Standard name
Human Ortholog
Description Repressible acid phosphatase; 1 of 3 repressible acid phosphatases that also mediates extracellular nucleotide-derived phosphate hydrolysis; secretory pathway derived cell surface glycoprotein; induced by phosphate starvation and coordinately regulated by PHO4 and PHO2

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.88 0.93 0.97 0.97 0.97 0.88 0.86 0.77 0.84 0.74 0.66 1.0 0.99 0.99 0.83 0.91 0.75 0.8 0.67 0.78 0.78 0.75
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0.05 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.16 0.09 0.1 0.09 0.09 0.29 0.19 0.4 0.35 0.41 0.55 0 0 0 0 0 0.05 0.07 0.18 0.06 0.05 0.06
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.13 0.08 0.05 0 0 0.06 0.05 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 2 4 5
Bud 0 1 0 2 2 0 2 1 0 2 1 0 1 0 0 0 0 0 0 3 3 14
Bud Neck 0 1 0 3 1 2 6 1 2 0 1 0 0 0 1 0 0 2 0 3 0 4
Bud Site 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Cell Periphery 0 1 2 0 0 0 0 2 2 0 1 1 2 2 3 1 0 0 0 2 2 3
Cytoplasm 22 140 83 116 116 130 125 70 94 64 50 426 472 524 120 71 51 119 30 210 205 247
Endoplasmic Reticulum 0 1 0 0 0 0 1 1 0 0 1 0 2 1 8 3 3 0 0 2 3 3
Endosome 1 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 4 1 1 3 3
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 0 0 0 0 0 0 0
Mitochondria 4 13 9 11 11 42 27 36 39 35 42 0 2 0 2 0 3 10 8 14 11 20
Nucleus 1 1 1 1 1 2 3 1 1 0 2 0 3 2 7 3 0 0 0 0 1 0
Nuclear Periphery 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 1 1 1 3 2 1 0 2 1 0 1 1 2 0 1 0 0 3 3 4
Vac/Vac Membrane 0 1 0 0 0 2 0 0 1 0 0 0 0 1 19 6 3 6 0 16 12 14
Unique Cell Count 25 151 86 120 120 147 145 91 112 86 76 426 479 530 144 78 69 150 46 272 263 329
Labelled Cell Count 29 160 96 134 132 181 167 113 140 103 99 427 484 532 165 84 69 150 46 272 263 329


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.6 3.7 3.1 3.1 3.1 2.4 3.1 2.6 2.3 2.4 2.4 4.1 4.0 4.1 4.6 5.6 5.4 3.7 3.9 4.5
Std Deviation (1e-4) 1.7 0.9 1.5 1.2 1.2 1.0 1.7 1.0 1.0 1.1 1.0 1.2 1.2 1.4 1.1 1.3 1.5 1.4 1.6 1.5
Intensity Change (Log2)


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.2896 1.7733 2.0344 2.1579 2.6299 1.8766 1.0047 0.9464 1.028 1.4442 0.8941 1.073 1.8887 1.2368 1.1469 0.7618 2.0872 1.1507
Actin 0.0248 0.0002 0.0099 0.0042 0.0156 0.0234 0.0122 0.0003 0.0024 0.0117 0.0165 0.0011 0.0727 0.011 0.0268 0.001 0.0033 0.0025
Bud 0.0004 0.0009 0.002 0.007 0.0003 0.0004 0.0002 0.0132 0.0002 0.0002 0.0015 0.0001 0.0025 0.0037 0.0062 0.001 0.0003 0.0118
Bud Neck 0.0013 0.0001 0.0001 0.0005 0.0008 0.0006 0.0016 0.0001 0 0.0002 0.0002 0.0003 0.0068 0.0008 0.0009 0.0003 0 0.0003
Bud Periphery 0.0006 0.001 0.0099 0.0068 0.0003 0.0009 0.0002 0.0032 0.0004 0.0002 0.006 0.0001 0.0182 0.0338 0.0059 0.0014 0.0041 0.0039
Bud Site 0.0023 0.0077 0.0019 0.0118 0.0003 0.0005 0.0032 0.0024 0.0001 0.0035 0.0032 0.0001 0.0295 0.0049 0.0018 0.001 0 0.0003
Cell Periphery 0.0002 0.0001 0.0003 0.0004 0.0001 0.0001 0.0002 0.0001 0.0001 0 0.0009 0 0.0113 0.0015 0.0002 0.0001 0.0003 0.0001
Cytoplasm 0.0077 0.0206 0.0134 0.0159 0.0564 0.0327 0.0086 0.0091 0.0047 0.0047 0.0105 0.0087 0.0113 0.0167 0.0112 0.0229 0.0073 0.0268
Cytoplasmic Foci 0.0219 0.0073 0.0071 0.0092 0.0069 0.0238 0.0792 0.0006 0.0017 0.0378 0.0043 0.0152 0.0184 0.005 0.0051 0.0073 0.0007 0.0039
Eisosomes 0.0003 0.0002 0.0004 0.0001 0.0001 0.0002 0.0009 0.0006 0.0004 0.0002 0.0017 0.0001 0.0067 0.0008 0.0005 0.0001 0.0013 0.0001
Endoplasmic Reticulum 0.0022 0.0006 0.0024 0.0011 0.0004 0.0004 0.036 0.0003 0.003 0.0008 0.0038 0.0001 0.0077 0.0021 0.001 0.0005 0.0005 0.0002
Endosome 0.0122 0.0031 0.0137 0.0287 0.0125 0.0028 0.0279 0 0.0087 0.0708 0.0115 0.0081 0.0255 0.0044 0.0011 0.0003 0.0001 0.0008
Golgi 0.004 0.0004 0.0034 0.0115 0.0415 0.0036 0.0043 0 0.0009 0.0207 0.0069 0.0106 0.013 0.0013 0.0013 0.0001 0.0001 0.0006
Lipid Particles 0.0125 0.0013 0.0017 0.0012 0.0013 0.0062 0.0087 0.0001 0.0011 0.0095 0.0027 0.0003 0.0278 0.0012 0.0009 0.0003 0.0002 0.002
Mitochondria 0.002 0.0002 0.0328 0.0082 0.01 0.0044 0.0071 0.0004 0.0011 0.0015 0.0603 0.0023 0.0122 0.0017 0.0032 0.0003 0.0007 0.0004
None 0.8554 0.9508 0.8924 0.8798 0.8516 0.8837 0.7194 0.9363 0.965 0.7947 0.8622 0.9504 0.7032 0.9029 0.9255 0.9593 0.9804 0.9385
Nuclear Periphery 0.0066 0.0003 0.004 0.0008 0.0001 0.0007 0.0417 0.0015 0.006 0.0006 0.0014 0 0.0136 0.0018 0.0015 0.0003 0.0001 0.0003
Nucleolus 0.0014 0.0007 0.0001 0.0002 0 0.0003 0.0002 0.0016 0.0004 0.0001 0.0003 0 0.0007 0.0007 0.0008 0.0002 0.0001 0.0002
Nucleus 0.0019 0.0007 0.0003 0.0009 0.0001 0.0002 0.0035 0.026 0.0009 0.0001 0.0008 0 0.0026 0.0014 0.0017 0.0005 0.0001 0.0012
Peroxisomes 0.0119 0.0004 0.001 0.001 0.0008 0.0129 0.0374 0 0.0004 0.0386 0.0018 0.0009 0.0082 0.0006 0.0026 0.0003 0.0001 0.004
Punctate Nuclear 0.0262 0.003 0.0006 0.0004 0.0001 0.0016 0.0034 0.0037 0.001 0.0019 0.0002 0.0012 0.0042 0.0025 0.0013 0.0027 0.0001 0.0015
Vacuole 0.0028 0.0004 0.0012 0.0082 0.0004 0.0003 0.0017 0.0003 0.0008 0.0012 0.0019 0.0001 0.0023 0.0011 0.0004 0.0002 0.0001 0.0005
Vacuole Periphery 0.0014 0.0001 0.0014 0.002 0.0003 0.0002 0.0024 0.0001 0.0008 0.0009 0.0012 0.0001 0.0015 0.0003 0.0002 0 0 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 45.9752 42.8554 244.4992 168.643 49.9288 57.339 83.1103 175.2334 230.7407 104.1635
Translational Efficiency 0.4517 0.4944 0.7119 0.8125 0.7694 0.5313 0.7199 0.8054 0.9394 0.8264

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency