Standard name
Description Protein adaptor between Myo4p and the She2p-mRNA complex; part of the mRNA localization machinery that restricts accumulation of certain proteins to the bud; also required for cortical ER inheritance

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.2 0.36 0.36 0.39 0.18 0.14 0.11 0.07 0.1 0.07 0.05 0.07 0.48 0.38 0.22 0.38 0.21 0.39 0.26 0.29 0.35 0.22 0.22 0.25
Bud 0.12 0.17 0.14 0.23 0.23 0.09 0.1 0.1 0.09 0.1 0.1 0.1 0.15 0.17 0.09 0.17 0.09 0.07 0.16 0.19 0.15 0.2 0.18 0.15
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.05 0 0.12 0.13 0.05 0 0 0.05 0.06 0.05 0.06 0.07 0.17 0.13 0.12 0.06 0.09 0.19 0 0 0 0 0 0
Cytoplasm 0.3 0.24 0.17 0.13 0.14 0.15 0.12 0.12 0.12 0.09 0.11 0.09 0.34 0.44 0.52 0.35 0.45 0.36 0.11 0.1 0 0.08 0.1 0.11
Endoplasmic Reticulum 0.11 0.32 0.15 0.15 0.1 0.19 0.09 0.08 0.13 0.07 0.09 0.08 0.18 0.16 0.16 0 0 0.05 0.11 0.14 0.1 0.1 0.13 0.16
Endosome 0.05 0 0 0 0.07 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0
Golgi 0 0.05 0.12 0.07 0 0 0 0 0 0 0 0 0.05 0 0.05 0.23 0.19 0.17 0 0.05 0.06 0.06 0.09 0.05
Mitochondria 0.36 0.1 0.26 0.48 0.46 0.64 0.72 0.66 0.7 0.85 0.79 0.79 0 0 0 0.06 0.09 0.1 0.16 0.05 0.16 0.12 0.13 0.11
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.05 0 0 0 0.08 0 0.06 0.11 0.08 0 0.06 0.07 0 0 0.05 0.11 0.12 0.08 0 0.05 0 0.06 0.05 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 42 44 92 91 51 61 60 41 52 49 26 47 141 115 110 27 14 23 66 47 123 76 92 122
Bud 25 20 36 54 65 41 56 59 46 70 54 68 44 51 44 12 6 4 41 31 51 68 76 74
Bud Neck 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0 7 6 10 13 13 22
Bud Site 3 0 11 6 5 6 4 5 3 6 3 12 6 0 4 1 0 1
Cell Periphery 11 2 30 29 14 12 22 31 32 38 30 45 50 38 63 4 6 11 9 3 11 3 6 11
Cytoplasm 63 29 43 30 41 67 67 70 62 63 58 62 100 133 262 25 30 21 29 16 14 27 42 53
Endoplasmic Reticulum 24 39 39 35 27 81 51 49 69 48 47 54 52 48 81 2 2 3 28 22 35 32 53 76
Endosome 11 0 4 3 19 13 18 32 8 3 1 11 3 1 9 1 0 0 4 6 9 21 12 14
Golgi 7 6 31 17 7 10 15 17 19 8 9 6 15 6 25 16 13 10 11 8 21 20 39 23
Mitochondria 75 12 68 112 129 278 395 397 368 602 416 529 2 2 8 4 6 6 41 8 55 41 53 52
Nucleus 1 0 1 1 0 0 1 1 0 2 3 2 0 1 0 0 0 1 1 0 0 2 1 2
Nuclear Periphery 2 0 0 0 0 1 2 2 5 1 2 5 0 1 0 0 0 0 1 1 1 1 1 2
Nucleolus 3 0 1 3 5 6 5 7 3 5 7 4 1 0 1 0 1 0 0 0 0 1 0 0
Peroxisomes 4 0 11 8 11 4 6 12 1 3 1 3 2 3 10 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 1 0 1 2 1 2 0 1 0 0 2 3 4 0 1 1 0 0 1 2 1 1
Vac/Vac Membrane 11 4 8 8 24 18 35 65 42 31 31 45 8 13 24 8 8 5 10 7 8 21 21 19
Unique Cell Count 210 121 258 231 283 435 550 598 528 706 528 667 291 299 504 71 67 59 256 164 350 339 420 483
Labelled Cell Count 283 156 376 397 399 600 738 791 710 930 688 893 426 415 645 100 88 86 256 164 350 339 420 483


Ambiguous, Bud, Cell Periphery, Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.8 11.6 9.8 9.0 8.8 8.0 7.6 7.5 7.3 7.0 6.8 6.6 11.2 10.7 10.8 12.3 12.5 13.2 10.9 11.1 11.3
Std Deviation (1e-4) 1.9 2.3 1.9 1.7 2.2 1.9 1.7 1.6 1.3 1.4 1.5 1.4 2.4 2.5 2.4 2.7 3.5 3.3 1.8 1.6 1.6
Intensity Change (Log2) -0.11 -0.15 -0.29 -0.37 -0.38 -0.43 -0.48 -0.52 -0.56 0.19 0.12 0.14 0.33 0.36 0.44 0.16 0.18 0.21


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0.9 -4.6 -6.6 -8.4 -10.7 -8.8 -11.2 -11.3 -10.9 3.0 0.7 -4.1 0.4 -2.3 0.5
Bud 2.7 2.7 -1.8 -1.6 -1.7 -2.3 -1.8 -1.5 -1.6 0.4 1.0 -2.2 0.6 -1.1 -1.5
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site -1.0 -1.7 -2.4 0 0 0 0 0 -2.2 -1.5 -3.6 -3.3 0 0 0
Cell Periphery 0.3 -2.8 -4.7 -4.1 -3.4 -2.7 -3.4 -2.9 -2.4 1.8 0.4 0.3 -1.5 -0.6 1.4
Cytoplasm -1.1 -0.7 -0.4 -1.7 -2.0 -1.9 -3.4 -2.2 -3.2 4.7 7.0 9.4 3.4 4.9 3.3
Endoplasmic Reticulum 0 -2.0 1.2 -2.5 -3.1 -0.8 -4.0 -2.6 -3.2 0.9 0.3 0.3 -2.8 -2.7 -2.1
Endosome 0 3.0 1.2 1.4 2.5 0 0 0 0 0 0 0 0 0 0
Golgi -1.7 -4.3 -5.2 -5.3 -5.4 -4.5 -7.6 -6.2 -7.7 -2.9 -4.7 -3.5 2.2 1.6 1.0
Mitochondria 5.1 4.6 9.6 12.2 10.8 11.5 17.6 14.2 15.1 -9.0 -9.1 -10.8 -3.7 -3.0 -2.7
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes -0.5 -0.2 -2.9 -2.9 -1.9 0 0 0 0 -2.8 -2.5 -1.8 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0.2 2.6 0.7 1.9 3.7 2.6 0.9 1.7 2.1 -0.2 0.8 1.1 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 7.1508 8.2755 7.6743 7.6168 6.3615 8.0053 5.1718 6.3799 6.3853 6.0309 5.6826 7.1535 6.0881 6.9375 5.8531 5.9721 4.8948 6.4683
Actin 0.1717 0.1138 0.1044 0.0725 0.2083 0.188 0.2009 0.0803 0.1528 0.0515 0.1327 0.1735 0.2468 0.1146 0.1336 0.1352 0.1983 0.1824
Bud 0.0764 0.0449 0.2712 0.1905 0.1207 0.0206 0.0578 0.0611 0.2827 0.2261 0.1971 0.0284 0.0906 0.0516 0.2881 0.1665 0.1326 0.0186
Bud Neck 0.0083 0.0072 0.0083 0.0071 0.0608 0.2826 0.0083 0.0086 0.0147 0.0395 0.0025 0.2336 0.0129 0.0083 0.0103 0.04 0.0117 0.2612
Bud Periphery 0.1169 0.057 0.3739 0.4725 0.358 0.0421 0.0682 0.0447 0.35 0.4455 0.4295 0.0734 0.1235 0.0384 0.3327 0.3431 0.263 0.0497
Bud Site 0.1197 0.3625 0.1039 0.018 0.0164 0.018 0.128 0.4464 0.0886 0.0154 0.0113 0.0167 0.1295 0.5392 0.0908 0.0377 0.0157 0.0115
Cell Periphery 0.025 0.005 0.0031 0.0075 0.0087 0.01 0.0049 0.0057 0.0034 0.0035 0.0025 0.0067 0.01 0.0044 0.0039 0.0062 0.0023 0.0072
Cytoplasm 0.1829 0.2511 0.0587 0.1135 0.0732 0.1377 0.105 0.2617 0.0375 0.0853 0.0953 0.1523 0.1445 0.1614 0.0523 0.1272 0.0602 0.1097
Cytoplasmic Foci 0.0398 0.0349 0.0119 0.011 0.0102 0.0503 0.0635 0.0315 0.0148 0.0114 0.0197 0.0376 0.0338 0.0242 0.0149 0.0092 0.0514 0.0595
Eisosomes 0.002 0.0003 0.0001 0.0001 0.0007 0.0011 0.001 0.0003 0.0002 0.0001 0.0002 0.0008 0.001 0.0002 0.0003 0.0005 0.0004 0.0008
Endoplasmic Reticulum 0.0327 0.0083 0.0031 0.0027 0.0138 0.0185 0.024 0.0029 0.0009 0.002 0.0029 0.0127 0.0316 0.0016 0.0077 0.0033 0.0043 0.0195
Endosome 0.0579 0.0472 0.0171 0.0099 0.0157 0.05 0.1015 0.0182 0.0123 0.0161 0.0211 0.0814 0.0675 0.0162 0.0157 0.0268 0.0626 0.0854
Golgi 0.0614 0.0347 0.0144 0.0029 0.0161 0.093 0.0859 0.0153 0.0162 0.028 0.0073 0.1052 0.0515 0.0172 0.0151 0.0259 0.0646 0.1283
Lipid Particles 0.008 0.0011 0.002 0.0005 0.0037 0.0036 0.0261 0.0005 0.001 0.0043 0.0056 0.001 0.0051 0.0005 0.0058 0.0049 0.0642 0.0016
Mitochondria 0.0382 0.008 0.0068 0.0607 0.0692 0.0621 0.0468 0.0088 0.0134 0.0471 0.0399 0.0556 0.0168 0.0112 0.0144 0.0273 0.0328 0.0494
None 0.0107 0.0011 0.0102 0.0032 0.0017 0.0019 0.0042 0.0011 0.0005 0.0007 0.001 0.0008 0.0036 0.0004 0.0017 0.0014 0.001 0.0008
Nuclear Periphery 0.0164 0.0021 0.0012 0.0019 0.0061 0.0007 0.0235 0.0005 0.0004 0.0017 0.0017 0.0008 0.0044 0.0005 0.002 0.0029 0.0042 0.0011
Nucleolus 0.0007 0.0003 0.0001 0.0002 0.0003 0.0003 0.003 0.0004 0.0003 0.0003 0.0006 0.0014 0.0008 0.0003 0.0003 0.0009 0.0005 0.0002
Nucleus 0.005 0.0022 0.0008 0.0018 0.0021 0.0009 0.0066 0.0012 0.0008 0.0019 0.0015 0.0026 0.0037 0.0009 0.0013 0.004 0.0014 0.001
Peroxisomes 0.0077 0.0019 0.0023 0.0035 0.0063 0.0079 0.0162 0.0026 0.0035 0.0045 0.0071 0.0018 0.0078 0.0027 0.0022 0.0037 0.0122 0.003
Punctate Nuclear 0.0057 0.0005 0.0002 0.0012 0.0004 0.0008 0.0095 0.0004 0.0004 0.0004 0.0003 0.0003 0.0016 0.0004 0.0015 0.0005 0.0015 0.0005
Vacuole 0.0096 0.0142 0.0048 0.0151 0.0054 0.0074 0.0096 0.0071 0.0046 0.0117 0.0141 0.0091 0.0099 0.0049 0.0041 0.0257 0.01 0.006
Vacuole Periphery 0.0034 0.0017 0.0013 0.0035 0.0022 0.0024 0.0054 0.0007 0.0009 0.0031 0.0061 0.0043 0.0029 0.0009 0.0011 0.007 0.0052 0.0028

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 32.3769 37.8188 25.7723 30.6391 30.7839 45.734 39.7993 30.2854 25.7064 35.069
Translational Efficiency 1.4986 1.4983 1.5249 1.2303 1.3325 0.9409 1.1406 1.4524 1.5491 1.2908

Hit Data

Dataset Hit
Protein Abundance
Protein Localization
Gene Expression
Translational Efficiency