Standard name
Human Ortholog
Description Protein arginine N-methyltransferase; exhibits septin and Hsl1p-dependent localization to the bud neck in budded cells and periodic Hsl1p-dependent phosphorylation; required with Hsl1p, and Elm1p for the mother-bud neck recruitment, phosphorylation, and degradation of Swe1p; interacts directly with Swe1p; relocalizes away from bud neck upon DNA replication stress; human homolog PRMT5 can complement yeast hsl7 mutant
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0 0.07 0 0 0 0 0
Bud 0 0.05 0 0 0.06 0.08 0.05 0.06 0.13 0.08 0.1 0.11 0.05 0 0 0 0 0 0.05 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0.16 0.08 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.05 0 0 0 0 0 0 0
Cytoplasm 0.85 0.91 0.95 0.87 0.82 0.82 0.77 0.79 0.63 0.63 0.69 0.65 0.94 0.93 0.97 0.88 0.83 0.92 0.63 0.72 0.77 0.91 0.88 0.86
Endoplasmic Reticulum 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.11 0.19 0.08 0.32 0.42 0.41 0.35 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0.05 0.08 0 0.1 0.06 0.08 0 0 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 8 16 1 1 7 0 3 2 5 0 0 4 2 4 2 5 6 1 10 24 3 1 3 5
Bud 6 27 2 7 11 11 9 12 21 14 17 18 12 7 5 1 1 0 7 14 2 0 5 5
Bud Neck 2 1 3 4 4 6 4 11 7 2 5 5 0 0 0 0 1 0 25 46 8 2 2 1
Bud Site 0 1 0 0 0 0 0 2 4 7 2 5 0 0 0 0 0 0 – – – – – –
Cell Periphery 3 2 5 2 2 3 3 2 1 0 0 0 1 2 0 9 5 2 0 0 0 0 0 0
Cytoplasm 120 482 182 143 152 120 149 162 102 107 112 107 244 489 505 85 77 47 97 408 146 262 391 379
Endoplasmic Reticulum 7 6 1 0 0 0 0 0 0 0 0 0 1 2 2 8 3 3 0 2 4 2 8 10
Endosome 2 9 0 2 3 1 6 1 0 0 2 0 6 19 8 4 4 0 6 26 2 1 1 2
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 3 0 0 2 1 0 0 1
Mitochondria 0 5 1 4 4 16 36 17 51 71 67 57 1 0 0 1 2 1 0 8 5 7 6 4
Nucleus 0 7 1 2 0 1 3 4 2 5 0 2 0 0 1 0 0 1 0 3 3 0 1 3
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 1 1 1 1 0 1 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 1 2 0 0 0 1 0 1 1 1 1 0 0 0 0 0 0 0
Peroxisomes 1 1 0 0 2 0 1 2 2 1 3 1 1 3 0 0 0 0 1 1 0 0 0 0
SpindlePole 1 2 2 8 14 5 19 13 13 7 3 15 0 2 0 1 0 0 3 8 0 0 2 0
Vac/Vac Membrane 2 7 3 3 1 1 2 7 1 1 3 2 2 7 3 3 3 2 0 15 6 7 13 19
Unique Cell Count 142 531 191 164 186 146 193 206 161 171 163 165 259 525 518 97 93 51 156 569 191 289 443 442
Labelled Cell Count 152 567 201 176 200 164 236 237 209 215 215 218 271 537 527 120 106 57 156 569 191 289 443 442


Bud Neck, Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.0 5.6 6.2 5.6 5.8 4.8 5.1 5.4 4.0 3.9 3.7 4.4 5.6 5.9 5.9 8.8 9.2 7.4 5.4 5.8 6.3
Std Deviation (1e-4) 1.4 0.7 1.9 1.7 1.4 1.6 1.9 2.0 1.0 1.3 0.9 1.4 1.0 0.9 1.0 1.7 2.2 0.9 0.9 1.0 1.1
Intensity Change (Log2) – – – -0.15 -0.11 -0.37 -0.29 -0.21 -0.65 -0.66 -0.74 -0.51 -0.15 -0.09 -0.08 0.5 0.57 0.26 -0.21 -0.09 0.01


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 2.6 0 2.1 2.6 0 0 0 0 2.2 0 0 0 0 0
Bud Neck 0 0 0 0 2.0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -2.7 -4.1 -3.9 -5.1 -4.9 -7.6 -7.7 -6.6 -7.3 -0.5 -1.0 1.5 -2.4 -3.5 -0.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 2.7 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 6.0 3.7 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 3.1 0 3.8 2.7 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.2431 3.6255 3.2719 2.8378 3.2229 3.6099 1.2616 2.0337 1.6782 1.6558 1.5839 1.5414 0.7328 1.5867 0.9014 1.1917 0.9469 1.0385
Actin 0.0585 0.0027 0.038 0.0114 0.037 0.0342 0.0496 0.0124 0.0314 0.0332 0.0205 0.0153 0.0805 0.0054 0.0172 0.033 0.0084 0.0244
Bud 0.0045 0.003 0.0015 0.0017 0.0021 0.0005 0.011 0.0042 0.0072 0.0092 0.0187 0.0019 0.0043 0.0021 0.0014 0.0006 0.0018 0.0012
Bud Neck 0.0057 0.0184 0.5221 0.69 0.6203 0.2025 0.0299 0.0177 0.4534 0.6147 0.5114 0.166 0.0424 0.0414 0.4705 0.695 0.5833 0.194
Bud Periphery 0.0052 0.0014 0.0018 0.0021 0.0041 0.0008 0.0134 0.0046 0.0093 0.0256 0.0186 0.0044 0.0037 0.0013 0.0014 0.0006 0.0019 0.0014
Bud Site 0.0448 0.1562 0.1866 0.0235 0.009 0.0017 0.104 0.227 0.1823 0.014 0.0362 0.0038 0.1496 0.0808 0.163 0.0202 0.0237 0.0077
Cell Periphery 0.003 0.0014 0.0026 0.0027 0.0015 0.0006 0.001 0.0009 0.001 0.0004 0.0017 0.0001 0.0009 0.001 0.0008 0.0005 0.0004 0.0002
Cytoplasm 0.3948 0.5303 0.1173 0.0497 0.2151 0.5139 0.4443 0.5601 0.1615 0.0946 0.1069 0.6644 0.4222 0.6235 0.1353 0.13 0.1523 0.6152
Cytoplasmic Foci 0.0867 0.0381 0.0406 0.0398 0.088 0.0513 0.0901 0.0394 0.0615 0.0427 0.0767 0.0464 0.08 0.0161 0.055 0.0387 0.0324 0.0184
Eisosomes 0.0009 0.0001 0.0005 0.0014 0.0001 0.0002 0.0005 0.0002 0.0002 0.0002 0.0001 0.0001 0.0003 0.0001 0.0003 0.0001 0.0001 0.0001
Endoplasmic Reticulum 0.0118 0.0021 0.0007 0.0012 0.0002 0.0025 0.0079 0.0021 0.0012 0.0013 0.0036 0.0022 0.0048 0.0028 0.0026 0.0005 0.0018 0.0018
Endosome 0.0278 0.002 0.0079 0.0189 0.0013 0.0102 0.0438 0.0142 0.0115 0.045 0.0458 0.0101 0.0174 0.0051 0.0121 0.0111 0.0281 0.0105
Golgi 0.0122 0.0006 0.0086 0.0097 0.0032 0.0038 0.0149 0.003 0.0122 0.0302 0.0167 0.0037 0.0098 0.0014 0.0061 0.0121 0.0113 0.0041
Lipid Particles 0.0148 0.0005 0.0071 0.0388 0.0005 0.0032 0.0178 0.0027 0.0084 0.0221 0.0259 0.0014 0.0094 0.0004 0.0028 0.0231 0.0095 0.0018
Mitochondria 0.0038 0.0004 0.0063 0.0031 0.0009 0.0006 0.0059 0.0009 0.0087 0.031 0.0519 0.0043 0.0042 0.0121 0.0022 0.0049 0.0444 0.0014
None 0.2392 0.2222 0.0303 0.0698 0.0021 0.1423 0.0955 0.086 0.0292 0.0067 0.0296 0.0578 0.1428 0.1989 0.1019 0.0039 0.0625 0.1102
Nuclear Periphery 0.019 0.0008 0.0004 0.0005 0.0001 0.0066 0.0119 0.0014 0.0004 0.0013 0.0015 0.0015 0.0025 0.0007 0.0007 0.0001 0.0016 0.0004
Nucleolus 0.0019 0.0004 0.0005 0.0025 0.0009 0.0004 0.0014 0.0006 0.0005 0.0011 0.0009 0.0002 0.0003 0.0002 0.0009 0.0006 0.0014 0.0001
Nucleus 0.0069 0.0035 0.001 0.001 0.001 0.0034 0.0105 0.0042 0.0019 0.0019 0.0025 0.0037 0.0019 0.0018 0.0012 0.0005 0.0017 0.0011
Peroxisomes 0.0174 0.0029 0.0226 0.0223 0.0039 0.006 0.0205 0.0036 0.0101 0.015 0.0232 0.0065 0.0135 0.0011 0.0196 0.022 0.0255 0.0032
Punctate Nuclear 0.0368 0.0113 0.0027 0.0041 0.0082 0.014 0.0189 0.0104 0.0057 0.0032 0.0042 0.0045 0.0077 0.0009 0.0031 0.0008 0.0055 0.0007
Vacuole 0.0032 0.0014 0.0009 0.0053 0.0005 0.0006 0.0057 0.0042 0.0021 0.005 0.0025 0.0013 0.0013 0.0029 0.0016 0.0012 0.0017 0.0017
Vacuole Periphery 0.0013 0.0001 0.0002 0.0005 0.0001 0.0004 0.0016 0.0003 0.0003 0.0018 0.0011 0.0003 0.0005 0.0001 0.0002 0.0002 0.0007 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 30.8722 34.1499 30.9392 33.6224 31.6762 25.7496 40.451 40.9187 32.8168 39.7223
Translational Efficiency 0.3618 0.3967 0.3587 0.3116 0.3151 0.3289 0.327 0.2872 0.3473 0.3057

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Protein arginine N-methyltransferase; exhibits septin and Hsl1p-dependent localization to the bud neck in budded cells and periodic Hsl1p-dependent phosphorylation; required with Hsl1p, and Elm1p for the mother-bud neck recruitment, phosphorylation, and degradation of Swe1p; interacts directly with Swe1p; relocalizes away from bud neck upon DNA replication stress; human homolog PRMT5 can complement yeast hsl7 mutant
Localization
Cell Percentages cytoplasm (61%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Hsl7

Hsl7


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Hsl7-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available