Standard name
Human Ortholog
Description Subunit of the conserved chromosomal passenger complex (CPC); complex regulates kinetochore-microtubule attachments, activation of the spindle tension checkpoint, and mitotic spindle disassembly; other complex members are Ipl1p, Bir1p, and Nbl1p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0.08 0 0.09 0 0 0.06 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0.06 0.05 0 0.06 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.1 0.07 0.1 0.07 0.07 0.07 0.07 0 0.06 0.09 0.05 0.05 0.14 0.17 0.09 0.12 0.07 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.18 0 0 0 0 0 0 0 0 0 0 0 0.23 0.4 0.36 0.24 0.21 0.28 0.11 0.13 0.12 0.1 0.1 0.09
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.24 0.51 0 0.08 0 0.25 0.27 0.14 0.57 0.49 0.5 0.28 0.14 0 0 0.08 0.1 0.13 0 0.06 0.07 0.08 0 0
Nucleus 0.12 0.11 0.19 0.18 0.18 0.2 0.16 0.16 0.18 0.13 0.12 0.11 0.16 0.13 0.14 0.15 0.18 0.18 0.07 0.06 0.08 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.34 0.08 0 0.09 0.14 0.1 0.17 0.15 0.18 0.18 0.2 0.13 0.26 0.23 0.2 0.42 0.4 0.39 0.08 0.12 0.13 0.08 0.07 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.09 0 0 0 0 0 0 0 0 0
SpindlePole 0.54 0.35 0.68 0.67 0.75 0.55 0.6 0.64 0.45 0.45 0.4 0.61 0.49 0.41 0.39 0.4 0.44 0.48 0.55 0.41 0.43 0.52 0.61 0.63
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0.06 0.07 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 4 0 2 0 1 2 2 0 3 1 0 0 0 0 0 0 0 1 0 0 2 1
Bud 2 5 2 1 3 8 14 22 17 24 32 16 1 0 0 1 1 0 1 3 3 1 5 5
Bud Neck 1 2 27 9 39 13 24 37 15 11 10 28 0 1 0 0 1 1 1 2 2 2 4 5
Bud Site 6 3 2 6 10 22 22 17 27 27 19 26 4 2 0 1 1 0 – – – – – –
Cell Periphery 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Cytoplasm 8 25 24 33 30 36 43 43 16 33 46 21 15 66 75 11 8 9 3 10 6 2 3 4
Endoplasmic Reticulum 1 2 2 1 1 1 1 1 0 0 0 0 0 0 0 2 2 3 0 0 0 0 0 0
Endosome 60 6 3 11 4 21 5 9 0 9 6 12 63 182 158 29 14 36 45 41 51 19 36 29
Golgi 0 0 1 1 0 0 1 0 0 0 0 0 0 0 2 0 0 0 1 0 0 0 0 0
Mitochondria 81 126 14 28 15 128 165 87 259 263 268 131 39 20 18 10 7 17 10 19 28 15 11 13
Nucleus 39 28 64 60 80 103 100 98 84 70 66 49 43 62 61 18 12 24 29 17 36 7 0 0
Nuclear Periphery 7 1 2 0 0 2 2 2 0 5 3 0 3 1 0 0 0 1 2 2 1 0 1 1
Nucleolus 114 21 13 29 63 53 106 88 80 98 107 59 72 104 87 51 27 51 32 37 55 15 24 13
Peroxisomes 6 0 1 1 0 3 4 0 0 3 1 1 10 34 39 0 1 0 0 2 2 3 7 8
SpindlePole 178 87 226 226 342 282 366 386 205 242 216 281 135 189 172 48 30 62 216 131 188 100 216 211
Vac/Vac Membrane 10 5 5 3 2 2 2 7 3 3 4 2 4 14 18 13 4 9 9 13 11 5 5 8
Unique Cell Count 331 248 333 336 456 513 613 600 457 542 540 461 273 460 443 120 68 130 395 317 443 194 355 337
Labelled Cell Count 513 311 391 409 591 674 856 799 708 788 781 627 389 675 631 184 108 213 395 317 443 194 355 337


Microtubule, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.3 3.5 4.9 4.5 5.1 4.1 4.3 4.5 3.6 3.5 3.4 3.9 4.3 4.5 4.6 7.8 7.7 7.5 4.3 4.3 4.7
Std Deviation (1e-4) 0.5 0.7 0.8 1.1 1.1 1.1 1.3 1.0 1.0 0.8 0.9 0.7 0.8 1.1 1.2 1.6 1.8 1.6 1.5 1.1 1.4
Intensity Change (Log2) – – – -0.13 0.06 -0.28 -0.21 -0.14 -0.45 -0.49 -0.53 -0.33 -0.19 -0.14 -0.09 0.66 0.64 0.61 -0.2 -0.2 -0.06


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.6648 -0.2069 -0.4701 -0.272 -0.7705 -0.2254 0.1467 -0.0024 0.0542 -0.1267 -0.1808 -0.2095 -1.0081 -0.4914 -0.7066 -0.0892 -0.3756 -0.8193
Actin 0.0048 0.001 0.0046 0.0067 0.0043 0.0005 0.0341 0.0002 0.0087 0.002 0.0045 0.003 0.0269 0.0005 0.0099 0.0152 0.0148 0.0028
Bud 0.0001 0 0 0 0.0001 0 0.0006 0.0001 0.0002 0.0003 0.0002 0.0001 0.0002 0.0002 0.0002 0.0003 0.0004 0.0001
Bud Neck 0.001 0.0009 0.0006 0.0009 0.0172 0.0027 0.0103 0.0014 0.001 0.0008 0.0303 0.0087 0.0008 0.0011 0.0021 0.0036 0.0043 0.0171
Bud Periphery 0.0001 0.0001 0.0001 0 0.0002 0.0001 0.0009 0.0001 0.0002 0.0003 0.0006 0.0005 0.0003 0.0005 0.0003 0.0003 0.0007 0.0005
Bud Site 0.0014 0.0006 0.0002 0.0001 0.0004 0.0001 0.008 0.0069 0.0013 0.001 0.0008 0.0005 0.0007 0.0022 0.0022 0.0013 0.002 0.0004
Cell Periphery 0 0 0 0 0.0001 0.0001 0.0004 0.0001 0 0 0.0001 0.0002 0.0001 0.0001 0.0001 0.0001 0.0003 0.0003
Cytoplasm 0.004 0.0044 0.0029 0.0068 0.0029 0.0014 0.0109 0.0071 0.0038 0.0023 0.0127 0.002 0.029 0.0116 0.0087 0.0257 0.0294 0.0015
Cytoplasmic Foci 0.0187 0.0092 0.0042 0.0025 0.0083 0.0091 0.0551 0.0162 0.0119 0.019 0.0263 0.0313 0.0689 0.0464 0.0208 0.026 0.0144 0.0679
Eisosomes 0.0001 0 0.0001 0.0001 0.0001 0.0001 0.0004 0 0.0001 0 0.0005 0.0002 0.0002 0 0.0002 0.0001 0.0004 0.0005
Endoplasmic Reticulum 0.0006 0.0003 0.0005 0.0001 0.0007 0.0003 0.0021 0.0005 0.0003 0.0001 0.0007 0.0006 0.004 0.0013 0.0003 0.0009 0.0035 0.0002
Endosome 0.0051 0.0028 0.0012 0.0008 0.005 0.0039 0.0289 0.0049 0.0029 0.0055 0.0133 0.0071 0.0121 0.0066 0.0035 0.0081 0.0098 0.0069
Golgi 0.0018 0.0006 0.001 0.0008 0.0016 0.0011 0.0107 0.0009 0.0015 0.0089 0.0067 0.0043 0.0042 0.002 0.0021 0.0056 0.008 0.0043
Lipid Particles 0.0187 0.0141 0.0094 0.0022 0.0247 0.0306 0.0517 0.0126 0.0098 0.0048 0.1066 0.0745 0.042 0.0316 0.0179 0.0146 0.0734 0.1252
Mitochondria 0.001 0.0002 0.0005 0.0004 0.0008 0.0059 0.0228 0.0003 0.0007 0.0008 0.0218 0.0311 0.0013 0.0219 0.0062 0.001 0.0199 0.0393
None 0.0237 0.0155 0.007 0.0681 0.0075 0.0137 0.007 0.0168 0.0113 0.0014 0.0317 0.0122 0.0257 0.0164 0.0554 0.0873 0.0464 0.0291
Nuclear Periphery 0.0221 0.016 0.0081 0.0136 0.0411 0.0152 0.0361 0.0089 0.011 0.0093 0.02 0.012 0.0194 0.0103 0.0067 0.0356 0.0662 0.0063
Nucleolus 0.0272 0.0532 0.0399 0.0353 0.0397 0.0416 0.0154 0.0512 0.0311 0.0206 0.0212 0.0651 0.0061 0.0281 0.0237 0.0301 0.0387 0.036
Nucleus 0.0411 0.1326 0.2516 0.4009 0.0888 0.0618 0.0334 0.1417 0.1967 0.4902 0.0486 0.0369 0.0171 0.1335 0.2347 0.3569 0.1185 0.0379
Peroxisomes 0.0063 0.0033 0.002 0.0258 0.0076 0.0058 0.0149 0.0011 0.0155 0.0095 0.0216 0.0223 0.0097 0.0101 0.0027 0.0136 0.0212 0.0341
Punctate Nuclear 0.8215 0.7444 0.6657 0.4347 0.7475 0.8046 0.6524 0.7281 0.6916 0.4231 0.6291 0.6853 0.7299 0.6738 0.6013 0.3728 0.5244 0.5873
Vacuole 0.0004 0.0004 0.0002 0.0001 0.0007 0.0008 0.0027 0.0008 0.0003 0.0002 0.001 0.001 0.0008 0.0012 0.0006 0.0006 0.0021 0.0011
Vacuole Periphery 0.0003 0.0002 0.0001 0.0001 0.0006 0.0007 0.0013 0.0001 0.0001 0.0001 0.0016 0.0009 0.0005 0.0004 0.0004 0.0004 0.0015 0.0011

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 14.7331 7.0556 5.2356 6.8546 11.9388 9.466 9.1552 7.8644 5.6132 13.9033
Translational Efficiency 1.0071 1.6656 0.8076 0.5819 1.0124 1.495 0.8815 0.7032 0.9495 0.7687

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✔
Gene Expression ✔
Translational Efficiency ✘