Standard name
Description General transcriptional co-repressor; acts together with Tup1p; also acts as part of a transcriptional co-activator complex that recruits the SWI/SNF and SAGA complexes to promoters; can form the prion [OCT+]

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0.06 0 0 0 0 0.08 0.09 0.07 0.05 0.09 0.05 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.05 0.05 0.06 0.12 0.15 0.09 0.1 0 0 0 0.05 0 0.08 0 0 0 0 0 0
Nucleus 0.69 0.68 0.72 0.89 0.73 0.65 0.66 0.58 0.54 0.56 0.54 0.47 0.72 0.76 0.68 0.75 0.7 0.78 0.6 0.68 0.64 0.59 0.65 0.51
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.61 0.46 0.54 0.37 0.49 0.48 0.56 0.55 0.6 0.66 0.7 0.71 0.62 0.53 0.53 0.37 0.35 0.32 0.33 0.24 0.31 0.33 0.25 0.41
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 1 0 0 2 0 1 0 1 0 1 1 1 0 0 2 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 2 1 0 1 0 4 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 2 0 5 5 8 11 6 2 7 1 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2 2 6 0 2 7 5 17 8 6 3 5 3 10 6 8 9 6 1 0 0 0 0 2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 3 0 1 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Mitochondria 0 1 6 2 4 8 13 17 22 30 16 19 1 0 2 8 4 10 0 1 1 0 0 0
Nucleus 76 34 130 108 99 115 166 166 99 115 93 89 28 84 60 123 73 102 74 54 122 29 79 73
Nuclear Periphery 0 0 0 1 2 1 2 1 1 1 4 3 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 67 23 97 45 67 85 142 155 109 135 120 134 24 58 47 61 37 42 41 19 60 16 30 58
Peroxisomes 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 1 0 0 0 0 2 1 0 0 1 0 1 0 3 0 2 0 0 0 0 0 0
Vac/Vac Membrane 0 1 1 0 0 0 1 1 0 0 0 0 0 0 0 1 1 4 1 1 6 1 7 6
Unique Cell Count 110 50 180 121 136 176 252 284 182 204 171 188 39 110 88 165 105 130 123 81 193 50 122 143
Labelled Cell Count 145 61 241 158 175 221 336 371 251 295 238 263 57 155 116 208 125 167 123 81 193 50 122 143


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 16.1 10.7 9.3 9.8 9.8 10.0 9.0 9.3 9.0 8.7 9.1 8.4 12.1 13.4 11.5 17.2 18.1 19.4 12.3 14.3 15.5
Std Deviation (1e-4) 5.5 3.9 3.1 2.6 2.6 3.8 2.3 3.0 3.1 2.9 3.6 3.2 4.1 6.0 3.9 5.9 6.8 6.3 2.5 3.4 5.9
Intensity Change (Log2) 0.08 0.07 0.11 -0.05 -0.0 -0.05 -0.1 -0.03 -0.14 0.38 0.53 0.31 0.89 0.96 1.06 0.4 0.62 0.74


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 3.3 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.3 0 1.3 0.5 -0.2 0 -0.4 0 2.1 0 0.7 1.9 0.6
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.6 0.9 1.3 3.1 3.8 2.3 2.6 0 0 0 0.7 0 1.7
Nucleus 3.6 0.1 -1.4 -1.4 -3.0 -3.5 -3.2 -3.5 -4.9 -0.1 0.8 -0.7 0.5 -0.5 1.2
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -2.8 -0.8 -1.1 0.5 0.1 1.2 2.5 3.1 3.4 0.9 -0.2 -0.1 -3.2 -3.0 -3.8
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cyc8-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available