Standard name
Description GID Complex regulatory subunit; binds GID Complex in response to glucose through interactions with complex member Vid28p; regulates fructose-1,6-bisphosphatase (FBPase) targeting to the vacuole; promotes proteasome-dependent catabolite degradation of FBPase; peripheral membrane protein located at Vid (vacuole import and degradation) vesicles

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.95 0.93 0.98 0.93 0.89 0.81 0.49 0.56 0.58 0.54 0.59 0.94 0.9 0.89 0.95 0.9 0.92 0.86 0.78 0.91 0.82 0.75 0.62
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.07 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Mitochondria 0 0 0 0 0 0.08 0.12 0.11 0.11 0.28 0.21 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0.07 0.06 0.08 0.09 0.06 0.07 0 0 0 0 0 0 0.08 0.13 0 0.08 0.1 0.08
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.09 0 0 0.14 0.11 0.1 0.38 0.31 0.27 0.2 0.18 0.1 0.13 0.17 0.11 0.23 0.11 0 0.05 0 0.05 0.07 0.1
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 2 1 2 1 3 0 0 0 0 0 0 1 0 0 0 0 2
Bud 0 0 0 0 1 5 5 6 2 9 13 0 0 0 0 0 0 3 0 1 1 3 5
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 3 0 2 3 3 0 4 7 14 22 0 0 1 0 1 0 0 0 0 0 0 0
Cytoplasm 309 139 377 320 462 533 393 422 424 356 545 248 279 304 59 78 60 282 131 340 148 267 210
Endoplasmic Reticulum 3 2 0 2 7 5 11 9 22 6 19 3 6 1 3 6 1 2 1 2 2 7 11
Endosome 0 0 0 0 0 3 10 4 3 3 1 0 0 1 0 0 0 0 0 2 0 3 18
Golgi 0 2 1 0 2 0 0 0 0 0 0 2 0 0 0 0 2 1 0 0 0 1 15
Mitochondria 0 0 0 1 8 50 94 83 80 183 190 0 1 0 0 0 0 0 0 0 0 0 3
Nucleus 2 1 2 3 7 43 48 60 65 41 64 1 2 4 0 0 1 26 22 15 15 34 27
Nuclear Periphery 0 0 0 0 0 0 2 0 3 0 2 0 0 0 0 0 0 1 0 1 0 3 1
Nucleolus 0 8 0 0 0 0 8 6 2 0 2 1 0 0 0 1 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 4
Vac/Vac Membrane 30 4 12 49 58 64 307 231 199 129 161 26 41 57 7 20 7 3 8 3 9 26 34
Unique Cell Count 326 150 385 344 517 655 801 753 733 656 917 265 310 342 62 87 65 327 168 374 181 355 342
Labelled Cell Count 344 159 392 377 548 706 880 826 809 742 1022 281 329 368 69 106 71 327 168 374 181 355 342


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 19.5 23.2 13.6 15.2 12.3 9.0 7.9 8.1 8.4 8.6 9.2 21.7 19.8 19.3 17.6 20.5 19.9 20.3 20.2 20.1
Std Deviation (1e-4) 3.9 4.2 2.7 3.3 2.5 1.8 1.6 1.8 1.9 2.0 2.4 4.7 4.1 3.8 4.3 4.8 5.6 4.1 4.7 4.8
Intensity Change (Log2) 0.16 -0.15 -0.59 -0.79 -0.75 -0.71 -0.66 -0.57 0.67 0.54 0.5 0.37 0.59 0.55 0.57 0.57 0.56


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 2.9 3.1 0 0 0 0 0 0
Cytoplasm -3.2 -5.0 -7.8 -16.5 -14.6 -14.1 -14.9 -13.9 -2.8 -4.5 -5.0 -1.3 -3.7 -2.5
Endoplasmic Reticulum 0 0 0 0 0 3.4 0 2.8 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 5.6 7.0 6.8 6.7 11.4 9.7 0 0 0 0 0 0
Nucleus 0 0 4.6 4.4 5.2 5.6 4.5 4.8 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 5.4 4.5 4.0 12.8 10.7 9.8 7.5 7.0 3.6 5.0 6.2 0 6.7 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Vid24-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available