Standard name | |
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Description | Repressible acid phosphatase; 1 of 3 repressible acid phosphatases that also mediates extracellular nucleotide-derived phosphate hydrolysis; secretory pathway derived cell surface glycoprotein; induced by phosphate starvation and coordinately regulated by PHO4 and PHO2 |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 3 | 0 | 2 | 4 | 5 |
Bud | 0 | 1 | – | 0 | 2 | 2 | 0 | 2 | 1 | 0 | 2 | 1 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 3 | 3 | 14 |
Bud Neck | 0 | 1 | – | 0 | 3 | 1 | 2 | 6 | 1 | 2 | 0 | 1 | 0 | 0 | 0 | – | 1 | 0 | 0 | 2 | 0 | 3 | 0 | 4 |
Bud Site | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | – | 2 | 0 | 0 | 0 | 0 | 2 | 2 | 0 | 1 | 1 | 2 | 2 | – | 3 | 1 | 0 | 0 | 0 | 2 | 2 | 3 |
Cytoplasm | 22 | 140 | – | 83 | 116 | 116 | 130 | 125 | 70 | 94 | 64 | 50 | 426 | 472 | 524 | – | 120 | 71 | 51 | 119 | 30 | 210 | 205 | 247 |
Endoplasmic Reticulum | 0 | 1 | – | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 2 | 1 | – | 8 | 3 | 3 | 0 | 0 | 2 | 3 | 3 |
Endosome | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 2 | 0 | 0 | 4 | 1 | 1 | 3 | 3 |
Golgi | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 4 | 13 | – | 9 | 11 | 11 | 42 | 27 | 36 | 39 | 35 | 42 | 0 | 2 | 0 | – | 2 | 0 | 3 | 10 | 8 | 14 | 11 | 20 |
Nucleus | 1 | 1 | – | 1 | 1 | 1 | 2 | 3 | 1 | 1 | 0 | 2 | 0 | 3 | 2 | – | 7 | 3 | 0 | 0 | 0 | 0 | 1 | 0 |
Nuclear Periphery | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | – | 1 | 1 | 1 | 3 | 2 | 1 | 0 | 2 | 1 | 0 | 1 | 1 | – | 2 | 0 | 1 | 0 | 0 | 3 | 3 | 4 |
Vac/Vac Membrane | 0 | 1 | – | 0 | 0 | 0 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | – | 19 | 6 | 3 | 6 | 0 | 16 | 12 | 14 |
Unique Cell Count | 25 | 151 | 86 | 120 | 120 | 147 | 145 | 91 | 112 | 86 | 76 | 426 | 479 | 530 | 144 | 78 | 69 | 150 | 46 | 272 | 263 | 329 | ||
Labelled Cell Count | 29 | 160 | 96 | 134 | 132 | 181 | 167 | 113 | 140 | 103 | 99 | 427 | 484 | 532 | 165 | 84 | 69 | 150 | 46 | 272 | 263 | 329 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
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Mean Cell GFP Intensity (1e-4) | 3.6 | 3.7 | – | 3.1 | 3.1 | 3.1 | 2.4 | 3.1 | 2.6 | 2.3 | 2.4 | 2.4 | 4.1 | 4.0 | 4.1 | 4.6 | 5.6 | 5.4 | 3.7 | 3.9 | 4.5 |
Std Deviation (1e-4) | 1.7 | 0.9 | – | 1.5 | 1.2 | 1.2 | 1.0 | 1.7 | 1.0 | 1.0 | 1.1 | 1.0 | 1.2 | 1.2 | 1.4 | 1.1 | 1.3 | 1.5 | 1.4 | 1.6 | 1.5 |
Intensity Change (Log2) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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