Standard name | |
---|---|
Human Ortholog | |
Description | Cyclin-dependent protein kinase regulatory subunit and adaptor; interacts with Cdc28p (aka Cdk1p); required for G1/S and G2/M phase transitions and budding; mediates phosphorylation and degradation of Sic1p; modulates proteolysis of M-phase targets through interactions with the proteasome; role in transcriptional regulation, recruiting proteasomal subunits to target gene promoters; human homologs CKS1B and CKS2 can each complement yeast cks1 null mutant |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 1 | 0 | 4 | 6 | 6 | 2 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 |
Bud | 1 | 0 | 4 | 0 | 1 | 2 | – | 3 | 3 | 4 | 4 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 54 | 15 | 11 | 5 | 1 | 12 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 6 | 3 | 0 | 0 | 2 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 2 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 1 | 1 | 1 | 0 | 1 | – | 3 | 0 | 1 | 6 | 6 | 0 | 0 | 2 | 3 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 195 | 88 | 56 | 67 | 62 | 111 | – | 170 | 118 | 140 | 115 | 144 | 89 | 120 | 132 | 131 | 80 | 55 | 127 | 58 | 50 | 99 | 210 | 235 |
Endoplasmic Reticulum | 2 | 0 | 0 | 2 | 1 | 0 | – | 5 | 3 | 1 | 3 | 1 | 0 | 2 | 2 | 9 | 2 | 3 | 0 | 0 | 0 | 0 | 1 | 1 |
Endosome | 1 | 0 | 0 | 0 | 8 | 8 | – | 6 | 1 | 0 | 1 | 2 | 7 | 11 | 22 | 0 | 1 | 2 | 7 | 2 | 3 | 1 | 1 | 6 |
Golgi | 0 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 2 | 7 | 1 | 0 | 0 | 0 | 3 | 0 |
Mitochondria | 5 | 0 | 18 | 33 | 12 | 44 | – | 113 | 119 | 126 | 87 | 121 | 0 | 5 | 4 | 6 | 4 | 3 | 2 | 0 | 1 | 0 | 1 | 0 |
Nucleus | 112 | 44 | 49 | 24 | 70 | 65 | – | 99 | 57 | 51 | 48 | 56 | 78 | 155 | 213 | 22 | 16 | 12 | 195 | 101 | 59 | 34 | 49 | 58 |
Nuclear Periphery | 1 | 0 | 0 | 0 | 0 | 3 | – | 1 | 2 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 1 | 1 |
Nucleolus | 10 | 4 | 4 | 2 | 13 | 14 | – | 35 | 19 | 22 | 13 | 15 | 3 | 14 | 12 | 1 | 5 | 6 | 12 | 8 | 2 | 1 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 14 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 3 | 7 | 20 | 0 | 0 | 0 | 17 | 9 | 7 | 1 | 1 | 4 |
Vac/Vac Membrane | 98 | 73 | 30 | 33 | 57 | 44 | – | 153 | 62 | 75 | 29 | 66 | 87 | 92 | 75 | 96 | 77 | 55 | 20 | 13 | 7 | 14 | 12 | 17 |
Unique Cell Count | 388 | 193 | 128 | 143 | 205 | 253 | 504 | 321 | 369 | 257 | 353 | 241 | 383 | 447 | 241 | 157 | 124 | 455 | 215 | 148 | 163 | 290 | 345 | |
Labelled Cell Count | 426 | 211 | 162 | 162 | 224 | 292 | 588 | 386 | 422 | 308 | 414 | 271 | 414 | 503 | 273 | 188 | 144 | 455 | 215 | 148 | 163 | 290 | 345 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 9.6 | 12.6 | 6.1 | 7.2 | 8.2 | 7.2 | – | 7.2 | 7.1 | 7.4 | 7.5 | 7.7 | 10.4 | 10.7 | 10.4 | 18.4 | 18.5 | 18.0 | 9.7 | 10.6 | 10.5 |
Std Deviation (1e-4) | 1.6 | 1.8 | 1.1 | 1.4 | 1.5 | 1.4 | – | 1.4 | 1.7 | 1.7 | 1.7 | 1.9 | 1.9 | 2.4 | 2.5 | 5.8 | 6.0 | 4.8 | 1.9 | 1.8 | 1.8 |
Intensity Change (Log2) | – | – | – | 0.25 | 0.42 | 0.23 | – | 0.24 | 0.22 | 0.28 | 0.3 | 0.34 | 0.77 | 0.81 | 0.78 | 1.6 | 1.6 | 1.56 | 0.67 | 0.8 | 0.78 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.5 | -2.5 | 0 | – | -2.1 | -1.4 | -1.2 | 0.2 | -0.6 | -1.3 | -2.6 | -3.0 | 1.9 | 1.2 | 0.1 |
Endoplasmic Reticulum | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 1.9 | -2.5 | 0.8 | – | 2.1 | 4.8 | 4.3 | 4.1 | 4.3 | -6.0 | -6.0 | 0 | -4.3 | -3.6 | -3.3 |
Nucleus | -4.0 | -0.8 | -2.5 | – | -4.4 | -4.6 | -5.9 | -4.2 | -5.3 | -1.1 | 0.4 | 1.9 | -6.8 | -5.6 | -5.3 |
Nuclear Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 1.3 | 1.0 | – | 1.6 | 1.2 | 1.2 | 0.9 | 0.6 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -0.1 | 0.9 | -1.4 | – | 1.5 | -1.0 | -0.7 | -3.1 | -1.1 | 2.5 | 0.1 | -1.7 | 3.2 | 4.4 | 3.5 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 5.7496 | 7.5955 | 7.2573 | 6.6186 | 6.3092 | 6.5259 | 4.6288 | 6.6192 | 6.2829 | 5.4695 | 4.8884 | 5.8346 | 6.8671 | 8.7201 | 8.1844 | 8.18 | 8.0455 | 7.896 |
Actin | 0.0294 | 0.0017 | 0.0082 | 0.0249 | 0.0053 | 0.0012 | 0.0105 | 0.0003 | 0.0074 | 0.0128 | 0.0016 | 0.0019 | 0.0101 | 0.0008 | 0.0016 | 0.0077 | 0.0194 | 0.0074 |
Bud | 0.0014 | 0.0005 | 0.0006 | 0.0003 | 0.0011 | 0.0002 | 0.0013 | 0.0007 | 0.0004 | 0.0044 | 0.0005 | 0.0004 | 0.0043 | 0.0009 | 0.0004 | 0.0001 | 0.0006 | 0.0002 |
Bud Neck | 0.0032 | 0.0007 | 0.0015 | 0.0027 | 0.0178 | 0.0034 | 0.0037 | 0.0008 | 0.0021 | 0.0013 | 0.0012 | 0.0068 | 0.0135 | 0.001 | 0.0007 | 0.0006 | 0.0039 | 0.0038 |
Bud Periphery | 0.0013 | 0.0002 | 0.0005 | 0.0005 | 0.002 | 0.0002 | 0.001 | 0.0002 | 0.0003 | 0.0081 | 0.0009 | 0.0002 | 0.0087 | 0.0003 | 0.0003 | 0.0001 | 0.0008 | 0.0001 |
Bud Site | 0.0035 | 0.0023 | 0.0024 | 0.0019 | 0.0104 | 0.0002 | 0.0026 | 0.0023 | 0.0013 | 0.0047 | 0.0034 | 0.0004 | 0.0248 | 0.0062 | 0.0009 | 0.0001 | 0.0024 | 0.0002 |
Cell Periphery | 0.0014 | 0.0001 | 0.0001 | 0.0005 | 0.0006 | 0.0001 | 0.0003 | 0 | 0 | 0.0003 | 0.0004 | 0.0001 | 0.0017 | 0.0001 | 0 | 0 | 0.0001 | 0 |
Cytoplasm | 0.3922 | 0.2488 | 0.0211 | 0.0015 | 0.0079 | 0.2151 | 0.3553 | 0.2009 | 0.0227 | 0.0033 | 0.0203 | 0.2063 | 0.4631 | 0.2774 | 0.0267 | 0.001 | 0.0067 | 0.3333 |
Cytoplasmic Foci | 0.0269 | 0.0031 | 0.0023 | 0.0032 | 0.0178 | 0.0016 | 0.0414 | 0.0008 | 0.0103 | 0.0085 | 0.0255 | 0.0119 | 0.0248 | 0.0012 | 0.0107 | 0.0065 | 0.0031 | 0.0033 |
Eisosomes | 0.0006 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0002 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0.0002 | 0 | 0 | 0.0001 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0156 | 0.0019 | 0.003 | 0.0003 | 0.0028 | 0.0026 | 0.0111 | 0.0018 | 0.001 | 0.0008 | 0.005 | 0.0028 | 0.0082 | 0.0011 | 0.0007 | 0.0002 | 0.0035 | 0.0021 |
Endosome | 0.0197 | 0.0025 | 0.0065 | 0.0106 | 0.021 | 0.0067 | 0.0701 | 0.0008 | 0.0063 | 0.0107 | 0.1077 | 0.0087 | 0.0217 | 0.0004 | 0.0031 | 0.0016 | 0.0045 | 0.0026 |
Golgi | 0.0051 | 0.0003 | 0.0019 | 0.0039 | 0.0063 | 0.0009 | 0.01 | 0 | 0.0037 | 0.0102 | 0.0149 | 0.0073 | 0.0074 | 0.0001 | 0.0016 | 0.0019 | 0.0032 | 0.0015 |
Lipid Particles | 0.0079 | 0.0006 | 0.0026 | 0.0039 | 0.0121 | 0.0015 | 0.0103 | 0 | 0.0024 | 0.015 | 0.032 | 0.001 | 0.0088 | 0 | 0.0049 | 0.0136 | 0.0051 | 0.0024 |
Mitochondria | 0.0072 | 0.0005 | 0.0042 | 0.0045 | 0.0139 | 0.0007 | 0.0245 | 0.0002 | 0.0028 | 0.024 | 0.0137 | 0.0066 | 0.021 | 0.0003 | 0.0013 | 0.0011 | 0.0036 | 0.0004 |
None | 0.0131 | 0.0019 | 0.0012 | 0.0003 | 0.0009 | 0.0005 | 0.0062 | 0.0014 | 0.0006 | 0.0004 | 0.0013 | 0.0021 | 0.0091 | 0.001 | 0.0007 | 0.0007 | 0.0005 | 0.0008 |
Nuclear Periphery | 0.0466 | 0.0159 | 0.0168 | 0.0077 | 0.0277 | 0.0207 | 0.0558 | 0.0127 | 0.0261 | 0.0088 | 0.0167 | 0.0191 | 0.0275 | 0.0106 | 0.0089 | 0.0092 | 0.0453 | 0.0155 |
Nucleolus | 0.0035 | 0.0052 | 0.0081 | 0.0037 | 0.0115 | 0.0016 | 0.002 | 0.0024 | 0.004 | 0.0034 | 0.0074 | 0.0019 | 0.0085 | 0.0047 | 0.0046 | 0.0028 | 0.0241 | 0.0023 |
Nucleus | 0.3813 | 0.7009 | 0.8928 | 0.911 | 0.7927 | 0.7315 | 0.3538 | 0.7567 | 0.886 | 0.8671 | 0.636 | 0.6862 | 0.3128 | 0.6809 | 0.9037 | 0.9349 | 0.8519 | 0.5956 |
Peroxisomes | 0.0056 | 0.0003 | 0.0038 | 0.005 | 0.0065 | 0.0004 | 0.0082 | 0.0001 | 0.0024 | 0.0062 | 0.0038 | 0.0017 | 0.0027 | 0.0001 | 0.0099 | 0.0149 | 0.0065 | 0.0055 |
Punctate Nuclear | 0.0177 | 0.0113 | 0.0208 | 0.0018 | 0.0326 | 0.0057 | 0.0108 | 0.0162 | 0.0187 | 0.0055 | 0.0945 | 0.0323 | 0.0078 | 0.012 | 0.018 | 0.0027 | 0.0126 | 0.0219 |
Vacuole | 0.0119 | 0.001 | 0.0011 | 0.0099 | 0.007 | 0.0047 | 0.0171 | 0.0013 | 0.0008 | 0.0031 | 0.0096 | 0.0018 | 0.011 | 0.0007 | 0.0012 | 0.0002 | 0.0011 | 0.0007 |
Vacuole Periphery | 0.005 | 0.0002 | 0.0005 | 0.0015 | 0.002 | 0.0007 | 0.0038 | 0.0002 | 0.0006 | 0.0013 | 0.0034 | 0.0004 | 0.0023 | 0.0001 | 0.0003 | 0.0001 | 0.0009 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 70.6374 | 80.5649 | 45.2409 | 52.0821 | 60.0036 | 68.1638 | 60.5439 | 56.4654 | 38.3944 | 62.0294 |
Translational Efficiency | 1.5077 | 1.0995 | 1.4645 | 1.3001 | 1.7322 | 1.6571 | 1.3614 | 1.7251 | 1.8729 | 1.4471 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✔ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1280 | 1294 | 436 | 1279 | 1582 | 1539 | 304 | 1424 | 2862 | 2833 | 740 | 2703 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 873.58 | 1068.16 | 1457.85 | 1466.74 | 925.05 | 1060.65 | 1454.20 | 1407.31 | 902.03 | 1064.08 | 1456.35 | 1435.43 |
Standard Deviation | 210.19 | 167.93 | 233.53 | 254.74 | 150.67 | 163.87 | 197.59 | 225.29 | 181.55 | 165.78 | 219.49 | 241.51 |
Intensity Change Log 2 | — | 0.290116 | 0.738831 | 0.747601 | — | 0.197345 | 0.652622 | 0.605337 | — | 0.243149 | 0.695137 | 0.676187 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.001366 | 0.001516 | 0.001733 | 0.003070 | 0.000988 | 0.001990 | 0.002782 | 0.002150 | 0.001157 | 0.001774 | 0.002164 | 0.002585 |
Bud Neck | 0.018381 | 0.018339 | 0.023204 | 0.056265 | 0.021487 | 0.023231 | 0.038817 | 0.036744 | 0.020098 | 0.020997 | 0.029618 | 0.045981 |
Bud Site | 0.014176 | 0.015219 | 0.009285 | 0.029042 | 0.013267 | 0.020345 | 0.019709 | 0.022112 | 0.013673 | 0.018004 | 0.013567 | 0.025391 |
Cell Periphery | 0.000479 | 0.000292 | 0.000414 | 0.000443 | 0.000390 | 0.000388 | 0.000376 | 0.000338 | 0.000430 | 0.000344 | 0.000399 | 0.000387 |
Cytoplasm | 0.232605* | 0.315946* | 0.065602 | 0.050167 | 0.338394* | 0.325784* | 0.039483 | 0.071023 | 0.291081* | 0.321291* | 0.054872 | 0.061155 |
Cytoplasmic Foci | 0.016598 | 0.010670 | 0.001622 | 0.004665 | 0.021394 | 0.023675 | 0.004350 | 0.008206 | 0.019249 | 0.017735 | 0.002743 | 0.006531 |
Eisosomes | 0.000058 | 0.000039 | 0.000035 | 0.000036 | 0.000046 | 0.000070 | 0.000056 | 0.000038 | 0.000052 | 0.000056 | 0.000044 | 0.000037 |
Endoplasmic Reticulum | 0.007731 | 0.006039 | 0.004633 | 0.007010 | 0.006208 | 0.009835 | 0.006332 | 0.005650 | 0.006889 | 0.008101 | 0.005331 | 0.006294 |
Endosome | 0.002741 | 0.003367 | 0.000556 | 0.012028 | 0.002176 | 0.005140 | 0.002506 | 0.015073 | 0.002429 | 0.004330 | 0.001357 | 0.013632 |
Golgi | 0.001554 | 0.000905 | 0.000179 | 0.006599 | 0.000526 | 0.002238 | 0.000296 | 0.010321 | 0.000986 | 0.001629 | 0.000227 | 0.008560 |
Lipid Particles | 0.002138 | 0.001403 | 0.000568 | 0.001018 | 0.001537 | 0.003578 | 0.000843 | 0.001512 | 0.001806 | 0.002584 | 0.000681 | 0.001278 |
Mitochondria | 0.001732 | 0.003670 | 0.002425 | 0.012642 | 0.002160 | 0.003905 | 0.003923 | 0.008514 | 0.001968 | 0.003798 | 0.003040 | 0.010467 |
Mitotic Spindle | 0.006229 | 0.033835 | 0.007839 | 0.110396* | 0.013780 | 0.031091 | 0.033791 | 0.109272* | 0.010403 | 0.032344 | 0.018500 | 0.109804* |
None | 0.016408 | 0.013570 | 0.004196 | 0.010196 | 0.010278 | 0.009212 | 0.007855 | 0.011353 | 0.013019 | 0.011203 | 0.005699 | 0.010805 |
Nuclear Periphery | 0.001400 | 0.001278 | 0.001362 | 0.006601 | 0.001261 | 0.001952 | 0.002229 | 0.002429 | 0.001323 | 0.001644 | 0.001718 | 0.004403 |
Nuclear Periphery Foci | 0.006332 | 0.004034 | 0.000845 | 0.004566 | 0.005461 | 0.007822 | 0.000973 | 0.002882 | 0.005850 | 0.006092 | 0.000898 | 0.003679 |
Nucleolus | 0.017269 | 0.011160 | 0.001495 | 0.004785 | 0.011670 | 0.010003 | 0.002926 | 0.002689 | 0.014174 | 0.010532 | 0.002083 | 0.003681 |
Nucleus | 0.637515* | 0.535325* | 0.860668* | 0.660163* | 0.527010* | 0.485218* | 0.798338* | 0.652624* | 0.576432* | 0.508105* | 0.835062* | 0.656191* |
Peroxisomes | 0.002458 | 0.002153 | 0.000325 | 0.000570 | 0.001480 | 0.003416 | 0.000907 | 0.001034 | 0.001917 | 0.002839 | 0.000564 | 0.000815 |
Vacuole | 0.012357 | 0.020456 | 0.012849 | 0.016693 | 0.020122 | 0.030286 | 0.030544 | 0.025514 | 0.016650 | 0.025796 | 0.020118 | 0.021340 |
Vacuole Periphery | 0.000473 | 0.000784 | 0.000166 | 0.003045 | 0.000366 | 0.000819 | 0.002964 | 0.010522 | 0.000414 | 0.000803 | 0.001315 | 0.006984 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -1.29 | -3.81 | -4.82 | -3.52 | -0.97 | -6.89 | -6.40 | -9.19 | -2.37 | 2.23 | -5.84 | -7.62 | -9.50 | -4.18 | 0.84 |
Bud Neck | -1.69 | -5.15 | -15.31 | -13.90 | -5.68 | -2.93 | -6.51 | -10.35 | -8.13 | 1.53 | -3.30 | -8.08 | -18.14 | -15.64 | -2.79 |
Bud Site | -1.24 | -0.27 | -3.68 | -2.42 | -2.63 | -5.74 | -3.16 | -5.91 | -0.30 | -0.57 | -5.04 | -2.34 | -6.84 | -1.74 | -2.49 |
Cell Periphery | 3.10 | -2.21 | -0.72 | -7.30 | 2.26 | -0.43 | -2.68 | -0.35 | 0.09 | 2.67 | 2.38 | -3.81 | -0.89 | -5.37 | 3.85 |
Cytoplasm | -5.26 | 18.79 | 20.87 | 26.22 | 0.44 | 3.58 | 31.68 | 31.13 | 27.35 | -4.19 | -0.66 | 35.47 | 37.00 | 37.92 | -2.26 |
Cytoplasmic Foci | 4.17 | 7.26 | 9.68 | 9.15 | 1.71 | -0.92 | 11.95 | 12.82 | 11.90 | -0.68 | 1.62 | 13.15 | 16.04 | 14.45 | 1.02 |
Eisosomes | 2.55 | 2.18 | 1.65 | -1.57 | -1.04 | -7.62 | -3.58 | -2.80 | 5.53 | 2.58 | -3.12 | -1.45 | -0.25 | 3.62 | 1.43 |
Endoplasmic Reticulum | 1.14 | 3.56 | 1.27 | 0.20 | -2.82 | -6.48 | -1.81 | -0.25 | 6.83 | 1.75 | -3.87 | 1.59 | 0.83 | 5.30 | -0.99 |
Endosome | -0.47 | 6.38 | 0.37 | 0.69 | -3.12 | -7.02 | 3.85 | -0.70 | 3.44 | -2.47 | -5.44 | 8.06 | -0.27 | 3.12 | -4.07 |
Golgi | 1.03 | 2.21 | 0.25 | -0.78 | -2.04 | -2.94 | 1.01 | -1.89 | 0.20 | -2.14 | -1.40 | 2.43 | -1.29 | -0.19 | -2.91 |
Lipid Particles | 2.32 | 3.50 | 1.00 | -2.10 | -4.01 | -4.94 | -0.09 | -0.95 | 4.59 | -0.63 | -3.04 | 2.82 | 0.16 | 3.45 | -3.33 |
Mitochondria | -1.77 | -5.67 | -8.84 | -4.72 | -6.74 | -1.58 | -5.52 | -5.29 | -2.89 | -0.15 | -2.34 | -6.57 | -9.87 | -5.51 | -5.95 |
Mitotic Spindle | -6.70 | -2.31 | -13.23 | -7.46 | -8.96 | -6.06 | -2.76 | -11.71 | -6.95 | -5.07 | -8.89 | -3.29 | -17.46 | -10.16 | -10.18 |
None | 2.03 | 9.82 | 5.37 | 3.02 | -7.45 | 1.67 | 2.49 | 0.55 | -1.16 | -2.14 | 2.57 | 9.31 | 4.34 | 1.64 | -6.41 |
Nuclear Periphery | -0.68 | -3.86 | -10.80 | -10.77 | -9.46 | -4.44 | -7.85 | -17.03 | -10.95 | -3.79 | -3.84 | -8.23 | -15.23 | -13.75 | -10.59 |
Nuclear Periphery Foci | 2.04 | 3.99 | 5.11 | 3.78 | 0.04 | -1.62 | 5.72 | 5.76 | 7.22 | -0.76 | -0.08 | 6.21 | 7.67 | 8.09 | -0.10 |
Nucleolus | 1.82 | 8.32 | 7.04 | 4.89 | -2.46 | -1.35 | 4.84 | 5.13 | 6.20 | -0.71 | 0.31 | 9.49 | 8.60 | 7.86 | -2.31 |
Nucleus | 6.43 | -12.10 | -1.06 | -7.50 | 11.19 | 4.35 | -11.74 | -10.01 | -14.19 | 5.00 | 7.40 | -18.10 | -8.20 | -15.52 | 11.66 |
Peroxisomes | 0.53 | 4.82 | 5.99 | 5.90 | 2.30 | -4.89 | 3.46 | 3.97 | 7.11 | 0.49 | -3.20 | 6.06 | 7.26 | 9.21 | 1.48 |
Vacuole | -4.21 | -2.90 | -10.24 | -7.31 | -6.10 | -5.93 | -3.62 | -9.88 | -5.74 | -3.16 | -7.21 | -4.31 | -14.09 | -9.00 | -6.81 |
Vacuole Periphery | -0.97 | 2.04 | -1.16 | -0.10 | -2.05 | -2.06 | -0.80 | -2.56 | -1.84 | -1.59 | -2.07 | -0.22 | -2.81 | -1.68 | -2.39 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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