Standard name
Description Alpha aminoadipate reductase; catalyzes the reduction of alpha-aminoadipate to alpha-aminoadipate 6-semialdehyde, which is the fifth step in biosynthesis of lysine; activation requires posttranslational phosphopantetheinylation by Lys5p

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0.07 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0.06 0.06 0.08 0.16 0.13 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 0.99 0.99 0.99 0.95 0.96 0.86 0.88 0.86 0.82 0.78 0.99 0.98 0.98 0.96 0.96 0.98 0.97 0.97 0.97 0.93 0.94 0.91
Endoplasmic Reticulum 0 0 0 0 0.05 0 0.11 0.07 0.08 0.06 0.11 0 0 0 0 0.05 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 2 8 8 14 14 0 0 0 1 1 0 0 0 0 0 0 2
Bud 0 9 0 0 0 0 1 1 3 1 2 0 1 1 3 0 1 1 4 0 7 4 11
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 6 8 4 2 9 10 16 18 20 31 33 7 5 13 13 6 6 0 0 0 0 0 0
Cytoplasm 365 487 369 272 329 224 233 247 228 159 196 449 537 584 439 321 397 344 476 367 353 430 566
Endoplasmic Reticulum 0 2 7 3 16 10 30 20 20 11 27 4 8 13 20 17 21 1 1 3 7 15 22
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 1 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 1 3 0 1 0 0 1 2 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 3 6 12 7 0 0 0 0 1 1 1 0 0 0 0 0
Nucleus 0 1 0 0 0 0 1 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Nucleolus 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Peroxisomes 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 0 0 0 0 0 0 0
Vac/Vac Membrane 0 1 0 0 0 0 0 0 0 2 2 0 0 0 13 9 5 1 6 2 2 2 3
Unique Cell Count 366 491 374 276 346 234 272 280 264 195 252 454 546 598 459 333 404 356 493 377 378 459 619
Labelled Cell Count 371 509 380 277 354 244 283 299 288 230 282 460 551 612 497 358 434 356 493 377 378 459 619


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 167.3 140.7 86.5 85.9 72.7 55.1 47.2 40.6 36.4 30.2 30.3 105.1 96.4 83.8 122.2 132.6 125.8 118.2 129.6 124.0
Std Deviation (1e-4) 35.0 30.8 18.4 17.5 15.7 12.8 12.7 10.4 9.3 8.7 8.0 25.7 21.1 19.1 49.0 54.8 50.5 29.9 29.8 33.8
Intensity Change (Log2) -0.01 -0.25 -0.65 -0.87 -1.09 -1.25 -1.52 -1.51 0.28 0.16 -0.05 0.5 0.62 0.54 0.45 0.58 0.52


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 4.6 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 1.5 2.6 3.5 3.8 4.3 7.0 6.3 0 0 1.3 1.8 0 0
Cytoplasm -0.1 -2.8 -2.3 -6.5 -5.7 -6.2 -7.5 -8.6 0.3 -0.4 -1.1 -2.5 -2.0 -0.4
Endoplasmic Reticulum 0 2.1 1.7 4.9 3.4 3.5 2.4 4.8 0 -0.5 0.3 2.0 2.4 2.5
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 3.3 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Lys2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available