Standard name
Description Repressible acid phosphatase; 1 of 3 repressible acid phosphatases that also mediates extracellular nucleotide-derived phosphate hydrolysis; secretory pathway derived cell surface glycoprotein; induced by phosphate starvation and coordinately regulated by PHO4 and PHO2

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.88 0.93 0.97 0.97 0.97 0.88 0.86 0.77 0.84 0.74 0.66 1.0 0.99 0.99 0.83 0.91 0.75 0.8 0.67 0.78 0.78 0.75
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0.05 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.16 0.09 0.1 0.09 0.09 0.29 0.19 0.4 0.35 0.41 0.55 0 0 0 0 0 0.05 0.07 0.18 0.06 0.05 0.06
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.13 0.08 0.05 0 0 0.06 0.05 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 2 4 5
Bud 0 1 0 2 2 0 2 1 0 2 1 0 1 0 0 0 0 0 0 3 3 14
Bud Neck 0 1 0 3 1 2 6 1 2 0 1 0 0 0 1 0 0 2 0 3 0 4
Bud Site 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Cell Periphery 0 1 2 0 0 0 0 2 2 0 1 1 2 2 3 1 0 0 0 2 2 3
Cytoplasm 22 140 83 116 116 130 125 70 94 64 50 426 472 524 120 71 51 119 30 210 205 247
Endoplasmic Reticulum 0 1 0 0 0 0 1 1 0 0 1 0 2 1 8 3 3 0 0 2 3 3
Endosome 1 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 4 1 1 3 3
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 0 0 0 0 0 0 0
Mitochondria 4 13 9 11 11 42 27 36 39 35 42 0 2 0 2 0 3 10 8 14 11 20
Nucleus 1 1 1 1 1 2 3 1 1 0 2 0 3 2 7 3 0 0 0 0 1 0
Nuclear Periphery 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 1 1 1 3 2 1 0 2 1 0 1 1 2 0 1 0 0 3 3 4
Vac/Vac Membrane 0 1 0 0 0 2 0 0 1 0 0 0 0 1 19 6 3 6 0 16 12 14
Unique Cell Count 25 151 86 120 120 147 145 91 112 86 76 426 479 530 144 78 69 150 46 272 263 329
Labelled Cell Count 29 160 96 134 132 181 167 113 140 103 99 427 484 532 165 84 69 150 46 272 263 329


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.6 3.7 3.1 3.1 3.1 2.4 3.1 2.6 2.3 2.4 2.4 4.1 4.0 4.1 4.6 5.6 5.4 3.7 3.9 4.5
Std Deviation (1e-4) 1.7 0.9 1.5 1.2 1.2 1.0 1.7 1.0 1.0 1.1 1.0 1.2 1.2 1.4 1.1 1.3 1.5 1.4 1.6 1.5
Intensity Change (Log2)


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pho5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available