Standard name
Human Ortholog
Description Transcription factor targeting filamentation genes and Ty1 expression; Ste12p activation of most filamentation gene promoters depends on Tec1p and Tec1p transcriptional activity is dependent on its association with Ste12p; binds to TCS elements upstream of filamentation genes, which are regulated by Tec1p/Ste12p/Dig1p complex; competes with Dig2p for binding to Ste12p/Dig1p; positive regulator of chronological life span; TEA/ATTS DNA-binding domain family member

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.14 0.27 0.25 0.29 0.32 0.18 0.21 0.13 0.24 0.14 0.1 0.36 0.66 0.9 0.52 0.48 0.52 0.09 0.18 0.13 0.82 0.91 0.81
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0.09 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0.07 0.12 0.16 0 0 0.05 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.67 0.57 0.52 0.57 0.63 0.82 0.75 0.8 0.77 0.8 0.83 0.13 0 0 0.22 0.07 0.07 0.07 0 0.09 0.09 0 0.05
Nucleus 0.34 0.37 0.21 0.19 0.27 0.28 0.27 0.31 0.35 0.34 0.42 0.33 0.11 0 0.15 0.13 0 0.4 0.43 0.31 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0.05 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.05 0.05 0 0 0
Vac/Vac Membrane 0.15 0.14 0.11 0.1 0.05 0 0 0 0 0.06 0 0.2 0.2 0.08 0.18 0.27 0.41 0.21 0.16 0.24 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 2
Bud 0 0 0 1 2 0 1 3 4 5 1 0 0 0 0 0 0 1 2 1 0 0 3
Bud Neck 0 0 0 0 0 0 2 0 0 0 0 0 0 0 1 0 0 2 1 0 0 0 1
Bud Site 0 0 1 1 0 1 3 4 7 1 10 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0
Cytoplasm 30 22 19 33 34 33 36 15 49 18 22 52 149 220 85 53 23 21 21 9 103 147 183
Endoplasmic Reticulum 0 0 1 1 0 0 0 1 2 0 0 0 2 0 9 6 4 0 0 0 0 0 0
Endosome 0 0 2 5 0 0 0 0 0 0 0 10 10 9 12 13 7 9 2 3 1 0 0
Golgi 0 0 1 0 0 0 0 0 0 0 0 0 0 2 2 0 0 2 0 0 0 0 0
Mitochondria 141 47 39 64 67 150 126 94 160 107 185 19 10 2 36 8 3 17 3 6 11 5 11
Nucleus 71 31 16 21 29 52 45 36 73 45 93 47 25 3 25 14 1 99 54 21 1 0 0
Nuclear Periphery 1 0 1 0 0 3 0 1 3 1 0 0 0 0 0 0 0 0 1 0 0 0 0
Nucleolus 1 0 0 1 2 2 1 2 3 7 6 1 0 2 1 0 0 6 5 1 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 2 0 1 2 0 5 7 0 1 0 4 1 0 3 1 4 1 18 6 3 1 0 3
Vac/Vac Membrane 32 12 8 11 5 7 4 5 3 8 4 29 45 19 29 30 18 52 20 16 2 1 7
Unique Cell Count 209 83 75 112 107 183 168 118 207 133 224 143 227 245 162 111 44 246 125 69 127 162 226
Labelled Cell Count 278 112 89 140 139 253 225 161 305 192 325 159 241 261 202 129 57 246 125 69 127 162 226


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.5 3.6 3.7 3.7 3.4 2.7 3.1 3.2 3.1 3.0 2.8 4.4 4.0 4.1 5.0 5.1 5.9 3.9 4.1 5.0
Std Deviation (1e-4) 0.5 0.6 1.4 1.6 1.5 0.9 1.1 1.3 2.0 1.0 1.1 0.9 1.0 1.5 2.2 1.5 2.4 1.2 1.7 2.4
Intensity Change (Log2)


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.6406 0.721 0.4804 0.0029 -0.2876 0.6516 0.5812 0.8117 0.7684 0.8204 0.2495 0.9099 1.8815 1.8516 1.3307 1.6422 1.2841 1.8451
Actin 0.0327 0.0005 0.0283 0.0006 0.0006 0.0037 0.0186 0.0017 0.0159 0.0049 0.0153 0.0037 0.0421 0.0002 0.0216 0.0055 0.0138 0.0043
Bud 0.0001 0.0012 0.007 0.0002 0.0007 0.0002 0.0005 0.001 0.0012 0.0003 0.0001 0.0003 0.0009 0.0002 0.0004 0.0001 0.0011 0.0002
Bud Neck 0.0015 0.0012 0.0015 0.0012 0.0062 0.0064 0.0022 0.0004 0.0103 0.0012 0.0011 0.0022 0.0037 0.0009 0.0026 0.0008 0.0021 0.005
Bud Periphery 0.0002 0.0012 0.0043 0.0003 0.0008 0.0003 0.0007 0.002 0.0022 0.0005 0.0001 0.0009 0.0016 0.0002 0.0006 0.0001 0.0061 0.0004
Bud Site 0.0006 0.0035 0.0036 0.0003 0.0007 0.0005 0.018 0.0026 0.0104 0.0008 0.0002 0.0003 0.0183 0.0018 0.0063 0.0005 0.002 0.0003
Cell Periphery 0.0001 0.0002 0.0003 0.0001 0.0001 0.0001 0.0005 0.0001 0.0005 0.0001 0 0.0001 0.0004 0.0002 0.0004 0.0001 0.0004 0.0001
Cytoplasm 0.086 0.1241 0.0905 0.0989 0.1038 0.0689 0.0698 0.1485 0.0976 0.0713 0.1705 0.0716 0.0939 0.0892 0.1015 0.0602 0.0629 0.0378
Cytoplasmic Foci 0.0086 0.006 0.0058 0.0058 0.0045 0.0018 0.0427 0.0115 0.0114 0.0472 0.0115 0.012 0.0205 0.0038 0.006 0.0055 0.006 0.0078
Eisosomes 0.0005 0.0001 0.0002 0.0001 0 0.0001 0.0007 0.0001 0.0004 0.0001 0.0001 0.0002 0.0004 0.0001 0.0002 0.0002 0.0004 0.0001
Endoplasmic Reticulum 0.0046 0.006 0.0051 0.0039 0.0034 0.0016 0.0023 0.0029 0.0055 0.0027 0.0023 0.002 0.0049 0.0033 0.0024 0.0015 0.0037 0.0011
Endosome 0.0075 0.003 0.0037 0.0043 0.0045 0.0047 0.0246 0.0037 0.0177 0.0245 0.0024 0.0079 0.0117 0.0027 0.003 0.0023 0.0175 0.0036
Golgi 0.0013 0.0002 0.0021 0.0001 0.0001 0.0008 0.0085 0.0023 0.0113 0.0204 0.001 0.0033 0.0079 0.0001 0.0011 0.004 0.0081 0.0016
Lipid Particles 0.009 0.0008 0.0031 0.0005 0.0003 0.0013 0.0218 0.0011 0.0041 0.068 0.0014 0.0114 0.0067 0.0003 0.001 0.0028 0.0098 0.0021
Mitochondria 0.0014 0.0007 0.0014 0.001 0.0009 0.0013 0.0102 0.0031 0.0129 0.0186 0.0011 0.006 0.0045 0.0006 0.0009 0.0182 0.0372 0.0015
None 0.1829 0.2305 0.1858 0.3544 0.0913 0.0867 0.3365 0.455 0.3841 0.4851 0.4338 0.288 0.1778 0.2529 0.2479 0.491 0.2381 0.1192
Nuclear Periphery 0.0162 0.013 0.019 0.019 0.0197 0.0109 0.0117 0.0072 0.0146 0.007 0.009 0.0089 0.016 0.0227 0.0142 0.0101 0.0384 0.0046
Nucleolus 0.0121 0.0122 0.0064 0.0046 0.0205 0.0205 0.006 0.0038 0.0081 0.006 0.0062 0.0189 0.0096 0.0089 0.0101 0.0059 0.0149 0.0263
Nucleus 0.5769 0.551 0.5801 0.4023 0.6657 0.7543 0.3408 0.2999 0.3487 0.1105 0.2637 0.5087 0.5136 0.5477 0.5146 0.3412 0.4345 0.7114
Peroxisomes 0.0028 0.0002 0.0145 0.0002 0.0003 0.0008 0.0157 0.0212 0.0026 0.0807 0.0336 0.0147 0.01 0.0001 0.004 0.0148 0.0041 0.0022
Punctate Nuclear 0.0522 0.0411 0.0343 0.0997 0.0714 0.0339 0.0631 0.0295 0.0333 0.0471 0.0455 0.0368 0.0526 0.0613 0.0595 0.034 0.0938 0.0692
Vacuole 0.0022 0.0031 0.0026 0.0021 0.0038 0.001 0.0041 0.0019 0.006 0.0022 0.0008 0.0013 0.002 0.0024 0.0013 0.0007 0.0022 0.0011
Vacuole Periphery 0.0007 0.0005 0.0006 0.0006 0.0006 0.0003 0.001 0.0003 0.0011 0.0007 0.0002 0.0005 0.0008 0.0005 0.0003 0.0003 0.0027 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 6.4195 7.4265 7.5147 34.4007 17.136 4.5289 7.3383 6.6806 16.8348 16.7435
Translational Efficiency 0.4036 0.3404 1.2696 0.7407 1.1137 0.7111 0.4723 0.8898 1.0543 0.8884

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency