Standard name
Human Ortholog
Description Alpha aminoadipate reductase; catalyzes the reduction of alpha-aminoadipate to alpha-aminoadipate 6-semialdehyde, which is the fifth step in biosynthesis of lysine; activation requires posttranslational phosphopantetheinylation by Lys5p

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0.07 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0.06 0.06 0.08 0.16 0.13 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 0.99 0.99 0.99 0.95 0.96 0.86 0.88 0.86 0.82 0.78 0.99 0.98 0.98 0.96 0.96 0.98 0.97 0.97 0.97 0.93 0.94 0.91
Endoplasmic Reticulum 0 0 0 0 0.05 0 0.11 0.07 0.08 0.06 0.11 0 0 0 0 0.05 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 2 8 8 14 14 0 0 0 1 1 0 0 0 0 0 0 2
Bud 0 9 0 0 0 0 1 1 3 1 2 0 1 1 3 0 1 1 4 0 7 4 11
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 6 8 4 2 9 10 16 18 20 31 33 7 5 13 13 6 6 0 0 0 0 0 0
Cytoplasm 365 487 369 272 329 224 233 247 228 159 196 449 537 584 439 321 397 344 476 367 353 430 566
Endoplasmic Reticulum 0 2 7 3 16 10 30 20 20 11 27 4 8 13 20 17 21 1 1 3 7 15 22
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 1 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 1 3 0 1 0 0 1 2 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 3 6 12 7 0 0 0 0 1 1 1 0 0 0 0 0
Nucleus 0 1 0 0 0 0 1 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Nucleolus 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Peroxisomes 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 0 0 0 0 0 0 0
Vac/Vac Membrane 0 1 0 0 0 0 0 0 0 2 2 0 0 0 13 9 5 1 6 2 2 2 3
Unique Cell Count 366 491 374 276 346 234 272 280 264 195 252 454 546 598 459 333 404 356 493 377 378 459 619
Labelled Cell Count 371 509 380 277 354 244 283 299 288 230 282 460 551 612 497 358 434 356 493 377 378 459 619


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 167.3 140.7 86.5 85.9 72.7 55.1 47.2 40.6 36.4 30.2 30.3 105.1 96.4 83.8 122.2 132.6 125.8 118.2 129.6 124.0
Std Deviation (1e-4) 35.0 30.8 18.4 17.5 15.7 12.8 12.7 10.4 9.3 8.7 8.0 25.7 21.1 19.1 49.0 54.8 50.5 29.9 29.8 33.8
Intensity Change (Log2) -0.01 -0.25 -0.65 -0.87 -1.09 -1.25 -1.52 -1.51 0.28 0.16 -0.05 0.5 0.62 0.54 0.45 0.58 0.52


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 4.6 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 1.5 2.6 3.5 3.8 4.3 7.0 6.3 0 0 1.3 1.8 0 0
Cytoplasm -0.1 -2.8 -2.3 -6.5 -5.7 -6.2 -7.5 -8.6 0.3 -0.4 -1.1 -2.5 -2.0 -0.4
Endoplasmic Reticulum 0 2.1 1.7 4.9 3.4 3.5 2.4 4.8 0 -0.5 0.3 2.0 2.4 2.5
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 3.3 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 165.0464 196.2947 190.156 179.3442 181.2144 186.6416 115.8505 182.8007 164.8264 164.5933 174.1823 152.0211 154.4621 203.3966 184.6697 176.0174 163.7855 181.3395
Actin 0.0002 0.0002 0.0002 0.0001 0.0002 0.0001 0.0068 0.0005 0.0046 0.0001 0.0004 0.0005 0.0008 0.0001 0.0128 0.0002 0.0002 0.0006
Bud 0.0004 0.0005 0.0006 0.0007 0.0004 0.0004 0.0034 0.0005 0.0016 0.001 0.0005 0.0008 0.0017 0.0001 0.0017 0.0008 0.0002 0.0002
Bud Neck 0.0009 0.0014 0.0016 0.001 0.0026 0.0044 0.0006 0.0004 0.0008 0.0008 0.0013 0.002 0.0005 0.0003 0.0009 0.0007 0.0003 0.0011
Bud Periphery 0.0006 0.0006 0.0007 0.0004 0.0007 0.0002 0.0043 0.0003 0.0008 0.0005 0.0002 0.0009 0.0011 0 0.0022 0.0006 0 0.0001
Bud Site 0.0002 0.0007 0.0003 0.0002 0.0001 0 0.0016 0.0006 0.0006 0.0003 0.0001 0.0001 0.0013 0.0002 0.0027 0.0006 0.0001 0
Cell Periphery 0.055 0.0659 0.0465 0.0218 0.0428 0.0039 0.0377 0.0235 0.0319 0.0692 0.0041 0.0097 0.0152 0.0058 0.0119 0.0395 0.0005 0.0006
Cytoplasm 0.6072 0.7619 0.7166 0.7002 0.806 0.8605 0.6466 0.9296 0.834 0.7795 0.9201 0.8576 0.8474 0.9741 0.8927 0.7567 0.8587 0.9714
Cytoplasmic Foci 0.0003 0.0003 0.0004 0.0004 0.0005 0.0007 0.0323 0.0006 0.0091 0.0008 0.0014 0.0032 0.0043 0.0002 0.0043 0.0025 0.0587 0.0015
Eisosomes 0.0001 0.0004 0.0001 0 0 0 0.0008 0 0.0002 0 0 0.0001 0.0006 0 0.0001 0.0004 0.0002 0
Endoplasmic Reticulum 0.065 0.0175 0.021 0.0026 0.0052 0.0376 0.0398 0.0079 0.008 0.001 0.0022 0.0266 0.0123 0.0027 0.0048 0.0014 0.0007 0.0106
Endosome 0.0012 0.0005 0.0009 0.0015 0.0011 0.0019 0.029 0.0005 0.0018 0.0008 0.0022 0.0028 0.002 0.0002 0.0022 0.001 0.0016 0.0018
Golgi 0.0001 0.0001 0.0001 0 0 0.0001 0.0019 0.0002 0.0019 0.0001 0.0002 0.0007 0.0003 0 0.0008 0.0001 0.0002 0.0002
Lipid Particles 0.0016 0.0019 0.0016 0.0008 0.0013 0.0003 0.0102 0.0021 0.0051 0.0028 0.0009 0.0016 0.0023 0.0003 0.002 0.0027 0.0069 0.0003
Mitochondria 0.0019 0.0003 0.0002 0.0001 0.0002 0.0002 0.0098 0.0003 0.0031 0.0002 0.0001 0.0072 0.0076 0.0001 0.0038 0.0045 0.0032 0.0002
None 0.0011 0.0002 0.0003 0.0001 0.0001 0.0001 0.0153 0.0007 0.0027 0.0004 0.0004 0.0096 0.0084 0.0003 0.0033 0.0159 0.0192 0.0002
Nuclear Periphery 0.0559 0.0116 0.0181 0.0091 0.0115 0.0137 0.0333 0.0046 0.0065 0.0138 0.003 0.0108 0.0117 0.0033 0.0052 0.0094 0.0039 0.003
Nucleolus 0.0016 0.0005 0.0011 0.0005 0.0004 0.0001 0.0297 0.0003 0.0038 0.0024 0.0002 0.023 0.0109 0.0001 0.0025 0.0092 0.012 0
Nucleus 0.0112 0.0049 0.0067 0.0073 0.0068 0.0061 0.0148 0.002 0.0041 0.006 0.0023 0.0111 0.0119 0.0012 0.0033 0.0151 0.0039 0.0018
Peroxisomes 0 0 0 0 0 0 0.0013 0 0.001 0.0001 0 0.0001 0.0004 0 0.0008 0.0002 0.0007 0
Punctate Nuclear 0 0 0.0001 0 0 0 0.0021 0.0001 0.0003 0.0001 0.0001 0.0002 0.0025 0 0.0006 0.0006 0.007 0.0001
Vacuole 0.1753 0.1249 0.1648 0.225 0.1091 0.0617 0.068 0.0243 0.0708 0.1089 0.0543 0.0248 0.0442 0.0104 0.0375 0.1186 0.0164 0.0053
Vacuole Periphery 0.02 0.0057 0.0182 0.0283 0.011 0.0079 0.0105 0.001 0.0073 0.0112 0.006 0.0065 0.0126 0.0004 0.0038 0.0194 0.0054 0.0009

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 179.4121 178.912 161.3091 186.4966 214.3008 203.4587 251.6578 225.1808 206.7505 214.2114
Translational Efficiency 1.5403 1.587 1.4322 1.1878 1.2465 1.7459 1.3162 1.0771 1.0692 1.1025

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
117 1687 1587 436 249 1921 216 1692 366 3608 1803 2128

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 5875.59 9032.11 8105.12 5155.17 7153.70 8616.14 6253.53 5080.56 6745.12 8810.64 7883.30 5095.85
Standard Deviation 1436.06 2061.52 1447.35 1045.86 1696.29 2042.36 1034.11 1240.21 1723.98 2061.81 1527.57 1203.33
Intensity Change Log 2 0.620329 0.464100 -0.188714 0.268352 -0.194019 -0.493702 0.437767 0.140162 -0.348144

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000124 0.000076 0.000090 0.000255 0.000066 0.000047 0.000241 0.000239 0.000084 0.000060 0.000108 0.000242
Bud Neck 0.001642 0.000684 0.002134 0.002021 0.000486 0.000204 0.008113 0.001857 0.000855 0.000429 0.002851 0.001891
Bud Site 0.003743 0.002494 0.002014 0.009997 0.002455 0.001549 0.006168 0.008956 0.002867 0.001991 0.002511 0.009170
Cell Periphery 0.045670 0.008568 0.005321 0.033874 0.024699 0.005768 0.016752 0.009007 0.031402 0.007077 0.006691 0.014102
Cytoplasm 0.662292* 0.853011* 0.780227* 0.698159* 0.781032* 0.924358* 0.476037* 0.797973* 0.743074* 0.890998* 0.743785* 0.777522*
Cytoplasmic Foci 0.013847 0.006790 0.008827 0.005968 0.006710 0.003997 0.018469 0.004715 0.008992 0.005303 0.009983 0.004972
Eisosomes 0.000004 0.000001 0.000002 0.000005 0.000002 0.000001 0.000007 0.000003 0.000002 0.000001 0.000003 0.000003
Endoplasmic Reticulum 0.000321 0.000343 0.000144 0.000120 0.000442 0.000350 0.000161 0.000145 0.000404 0.000347 0.000146 0.000140
Endosome 0.000774 0.000384 0.000327 0.000295 0.000411 0.000175 0.001158 0.000773 0.000527 0.000273 0.000426 0.000675
Golgi 0.063426 0.024538 0.012694 0.008870 0.035787 0.011585 0.022733 0.008335 0.044622 0.017641 0.013897 0.008445
Lipid Particles 0.025322 0.008891 0.006421 0.028164 0.017015 0.005470 0.024297 0.008991 0.019671 0.007069 0.008563 0.012919
Mitochondria 0.000086 0.000157 0.000200 0.000065 0.000145 0.000146 0.000127 0.000653 0.000126 0.000151 0.000191 0.000533
Mitotic Spindle 0.000808 0.001757 0.001939 0.000995 0.000555 0.000956 0.002509 0.002380 0.000636 0.001331 0.002007 0.002096
None 0.002107 0.003320 0.002763 0.001635 0.002966 0.005283 0.001198 0.001888 0.002691 0.004365 0.002576 0.001836
Nuclear Periphery 0.001139 0.000628 0.001864 0.000368 0.000885 0.000196 0.001931 0.000253 0.000966 0.000398 0.001872 0.000277
Nuclear Periphery Foci 0.016701 0.011240 0.008154 0.005020 0.011756 0.004712 0.015835 0.002979 0.013337 0.007764 0.009074 0.003397
Nucleolus 0.000190 0.000054 0.000117 0.000145 0.000052 0.000045 0.000184 0.000246 0.000096 0.000049 0.000125 0.000225
Nucleus 0.021623 0.013874 0.050310 0.018877 0.012463 0.010164 0.041310 0.022292 0.015391 0.011899 0.049232 0.021592
Peroxisomes 0.000021 0.000012 0.000011 0.000090 0.000041 0.000015 0.000027 0.000082 0.000034 0.000014 0.000013 0.000084
Vacuole 0.100637 0.039094 0.096267 0.176951 0.067077 0.016754 0.336987* 0.116633 0.077806 0.027200 0.125105 0.128991
Vacuole Periphery 0.039522 0.024085 0.020174 0.008127 0.034956 0.008225 0.025755 0.011599 0.036416 0.015641 0.020842 0.010888

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.78 0.74 -2.14 -4.00 -3.23 1.56 -2.70 -4.17 -4.62 1.06 1.90 -3.07 -4.30 -5.19 -2.61
Bud Neck 1.39 -0.49 0.05 -5.68 1.94 2.99 -7.56 -5.76 -6.70 6.18 1.58 -6.19 -2.57 -7.18 4.80
Bud Site 1.44 1.86 -3.28 -4.53 -4.83 1.64 -3.67 -5.61 -6.86 -0.54 1.78 -0.27 -6.21 -7.58 -6.44
Cell Periphery 5.19 5.63 2.01 -6.69 -7.75 5.81 2.66 5.13 -2.44 3.28 7.56 7.66 5.77 -5.49 -5.85
Cytoplasm -6.18 -2.39 -0.69 10.64 3.15 -7.28 11.54 0.32 20.25 -14.47 -8.69 2.45 -0.79 19.36 -6.18
Cytoplasmic Foci 3.58 1.18 3.48 0.23 4.20 2.36 -5.71 1.25 -2.48 6.46 3.55 -3.53 3.44 -0.08 9.18
Eisosomes 1.85 1.21 0.00 -5.93 -3.98 3.87 -5.37 -3.14 -6.62 4.43 2.58 -1.23 -0.92 -7.28 0.51
Endoplasmic Reticulum -0.26 1.85 2.21 3.70 1.21 0.50 2.91 3.25 1.23 0.96 0.50 3.68 3.81 2.24 0.43
Endosome 1.72 1.75 2.23 1.22 2.08 3.68 -6.40 -2.95 -5.31 3.11 2.92 0.12 -1.38 -4.25 -1.83
Golgi 3.91 5.17 5.69 9.80 3.92 5.76 3.81 6.76 4.28 6.88 6.26 7.23 8.71 11.21 7.77
Lipid Particles 3.71 4.05 -0.45 -7.04 -7.72 3.35 -2.00 2.33 -3.65 5.86 4.58 3.54 2.47 -6.07 -2.87
Mitochondria -1.03 -2.71 0.97 3.88 6.86 -0.20 -1.01 -1.85 -1.83 -1.69 -0.86 -3.47 -1.82 -1.71 -1.29
Mitotic Spindle -2.06 -4.80 -0.23 1.58 3.21 -1.37 -3.94 -2.93 -2.06 0.14 -2.62 -6.42 -2.87 -1.24 0.52
None -1.70 -1.67 0.84 2.02 1.93 -2.19 2.71 1.36 3.48 -1.38 -2.38 0.33 1.45 3.73 1.49
Nuclear Periphery 1.56 -2.43 2.47 4.36 5.22 4.85 -3.42 4.01 -1.91 5.50 3.96 -3.73 4.63 2.26 6.00
Nuclear Periphery Foci 1.51 2.24 3.63 7.13 5.98 4.32 -1.33 5.37 3.08 6.73 3.49 2.12 6.53 8.61 10.44
Nucleolus 1.47 0.61 0.63 -3.72 0.11 0.25 -8.86 -4.85 -4.84 -0.90 1.44 -1.63 -2.98 -5.21 -2.45
Nucleus 1.73 -6.13 -0.07 -4.03 10.63 0.70 -7.13 -4.91 -10.38 5.14 1.41 -12.78 -3.87 -10.49 12.48
Peroxisomes 1.31 1.29 -3.10 -4.70 -4.73 2.21 -0.08 -2.52 -5.33 -2.75 2.62 2.40 -3.53 -6.60 -6.45
Vacuole 4.28 -1.12 -4.78 -12.89 -5.66 5.65 -12.45 -6.58 -22.89 10.00 6.56 -7.36 -7.49 -24.53 0.31
Vacuole Periphery 1.50 2.00 3.26 8.42 7.09 5.30 2.58 4.87 -2.06 3.93 4.41 3.53 5.72 5.42 7.26