Standard name
Description Mitochondrial C1-tetrahydrofolate synthase; involved in interconversion between different oxidation states of tetrahydrofolate (THF); provides activities of formyl-THF synthetase, methenyl-THF cyclohydrolase, and methylene-THF dehydrogenase

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0.06
Bud 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.05 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0.08 0 0 0 0 0 0
Golgi 0.1 0 0 0 0 0 0 0 0 0 0 0 0 0.13 0 0.29 0.42 0.4 0 0.07 0 0.12 0.1 0.1
Mitochondria 0.87 0.95 0.92 0.93 0.9 0.9 0.89 0.91 0.97 0.97 0.95 0.96 0.94 0.91 0.95 0.06 0.12 0.08 0.88 0.74 0.82 0.65 0.66 0.66
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.05 0.06 0 0 0.09 0.09 0.11 0.07 0.08 0.08 0.09 0.11 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.07 0 0 0 0 0.07 0.06 0.08 0.06 0 0.06 0 0 0 0 0.08 0 0.09 0 0 0 0 0 0
Peroxisomes 0.09 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0.21 0.13 0.09 0.05 0 0.06 0.06 0.07 0.12
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0 0 0 0 0 0 0
Vac/Vac Membrane 0.12 0.12 0.1 0 0.06 0.08 0.07 0 0 0 0 0 0.08 0.09 0.06 0.19 0.19 0.13 0 0.05 0 0 0.06 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 1 5 1 1 2 2 0 0 0 1 0 4 2 2 3 2 1 4 3 10 14 22
Bud 3 4 8 9 8 4 24 24 12 21 23 19 2 5 12 2 8 6 1 2 1 1 1 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Bud Site 0 1 2 0 1 1 5 7 3 4 6 4 0 0 0 0 0 1
Cell Periphery 5 4 8 11 4 4 3 3 5 6 0 7 5 11 10 8 11 9 0 0 0 0 0 1
Cytoplasm 0 1 6 7 14 11 10 12 6 2 10 6 6 10 8 6 18 8 0 0 1 0 0 0
Endoplasmic Reticulum 0 0 1 3 0 4 2 3 4 4 4 4 1 5 5 2 5 7 0 0 0 1 0 0
Endosome 5 2 12 1 10 3 11 13 0 1 5 0 3 8 2 14 8 13 3 7 17 8 10 8
Golgi 23 0 4 3 4 3 0 0 0 0 0 0 8 55 11 55 80 64 7 11 10 25 32 36
Mitochondria 196 154 345 290 308 395 608 761 370 405 430 493 300 386 437 12 24 13 224 123 317 136 202 251
Nucleus 1 0 0 0 1 1 6 7 0 2 2 2 0 1 0 0 3 0 0 0 1 0 0 0
Nuclear Periphery 12 10 13 8 32 39 72 57 32 32 43 57 14 12 12 8 4 6 1 1 2 3 1 2
Nucleolus 15 3 11 11 15 32 42 65 23 18 26 20 7 3 9 15 7 15 1 0 0 0 3 1
Peroxisomes 21 3 23 7 15 1 28 13 0 0 3 5 10 9 10 39 25 15 12 5 21 13 21 44
SpindlePole 3 1 5 3 5 6 9 20 3 3 8 7 2 1 3 11 12 5 0 0 1 0 1 1
Vac/Vac Membrane 26 19 36 12 19 37 49 36 12 15 14 21 26 38 27 35 37 21 0 8 3 9 17 6
Unique Cell Count 226 162 373 311 344 439 682 833 382 416 455 513 320 425 460 188 192 160 255 167 388 211 309 382
Labelled Cell Count 310 203 475 370 437 542 871 1023 470 513 574 646 384 548 548 209 245 185 255 167 388 211 309 382


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 12.6 9.3 7.2 9.6 8.9 7.6 6.7 6.2 5.7 5.4 5.3 4.9 11.0 10.3 8.2 11.1 12.9 13.6 12.2 13.1 13.5
Std Deviation (1e-4) 3.0 2.5 2.6 4.1 2.2 1.9 1.7 1.4 1.7 1.2 1.3 1.2 2.3 2.1 1.8 3.0 3.9 4.1 3.1 3.3 3.2
Intensity Change (Log2) 0.41 0.29 0.07 -0.11 -0.22 -0.35 -0.41 -0.44 -0.55 0.6 0.51 0.18 0.62 0.83 0.91 0.75 0.86 0.9


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0.6 0.2 -1.5 1.2 0.7 0.9 2.2 2.2 1.3 0 -1.1 0.4 0 1.4 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1.1 -1.0 -1.5 0 0 -0.9 -0.7 0 -0.9 -0.6 0.4 0 1.4 2.2 2.1
Cytoplasm 0.6 2.0 0.9 -0.2 -0.2 0 0 0.6 -0.6 0.3 0.7 0.1 0 4.3 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome -2.8 -0.2 -2.7 -1.7 -1.9 -3.5 -3.3 -2.1 -4.1 -2.1 -1.2 -3.1 2.2 0.6 2.5
Golgi 0 0 0 0 0 0 0 0 0 1.4 6.4 1.4 10.3 12.8 12.3
Mitochondria 0.4 -1.4 -1.3 -1.8 -0.7 2.7 3.1 1.2 2.3 0.6 -0.8 1.5 -20.0 -18.9 -19.0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery -0.7 3.2 3.1 4.0 2.3 2.8 2.5 3.4 4.2 0.6 -0.5 -0.7 0.5 -0.9 0.2
Nucleolus 0.4 1.0 2.8 2.3 3.2 2.0 1.0 1.9 0.8 -0.6 -2.4 -0.9 2.7 0.4 3.2
Peroxisomes -2.5 -1.1 -5.0 -1.5 -4.3 -4.9 -5.1 -4.5 -4.4 -1.9 -2.9 -2.9 5.2 2.8 1.3
SpindlePole 0 0 0 0 1.2 0 0 0.5 0 0 0 0 3.0 3.2 0
Vacuole -3.0 -2.1 -0.6 -1.4 -3.6 -3.7 -3.4 -4.0 -3.3 -0.7 -0.3 -2.1 3.0 3.2 1.2


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mis1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available