Standard name
Human Ortholog
Description Nucleolar S-adenosylmethionine-dependent rRNA methyltransferase; methylates adenine (m1A) of the large subunit (LSU) rRNA at position 2142; belongs to Rossmann fold superfamily; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YBR141C is not an essential gene
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0.06 0.06 0.05 0.06 0.05 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0.09 0 0.05 0 0.07 0 0.05 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0.06 0.05 0.06 0.07 0.08 0.05 0.06 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.05 0.06 0.07 0.2 0.18 0.22 0.29 0.19 0.22 0.18 0.19 0.1 0.12 0.21 0.09 0 0.07 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0.07 0.06 0 0 0 0 0 0 0 0.08 0.21 0.06 0.05 0.05 0 0.06 0 0.11 0.16 0.18
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.49 0.09 0.09 0.06 0.1 0.39 0.54 0.32 0.7 0.55 0.78 0.54 0.06 0 0 0.09 0.07 0.16 0 0 0 0 0.05 0
Nucleus 0.61 0.68 0.77 0.16 0.31 0.15 0.11 0.08 0.1 0.13 0.11 0.09 0.39 0.33 0.27 0.41 0.44 0.3 0.32 0.39 0.34 0.15 0.09 0.05
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0
Nucleolus 0.21 0.22 0.07 0.5 0.11 0.12 0.06 0.06 0.06 0.07 0.05 0 0.42 0.4 0.21 0.39 0.5 0.54 0.4 0.36 0.34 0.38 0.29 0.27
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.16 0.69 0.37 0.37 0.32 0.35 0.23 0.27 0.16 0.29 0.44 0.51 0.47 0.2 0.07 0.1 0.06 0 0.09 0.22 0.22 0.22
Vac/Vac Membrane 0.05 0.05 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0.07 0.07 0.09 0.08 0.06 0.07 0.09
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1 2
Bud 0 0 1 3 3 7 15 13 14 17 15 18 2 1 1 3 0 0 3 1 1 2 7 11
Bud Neck 0 0 0 16 9 12 8 15 8 15 7 15 6 22 9 0 0 0 0 1 2 3 12 12
Bud Site 1 0 1 2 7 13 14 13 19 23 14 14 2 2 0 0 0 2 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 1 0 0 0 0 0 0
Cytoplasm 7 8 8 13 40 41 56 65 53 63 52 48 29 90 116 17 5 12 1 2 1 1 10 18
Endoplasmic Reticulum 1 0 0 0 0 0 0 1 0 0 0 0 0 0 1 7 3 3 0 0 0 0 2 2
Endosome 0 2 1 6 14 14 4 4 3 1 1 1 9 60 117 12 6 9 16 11 4 35 87 103
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 2 2 5 1 1 0 1 1 4
Mitochondria 183 15 13 11 21 89 140 73 198 155 227 136 17 10 12 18 8 28 7 0 4 7 27 21
Nucleus 228 109 107 28 63 34 28 19 28 38 33 23 117 245 151 79 52 51 125 78 55 48 49 31
Nuclear Periphery 11 3 1 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 23 2 5 0 8 8
Nucleolus 78 35 10 88 22 28 16 13 16 19 15 8 125 298 121 76 59 93 157 72 54 123 159 155
Peroxisomes 0 0 0 0 1 1 2 0 1 0 0 1 0 2 7 0 1 0 0 0 0 0 2 1
SpindlePole 7 2 22 122 76 85 83 80 65 75 48 74 133 382 267 39 8 17 23 6 15 73 120 129
Vac/Vac Membrane 19 8 3 1 10 6 3 3 4 4 8 6 2 4 13 11 6 12 29 18 13 20 40 52
Unique Cell Count 373 160 139 177 203 231 259 227 281 283 292 254 300 742 564 195 118 172 394 198 161 326 547 581
Labelled Cell Count 535 182 167 290 267 330 369 300 409 410 420 344 442 1120 816 267 150 233 394 198 161 326 547 581


Nucleolus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.3 5.9 5.2 5.3 5.5 4.3 3.8 4.4 3.2 3.5 3.1 3.5 5.5 5.6 5.5 8.2 7.7 7.3 5.9 5.6 6.1
Std Deviation (1e-4) 0.6 1.2 0.8 1.7 2.6 2.3 2.0 2.0 0.9 1.0 0.9 0.7 1.1 1.0 1.9 1.7 1.7 1.9 1.5 1.9 2.0
Intensity Change (Log2) – – – 0.02 0.07 -0.28 -0.44 -0.24 -0.7 -0.55 -0.75 -0.58 0.08 0.11 0.08 0.66 0.57 0.5 0.18 0.12 0.23

WT3RAP60RAP140RAP220RAP300RAP380RAP460RAP540RAP620RAP70002468WT3HU80HU120HU16002468WT3rpd3Δ_1rpd3Δ_2rpd3Δ_302468WT1AF100AF140AF18002468
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 3.6 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.6 3.6 3.3 4.1 5.3 3.6 4.3 3.4 3.6 1.4 2.2 4.1 1.0 -0.6 0.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 2.7 0 0 0 0 0 0 0 0 3.1 5.7 2.5 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -1.0 0.3 0 0 0 0 0 0 0 -1.4 0 0 0 -0.8 1.8
Nucleus -10.9 -8.3 -11.9 -13.3 -13.4 -13.8 -12.9 -13.6 -13.7 -7.4 -9.7 -11.0 -6.6 -5.4 -8.3
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 8.1 1.1 0 0 0 0 0 0 0 7.3 7.5 3.9 6.5 7.7 8.7
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 9.4 4.3 0 0 0 0 0 0 0 5.8 7.7 6.8 1.0 -2.3 -1.6
Vacuole 0 1.3 0 0 0 0 0 0 0 0 0 0 1.6 0 2.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.4931 0.6998 0.5234 0.1875 0.0437 0.4062 0.4229 1.0461 0.9916 0.7874 0.767 0.992 1.0347 1.1208 0.6901 1.2231 1.352 0.7453
Actin 0.0142 0 0.0004 0 0 0.0125 0.0048 0.0051 0.0133 0.0013 0.0084 0.0036 0.0215 0 0.0087 0.0577 0.0058 0.0002
Bud 0.0005 0.0001 0.0002 0.0001 0.0001 0.0002 0.0004 0.0005 0.0001 0.0002 0.0001 0.0001 0.0004 0.0001 0.0002 0.0003 0.0002 0.0001
Bud Neck 0.0071 0.0017 0.002 0.0006 0.0034 0.0047 0.003 0.002 0.002 0.0059 0.002 0.0114 0.0021 0.0054 0.0029 0.0017 0.0015 0.0039
Bud Periphery 0.001 0.0002 0.0005 0.0002 0.0002 0.0004 0.0013 0.0003 0.0003 0.0007 0.0006 0.0004 0.001 0.0002 0.0004 0.0006 0.0005 0.0003
Bud Site 0.0084 0.0016 0.0026 0.0005 0.0012 0.0007 0.0034 0.0015 0.003 0.0008 0.0003 0.0007 0.0025 0.0014 0.0025 0.0013 0.0031 0.0006
Cell Periphery 0.0004 0.0004 0.0004 0.0001 0.0001 0.0002 0.001 0.0007 0.0003 0.0009 0.0001 0.0002 0.0004 0.0006 0.0003 0.0005 0.0002 0.0002
Cytoplasm 0.006 0.0005 0.0009 0.0001 0.0001 0.0122 0.0159 0.0022 0.0007 0.0015 0.0026 0.0003 0.0013 0.0022 0.0003 0.0002 0.0006 0.0001
Cytoplasmic Foci 0.0177 0.0065 0.0059 0.0002 0.001 0.0073 0.0066 0.0033 0.0031 0.0036 0.002 0.0072 0.0187 0.0078 0.0116 0.0069 0.0116 0.0024
Eisosomes 0.0005 0 0 0 0 0.0001 0.0002 0 0.0003 0.0001 0.0003 0.0001 0.0003 0 0.0001 0.0001 0.0001 0
Endoplasmic Reticulum 0.0029 0.0002 0.0005 0 0 0.0004 0.0021 0.0006 0.0005 0.0032 0.0044 0.0003 0.0015 0.0001 0.0004 0.0002 0.0009 0.0001
Endosome 0.0173 0.0041 0.0066 0.0001 0.0003 0.0055 0.0096 0.0046 0.002 0.0337 0.0181 0.0062 0.024 0.0019 0.0053 0.0083 0.0285 0.0016
Golgi 0.0044 0.0006 0.0017 0 0.0002 0.0025 0.0041 0.0008 0.0013 0.0117 0.0054 0.0033 0.011 0.0008 0.0029 0.0245 0.0148 0.0008
Lipid Particles 0.0212 0.018 0.0238 0.0011 0.0015 0.0278 0.0249 0.0193 0.0153 0.0606 0.0451 0.05 0.0627 0.0303 0.0227 0.0435 0.0677 0.0131
Mitochondria 0.0083 0.0002 0.0015 0.0003 0.0002 0.0029 0.0192 0.0006 0.0016 0.0576 0.0392 0.0011 0.0104 0.0003 0.0085 0.0089 0.0049 0.0021
None 0.0035 0.0003 0.0021 0.0001 0.0002 0.0032 0.0006 0.0018 0.0013 0.0054 0.0073 0.0005 0.0031 0.0128 0.0003 0.0003 0.0013 0.0002
Nuclear Periphery 0.0267 0.0062 0.0079 0.0003 0.0027 0.0078 0.0033 0.0066 0.0075 0.0241 0.0667 0.0131 0.015 0.0037 0.0028 0.0041 0.0515 0.0062
Nucleolus 0.5281 0.6829 0.7739 0.6054 0.6274 0.5824 0.721 0.7726 0.8183 0.5731 0.4115 0.6555 0.6373 0.7068 0.7759 0.647 0.542 0.7409
Nucleus 0.1673 0.2086 0.1028 0.3879 0.2395 0.1847 0.1103 0.1318 0.1028 0.1838 0.2401 0.1438 0.0952 0.1666 0.1183 0.1521 0.1646 0.1415
Peroxisomes 0.0092 0.0013 0.0018 0.0001 0.0002 0.0027 0.0067 0.0007 0.0028 0.0015 0.0032 0.0041 0.0121 0.0008 0.011 0.0026 0.0138 0.0011
Punctate Nuclear 0.1487 0.06 0.06 0.0023 0.1211 0.1376 0.0522 0.0316 0.0217 0.0132 0.1368 0.0964 0.0747 0.053 0.0231 0.0356 0.0829 0.0827
Vacuole 0.004 0.0061 0.0037 0.0003 0.0004 0.0024 0.0052 0.0112 0.0013 0.0118 0.0013 0.0012 0.003 0.0044 0.0015 0.0029 0.0019 0.0011
Vacuole Periphery 0.0024 0.0005 0.0007 0.0001 0.0001 0.0018 0.0041 0.002 0.0004 0.0053 0.0045 0.0006 0.0018 0.0007 0.0004 0.0008 0.0015 0.0007

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 16.8666 5.8365 9.3838 11.6337 19.0466 9.7881 7.1308 7.6341 7.9699 12.0333
Translational Efficiency 1.2252 2.4897 1.4081 0.9169 0.7514 1.3378 1.6277 1.2917 0.9287 0.9425

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✔
Translational Efficiency ✔

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
414 869 1097 241 1408 2079 247 1246 1822 2948 1344 1487

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 759.30 737.85 891.22 949.09 720.59 803.86 960.12 1014.10 729.39 784.40 903.88 1003.56
Standard Deviation 96.74 106.89 127.28 156.00 89.73 110.11 116.23 155.75 92.80 113.24 128.13 157.62
Intensity Change Log 2 — -0.041342 0.231112 0.321875 — 0.157766 0.414036 0.492949 — 0.059041 0.323080 0.407710

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000290 0.000798 0.000711 0.001020 0.000153 0.000608 0.000687 0.000862 0.000184 0.000664 0.000707 0.000888
Bud Neck 0.002359 0.015985 0.019461 0.032800 0.004544 0.008235 0.005848 0.044732 0.004047 0.010520 0.016959 0.042798
Bud Site 0.000286 0.004847 0.001331 0.004506 0.000459 0.002000 0.000750 0.009221 0.000420 0.002839 0.001224 0.008456
Cell Periphery 0.000076 0.000232 0.000052 0.000138 0.000079 0.000097 0.000046 0.000162 0.000078 0.000137 0.000051 0.000158
Cytoplasm 0.004880 0.023994 0.002432 0.011324 0.010794 0.005124 0.003133 0.008075 0.009450 0.010686 0.002561 0.008602
Cytoplasmic Foci 0.003725 0.008080 0.000156 0.000640 0.003568 0.003320 0.001146 0.004382 0.003604 0.004723 0.000338 0.003776
Eisosomes 0.000047 0.000083 0.000038 0.000053 0.000054 0.000058 0.000073 0.000055 0.000052 0.000066 0.000045 0.000054
Endoplasmic Reticulum 0.003260 0.007544 0.003610 0.004880 0.001946 0.004495 0.004179 0.003969 0.002245 0.005393 0.003715 0.004117
Endosome 0.001266 0.007653 0.000851 0.002870 0.001857 0.004749 0.000702 0.007511 0.001723 0.005605 0.000823 0.006758
Golgi 0.001581 0.002917 0.000373 0.000839 0.000610 0.001431 0.000293 0.005112 0.000831 0.001869 0.000359 0.004420
Lipid Particles 0.003288 0.003947 0.000400 0.000592 0.003630 0.003041 0.000921 0.000465 0.003552 0.003308 0.000496 0.000485
Mitochondria 0.003832 0.012670 0.001725 0.003762 0.003833 0.009913 0.001057 0.007971 0.003833 0.010726 0.001602 0.007289
Mitotic Spindle 0.005180 0.025192 0.011987 0.097935 0.004983 0.010794 0.006847 0.064631 0.005028 0.015039 0.011042 0.070028
None 0.004016 0.021158 0.002298 0.005760 0.010624 0.004227 0.007305 0.004650 0.009123 0.009218 0.003218 0.004830
Nuclear Periphery 0.013150 0.008669 0.002884 0.013091 0.004544 0.010647 0.001452 0.013262 0.006500 0.010064 0.002621 0.013234
Nuclear Periphery Foci 0.010683 0.028035 0.006675 0.014865 0.019723 0.017021 0.004506 0.010591 0.017669 0.020268 0.006277 0.011284
Nucleolus 0.347393 0.325832 0.404667 0.379677 0.515710 0.405804 0.332854 0.419814 0.477465 0.382230 0.391469 0.413309
Nucleus 0.590121 0.486573 0.537034 0.410651 0.410002 0.500264 0.621716 0.370108 0.450929 0.496228 0.552597 0.376679
Peroxisomes 0.002898 0.005301 0.001062 0.002168 0.001649 0.002397 0.002120 0.002298 0.001933 0.003253 0.001256 0.002277
Vacuole 0.001204 0.006891 0.001637 0.008262 0.000759 0.003532 0.003958 0.013937 0.000860 0.004522 0.002064 0.013017
Vacuole Periphery 0.000464 0.003599 0.000615 0.004167 0.000479 0.002244 0.000407 0.008193 0.000475 0.002643 0.000577 0.007540

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -8.14 -7.94 -8.20 -3.18 -2.92 -1.75 -5.50 -13.16 -2.04 -1.87 -2.64 -12.58 -14.66 -2.51 -3.79
Bud Neck -9.61 -16.47 -9.66 -6.24 -4.61 -5.44 -3.27 -22.86 -21.13 -20.60 -10.15 -15.79 -24.79 -21.34 -15.56
Bud Site -6.24 -8.54 -6.21 -1.73 -4.34 -2.71 -3.73 -10.41 -8.53 -9.57 -5.38 -8.58 -11.29 -8.45 -9.63
Cell Periphery -3.17 0.53 -1.69 2.21 -4.25 -0.72 1.69 -3.73 -1.95 -5.15 -2.64 1.62 -3.95 -0.67 -6.04
Cytoplasm -5.49 1.60 -1.90 2.79 -3.05 3.28 4.50 -0.04 -3.09 -4.23 -0.75 5.63 -0.83 -0.19 -5.76
Cytoplasmic Foci -2.59 3.08 2.58 5.97 -2.95 0.81 4.58 1.25 0.56 -2.56 -1.17 7.18 1.83 2.93 -4.09
Eisosomes -6.59 1.61 -1.13 5.96 -3.41 -1.00 -4.40 -0.87 0.13 3.94 -4.74 2.26 -1.39 3.29 -3.66
Endoplasmic Reticulum -6.34 -1.44 -2.32 2.59 -1.66 -8.75 -2.71 -6.36 0.24 0.68 -11.05 -5.79 -6.51 2.24 -0.83
Endosome -5.23 0.95 -3.37 3.30 -4.33 -4.56 3.20 -5.17 -1.25 -6.49 -6.73 3.80 -5.70 0.09 -7.13
Golgi -1.70 1.69 1.01 3.67 -3.25 -2.46 3.21 -3.38 -2.48 -3.85 -3.25 2.82 -3.10 -1.73 -3.85
Lipid Particles -0.56 3.35 2.91 4.82 -1.70 0.88 4.71 5.88 4.96 2.83 0.51 6.64 6.60 6.74 -0.30
Mitochondria -4.50 1.65 -0.11 5.23 -3.78 -5.29 4.81 -3.40 2.43 -7.80 -6.98 4.23 -3.30 4.08 -7.64
Mitotic Spindle -5.07 -3.52 -6.09 -4.69 -5.34 -2.76 -1.15 -13.97 -13.02 -9.76 -5.49 -4.46 -15.21 -13.27 -12.60
None -7.42 3.24 -1.26 6.06 -2.69 5.01 1.69 4.45 -0.24 1.00 0.09 5.73 4.03 4.63 -1.82
Nuclear Periphery 1.39 3.37 0.30 -0.94 -2.76 -4.97 3.76 -4.25 -0.30 -6.56 -2.99 4.05 -3.08 -0.80 -6.40
Nuclear Periphery Foci -4.54 1.36 -0.81 3.35 -1.94 1.32 6.73 4.95 4.36 -2.44 -0.95 5.69 4.43 6.04 -1.42
Nucleolus 1.53 -3.69 0.00 -1.17 2.84 11.57 10.00 12.70 3.02 -2.27 11.60 8.64 10.73 1.20 1.86
Nucleus 6.54 4.20 8.00 3.51 5.65 -9.90 -10.95 3.91 14.35 13.18 -5.67 -9.02 7.81 14.52 16.33
Peroxisomes -1.75 1.39 0.52 3.46 -3.96 -1.78 -1.37 -3.05 -1.20 -1.29 -2.77 2.11 -1.81 1.40 -5.66
Vacuole -4.30 -1.33 -4.27 -2.02 -3.93 -5.19 -3.35 -9.61 -7.85 -5.16 -6.97 -4.52 -10.32 -7.83 -8.65
Vacuole Periphery -6.64 -1.69 -4.95 -0.34 -4.59 -4.82 -0.14 -7.27 -4.54 -7.25 -7.36 -1.94 -7.98 -4.35 -7.66
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Nucleolar S-adenosylmethionine-dependent rRNA methyltransferase; methylates adenine (m1A) of the large subunit (LSU) rRNA at position 2142; belongs to Rossmann fold superfamily; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YBR141C is not an essential gene
Localization
Cell Percentages nucleolus (43%), nucleus (23%), mixed (28%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Bmt2

Bmt2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Bmt2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available