Standard name
Description Constitutively expressed acid phosphatase similar to Pho5p; brought to the cell surface by transport vesicles; hydrolyzes thiamin phosphates in the periplasmic space, increasing cellular thiamin uptake; expression is repressed by thiamin

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.97 0.93 0.96 0.93 0.79 0.71 0.78 0.69 0.68 0.46 0.51 1.0 0.99 0.99 0.9 0.86 0.9 0.72 0.77 0.64 0.76 0.73 0.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.24 0 0.06 0.09 0.08 0.37 0.4 0.34 0.5 0.55 0.69 0.66 0 0 0 0 0 0 0.13 0.11 0.22 0.06 0.05 0.06
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.13 0.27 0.14 0 0 0 0.08 0.09 0.08
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 3 3 3 6 8
Bud 0 0 5 1 6 2 6 15 10 10 10 11 0 1 0 0 0 0 0 0 2 7 16 16
Bud Neck 0 0 1 1 1 1 1 10 5 2 0 2 0 0 0 1 0 1 0 0 0 2 2 7
Bud Site 0 0 0 0 0 1 2 0 2 2 1 1 0 0 0 0 0 0
Cell Periphery 0 1 0 1 1 2 2 0 0 0 0 1 1 2 0 0 1 1 0 0 1 2 4 5
Cytoplasm 45 194 169 190 246 271 285 421 217 237 121 180 387 820 765 409 228 237 67 154 117 222 419 321
Endoplasmic Reticulum 0 2 0 2 0 0 0 1 1 1 3 0 0 2 2 37 12 10 3 1 2 2 5 1
Endosome 0 0 0 0 1 0 0 2 0 0 0 0 0 0 1 8 3 3 1 3 1 0 6 3
Golgi 0 0 2 1 1 0 0 1 0 0 0 0 0 1 0 1 1 1 0 1 1 0 4 3
Mitochondria 11 5 11 17 21 129 159 185 156 191 179 234 0 1 0 12 0 0 12 22 39 18 31 28
Nucleus 0 1 0 1 0 1 1 2 2 2 1 0 1 0 1 0 0 2 0 0 0 0 0 0
Nuclear Periphery 0 1 0 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 1
Nucleolus 0 0 1 0 1 2 1 3 0 0 0 0 0 0 0 0 2 0 0 0 0 0 1 0
Peroxisomes 0 0 0 0 0 1 1 0 0 0 0 0 0 2 1 0 0 0 0 0 1 0 0 2
SpindlePole 0 0 1 0 1 5 13 21 9 5 5 7 0 0 0 0 0 0 1 1 2 3 10 9
Vac/Vac Membrane 0 1 2 0 3 1 4 2 1 2 2 5 0 1 1 61 71 36 2 4 3 24 53 37
Unique Cell Count 46 201 182 198 264 345 400 541 315 348 261 353 388 828 770 452 266 263 94 199 183 294 574 460
Labelled Cell Count 56 205 193 215 283 416 475 663 403 454 322 441 389 830 771 529 318 291 94 199 183 294 574 460


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.2 3.8 3.5 3.4 3.7 2.9 2.6 2.9 2.7 2.6 2.6 2.4 3.9 4.0 4.1 4.9 5.9 5.6 3.8 4.3 4.8
Std Deviation (1e-4) 0.5 1.0 1.4 1.4 2.1 1.1 1.1 1.0 1.1 1.6 0.9 1.1 1.3 1.4 1.5 1.2 1.5 1.2 1.1 1.9 2.0
Intensity Change (Log2) -0.03 0.08 -0.28 -0.41 -0.27 -0.36 -0.45 -0.45 -0.53 0.16 0.18 0.22 0.5 0.75 0.69 0.13 0.29 0.46


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0 0
Bud Neck 0 0 0
Bud Site 0 0 0
Cell Periphery 0 0 0
Cyto
Endoplasmic Reticulum 0 0 0
Endosome 0 0 0
Golgi 0 0 0
Mitochondria 0 0 0
Nuclear Periphery 0 0 0
Nuc
Nucleolus 0 0 0
Peroxisomes 0 0 0
SpindlePole 0 0 0
Vac
Cortical Patches 0 0 0
Cytoplasm 0 0 0
Nucleus 0 0 0
Vacuole 4.7 7.2 4.7


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pho3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available