Standard name
Human Ortholog
Description General transcriptional co-repressor; acts together with Tup1p; also acts as part of a transcriptional co-activator complex that recruits the SWI/SNF and SAGA complexes to promoters; can form the prion [OCT+]

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0.06 0 0 0 0 0.08 0.09 0.07 0.05 0.09 0.05 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.05 0.05 0.06 0.12 0.15 0.09 0.1 0 0 0 0.05 0 0.08 0 0 0 0 0 0
Nucleus 0.69 0.68 0.72 0.89 0.73 0.65 0.66 0.58 0.54 0.56 0.54 0.47 0.72 0.76 0.68 0.75 0.7 0.78 0.6 0.68 0.64 0.59 0.65 0.51
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.61 0.46 0.54 0.37 0.49 0.48 0.56 0.55 0.6 0.66 0.7 0.71 0.62 0.53 0.53 0.37 0.35 0.32 0.33 0.24 0.31 0.33 0.25 0.41
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 1 0 0 2 0 1 0 1 0 1 1 1 0 0 2 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 2 1 0 1 0 4 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 2 0 5 5 8 11 6 2 7 1 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2 2 6 0 2 7 5 17 8 6 3 5 3 10 6 8 9 6 1 0 0 0 0 2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 3 0 1 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Mitochondria 0 1 6 2 4 8 13 17 22 30 16 19 1 0 2 8 4 10 0 1 1 0 0 0
Nucleus 76 34 130 108 99 115 166 166 99 115 93 89 28 84 60 123 73 102 74 54 122 29 79 73
Nuclear Periphery 0 0 0 1 2 1 2 1 1 1 4 3 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 67 23 97 45 67 85 142 155 109 135 120 134 24 58 47 61 37 42 41 19 60 16 30 58
Peroxisomes 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 1 0 0 0 0 2 1 0 0 1 0 1 0 3 0 2 0 0 0 0 0 0
Vac/Vac Membrane 0 1 1 0 0 0 1 1 0 0 0 0 0 0 0 1 1 4 1 1 6 1 7 6
Unique Cell Count 110 50 180 121 136 176 252 284 182 204 171 188 39 110 88 165 105 130 123 81 193 50 122 143
Labelled Cell Count 145 61 241 158 175 221 336 371 251 295 238 263 57 155 116 208 125 167 123 81 193 50 122 143


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 16.1 10.7 9.3 9.8 9.8 10.0 9.0 9.3 9.0 8.7 9.1 8.4 12.1 13.4 11.5 17.2 18.1 19.4 12.3 14.3 15.5
Std Deviation (1e-4) 5.5 3.9 3.1 2.6 2.6 3.8 2.3 3.0 3.1 2.9 3.6 3.2 4.1 6.0 3.9 5.9 6.8 6.3 2.5 3.4 5.9
Intensity Change (Log2) 0.08 0.07 0.11 -0.05 -0.0 -0.05 -0.1 -0.03 -0.14 0.38 0.53 0.31 0.89 0.96 1.06 0.4 0.62 0.74


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 3.3 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.3 0 1.3 0.5 -0.2 0 -0.4 0 2.1 0 0.7 1.9 0.6
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.6 0.9 1.3 3.1 3.8 2.3 2.6 0 0 0 0.7 0 1.7
Nucleus 3.6 0.1 -1.4 -1.4 -3.0 -3.5 -3.2 -3.5 -4.9 -0.1 0.8 -0.7 0.5 -0.5 1.2
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -2.8 -0.8 -1.1 0.5 0.1 1.2 2.5 3.1 3.4 0.9 -0.2 -0.1 -3.2 -3.0 -3.8
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 14.43 15.1094 13.8222 15.9156 12.4484 13.7113 12.5764 15.5585 12.7024 12.0396 10.6287 13.1211 18.0576 15.443 15.8215 14.5913 16.3715 15.0264
Actin 0.0038 0 0.0216 0.0077 0.006 0 0.0211 0 0.0018 0.002 0.0142 0 0 0 0 0 0 0
Bud 0.0021 0 0.0002 0.0001 0.0002 0 0.0004 0 0.0003 0.0001 0.0001 0 0 0 0 0 0.0001 0
Bud Neck 0.003 0 0.0008 0.0001 0.0002 0.0002 0.0025 0 0.0002 0.0007 0.0002 0.0002 0 0 0 0 0.0002 0.0003
Bud Periphery 0.0021 0 0.0003 0.0003 0.0004 0.0001 0.0007 0 0.0004 0.0001 0.0002 0 0 0 0 0 0.0002 0
Bud Site 0.0047 0 0.0023 0.0005 0.0007 0.0001 0.0026 0.0001 0.0016 0.0023 0.0015 0 0 0 0 0 0.0001 0
Cell Periphery 0.0011 0 0.0002 0.0001 0.0003 0.0001 0.0007 0 0.0002 0.0001 0.0002 0 0 0 0 0 0.0002 0
Cytoplasm 0.01 0 0.0004 0 0.0002 0 0.0046 0 0.0015 0.0003 0.0064 0.0003 0 0 0 0 0 0
Cytoplasmic Foci 0.0047 0 0.0014 0.0002 0.0186 0.0037 0.0161 0 0.0027 0.0105 0.0205 0.0061 0 0 0 0 0 0
Eisosomes 0.0002 0 0.0003 0.0003 0.0003 0 0.0004 0 0 0.0001 0.0004 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0029 0 0.0006 0 0.0003 0 0.0019 0 0.0009 0.0001 0.0036 0.0001 0 0 0 0 0 0
Endosome 0.0188 0 0.0021 0.0002 0.0083 0 0.0141 0 0.0027 0.0105 0.0156 0.0004 0 0 0 0 0 0
Golgi 0.0064 0 0.0035 0.0004 0.0087 0 0.0053 0 0.0007 0.0057 0.0059 0.0001 0 0 0 0 0 0
Lipid Particles 0.0264 0 0.0016 0.0014 0.0189 0.0011 0.0128 0 0.0038 0.0165 0.0111 0.0018 0 0 0 0 0 0
Mitochondria 0.0137 0.0001 0.0062 0.0242 0.028 0.0007 0.0035 0.0001 0.0061 0.0012 0.0014 0.0001 0.0003 0.0001 0.0001 0.0001 0.0009 0.0001
None 0.0242 0 0.0016 0.0001 0.001 0.0001 0.0044 0 0.0008 0.0004 0.0081 0.0003 0 0 0 0 0 0
Nuclear Periphery 0.0086 0.0001 0.0005 0.0001 0.0011 0 0.0044 0.0001 0.0052 0.0004 0.0024 0.0011 0.0001 0.0002 0.0002 0.0004 0.0002 0
Nucleolus 0.2315 0.1071 0.1052 0.1497 0.517 0.3699 0.1925 0.1239 0.087 0.1413 0.3339 0.2827 0.1655 0.0912 0.0801 0.1005 0.542 0.3234
Nucleus 0.6189 0.8924 0.8475 0.8111 0.381 0.623 0.6893 0.8753 0.8672 0.7984 0.507 0.7014 0.8337 0.8996 0.9193 0.8986 0.4553 0.6758
Peroxisomes 0.0057 0 0.0023 0.0029 0.0048 0 0.0118 0 0.0045 0.0044 0.057 0.0002 0 0 0 0 0 0
Punctate Nuclear 0.0031 0.0001 0.0008 0.0001 0.002 0.0005 0.0074 0.0003 0.0094 0.0039 0.0092 0.0047 0.0001 0.0088 0.0002 0.0002 0.0004 0.0002
Vacuole 0.005 0 0.0003 0.0001 0.0008 0.0002 0.0021 0 0.0012 0.0006 0.0005 0.0001 0.0001 0 0 0 0.0003 0
Vacuole Periphery 0.003 0 0.0003 0.0002 0.0014 0.0002 0.0014 0 0.0016 0.0004 0.0003 0.0002 0.0001 0 0 0 0.0002 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 55.1512 44.8816 29.5196 47.054 57.2048 39.915 43.7468 53.1355 36.5174 68.1059
Translational Efficiency 1.2963 1.2674 1.368 0.7138 1.2147 1.4872 1.0769 0.7772 1.167 0.9221

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
380 80 165 273 1495 2160 164 21 1875 2240 329 294

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 989.15 3049.28 2008.77 1427.43 1119.95 1254.13 1925.87 1772.03 1093.44 1318.24 1967.45 1452.04
Standard Deviation 174.72 4477.43 379.21 344.23 236.45 254.59 319.59 372.85 231.36 943.11 353.20 357.54
Intensity Change Log 2 1.624207 1.022051 0.529159 0.163253 0.782076 0.661969 1.028853 0.899604 0.601201

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000441 0.000788 0.001189 0.001557 0.000498 0.000457 0.001402 0.000987 0.000486 0.000469 0.001295 0.001517
Bud Neck 0.001893 0.005332 0.007712 0.013636 0.002988 0.003908 0.010736 0.010483 0.002766 0.003959 0.009220 0.013411
Bud Site 0.000269 0.001294 0.001418 0.004909 0.000327 0.000576 0.001410 0.000798 0.000315 0.000601 0.001414 0.004615
Cell Periphery 0.000027 0.003478 0.000026 0.000194 0.000022 0.000063 0.000032 0.000041 0.000023 0.000185 0.000029 0.000184
Cytoplasm 0.000872 0.067487 0.000023 0.001324 0.000140 0.000135 0.000056 0.000547 0.000288 0.002541 0.000039 0.001268
Cytoplasmic Foci 0.000770 0.004004 0.000985 0.007727 0.000073 0.000322 0.000559 0.000175 0.000214 0.000453 0.000773 0.007187
Eisosomes 0.000053 0.000102 0.000272 0.000085 0.000116 0.000145 0.000210 0.000093 0.000103 0.000144 0.000242 0.000086
Endoplasmic Reticulum 0.000527 0.000498 0.000382 0.001475 0.000537 0.000456 0.000498 0.000301 0.000535 0.000458 0.000440 0.001391
Endosome 0.000104 0.000857 0.000012 0.006593 0.000021 0.000481 0.000018 0.000025 0.000038 0.000494 0.000015 0.006124
Golgi 0.000235 0.010437 0.000169 0.001823 0.000059 0.000313 0.000174 0.000197 0.000095 0.000675 0.000171 0.001707
Lipid Particles 0.000151 0.004103 0.000018 0.004926 0.000104 0.000170 0.000022 0.000018 0.000113 0.000311 0.000020 0.004575
Mitochondria 0.001354 0.004508 0.002721 0.005243 0.000774 0.000708 0.003224 0.003302 0.000891 0.000844 0.002971 0.005105
Mitotic Spindle 0.003639 0.013273 0.000404 0.025785 0.000804 0.000670 0.000876 0.000459 0.001379 0.001120 0.000639 0.023976
None 0.000801 0.001298 0.000634 0.004208 0.000705 0.000575 0.000929 0.000798 0.000725 0.000601 0.000781 0.003965
Nuclear Periphery 0.000027 0.000267 0.000043 0.000791 0.000027 0.000045 0.000087 0.000228 0.000027 0.000053 0.000065 0.000751
Nuclear Periphery Foci 0.000074 0.003048 0.000007 0.000989 0.000583 0.000268 0.000015 0.000060 0.000480 0.000368 0.000011 0.000923
Nucleolus 0.197751 0.148379 0.196047 0.115522 0.172702 0.157158 0.146012 0.145774 0.177779 0.156845 0.171105 0.117682
Nucleus 0.786881* 0.684412* 0.727400* 0.790603* 0.813697* 0.828869* 0.804175* 0.829824* 0.808262* 0.823710* 0.765671* 0.793405*
Peroxisomes 0.003787 0.016239 0.059771 0.008600 0.005306 0.003917 0.028792 0.004727 0.004998 0.004357 0.044329 0.008323
Vacuole 0.000169 0.022443 0.000425 0.002509 0.000155 0.000387 0.000474 0.000641 0.000158 0.001175 0.000450 0.002376
Vacuole Periphery 0.000174 0.007753 0.000340 0.001499 0.000362 0.000374 0.000299 0.000523 0.000324 0.000638 0.000319 0.001430

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.60 -11.81 -12.57 -5.83 -3.98 0.95 -12.40 -3.60 -3.76 2.59 0.34 -16.90 -12.82 -12.36 -2.69
Bud Neck -2.23 -10.91 -6.54 -3.80 -3.33 -2.33 -10.52 -3.71 -3.26 0.43 -3.16 -13.69 -6.41 -5.68 -2.36
Bud Site -1.82 -8.47 -3.64 -2.45 -2.37 -2.22 -9.46 -1.55 -1.03 1.04 -2.58 -12.83 -3.66 -3.35 -2.31
Cell Periphery -2.83 -0.31 -3.03 2.72 -3.05 -2.35 -3.56 -1.27 0.06 -0.75 -3.37 -2.84 -3.30 0.55 -3.00
Cytoplasm -2.80 1.03 -0.37 2.79 -4.47 -0.36 0.70 -0.99 -0.97 -1.04 -2.54 1.30 -2.90 1.43 -4.38
Cytoplasmic Foci -1.69 -0.50 -1.33 -0.22 -1.24 -1.98 -6.60 -0.98 -0.38 0.19 -1.27 -4.03 -1.56 -1.46 -1.32
Eisosomes -3.45 -14.08 -5.06 0.94 11.44 -4.98 -8.65 1.57 3.08 6.07 -7.50 -14.19 2.23 7.59 14.16
Endoplasmic Reticulum 0.32 1.37 -5.29 -5.29 -5.78 1.26 -0.82 2.30 1.53 3.03 1.36 0.33 -5.16 -5.59 -5.30
Endosome -0.99 1.13 -2.15 -1.63 -2.20 -1.98 -0.90 -0.85 1.77 -0.73 -2.01 1.03 -2.18 -1.89 -2.19
Golgi -2.83 0.32 -2.19 2.50 -2.32 -1.63 -7.69 -1.11 -0.48 -0.72 -2.86 -4.32 -2.59 -1.21 -2.34
Lipid Particles -2.14 1.03 -1.23 0.39 -1.29 -0.54 1.03 0.67 1.17 -0.43 -1.53 1.41 -1.24 -1.15 -1.29
Mitochondria -0.96 -2.61 -3.47 -0.08 -2.26 0.62 -11.78 -3.08 -3.16 0.29 0.26 -12.45 -5.08 -5.14 -1.82
Mitotic Spindle -0.75 1.21 -3.37 -0.96 -4.14 0.26 -0.72 0.43 0.19 1.00 0.33 0.32 -3.95 -4.01 -4.01
None -1.47 0.68 -3.43 -2.85 -3.60 0.88 -2.70 -1.19 -1.56 -0.11 0.88 -1.42 -3.57 -3.68 -3.44
Nuclear Periphery -2.93 -2.60 -4.30 -2.51 -4.14 -1.57 -4.27 -2.24 -2.07 -1.25 -2.26 -4.89 -4.41 -4.22 -3.94
Nuclear Periphery Foci -2.30 2.88 -2.87 1.73 -3.15 0.65 1.19 0.74 0.27 -0.93 0.30 1.24 -0.50 -1.37 -3.17
Nucleolus 2.39 -0.11 4.33 0.53 3.67 2.81 1.38 1.05 0.65 0.58 4.04 0.06 3.65 1.82 2.96
Nucleus 2.79 3.54 0.61 -2.35 -2.41 -2.58 1.54 -0.61 -0.23 -1.13 -2.71 4.45 1.75 2.73 -1.35
Peroxisomes -2.14 -9.35 -3.87 1.25 8.40 4.08 -7.06 0.66 -0.47 6.89 1.77 -11.12 -2.92 -3.44 9.73
Vacuole -1.89 -2.85 -2.94 1.69 -2.58 -2.27 -3.64 -1.10 -0.94 -0.73 -2.30 -4.88 -3.08 -1.47 -2.53
Vacuole Periphery -1.64 -3.13 -2.13 1.42 -1.75 0.01 0.22 -0.90 -1.07 -1.24 -1.23 -0.04 -1.73 -1.08 -1.84