Standard name
Human Ortholog
Description Cyclin-dependent protein kinase regulatory subunit and adaptor; interacts with Cdc28p (aka Cdk1p); required for G1/S and G2/M phase transitions and budding; mediates phosphorylation and degradation of Sic1p; modulates proteolysis of M-phase targets through interactions with the proteasome; role in transcriptional regulation, recruiting proteasomal subunits to target gene promoters; human homologs CKS1B and CKS2 can each complement yeast cks1 null mutant

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.12 0.07 0.08 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.5 0.46 0.44 0.47 0.3 0.44 0.34 0.37 0.38 0.45 0.41 0.37 0.31 0.3 0.54 0.51 0.44 0.28 0.27 0.34 0.61 0.73 0.68
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Mitochondria 0 0 0.14 0.23 0.06 0.17 0.22 0.37 0.34 0.34 0.34 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.29 0.23 0.38 0.17 0.34 0.26 0.2 0.18 0.14 0.19 0.16 0.32 0.4 0.48 0.09 0.1 0.1 0.43 0.47 0.4 0.21 0.17 0.17
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0.06 0.06 0.07 0.06 0.06 0.05 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0
Vac/Vac Membrane 0.25 0.38 0.23 0.23 0.28 0.17 0.3 0.19 0.2 0.11 0.19 0.36 0.24 0.17 0.4 0.49 0.44 0 0.06 0.05 0.09 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 1 1 0 4 6 6 2 0 0 2 0 0 0 0 0
Bud 1 0 4 0 1 2 3 3 4 4 1 0 0 0 0 0 0 54 15 11 5 1 12
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 6 3 0 0 2 2
Bud Site 0 0 0 0 0 0 0 2 0 1 1 0 0 0 0 0 0
Cell Periphery 1 1 1 1 0 1 3 0 1 6 6 0 0 2 3 1 1 0 0 0 0 0 0
Cytoplasm 195 88 56 67 62 111 170 118 140 115 144 89 120 132 131 80 55 127 58 50 99 210 235
Endoplasmic Reticulum 2 0 0 2 1 0 5 3 1 3 1 0 2 2 9 2 3 0 0 0 0 1 1
Endosome 1 0 0 0 8 8 6 1 0 1 2 7 11 22 0 1 2 7 2 3 1 1 6
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 0 0 3 2 7 1 0 0 0 3 0
Mitochondria 5 0 18 33 12 44 113 119 126 87 121 0 5 4 6 4 3 2 0 1 0 1 0
Nucleus 112 44 49 24 70 65 99 57 51 48 56 78 155 213 22 16 12 195 101 59 34 49 58
Nuclear Periphery 1 0 0 0 0 3 1 2 1 0 1 0 0 1 0 0 0 2 0 0 0 1 1
Nucleolus 10 4 4 2 13 14 35 19 22 13 15 3 14 12 1 5 6 12 8 2 1 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 2 14 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 3 7 20 0 0 0 17 9 7 1 1 4
Vac/Vac Membrane 98 73 30 33 57 44 153 62 75 29 66 87 92 75 96 77 55 20 13 7 14 12 17
Unique Cell Count 388 193 128 143 205 253 504 321 369 257 353 241 383 447 241 157 124 455 215 148 163 290 345
Labelled Cell Count 426 211 162 162 224 292 588 386 422 308 414 271 414 503 273 188 144 455 215 148 163 290 345


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.6 12.6 6.1 7.2 8.2 7.2 7.2 7.1 7.4 7.5 7.7 10.4 10.7 10.4 18.4 18.5 18.0 9.7 10.6 10.5
Std Deviation (1e-4) 1.6 1.8 1.1 1.4 1.5 1.4 1.4 1.7 1.7 1.7 1.9 1.9 2.4 2.5 5.8 6.0 4.8 1.9 1.8 1.8
Intensity Change (Log2) 0.25 0.42 0.23 0.24 0.22 0.28 0.3 0.34 0.77 0.81 0.78 1.6 1.6 1.56 0.67 0.8 0.78


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.5 -2.5 0 -2.1 -1.4 -1.2 0.2 -0.6 -1.3 -2.6 -3.0 1.9 1.2 0.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 1.9 -2.5 0.8 2.1 4.8 4.3 4.1 4.3 -6.0 -6.0 0 -4.3 -3.6 -3.3
Nucleus -4.0 -0.8 -2.5 -4.4 -4.6 -5.9 -4.2 -5.3 -1.1 0.4 1.9 -6.8 -5.6 -5.3
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 1.3 1.0 1.6 1.2 1.2 0.9 0.6 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -0.1 0.9 -1.4 1.5 -1.0 -0.7 -3.1 -1.1 2.5 0.1 -1.7 3.2 4.4 3.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.7496 7.5955 7.2573 6.6186 6.3092 6.5259 4.6288 6.6192 6.2829 5.4695 4.8884 5.8346 6.8671 8.7201 8.1844 8.18 8.0455 7.896
Actin 0.0294 0.0017 0.0082 0.0249 0.0053 0.0012 0.0105 0.0003 0.0074 0.0128 0.0016 0.0019 0.0101 0.0008 0.0016 0.0077 0.0194 0.0074
Bud 0.0014 0.0005 0.0006 0.0003 0.0011 0.0002 0.0013 0.0007 0.0004 0.0044 0.0005 0.0004 0.0043 0.0009 0.0004 0.0001 0.0006 0.0002
Bud Neck 0.0032 0.0007 0.0015 0.0027 0.0178 0.0034 0.0037 0.0008 0.0021 0.0013 0.0012 0.0068 0.0135 0.001 0.0007 0.0006 0.0039 0.0038
Bud Periphery 0.0013 0.0002 0.0005 0.0005 0.002 0.0002 0.001 0.0002 0.0003 0.0081 0.0009 0.0002 0.0087 0.0003 0.0003 0.0001 0.0008 0.0001
Bud Site 0.0035 0.0023 0.0024 0.0019 0.0104 0.0002 0.0026 0.0023 0.0013 0.0047 0.0034 0.0004 0.0248 0.0062 0.0009 0.0001 0.0024 0.0002
Cell Periphery 0.0014 0.0001 0.0001 0.0005 0.0006 0.0001 0.0003 0 0 0.0003 0.0004 0.0001 0.0017 0.0001 0 0 0.0001 0
Cytoplasm 0.3922 0.2488 0.0211 0.0015 0.0079 0.2151 0.3553 0.2009 0.0227 0.0033 0.0203 0.2063 0.4631 0.2774 0.0267 0.001 0.0067 0.3333
Cytoplasmic Foci 0.0269 0.0031 0.0023 0.0032 0.0178 0.0016 0.0414 0.0008 0.0103 0.0085 0.0255 0.0119 0.0248 0.0012 0.0107 0.0065 0.0031 0.0033
Eisosomes 0.0006 0 0.0001 0.0001 0.0001 0 0.0002 0 0 0.0001 0.0001 0 0.0002 0 0 0.0001 0.0001 0
Endoplasmic Reticulum 0.0156 0.0019 0.003 0.0003 0.0028 0.0026 0.0111 0.0018 0.001 0.0008 0.005 0.0028 0.0082 0.0011 0.0007 0.0002 0.0035 0.0021
Endosome 0.0197 0.0025 0.0065 0.0106 0.021 0.0067 0.0701 0.0008 0.0063 0.0107 0.1077 0.0087 0.0217 0.0004 0.0031 0.0016 0.0045 0.0026
Golgi 0.0051 0.0003 0.0019 0.0039 0.0063 0.0009 0.01 0 0.0037 0.0102 0.0149 0.0073 0.0074 0.0001 0.0016 0.0019 0.0032 0.0015
Lipid Particles 0.0079 0.0006 0.0026 0.0039 0.0121 0.0015 0.0103 0 0.0024 0.015 0.032 0.001 0.0088 0 0.0049 0.0136 0.0051 0.0024
Mitochondria 0.0072 0.0005 0.0042 0.0045 0.0139 0.0007 0.0245 0.0002 0.0028 0.024 0.0137 0.0066 0.021 0.0003 0.0013 0.0011 0.0036 0.0004
None 0.0131 0.0019 0.0012 0.0003 0.0009 0.0005 0.0062 0.0014 0.0006 0.0004 0.0013 0.0021 0.0091 0.001 0.0007 0.0007 0.0005 0.0008
Nuclear Periphery 0.0466 0.0159 0.0168 0.0077 0.0277 0.0207 0.0558 0.0127 0.0261 0.0088 0.0167 0.0191 0.0275 0.0106 0.0089 0.0092 0.0453 0.0155
Nucleolus 0.0035 0.0052 0.0081 0.0037 0.0115 0.0016 0.002 0.0024 0.004 0.0034 0.0074 0.0019 0.0085 0.0047 0.0046 0.0028 0.0241 0.0023
Nucleus 0.3813 0.7009 0.8928 0.911 0.7927 0.7315 0.3538 0.7567 0.886 0.8671 0.636 0.6862 0.3128 0.6809 0.9037 0.9349 0.8519 0.5956
Peroxisomes 0.0056 0.0003 0.0038 0.005 0.0065 0.0004 0.0082 0.0001 0.0024 0.0062 0.0038 0.0017 0.0027 0.0001 0.0099 0.0149 0.0065 0.0055
Punctate Nuclear 0.0177 0.0113 0.0208 0.0018 0.0326 0.0057 0.0108 0.0162 0.0187 0.0055 0.0945 0.0323 0.0078 0.012 0.018 0.0027 0.0126 0.0219
Vacuole 0.0119 0.001 0.0011 0.0099 0.007 0.0047 0.0171 0.0013 0.0008 0.0031 0.0096 0.0018 0.011 0.0007 0.0012 0.0002 0.0011 0.0007
Vacuole Periphery 0.005 0.0002 0.0005 0.0015 0.002 0.0007 0.0038 0.0002 0.0006 0.0013 0.0034 0.0004 0.0023 0.0001 0.0003 0.0001 0.0009 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 70.6374 80.5649 45.2409 52.0821 60.0036 68.1638 60.5439 56.4654 38.3944 62.0294
Translational Efficiency 1.5077 1.0995 1.4645 1.3001 1.7322 1.6571 1.3614 1.7251 1.8729 1.4471

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1280 1294 436 1279 1582 1539 304 1424 2862 2833 740 2703

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 873.58 1068.16 1457.85 1466.74 925.05 1060.65 1454.20 1407.31 902.03 1064.08 1456.35 1435.43
Standard Deviation 210.19 167.93 233.53 254.74 150.67 163.87 197.59 225.29 181.55 165.78 219.49 241.51
Intensity Change Log 2 0.290116 0.738831 0.747601 0.197345 0.652622 0.605337 0.243149 0.695137 0.676187

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001366 0.001516 0.001733 0.003070 0.000988 0.001990 0.002782 0.002150 0.001157 0.001774 0.002164 0.002585
Bud Neck 0.018381 0.018339 0.023204 0.056265 0.021487 0.023231 0.038817 0.036744 0.020098 0.020997 0.029618 0.045981
Bud Site 0.014176 0.015219 0.009285 0.029042 0.013267 0.020345 0.019709 0.022112 0.013673 0.018004 0.013567 0.025391
Cell Periphery 0.000479 0.000292 0.000414 0.000443 0.000390 0.000388 0.000376 0.000338 0.000430 0.000344 0.000399 0.000387
Cytoplasm 0.232605* 0.315946* 0.065602 0.050167 0.338394* 0.325784* 0.039483 0.071023 0.291081* 0.321291* 0.054872 0.061155
Cytoplasmic Foci 0.016598 0.010670 0.001622 0.004665 0.021394 0.023675 0.004350 0.008206 0.019249 0.017735 0.002743 0.006531
Eisosomes 0.000058 0.000039 0.000035 0.000036 0.000046 0.000070 0.000056 0.000038 0.000052 0.000056 0.000044 0.000037
Endoplasmic Reticulum 0.007731 0.006039 0.004633 0.007010 0.006208 0.009835 0.006332 0.005650 0.006889 0.008101 0.005331 0.006294
Endosome 0.002741 0.003367 0.000556 0.012028 0.002176 0.005140 0.002506 0.015073 0.002429 0.004330 0.001357 0.013632
Golgi 0.001554 0.000905 0.000179 0.006599 0.000526 0.002238 0.000296 0.010321 0.000986 0.001629 0.000227 0.008560
Lipid Particles 0.002138 0.001403 0.000568 0.001018 0.001537 0.003578 0.000843 0.001512 0.001806 0.002584 0.000681 0.001278
Mitochondria 0.001732 0.003670 0.002425 0.012642 0.002160 0.003905 0.003923 0.008514 0.001968 0.003798 0.003040 0.010467
Mitotic Spindle 0.006229 0.033835 0.007839 0.110396* 0.013780 0.031091 0.033791 0.109272* 0.010403 0.032344 0.018500 0.109804*
None 0.016408 0.013570 0.004196 0.010196 0.010278 0.009212 0.007855 0.011353 0.013019 0.011203 0.005699 0.010805
Nuclear Periphery 0.001400 0.001278 0.001362 0.006601 0.001261 0.001952 0.002229 0.002429 0.001323 0.001644 0.001718 0.004403
Nuclear Periphery Foci 0.006332 0.004034 0.000845 0.004566 0.005461 0.007822 0.000973 0.002882 0.005850 0.006092 0.000898 0.003679
Nucleolus 0.017269 0.011160 0.001495 0.004785 0.011670 0.010003 0.002926 0.002689 0.014174 0.010532 0.002083 0.003681
Nucleus 0.637515* 0.535325* 0.860668* 0.660163* 0.527010* 0.485218* 0.798338* 0.652624* 0.576432* 0.508105* 0.835062* 0.656191*
Peroxisomes 0.002458 0.002153 0.000325 0.000570 0.001480 0.003416 0.000907 0.001034 0.001917 0.002839 0.000564 0.000815
Vacuole 0.012357 0.020456 0.012849 0.016693 0.020122 0.030286 0.030544 0.025514 0.016650 0.025796 0.020118 0.021340
Vacuole Periphery 0.000473 0.000784 0.000166 0.003045 0.000366 0.000819 0.002964 0.010522 0.000414 0.000803 0.001315 0.006984

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.29 -3.81 -4.82 -3.52 -0.97 -6.89 -6.40 -9.19 -2.37 2.23 -5.84 -7.62 -9.50 -4.18 0.84
Bud Neck -1.69 -5.15 -15.31 -13.90 -5.68 -2.93 -6.51 -10.35 -8.13 1.53 -3.30 -8.08 -18.14 -15.64 -2.79
Bud Site -1.24 -0.27 -3.68 -2.42 -2.63 -5.74 -3.16 -5.91 -0.30 -0.57 -5.04 -2.34 -6.84 -1.74 -2.49
Cell Periphery 3.10 -2.21 -0.72 -7.30 2.26 -0.43 -2.68 -0.35 0.09 2.67 2.38 -3.81 -0.89 -5.37 3.85
Cytoplasm -5.26 18.79 20.87 26.22 0.44 3.58 31.68 31.13 27.35 -4.19 -0.66 35.47 37.00 37.92 -2.26
Cytoplasmic Foci 4.17 7.26 9.68 9.15 1.71 -0.92 11.95 12.82 11.90 -0.68 1.62 13.15 16.04 14.45 1.02
Eisosomes 2.55 2.18 1.65 -1.57 -1.04 -7.62 -3.58 -2.80 5.53 2.58 -3.12 -1.45 -0.25 3.62 1.43
Endoplasmic Reticulum 1.14 3.56 1.27 0.20 -2.82 -6.48 -1.81 -0.25 6.83 1.75 -3.87 1.59 0.83 5.30 -0.99
Endosome -0.47 6.38 0.37 0.69 -3.12 -7.02 3.85 -0.70 3.44 -2.47 -5.44 8.06 -0.27 3.12 -4.07
Golgi 1.03 2.21 0.25 -0.78 -2.04 -2.94 1.01 -1.89 0.20 -2.14 -1.40 2.43 -1.29 -0.19 -2.91
Lipid Particles 2.32 3.50 1.00 -2.10 -4.01 -4.94 -0.09 -0.95 4.59 -0.63 -3.04 2.82 0.16 3.45 -3.33
Mitochondria -1.77 -5.67 -8.84 -4.72 -6.74 -1.58 -5.52 -5.29 -2.89 -0.15 -2.34 -6.57 -9.87 -5.51 -5.95
Mitotic Spindle -6.70 -2.31 -13.23 -7.46 -8.96 -6.06 -2.76 -11.71 -6.95 -5.07 -8.89 -3.29 -17.46 -10.16 -10.18
None 2.03 9.82 5.37 3.02 -7.45 1.67 2.49 0.55 -1.16 -2.14 2.57 9.31 4.34 1.64 -6.41
Nuclear Periphery -0.68 -3.86 -10.80 -10.77 -9.46 -4.44 -7.85 -17.03 -10.95 -3.79 -3.84 -8.23 -15.23 -13.75 -10.59
Nuclear Periphery Foci 2.04 3.99 5.11 3.78 0.04 -1.62 5.72 5.76 7.22 -0.76 -0.08 6.21 7.67 8.09 -0.10
Nucleolus 1.82 8.32 7.04 4.89 -2.46 -1.35 4.84 5.13 6.20 -0.71 0.31 9.49 8.60 7.86 -2.31
Nucleus 6.43 -12.10 -1.06 -7.50 11.19 4.35 -11.74 -10.01 -14.19 5.00 7.40 -18.10 -8.20 -15.52 11.66
Peroxisomes 0.53 4.82 5.99 5.90 2.30 -4.89 3.46 3.97 7.11 0.49 -3.20 6.06 7.26 9.21 1.48
Vacuole -4.21 -2.90 -10.24 -7.31 -6.10 -5.93 -3.62 -9.88 -5.74 -3.16 -7.21 -4.31 -14.09 -9.00 -6.81
Vacuole Periphery -0.97 2.04 -1.16 -0.10 -2.05 -2.06 -0.80 -2.56 -1.84 -1.59 -2.07 -0.22 -2.81 -1.68 -2.39