Standard name
Human Ortholog
Description Serine/threonine protein kinase involved in vacuolar protein sorting; functions as a membrane-associated complex with Vps34p; active form recruits Vps34p to the Golgi membrane; interacts with the GDP-bound form of Gpa1p; myristoylated; a fraction is localized, with Vps34p, to nuclear pores at nucleus-vacuole junctions and may facilitate transcription elongation for genes positioned at the nuclear periphery

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0.06 0.08 0.08 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.14 0.2 0.16 0.14 0.12 0.16 0.25 0.22 0.22 0.31 0.27 0.34 0.28 0.4 0.41 0.44 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0.09 0.29 0.05 0 0 0 0 0 0 0 0
Endosome 0.7 0.54 0.66 0.49 0.59 0.1 0.12 0.11 0 0 0 0.35 0.17 0.31 0.35 0.24 0.79 0.69 0.76 0.68 0.67 0.63
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.15 0.11 0.26 0.29 0.74 0.61 0.64 0.81 0.75 0.79 0 0 0 0.08 0.07 0 0 0.07 0.06 0.08 0.07
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0.11 0.07 0.1 0 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.14 0.07 0.28 0.25 0.19 0.12 0.12 0.16 0.11 0.07 0.06 0.16 0.11 0.09 0.23 0.1 0 0 0 0 0 0
Vac/Vac Membrane 0.31 0.3 0.09 0 0.08 0 0 0 0 0 0 0.23 0.21 0.26 0.17 0.27 0.1 0.13 0.06 0.15 0.16 0.2
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 3 1 0 0 0 1 0 0 0 0 0 5 2 1 0 1 1 9 2 1 1 2
Bud 3 7 7 5 16 18 24 53 48 32 26 2 0 0 0 1 4 2 0 1 0 1
Bud Neck 0 0 0 0 0 1 0 0 0 0 0 1 0 2 0 1 1 2 0 0 0 2
Bud Site 1 0 1 0 0 2 1 2 4 3 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 1 0 1 0 1 0 1 0 0 0 0 0 0 0 0 0
Cytoplasm 85 84 60 31 44 75 103 140 137 142 156 66 32 64 29 18 15 18 8 13 7 7
Endoplasmic Reticulum 5 7 0 1 2 6 1 11 0 2 7 18 33 8 3 0 0 1 1 0 3 5
Endosome 433 221 252 107 215 47 50 69 13 10 13 68 19 50 25 10 540 343 323 240 224 237
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 6 3 5 3 3 3
Mitochondria 26 64 42 57 106 336 247 408 498 344 466 0 0 0 6 3 20 21 29 21 28 25
Nucleus 3 0 0 0 0 0 2 1 0 1 2 0 2 1 2 0 1 1 0 0 0 0
Nuclear Periphery 0 0 0 2 1 1 4 2 0 0 1 1 0 0 0 0 3 2 3 2 0 4
Nucleolus 6 4 2 5 3 5 2 13 2 3 2 0 0 0 1 0 1 1 1 1 0 0
Peroxisomes 14 3 43 15 36 15 20 33 10 7 5 0 0 0 0 0 1 1 2 1 1 0
SpindlePole 87 29 108 55 71 53 50 100 68 30 36 31 12 14 16 4 10 12 16 6 5 6
Vac/Vac Membrane 191 122 36 9 28 14 18 12 17 16 21 45 24 42 12 11 65 66 26 54 54 74
Unique Cell Count 622 413 382 219 365 456 404 639 617 459 587 197 114 159 71 41 681 497 427 352 335 377
Labelled Cell Count 858 542 551 287 522 574 523 844 798 590 736 237 125 182 95 49 681 497 427 352 335 377


Endosome

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.4 5.4 4.5 4.2 4.2 3.4 3.3 3.6 3.1 3.3 3.2 6.0 6.2 6.2 5.3 6.1 6.0 6.6 6.8
Std Deviation (1e-4) 0.7 1.0 1.2 0.9 1.4 1.1 1.1 0.9 0.6 0.9 0.6 0.8 0.8 0.8 1.2 2.0 1.2 1.3 1.7
Intensity Change (Log2) -0.1 -0.1 -0.38 -0.45 -0.3 -0.52 -0.43 -0.48 0.42 0.48 0.47 0.25 0.44 0.43 0.56 0.6


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.5 -1.4 0.3 3.4 2.4 2.5 5.1 4.0 4.9 3.0 6.2 4.9 4.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 6.0 10.9 0 0 0
Endosome 0 0 0 0 0 0 0 0 -7.2 -9.3 -7.4 -4.9 -5.2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 -4.8 -3.7 -4.4 -0.6 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 -4.9 -3.7 -4.4 -3.0 0
SpindlePole 0 0 0 0 0 0 0 0 -3.3 -3.9 -4.9 -1.0 -2.6
Vacuole -2.4 -0.9 -3.9 -2.8 -5.5 -4.6 -3.6 -3.8 4.4 3.3 5.1 1.9 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration
Actin 0.017 0.0002 0.0007 0.0022 0.0062 0.0001 0.0097 0.0009 0.0285 0.0001 0 0.0009 0 0.0006 0.0281 0 0 0.0002
Bud 0.0004 0.0004 0.0005 0.0007 0.0002 0.0001 0.0008 0.0011 0.0082 0.0003 0 0.0001 0 0.0002 0.0001 0 0 0.0001
Bud Neck 0.0041 0.0007 0.0038 0.0003 0.0006 0.0004 0.0063 0.0008 0.0011 0.0001 0.0002 0.0005 0 0.0005 0.0003 0.0034 0.0002 0.0007
Bud Periphery 0.0007 0.0003 0.0006 0.0008 0.0003 0.0001 0.0017 0.0021 0.0076 0.0004 0 0.0002 0 0.0002 0.0002 0.0001 0 0
Bud Site 0.003 0.0049 0.0036 0.0018 0.0009 0.0002 0.0386 0.0029 0.0039 0.0003 0.0002 0.0003 0 0.0021 0.0006 0.001 0.0001 0.0003
Cell Periphery 0.0003 0.0002 0.0002 0.0002 0.0002 0.0001 0.0018 0.0004 0.0005 0.0002 0 0.0001 0 0.0004 0.0001 0.0004 0 0.0001
Cytoplasm 0.0255 0.107 0.0756 0.0907 0.0239 0.0387 0.0272 0.0559 0.0272 0.0457 0.0168 0.0222 0.0005 0.1029 0.03 0.0077 0.0035 0.0243
Cytoplasmic Foci 0.1377 0.1541 0.1636 0.1358 0.1417 0.1726 0.1721 0.1384 0.2002 0.1568 0.3178 0.1961 0.3261 0.1618 0.2493 0.1118 0.2483 0.2194
Eisosomes 0.0003 0.0001 0.0001 0 0.0001 0 0.001 0.0002 0.0003 0 0 0.0001 0 0.0003 0.0001 0 0 0
Endoplasmic Reticulum 0.0039 0.004 0.003 0.0042 0.001 0.0013 0.0045 0.0029 0.002 0.0055 0.0003 0.0014 0.0001 0.0111 0.0016 0.0016 0.0004 0.0051
Endosome 0.5617 0.5014 0.4873 0.5787 0.6689 0.5776 0.5408 0.4881 0.4806 0.6222 0.6212 0.5623 0.1048 0.4093 0.4459 0.7371 0.596 0.5417
Golgi 0.0173 0.0117 0.021 0.0234 0.0239 0.0237 0.02 0.0157 0.0304 0.0218 0.0211 0.0298 0.013 0.0242 0.0209 0.0171 0.0324 0.0322
Lipid Particles 0.0815 0.0242 0.0469 0.0112 0.025 0.0746 0.0729 0.0458 0.0736 0.0149 0.0071 0.0735 0.4258 0.0711 0.0589 0.0615 0.0879 0.1085
Mitochondria 0.0058 0.0018 0.011 0.0164 0.0328 0.0027 0.0196 0.0206 0.0161 0.0079 0.0007 0.007 0.0004 0.0062 0.0067 0.0009 0.002 0.0017
None 0.0369 0.0957 0.1024 0.0271 0.0042 0.0526 0.0124 0.1507 0.0816 0.0677 0.0009 0.0578 0.0003 0.1394 0.0833 0.0081 0.0019 0.0033
Nuclear Periphery 0.0137 0.0038 0.003 0.0055 0.0005 0.0012 0.0065 0.0012 0.0022 0.0028 0.0001 0.0011 0.0001 0.0049 0.0015 0.0012 0.0005 0.0021
Nucleolus 0.0013 0.0014 0.0016 0.0004 0.0007 0.0007 0.0023 0.0009 0.0007 0.0002 0.0001 0.0003 0.0001 0.0037 0.0004 0.0103 0.0001 0.0007
Nucleus 0.0024 0.0028 0.0014 0.0013 0.0005 0.001 0.0051 0.0011 0.0018 0.0009 0.0001 0.0005 0 0.0056 0.0004 0.0031 0.0001 0.0006
Peroxisomes 0.0116 0.0035 0.0185 0.02 0.0297 0.0222 0.0141 0.009 0.0159 0.0062 0.0062 0.0204 0.1266 0.0078 0.0416 0.0029 0.0173 0.0195
Punctate Nuclear 0.022 0.0075 0.0027 0.0013 0.0006 0.0022 0.0169 0.0013 0.0015 0.0005 0.0005 0.0015 0.0016 0.0041 0.0027 0.0072 0.0007 0.0051
Vacuole 0.0448 0.0662 0.0445 0.0639 0.0305 0.0224 0.0213 0.0526 0.0121 0.0286 0.0053 0.0201 0.0003 0.0389 0.023 0.0192 0.006 0.0299
Vacuole Periphery 0.0082 0.008 0.0079 0.0143 0.0077 0.0053 0.0043 0.0076 0.0036 0.0167 0.0013 0.0039 0.0002 0.0048 0.0043 0.0054 0.0025 0.0045

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 5.6877 4.0675 6.2042 10.5604 5.5717 5.0529 8.9679 10.255 8.8948 10.9127
Translational Efficiency

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
720 1023 574 1315 1976 1163 1198 1578 2696 2186 1772 2893

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 752.26 710.33 897.37 843.04 641.27 779.09 784.61 867.42 670.91 746.91 821.14 856.34
Standard Deviation 98.17 101.67 97.91 117.65 74.95 101.83 117.32 121.64 95.41 107.38 123.27 120.45
Intensity Change Log 2 -0.082742 0.254472 0.164370 0.280858 0.291044 0.435799 0.096007 0.271416 0.295640

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000710 0.000349 0.000296 0.000639 0.000458 0.000248 0.000374 0.000338 0.000525 0.000295 0.000349 0.000475
Bud Neck 0.032575 0.016669 0.016796 0.013160 0.040544 0.020282 0.011253 0.007691 0.038416 0.018591 0.013048 0.010177
Bud Site 0.018199 0.016231 0.009867 0.022229 0.009356 0.005984 0.010513 0.011478 0.011718 0.010779 0.010304 0.016365
Cell Periphery 0.000170 0.002080 0.000062 0.000041 0.000195 0.000188 0.000034 0.000036 0.000189 0.001073 0.000043 0.000039
Cytoplasm 0.050879 0.067015 0.010339 0.023012 0.071466 0.024330 0.019372 0.011378 0.065968 0.044306 0.016446 0.016666
Cytoplasmic Foci 0.088883 0.241447* 0.069318 0.123854 0.313553* 0.098869 0.124137 0.118334 0.253552* 0.165593 0.106380 0.120843
Eisosomes 0.000098 0.000069 0.000049 0.000029 0.000429 0.000075 0.000034 0.000021 0.000341 0.000072 0.000039 0.000024
Endoplasmic Reticulum 0.001526 0.000627 0.002587 0.000906 0.002083 0.001807 0.000925 0.000674 0.001935 0.001255 0.001463 0.000780
Endosome 0.444908* 0.426877* 0.445850* 0.444312* 0.284774* 0.516405* 0.531591* 0.612695* 0.327540* 0.474508* 0.503817* 0.536158*
Golgi 0.009405 0.036985 0.010034 0.014711 0.091733 0.025441 0.012706 0.023509 0.069747 0.030843 0.011841 0.019510
Lipid Particles 0.017835 0.014518 0.007159 0.008848 0.052130 0.026865 0.006493 0.008687 0.042971 0.021087 0.006709 0.008760
Mitochondria 0.007311 0.021411 0.004853 0.004037 0.045241 0.031972 0.004213 0.004174 0.035111 0.027030 0.004420 0.004112
Mitotic Spindle 0.010883 0.006105 0.028027 0.059065 0.000762 0.005119 0.052892 0.029545 0.003465 0.005581 0.044837 0.042963
None 0.000699 0.001192 0.000436 0.000349 0.001474 0.002228 0.000212 0.000894 0.001267 0.001743 0.000284 0.000646
Nuclear Periphery 0.001180 0.000210 0.002590 0.001225 0.000185 0.001126 0.001382 0.000692 0.000451 0.000697 0.001773 0.000934
Nuclear Periphery Foci 0.002564 0.000663 0.012372 0.006922 0.000325 0.000938 0.009094 0.003681 0.000923 0.000809 0.010156 0.005154
Nucleolus 0.011356 0.001568 0.023300 0.005843 0.000763 0.003582 0.010965 0.002395 0.003592 0.002639 0.014961 0.003962
Nucleus 0.053369 0.005344 0.028653 0.008154 0.006846 0.011068 0.010174 0.001970 0.019271 0.008389 0.016160 0.004781
Peroxisomes 0.030465 0.026257 0.017777 0.028225 0.021436 0.011971 0.033103 0.025558 0.023847 0.018657 0.028139 0.026770
Vacuole 0.211598* 0.101939 0.274021* 0.224039* 0.046054 0.183026 0.151415 0.127212 0.090265 0.145079 0.191131 0.171224
Vacuole Periphery 0.005386 0.012443 0.035612 0.010400 0.010190 0.028477 0.009119 0.009036 0.008907 0.020974 0.017701 0.009656

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.86 0.93 0.02 -1.64 -1.75 1.56 0.01 0.71 -0.94 0.71 1.64 0.77 0.07 -1.86 -0.82
Bud Neck 4.99 4.77 6.03 1.40 1.49 6.67 10.79 12.70 6.47 4.04 8.70 11.63 13.39 5.94 3.13
Bud Site 0.57 2.43 -1.23 -2.38 -4.99 2.65 -1.45 -1.75 -4.36 -0.48 0.67 0.57 -3.21 -4.13 -4.25
Cell Periphery -1.49 3.66 4.54 1.59 3.22 0.13 9.74 9.77 4.00 0.28 -1.47 10.08 10.67 1.72 2.08
Cytoplasm -2.09 7.92 3.88 6.82 -7.00 12.78 11.90 17.22 4.29 3.92 5.93 14.71 15.31 8.04 -0.56
Cytoplasmic Foci -20.49 3.72 -5.19 17.45 -9.63 35.67* 32.09 33.87 -3.95 0.66 15.84 29.88 28.16 10.48 -3.89
Eisosomes 3.42 5.70 9.21 8.93 5.36 9.86 11.05 11.56 7.56 6.35 10.17 11.39 12.10 10.97 7.83
Endoplasmic Reticulum 1.85 -2.08 1.17 -2.87 5.86 0.63 2.63 3.84 3.64 2.91 2.13 0.96 3.85 2.56 6.03
Endosome 0.37 -0.57 1.35 1.21 2.10 -21.26 -20.22 -33.73* -7.31 -9.14 -17.38 -17.14 -23.65 -4.75 -3.86
Golgi -9.22 -0.45 -1.94 8.87 -1.84 17.69 25.11 20.49 1.03 -7.38 13.42 23.27 19.82 6.73 -6.59
Lipid Particles 1.01 4.05 3.58 3.16 -0.96 7.23 18.24 16.72 6.76 -1.34 8.89 18.26 17.05 7.39 -1.69
Mitochondria -5.56 1.19 1.76 8.67 1.13 4.19 18.97 19.37 11.56 -0.03 3.57 18.15 18.61 14.42 0.64
Mitotic Spindle 1.57 -3.43 -9.89 -12.16 -5.61 -3.39 -12.64 -10.70 -8.43 4.69 -2.13 -12.99 -15.65 -14.68 0.35
None -0.95 1.23 1.58 2.14 0.44 -0.70 3.05 1.01 1.30 -1.19 -0.69 3.20 1.64 1.87 -1.12
Nuclear Periphery 3.00 -2.70 -0.65 -8.01 2.85 -4.84 -7.36 -4.63 1.57 3.86 -1.50 -7.46 -4.56 -2.97 4.25
Nuclear Periphery Foci 3.11 -4.38 -3.02 -4.95 2.52 -1.87 -8.97 -5.06 -3.77 4.95 0.50 -9.42 -6.06 -6.24 4.81
Nucleolus 5.73 -3.24 2.94 -5.67 5.58 -4.12 -7.84 -3.59 0.82 6.12 1.81 -8.31 -0.91 -2.91 7.94
Nucleus 8.74 3.53 7.83 -2.64 4.66 -2.93 -3.50 6.66 6.10 6.35 6.05 0.50 8.05 2.60 6.83
Peroxisomes 1.14 3.64 0.84 -0.36 -3.26 5.87 -4.21 -2.13 -6.51 2.20 3.21 -1.91 -1.63 -4.43 0.50
Vacuole 10.57 -3.65 -2.00 -15.87 2.13 -19.52 -19.27 -16.21 6.21 4.74 -9.52 -17.19 -16.12 -6.18 2.62
Vacuole Periphery -5.83 -11.64 -4.28 2.01 9.61 -10.66 1.55 1.47 11.46 -0.30 -10.76 -7.24 -0.43 10.36 6.90
Standard name
Human Ortholog
Description Serine/threonine protein kinase involved in vacuolar protein sorting; functions as a membrane-associated complex with Vps34p; active form recruits Vps34p to the Golgi membrane; interacts with the GDP-bound form of Gpa1p; myristoylated; a fraction is localized, with Vps34p, to nuclear pores at nucleus-vacuole junctions and may facilitate transcription elongation for genes positioned at the nuclear periphery
Localization
Cell Percentages endosome (46%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Vps15

Vps15


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Vps15-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available