Standard name
Description NADP+ dependent arabinose dehydrogenase; involved in carbohydrate metabolism; purified as homodimer; naturally occurs with a N-terminus degradation product
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.05 0.05 0.1
Bud Neck 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 – 0 0 0 0 0 0 0 0 0.05 0 0 0 0.06 0 0 0 0 0 0 0 0
Cytoplasm 0.94 0.96 – 1.0 0.94 0.84 0.83 0.75 0.77 0.7 0.75 0.8 0.99 0.96 0.97 0.97 0.97 0.97 0.87 0.93 0.87 0.87 0.87 0.78
Endoplasmic Reticulum 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Endosome 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.08 0 – 0 0 0.1 0.07 0.07 0.17 0.1 0.18 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 – 0 0.05 0.09 0.1 0.14 0.1 0.18 0.09 0.11 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 1 3 1 4 3 5 2 5 0 1 1 0 0 0 1 0 0 0 0 0
Bud 1 0 – 0 2 1 5 2 4 8 6 6 0 2 2 0 0 0 4 3 0 8 9 23
Bud Neck 0 0 – 0 2 1 0 1 1 1 1 1 0 0 0 0 0 0 1 0 0 1 0 1
Bud Site 0 0 – 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 – 1 6 1 6 4 7 8 5 14 1 0 0 15 4 10 0 0 0 0 1 1
Cytoplasm 232 72 – 143 314 246 319 244 236 247 168 215 123 175 175 258 145 250 217 77 32 150 168 174
Endoplasmic Reticulum 3 1 – 0 2 3 7 10 1 11 5 4 0 1 0 3 5 15 0 0 0 0 1 2
Endosome 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 1
Golgi 2 0 – 0 1 0 0 0 0 0 0 1 0 0 0 1 0 0 3 0 0 0 0 2
Mitochondria 20 1 – 2 3 29 26 23 52 35 40 12 0 0 0 0 0 1 8 0 0 2 2 4
Nucleus 1 0 – 0 1 3 6 8 10 4 5 3 0 1 1 1 0 1 0 0 0 1 0 0
Nuclear Periphery 0 1 – 0 0 2 4 1 4 3 1 1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 – 0 0 0 2 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0 1 0 1 2 1 1 0 1 0 0 0 0 0 0 3 0 0 1 3 4
Vac/Vac Membrane 0 0 – 0 16 25 39 46 32 63 20 30 0 5 5 4 2 2 1 0 1 4 2 2
Unique Cell Count 248 75 143 335 292 386 326 307 351 224 269 124 182 181 266 149 259 249 83 38 174 195 224
Labelled Cell Count 259 75 146 349 314 416 347 351 386 255 293 124 185 184 282 156 279 249 83 38 174 195 224


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.5 7.2 – 5.0 7.8 7.8 9.3 11.4 10.8 12.1 12.4 14.9 6.1 9.2 9.7 28.6 30.5 30.4 10.2 7.7 10.9
Std Deviation (1e-4) 1.6 2.9 – 1.3 3.5 4.4 5.2 6.0 5.9 6.5 6.0 7.5 2.0 5.7 5.6 6.9 8.3 9.8 12.1 6.4 10.5
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –