nucleus -1.26 -4.10 -1.56 0.25 2.92 2.04 -1.13 0.56 -2.50 3.94 0.71 -4.55 -3.42 -3.30 2.37
Peroxisomes -0.84 1.35 2.23 3.58 2.72 5.60 -6.66 -9.13 -13.62 -0.62 2.57 -6.86 -11.64 -13.04 -5.59
Vacuole 18.02 -1.56 -6.26 -13.23 -5.71 3.76 2.12 -8.21 -14.83 -12.72 17.66 2.50 2.20 -19.01 -0.16
Vacuole Periphery 11.05 5.50 2.26 -6.75 -2.13 0.07 2.62 -3.17 -5.62 -12.35 9.05 7.31 2.66 -9.97 -7.14
Standard name
Human Ortholog
Description AAA ATPase and SNARE disassembly chaperone; required for vesicular transport between ER and Golgi, the 'priming' step in homotypic vacuole fusion, autophagy, and protein secretion; releases Sec17p from SNAP complexes; has similarity to mammalian N-ethylmaleimide-sensitive factor (NSF)
Localization
Cell Percentages vacuole (3%), Golgi (2%), mixed (52%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Sec18

Sec18


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sec18-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available