Standard name
Human Ortholog
Description Protein of unknown function; overproduction blocks cell cycle arrest in the presence of mating pheromone; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0.05 0 0 0.05
Bud 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.14 0.12 0.07 0.09 0.11 0 0.06 0.06 0.06 0.05 0.07 0.24 0.34 0.34 0.44 0.59 0.41 0.15 0.1 0.23 0.16 0.18 0.22
Cytoplasm 0.96 0.96 1.0 0.99 0.96 0.93 0.94 0.87 0.83 0.79 0.82 0.98 0.98 0.99 0.86 0.68 0.81 0.73 0.76 0.37 0.72 0.71 0.64
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.07 0.15 0.1 0.24 0.25 0.38 0.3 0 0 0 0 0 0 0 0 0.12 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0.06 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 1 0 0 1 1 3 1 4 2 5 1 0 4 14 4 2 10 12 21
Bud 3 4 1 3 0 3 2 12 12 10 7 13 4 2 2 0 1 0 0 0 0 2 6
Bud Neck 0 0 1 3 0 1 1 1 3 1 0 5 1 2 0 0 0 5 2 1 7 11 16
Bud Site 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 35 12 9 20 23 12 19 15 17 11 16 71 156 176 57 13 44 38 10 9 43 79 105
Cytoplasm 248 96 121 227 200 285 313 207 241 181 183 294 446 514 112 15 87 189 77 15 194 304 303
Endoplasmic Reticulum 2 1 0 0 0 0 0 3 1 0 0 2 1 0 5 0 5 3 2 0 4 3 4
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 1 1 0 0 0 1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Mitochondria 1 1 1 1 15 47 33 57 72 86 68 0 0 0 1 0 1 2 1 5 3 4 4
Nucleus 0 0 2 3 2 0 6 2 3 0 1 0 0 1 0 0 0 0 0 0 0 0 0
Nuclear Periphery 1 2 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 1 0 0 0 1 1 0 0 0 2 0 0 1 0 0 0 0 0 1
Vac/Vac Membrane 0 0 0 0 1 5 0 0 3 0 0 0 0 0 0 0 7 0 0 2 1 0 1
Unique Cell Count 257 100 121 230 208 305 332 237 291 228 223 301 456 520 130 22 107 261 102 41 271 431 474
Labelled Cell Count 290 117 135 258 244 353 374 298 355 294 276 389 610 703 180 28 150 261 102 41 271 431 474


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.0 6.0 5.7 6.0 4.9 4.8 5.4 4.1 4.4 4.0 4.1 6.2 6.4 6.5 7.6 8.5 8.4 5.8 6.3 7.0
Std Deviation (1e-4) 0.6 0.7 1.4 1.6 1.2 1.7 1.8 0.9 1.5 0.9 0.7 0.7 0.8 1.3 1.4 1.2 2.4 1.0 1.2 1.6
Intensity Change (Log2)


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.909 0.9673 0.5584 0.7267 0.3694 0.7261 0.889 1.6455 1.514 0.9594 1.3318 1.1017 0.9081 1.1171 1.1438 1.4981 1.4817 0.814
Actin 0.0588 0.005 0.011 0.0101 0.0471 0.025 0.0326 0.0022 0.0144 0.0033 0.0247 0.0023 0.0172 0.0053 0.0203 0.0742 0.0276 0.0048
Bud 0.0008 0.001 0.0014 0.0118 0.0004 0.002 0.0006 0.0026 0.0008 0.0303 0.0015 0.0002 0.0004 0.0005 0.0003 0.0021 0.0005 0.0003
Bud Neck 0.0082 0.0046 0.005 0.0113 0.0026 0.0074 0.0016 0.0021 0.0046 0.0052 0.0211 0.0069 0.0046 0.002 0.003 0.0031 0.0026 0.0082
Bud Periphery 0.0017 0.0012 0.0022 0.0268 0.0015 0.0053 0.0013 0.0063 0.0017 0.0127 0.0042 0.0008 0.0007 0.001 0.001 0.0082 0.0035 0.0011
Bud Site 0.005 0.0452 0.0105 0.0126 0.0012 0.0023 0.0061 0.0348 0.0343 0.0318 0.0065 0.0004 0.0127 0.0527 0.0057 0.006 0.0015 0.0007
Cell Periphery 0.0131 0.0113 0.0177 0.011 0.0081 0.0084 0.0171 0.0194 0.0225 0.0151 0.0151 0.0143 0.0186 0.0228 0.0198 0.015 0.0377 0.0115
Cytoplasm 0.5505 0.6587 0.643 0.675 0.6951 0.6667 0.5806 0.6936 0.7206 0.6876 0.6739 0.8772 0.7159 0.7686 0.8054 0.6711 0.716 0.8424
Cytoplasmic Foci 0.0258 0.008 0.0085 0.0109 0.006 0.0093 0.0208 0.0064 0.0038 0.0074 0.0098 0.003 0.0158 0.005 0.0055 0.0123 0.0031 0.0035
Eisosomes 0.001 0.0011 0.0013 0.0005 0.0008 0.0009 0.0024 0.0005 0.0003 0.0002 0.0002 0.0001 0.0008 0.0013 0.0009 0.0012 0.0014 0.0004
Endoplasmic Reticulum 0.0076 0.0046 0.0057 0.005 0.0063 0.0063 0.0032 0.002 0.002 0.0032 0.0014 0.0018 0.0055 0.0042 0.0057 0.0054 0.0099 0.0034
Endosome 0.0174 0.0009 0.0042 0.007 0.0046 0.0082 0.0137 0.002 0.0033 0.0031 0.0376 0.0002 0.0075 0.0003 0.0023 0.0037 0.0195 0.0008
Golgi 0.0072 0.0007 0.0034 0.0023 0.0048 0.0044 0.0076 0.0008 0.0031 0.0038 0.0095 0.0002 0.0055 0.0006 0.002 0.0072 0.0046 0.0006
Lipid Particles 0.0098 0.0008 0.0012 0.0013 0.0021 0.002 0.0107 0.0012 0.0014 0.012 0.037 0.0003 0.0086 0.0007 0.0029 0.011 0.0019 0.0005
Mitochondria 0.0019 0.0003 0.0074 0.0016 0.032 0.0058 0.0162 0.0004 0.0017 0.0161 0.0312 0.0001 0.0053 0.0002 0.0019 0.0035 0.0726 0.0001
None 0.2648 0.2515 0.2703 0.2052 0.1813 0.2303 0.2576 0.2194 0.1826 0.1526 0.0784 0.0907 0.1512 0.1331 0.1163 0.132 0.0812 0.1204
Nuclear Periphery 0.0049 0.0004 0.0008 0.0005 0.0017 0.0054 0.0006 0.0003 0.0002 0.0006 0.0007 0.0001 0.0078 0.0002 0.0007 0.0009 0.0105 0.0001
Nucleolus 0.0008 0.0004 0.0015 0.0005 0.0002 0.0003 0.0002 0.0008 0.0001 0.0012 0.0032 0 0.0004 0.0001 0.0002 0.0002 0.0003 0
Nucleus 0.0022 0.0014 0.0015 0.0011 0.0006 0.0041 0.0005 0.0011 0.0005 0.0038 0.002 0.0003 0.0041 0.0004 0.0008 0.0006 0.0018 0.0003
Peroxisomes 0.0066 0.0004 0.0009 0.0018 0.0009 0.0032 0.0198 0.0007 0.0008 0.0061 0.0212 0 0.0142 0.0001 0.0026 0.0404 0.0004 0
Punctate Nuclear 0.009 0.0012 0.0009 0.0008 0.0005 0.0009 0.0036 0.0023 0.0003 0.0009 0.0007 0.0007 0.0009 0.0002 0.0015 0.001 0.0022 0.0001
Vacuole 0.0024 0.0015 0.0014 0.0025 0.0014 0.0012 0.0022 0.001 0.0009 0.0026 0.0149 0.0003 0.002 0.0006 0.001 0.0009 0.0008 0.0006
Vacuole Periphery 0.0005 0.0001 0.0003 0.0004 0.0007 0.0004 0.001 0.0001 0.0001 0.0005 0.0051 0 0.0004 0 0.0001 0.0002 0.0006 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 14.5332 13.9912 10.382 19.7861 21.0171 8.9385 16.1674 15.3448 19.0457 22.3476
Translational Efficiency 0.8562 0.727 1.0905 0.9291 0.5967 1.4074 0.6702 1.2128 0.8462 0.737

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2081 361 517 135 2056 1474 221 1101 4137 1835 738 1236

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 792.83 889.65 943.98 949.19 775.52 785.89 1031.06 902.64 784.23 806.30 970.06 907.72
Standard Deviation 102.76 116.04 129.95 105.77 102.51 120.18 118.94 114.67 103.00 126.30 132.88 114.65
Intensity Change Log 2 0.166226 0.251745 0.259685 0.019163 0.410892 0.218987 0.095379 0.332634 0.239704

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.002630 0.012180 0.007805 0.027440 0.002075 0.004570 0.002822 0.004290 0.002354 0.006067 0.006313 0.006819
Bud Neck 0.025092 0.037453 0.011547 0.010272 0.017538 0.034285 0.008197 0.008495 0.021338 0.034908 0.010544 0.008689
Bud Site 0.017438 0.052259 0.048573 0.093553 0.019334 0.035003 0.026636 0.047963 0.018380 0.038397 0.042004 0.052943
Cell Periphery 0.002118 0.003680 0.003644 0.012993 0.001825 0.002618 0.003136 0.001747 0.001972 0.002827 0.003492 0.002975
Cytoplasm 0.544126* 0.460112* 0.374198* 0.241562* 0.569966* 0.548775* 0.286492* 0.472840* 0.556968* 0.531333* 0.347934* 0.447579*
Cytoplasmic Foci 0.154801 0.196178* 0.055541 0.060051 0.126021 0.161309 0.072728 0.056635 0.140498 0.168169 0.060688 0.057008
Eisosomes 0.005811 0.004305 0.000714 0.004945 0.003460 0.005332 0.000789 0.001149 0.004643 0.005130 0.000737 0.001563
Endoplasmic Reticulum 0.002567 0.007694 0.023118 0.030548 0.002325 0.001869 0.015244 0.001660 0.002446 0.003015 0.020760 0.004815
Endosome 0.007897 0.013440 0.004616 0.004364 0.004822 0.012335 0.008679 0.007881 0.006369 0.012553 0.005832 0.007497
Golgi 0.008423 0.010598 0.000345 0.000568 0.005431 0.009031 0.000235 0.005613 0.006936 0.009340 0.000312 0.005062
Lipid Particles 0.008795 0.008532 0.004819 0.006468 0.005215 0.006290 0.008630 0.001869 0.007016 0.006731 0.005960 0.002372
Mitochondria 0.003454 0.006336 0.000454 0.000190 0.002179 0.003607 0.000105 0.001098 0.002820 0.004144 0.000350 0.000999
Mitotic Spindle 0.000231 0.001972 0.001839 0.000722 0.001042 0.001234 0.000159 0.014972 0.000634 0.001380 0.001336 0.013416
None 0.003149 0.000509 0.001043 0.001639 0.004722 0.001877 0.000631 0.001491 0.003931 0.001608 0.000920 0.001507
Nuclear Periphery 0.000116 0.000095 0.000739 0.000246 0.000104 0.000101 0.000849 0.000314 0.000110 0.000100 0.000772 0.000306
Nuclear Periphery Foci 0.000749 0.001843 0.008664 0.005685 0.000870 0.000811 0.010979 0.001483 0.000809 0.001014 0.009357 0.001942
Nucleolus 0.000446 0.000143 0.000146 0.000047 0.000383 0.000445 0.000190 0.000084 0.000415 0.000386 0.000159 0.000080
Nucleus 0.146790* 0.063605 0.261654* 0.067154 0.173986* 0.084570 0.164673* 0.079916 0.160306* 0.080445 0.232612* 0.078522
Peroxisomes 0.004748 0.008342 0.000658 0.001958 0.002709 0.006818 0.000698 0.001886 0.003735 0.007118 0.000670 0.001894
Vacuole 0.060091 0.109737 0.189693 0.429529* 0.055452 0.078256 0.388003* 0.284862* 0.057785 0.084449 0.249079* 0.300663*
Vacuole Periphery 0.000530 0.000987 0.000189 0.000066 0.000541 0.000863 0.000126 0.003752 0.000535 0.000887 0.000170 0.003349

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.00 -3.47 -3.98 -1.46 -2.78 -5.02 -1.27 -1.54 1.67 -0.70 -5.00 -3.72 -3.31 0.97 0.73
Bud Neck -2.54 9.11 11.29 5.96 2.37 -8.30 6.95 11.02 14.04 1.11 -7.17 10.52 16.65 15.15 4.19
Bud Site -6.59 -7.68 -5.39 -1.91 -2.47 -6.26 -1.88 -5.03 -0.67 -2.53 -9.23 -7.64 -6.93 -0.87 -0.24
Cell Periphery -3.15 -3.81 -4.27 -3.26 -3.53 -3.39 -3.27 2.95 5.15 4.84 -4.11 -4.99 -0.89 2.01 2.88
Cytoplasm 6.24 17.70 15.11 9.45 4.52 2.85 19.71 15.33 11.96 -9.49 4.04 25.77 17.84 13.09 -7.39
Cytoplasmic Foci -4.51 22.33 15.92 14.32 -0.25 -6.77 10.99 19.81 23.29 2.68 -6.23 24.51 28.83 27.28 1.21
Eisosomes 2.25 15.03 2.93 0.68 -4.64 -2.61 10.53 9.23 6.60 -0.95 -0.80 17.96 14.02 7.02 -3.12
Endoplasmic Reticulum -2.98 -13.09 -4.32 -3.11 0.01 1.85 -8.58 2.94 0.87 9.03 -1.41 -15.30 -2.73 -1.58 12.70
Endosome -3.38 5.40 6.35 6.02 1.60 -7.47 -4.54 -0.91 5.36 2.41 -7.17 1.50 1.13 6.48 0.30
Golgi -1.07 18.81 18.29 5.30 -1.38 -3.60 11.85 1.44 3.62 -2.82 -2.72 21.53 3.34 4.53 -2.77
Lipid Particles 0.20 6.39 4.25 2.12 -0.50 -2.09 -4.55 11.95 9.57 10.47 0.52 2.67 15.04 9.65 12.11
Mitochondria -0.85 3.94 4.35 1.89 1.85 -1.34 4.34 2.75 2.93 -3.32 -1.20 5.43 4.42 3.40 -1.82
Mitotic Spindle -1.54 -1.42 -1.59 0.45 0.17 -0.25 1.51 -4.53 -4.44 -5.04 -1.34 -0.76 -4.85 -4.36 -4.35
None 9.42 7.85 1.66 -1.22 -0.72 5.66 11.10 6.06 1.30 -1.13 6.50 12.98 5.69 0.85 -0.74
Nuclear Periphery 0.90 -15.87 -4.83 -5.85 10.00 0.20 -12.86 -7.98 -7.73 8.01 0.70 -20.94 -8.09 -8.51 11.42
Nuclear Periphery Foci -3.81 -12.88 -8.79 -5.76 4.49 0.60 -9.22 -4.96 -5.39 8.57 -2.62 -15.78 -8.77 -6.30 13.45
Nucleolus 8.93 7.72 11.96 6.85 4.44 -0.59 7.63 13.22 3.30 3.81 0.33 10.54 14.66 3.46 3.65
Nucleus 15.03 -12.41 9.34 -0.62 16.59 19.99 1.26 20.10 1.30 6.99 23.72 -9.50 21.92 0.62 19.72
Peroxisomes -3.13 13.25 6.18 5.75 -2.43 -7.53 13.82 3.91 8.87 -2.37 -6.66 17.74 7.17 10.34 -2.81
Vacuole -6.98 -18.28 -17.64 -14.90 -9.96 -7.38 -20.52 -35.74 -32.30 3.32 -9.72 -25.35 -39.29 -34.69 -7.37
Vacuole Periphery -1.70 4.87 7.43 3.55 3.16 -1.37 3.05 -2.82 -2.30 -3.51 -1.96 4.72 -2.77 -2.12 -3.43