TheCellVision

TPS1

Standard name	TPS1 ORF YBR126C Aliases alpha,alpha-trehalose-phosphate synthase (UDP-forming) TPS1, BYP1, CIF1, FDP1, GGS1, GLC6, TSS1
Human Ortholog
Description	Synthase subunit of trehalose-6-P synthase/phosphatase complex; synthesizes the storage carbohydrate trehalose, which is critically important for survival of long-term desiccation; also found in a monomeric form; expression is induced by the stress response and repressed by the Ras-cAMP pathway; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid

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Scale images

tps1-supp1

Micrographs

Sub-cellular Localization

LOC-score
Cell count

	ensLOC																		DeepLoc
Localization	WT1	WT2	WT3	RAP60	RAP140	RAP220	RAP300	RAP380	RAP460	RAP540	RAP620	RAP700	HU80	HU120	HU160	rpd3Δ_1	rpd3Δ_2	rpd3Δ_3	WT1	WT2	WT3	AF100	AF140	AF180
Cortical Patches	0	0	0	0	0	0	0	0	0	0	0.06	0	0	0	0	0	0	0	0	0	0	0	0	0
Bud	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Bud Neck	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Bud Site	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	–	–	–	–	–	–
Cell Periphery	0	0	0	0	0	0	0	0	0.06	0.06	0.07	0.08	0	0	0	0	0.05	0	0	0	0	0	0	0
Cytoplasm	0.99	0.98	0.98	0.98	0.92	0.95	0.82	0.77	0.79	0.76	0.72	0.71	0.99	0.99	0.99	0.93	0.93	0.96	0.95	0.96	0.88	0.93	0.91	0.92
Endoplasmic Reticulum	0	0	0	0	0.05	0	0.13	0.17	0.12	0.12	0.11	0.13	0	0	0	0	0	0	0	0	0.05	0	0	0
Endosome	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Golgi	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Mitochondria	0	0	0	0	0	0	0	0	0	0	0.07	0.08	0	0	0	0	0	0	0	0	0	0	0	0
Nucleus	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Nuclear Periphery	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Nucleolus	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Peroxisomes	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
SpindlePole	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Vac/Vac Membrane	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0.05	0	0	0	0	0	0	0

	ensLOC																		DeepLoc
Localization	WT1	WT2	WT3	RAP60	RAP140	RAP220	RAP300	RAP380	RAP460	RAP540	RAP620	RAP700	HU80	HU120	HU160	rpd3Δ_1	rpd3Δ_2	rpd3Δ_3	WT1	WT2	WT3	AF100	AF140	AF180
Cortical Patches	0	0	0	0	0	2	4	5	9	6	11	9	0	0	0	0	0	0	0	2	1	0	3	0
Bud	0	0	0	0	0	0	2	1	1	0	2	1	0	0	1	1	0	2	0	0	1	0	0	0
Bud Neck	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Bud Site	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	–	–	–	–	–	–
Cell Periphery	2	9	7	3	8	11	7	13	15	14	13	18	3	2	6	8	14	12	0	0	1	0	0	0
Cytoplasm	232	254	260	126	197	246	220	234	202	164	125	169	197	284	291	230	260	269	230	247	241	153	217	144
Endoplasmic Reticulum	2	4	4	0	10	7	36	53	31	26	20	31	2	3	5	8	12	7	1	2	13	3	7	4
Endosome	0	0	1	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	1	0	0	1
Golgi	0	0	0	0	1	1	1	3	6	4	2	7	0	0	0	3	5	0	0	0	3	0	1	1
Mitochondria	1	0	0	1	1	3	1	4	8	3	13	18	0	1	0	4	2	0	0	0	1	0	0	0
Nucleus	0	0	0	0	0	0	1	1	0	0	0	0	0	0	0	0	1	0	1	0	0	1	0	0
Nuclear Periphery	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	2	0	0	0	0	1
Nucleolus	0	0	0	0	0	0	1	0	1	1	1	2	0	0	0	0	1	0	0	0	0	0	0	0
Peroxisomes	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
SpindlePole	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Vac/Vac Membrane	0	0	1	0	4	0	2	10	3	9	4	6	1	0	0	9	15	3	2	1	1	1	1	0
Unique Cell Count	234	259	265	128	214	259	268	305	255	216	174	238	199	287	295	246	281	279	242	258	273	166	238	156
Labelled Cell Count	237	267	273	130	221	270	276	324	276	227	191	261	203	290	304	263	310	293	242	258	273	166	238	156

Yeast GFP Assignment

Cytoplasm

(Huh et al. Nature 2003 425:686)

Protein Abundance

Screen	WT1	WT2	WT3	RAP60	RAP140	RAP220	RAP300	RAP380	RAP460	RAP540	RAP620	RAP700	HU80	HU120	HU160	rpd3Δ_1	rpd3Δ_2	rpd3Δ_3	AF100	AF140	AF180
Mean Cell GFP Intensity (1e^-4)	20.9	36.4	30.9	31.0	36.7	37.4	39.5	38.7	41.0	42.8	40.4	44.8	41.8	59.6	64.8	35.6	41.7	45.1	22.4	24.2	28.3
Std Deviation (1e^-4)	4.5	8.4	11.0	11.4	11.3	9.7	12.2	12.2	13.6	16.9	16.6	19.6	13.1	21.9	27.8	13.4	13.0	13.5	6.6	5.7	8.1
Intensity Change (Log₂)	–	–	–	0.0	0.25	0.27	0.35	0.32	0.41	0.47	0.39	0.54	0.44	0.95	1.07	0.2	0.43	0.54	-0.47	-0.35	-0.13

Localization Change

Localization	RAP60	RAP140	RAP220	RAP300	RAP380	RAP460	RAP540	RAP620	RAP700	HU80	HU120	HU160	rpd3Δ_1	rpd3Δ_2	rpd3Δ_3
Cortical Patches	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Bud	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Bud Neck	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Bud Site	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Cell Periphery	0	0.7	1.0	0	1.0	1.8	2.1	2.4	2.5	0	0	-0.5	0.4	1.4	1.1
Cytoplasm	0.2	-3.1	-2.0	-6.2	-7.5	-6.8	-7.5	-8.2	-8.6	0.8	0.8	0.5	-2.6	-3.1	-1.2
Endoplasmic Reticulum	0	2.0	1.0	5.2	6.3	4.8	4.7	4.5	5.1	0	0	0	1.3	1.9	0.8
Endosome	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Golgi	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Mitochondria	0	0	0	0	0	0	0	4.5	4.6	0	0	0	0	0	0
Nucleus	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Nuclear Periphery	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Nucleolus	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Peroxisomes	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
SpindlePole	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Vacuole	0	0	0	0	2.5	0	0	0	0	0	0	0	0	3.4	0

External localization resources

dHITS | Loqate | YeastGFP | YeastRGB | YeastRC

Images

Protein Concentration and Protein Localization Data

	R1						R2						R3
	G1 Pre-START	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase	G1 Pre-START	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase	G1 Pre-START	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase
Concentration	33.8124	44.4769	39.5281	36.5198	37.4122	41.5602	32.2716	43.6869	39.4567	40.2931	21.3442	37.6519	48.0105	51.2604	46.3155	40.9043	38.1426	48.1277
Actin	0.027	0.0001	0.0064	0.0107	0.0054	0.0011	0.0127	0.0001	0.0006	0	0.0206	0.0091	0.0075	0.0038	0.0007	0.0038	0.0118	0.0003
Bud	0.0015	0.0001	0.0003	0.0011	0.0021	0.0003	0.0011	0	0.0015	0.0001	0.0004	0.0012	0.0001	0.0002	0.0004	0.0012	0.0053	0.0001
Bud Neck	0.0023	0.0002	0.0004	0.0007	0.0008	0.0015	0.0009	0.0001	0.0003	0.0002	0.0006	0.0008	0.0003	0.0001	0.0003	0.0005	0.0005	0.0004
Bud Periphery	0.0017	0.0001	0.0004	0.0011	0.0019	0.0002	0.0022	0	0.0004	0	0.0004	0.001	0.0001	0.0002	0.0004	0.0011	0.0033	0
Bud Site	0.0026	0.0001	0.0006	0.0004	0.0075	0.0001	0.0019	0	0.0011	0	0.0011	0.0009	0.0002	0.0003	0.0003	0.0015	0.0125	0
Cell Periphery	0.0007	0.0002	0.0008	0.0007	0.0009	0.0003	0.0011	0	0.0004	0	0.0001	0.0001	0.0001	0	0.0004	0.0002	0.0002	0
Cytoplasm	0.7892	0.9864	0.9043	0.9034	0.805	0.9004	0.7904	0.9979	0.9239	0.9919	0.5911	0.9312	0.9332	0.9647	0.9289	0.8985	0.8619	0.987
Cytoplasmic Foci	0.0299	0.0028	0.0137	0.0078	0.0244	0.0087	0.0146	0.0004	0.0183	0.0015	0.079	0.0069	0.0039	0.0036	0.0078	0.0155	0.0262	0.0016
Eisosomes	0.0006	0	0.0002	0.0003	0.0008	0	0.0003	0	0	0	0.0001	0	0	0.0001	0.0002	0.0001	0.0001	0
Endoplasmic Reticulum	0.009	0.0017	0.0057	0.0086	0.0041	0.0082	0.0262	0.0002	0.0023	0.0004	0.0191	0.0016	0.006	0.0004	0.0082	0.0073	0.0041	0.006
Endosome	0.014	0.0008	0.0073	0.0053	0.0215	0.0155	0.0272	0.0002	0.0124	0.0017	0.0999	0.0058	0.0171	0.0009	0.0065	0.0062	0.009	0.0012
Golgi	0.003	0	0.0036	0.0008	0.0073	0.0021	0.0099	0	0.0017	0	0.0129	0.0046	0.0054	0.0018	0.0005	0.002	0.0044	0.0001
Lipid Particles	0.0073	0.0001	0.0073	0.0023	0.0399	0.0026	0.004	0	0.0009	0	0.039	0.0021	0.0025	0.0096	0.0034	0.0013	0.0065	0
Mitochondria	0.0108	0	0.0082	0.0013	0.0403	0.0184	0.0277	0	0.0198	0.0001	0.0035	0.0038	0.0037	0.0111	0.0082	0.049	0.0194	0.0001
None	0.0106	0.0003	0.0029	0.0104	0.0034	0.0005	0.0112	0.0001	0.0043	0.0003	0.0083	0.0003	0.0008	0.0003	0.0082	0.0019	0.0075	0.0002
Nuclear Periphery	0.0165	0.0011	0.0065	0.0053	0.0034	0.0054	0.032	0.0002	0.001	0.0008	0.0746	0.0013	0.0049	0.0004	0.0041	0.0011	0.0024	0.0009
Nucleolus	0.0066	0	0.0011	0.0002	0.0056	0.004	0.0015	0	0.0003	0	0.0016	0.0211	0.0001	0	0.0024	0.0002	0.0008	0
Nucleus	0.0142	0.0014	0.007	0.0053	0.0055	0.0046	0.0128	0.0004	0.0016	0.0014	0.0094	0.0037	0.0047	0.0007	0.0038	0.0012	0.0034	0.0012
Peroxisomes	0.0026	0	0.0015	0.0022	0.003	0.0012	0.0008	0	0.0005	0	0.0083	0.0012	0.0008	0.0014	0.0019	0.0028	0.0094	0
Punctate Nuclear	0.0197	0.0001	0.0017	0.0031	0.0008	0.0007	0.0045	0	0.0004	0	0.0199	0.0015	0.0002	0.0001	0.0007	0.0001	0.0062	0.0001
Vacuole	0.0223	0.004	0.0112	0.0213	0.0101	0.0163	0.0135	0.0003	0.0065	0.0012	0.0054	0.0012	0.007	0.0003	0.0089	0.0029	0.0037	0.0005
Vacuole Periphery	0.0081	0.0005	0.009	0.0077	0.0063	0.008	0.0035	0.0001	0.0016	0.0003	0.0046	0.0005	0.0013	0.0002	0.0036	0.0013	0.0013	0.0002

Sequencing Data

	R1					R2
	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase
Gene Expression	66.7382	45.7392	80.1782	140.3026	85.5675	131.1791	86.9642	128.4731	197.0533	106.8383
Translational Efficiency	2.2837	2.2216	1.9841	2.104	2.2195	1.9921	1.9793	1.9436	1.7251	1.7764

Hit Data

Dataset	Hit
Protein Concentration	✘
Protein Localization	✘
Gene Expression	✔
Translational Efficiency	✘

Proteome Dynamics

Micrographs

Cell Count

R1				R2				R1 & R2
WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14
140	1203	587	1519	2495	1967	2730	1133	2635	3170	3317	2652

Protein Abundance

	R1				R2				R1 & R2
	WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14
Mean	1791.10	1613.53	5228.98	4132.07	1687.55	1690.05	4163.73	4139.46	1693.05	1661.01	4352.24	4135.23
Standard Deviation	415.38	371.12	1174.31	1209.88	333.01	343.70	992.84	1187.87	338.69	356.30	1104.81	1200.53
Intensity Change Log ₂	—	-0.150625	1.545684	1.206019	—	0.002136	1.302946	1.294512	—	-0.074497	1.433014	1.249627

Localization Score (DeepLoc)

	R1				R2				R1 & R2
	WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14
Actin	0.000279	0.000274	0.000136	0.000278	0.000042	0.000164	0.000025	0.000275	0.000055	0.000206	0.000045	0.000276
Bud Neck	0.000504	0.000534	0.002845	0.001454	0.000201	0.000393	0.000570	0.002231	0.000217	0.000446	0.000973	0.001786
Bud Site	0.020984	0.009767	0.004348	0.010784	0.004882	0.009165	0.001719	0.010258	0.005738	0.009394	0.002184	0.010559
Cell Periphery	0.003035	0.000342	0.015180	0.006339	0.000155	0.000178	0.000637	0.013264	0.000308	0.000240	0.003211	0.009298
Cytoplasm	0.711699*	0.925067*	0.730407*	0.839762*	0.946581*	0.919945*	0.903733*	0.761055*	0.934101*	0.921889*	0.873060*	0.806136*
Cytoplasmic Foci	0.083105	0.020160	0.027001	0.006378	0.021874	0.027238	0.004234	0.008723	0.025127	0.024552	0.008263	0.007380
Eisosomes	0.000002	0.000002	0.000003	0.000003	0.000001	0.000001	0.000000	0.000004	0.000001	0.000001	0.000001	0.000003
Endoplasmic Reticulum	0.006936	0.000835	0.000159	0.000191	0.000587	0.000611	0.000081	0.000133	0.000924	0.000696	0.000095	0.000166
Endosome	0.006870	0.002116	0.000816	0.000928	0.000760	0.002246	0.000347	0.001254	0.001085	0.002197	0.000430	0.001067
Golgi	0.101456	0.008308	0.035104	0.007230	0.007139	0.010146	0.003279	0.010960	0.012150	0.009449	0.008911	0.008824
Lipid Particles	0.003701	0.001843	0.012878	0.010299	0.000517	0.000764	0.001047	0.020308	0.000686	0.001173	0.003141	0.014575
Mitochondria	0.000031	0.000602	0.000137	0.000145	0.000123	0.000367	0.000103	0.000233	0.000118	0.000456	0.000109	0.000182
Mitotic Spindle	0.003028	0.001424	0.004041	0.001815	0.001120	0.005330	0.001631	0.001127	0.001221	0.003848	0.002057	0.001521
None	0.000584	0.001955	0.002677	0.002008	0.001593	0.000963	0.001675	0.002023	0.001539	0.001339	0.001853	0.002015
Nuclear Periphery	0.000046	0.000040	0.001307	0.000133	0.000016	0.000040	0.000146	0.000218	0.000017	0.000040	0.000351	0.000169
Nuclear Periphery Foci	0.004732	0.002829	0.013261	0.003144	0.000824	0.001481	0.001807	0.004695	0.001031	0.001993	0.003834	0.003807
Nucleolus	0.000021	0.000204	0.000118	0.000134	0.000027	0.000040	0.000112	0.000182	0.000027	0.000102	0.000113	0.000155
Nucleus	0.002581	0.006784	0.041382	0.020464	0.003940	0.004455	0.025669	0.019165	0.003868	0.005339	0.028450	0.019909
Peroxisomes	0.000100	0.000214	0.000022	0.000108	0.000062	0.000128	0.000033	0.000077	0.000064	0.000161	0.000031	0.000095
Vacuole	0.016661	0.012561	0.084954	0.082710	0.004673	0.011279	0.045743	0.135707	0.005310	0.011766	0.052682	0.105352
Vacuole Periphery	0.033642	0.004137	0.023226	0.005693	0.004884	0.005066	0.007407	0.008110	0.006412	0.004713	0.010206	0.006726

Localization Changes (T-score)

	R1					R2					R1 & R2
	UBP2_WT	UBP14_WT	UBP2UBP14_WT	UBP2UBP14_UBP2	UBP2UBP14_UBP14	UBP2_WT	UBP14_WT	UBP2UBP14_WT	UBP2UBP14_UBP2	UBP2UBP14_UBP14	UBP2_WT	UBP14_WT	UBP2UBP14_WT	UBP2UBP14_UBP2	UBP2UBP14_UBP14
Actin	-0.01	1.79	-0.36	-0.37	-1.93	-4.36	1.17	-4.59	-1.89	-4.68	-5.78	-1.06	-5.08	-1.62	-4.98
Bud Neck	-0.44	-7.95	-7.24	-7.71	4.29	-3.89	-8.22	-8.07	-7.37	-5.41	-5.37	-11.67	-12.23	-10.66	-4.09
Bud Site	2.94	4.39	2.35	-1.21	-5.10	-5.67	7.46	-4.42	-0.80	-6.81	-5.61	7.78	-5.52	-1.57	-9.29
Cell Periphery	4.25	-6.95	-4.12	-12.44	5.24	-1.22	-5.76	-11.56	-11.53	-11.02	1.60	-9.25	-16.23	-16.39	-8.99
Cytoplasm	-7.62	0.29	-3.67	14.61	-8.23	6.56	16.57	24.89	20.43	16.60	3.47	18.52	26.68	24.26	11.11
Cytoplasmic Foci	5.86	4.75	7.10	8.42	10.54	-3.09	15.40	9.09	10.72	-5.80	0.27	12.10	12.94	14.11	1.63
Eisosomes	0.52	-2.40	-1.15	-1.89	1.41	-6.15	0.54	-7.04	-5.75	-7.10	-4.78	-5.57	-9.21	-5.79	-7.65
Endoplasmic Reticulum	2.02	2.25	2.24	3.60	-0.67	-0.14	4.55	4.17	4.11	-1.87	1.07	4.29	3.91	5.23	-2.21
Endosome	3.75	4.87	4.77	3.03	-0.11	-6.38	4.19	-1.38	3.12	-2.64	-5.40	5.58	0.29	4.62	-2.83
Golgi	6.50	4.61	6.59	1.32	12.27	-2.93	6.45	-2.87	0.19	-7.78	2.33	2.88	3.57	1.54	1.14
Lipid Particles	2.01	-6.79	-6.01	-9.30	2.12	-2.28	-5.00	-11.81	-11.63	-11.23	-2.98	-10.09	-16.08	-15.31	-12.00
Mitochondria	-1.61	-10.30	-3.17	1.17	-0.49	-1.85	0.26	-1.69	0.91	-1.74	-2.14	-0.83	-2.15	1.55	-1.96
Mitotic Spindle	1.36	-0.83	1.01	-0.54	1.85	-5.01	-2.75	-0.38	4.64	1.95	-4.80	-3.33	-0.91	3.76	2.03
None	-3.75	-9.19	-3.49	0.01	1.74	2.08	-0.20	-0.45	-1.76	-0.38	0.66	-0.96	-0.88	-1.74	-0.22
Nuclear Periphery	0.30	-11.11	-5.42	-6.39	10.14	-3.03	-11.39	-13.11	-10.59	-1.54	-3.81	-14.84	-14.26	-11.34	7.65
Nuclear Periphery Foci	1.19	-5.17	0.91	-0.91	8.42	-2.66	-5.85	-12.02	-8.30	-6.84	-4.50	-10.43	-12.97	-6.74	0.78
Nucleolus	-2.12	-11.30	-6.93	0.77	-0.38	-3.55	-17.77	-3.86	-3.55	-1.40	-2.29	-20.35	-6.50	-1.48	-1.38
Nucleus	-5.34	-14.73	-14.03	-12.15	6.66	-1.54	-22.93	-14.49	-14.15	4.94	-4.56	-26.91	-19.73	-18.48	6.90
Peroxisomes	-2.25	3.63	-0.14	2.27	-5.04	-5.31	4.22	-1.09	3.55	-3.63	-4.87	5.19	-2.48	3.29	-5.76
Vacuole	0.86	-10.61	-13.37	-17.68	-0.29	-6.43	-20.88	-23.44	-22.15	-14.67	-7.93	-24.61	-30.11	-28.00	-13.39
Vacuole Periphery	3.64	1.35	3.49	-1.47	7.80	-0.20	-3.19	-2.59	-2.28	-0.10	2.11	-3.96	0.30	-2.35	5.27