Standard name | |
---|---|
Human Ortholog | |
Description | Modulator of cell separation and mitotic exit; inhibits separation through Ub-dependent Ace2p proteolysis; part of a daughter-specific switch induced by the mitotic exit network that inhibits exit and resets the cell cycle after the execution of MEN function, blocking Tem1p and Cdc15 association; required for chromosome stability and multiple mitotic checkpoints; regulated by SCF; haploid transcription regulated by Ste12p; contains 12 degenerate leucine-rich repeat motifs and an atypical F-box |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 5 | 12 | 0 | 3 | 8 |
Bud | 3 | 1 | 3 | 0 | 2 | 10 | 7 | 9 | 10 | 11 | 6 | 4 | 0 | 2 | 0 | – | 0 | 0 | 6 | 20 | 8 | 2 | 5 | 10 |
Bud Neck | 0 | 1 | 1 | 1 | 3 | 3 | 4 | 8 | 1 | 3 | 0 | 2 | 0 | 0 | 0 | – | 2 | 3 | 0 | 2 | 6 | 0 | 1 | 4 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 5 | 2 | 2 | 2 | 0 | 0 | 0 | – | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 1 | 1 | 2 | 1 | 0 | 0 | 2 | 0 | 0 | 0 | 2 | 1 | 0 | 1 | – | 11 | 6 | 0 | 2 | 2 | 1 | 1 | 3 |
Cytoplasm | 102 | 366 | 395 | 222 | 263 | 310 | 351 | 298 | 240 | 279 | 78 | 74 | 266 | 338 | 364 | – | 525 | 507 | 84 | 260 | 251 | 136 | 221 | 217 |
Endoplasmic Reticulum | 0 | 0 | 1 | 0 | 2 | 0 | 0 | 1 | 0 | 0 | 5 | 6 | 2 | 0 | 4 | – | 49 | 42 | 1 | 1 | 4 | 1 | 3 | 5 |
Endosome | 3 | 3 | 1 | 0 | 3 | 2 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 3 | 0 | – | 6 | 5 | 1 | 4 | 14 | 0 | 3 | 4 |
Golgi | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 1 | 1 | 1 | 3 | 0 |
Mitochondria | 24 | 11 | 13 | 29 | 43 | 190 | 235 | 179 | 265 | 317 | 152 | 221 | 0 | 2 | 0 | – | 10 | 10 | 13 | 28 | 89 | 8 | 13 | 21 |
Nucleus | 17 | 39 | 3 | 0 | 2 | 2 | 3 | 2 | 3 | 2 | 1 | 1 | 4 | 0 | 0 | – | 1 | 2 | 29 | 51 | 5 | 2 | 10 | 6 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 2 | 1 | 1 | 1 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 2 | 0 | 1 | 1 | 0 | 0 | 0 |
SpindlePole | 1 | 0 | 2 | 0 | 1 | 5 | 8 | 13 | 7 | 8 | 2 | 5 | 0 | 0 | 0 | – | 4 | 1 | 22 | 32 | 12 | 4 | 12 | 10 |
Vac/Vac Membrane | 20 | 60 | 19 | 1 | 3 | 5 | 8 | 6 | 5 | 5 | 1 | 3 | 5 | 0 | 1 | – | 35 | 22 | 13 | 43 | 20 | 6 | 14 | 28 |
Unique Cell Count | 153 | 450 | 419 | 230 | 298 | 426 | 514 | 446 | 417 | 491 | 204 | 264 | 274 | 342 | 369 | 553 | 534 | 182 | 465 | 446 | 171 | 304 | 333 | |
Labelled Cell Count | 173 | 482 | 440 | 255 | 326 | 531 | 616 | 519 | 537 | 631 | 248 | 322 | 279 | 345 | 370 | 645 | 601 | 182 | 465 | 446 | 171 | 304 | 333 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.9 | 4.3 | 4.0 | 3.5 | 3.8 | 3.2 | 2.6 | 3.0 | 2.9 | 2.5 | 2.7 | 2.5 | 4.5 | 4.2 | 4.4 | 4.8 | 5.9 | 5.4 | 4.3 | 4.5 | 5.0 |
Std Deviation (1e-4) | 1.4 | 0.8 | 1.6 | 1.5 | 1.8 | 1.4 | 0.7 | 1.0 | 1.1 | 0.7 | 0.6 | 0.7 | 1.4 | 1.2 | 1.6 | 1.9 | 1.9 | 2.0 | 1.9 | 1.9 | 1.5 |
Intensity Change (Log2) | – | – | – | -0.18 | -0.07 | -0.31 | -0.63 | -0.43 | -0.47 | -0.66 | -0.53 | -0.65 | 0.16 | 0.07 | 0.14 | 0.28 | 0.57 | 0.45 | 0.1 | 0.17 | 0.32 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | 1.2 | -0.3 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.1693 | 1.2017 | 1.3169 | 1.1106 | 0.343 | 1.8065 | 2.9311 | 2.3238 | 2.4789 | 2.6638 | 2.0554 | 2.406 | 4.2038 | 2.9067 | 3.1727 | 2.9359 | 3.3377 | 3.1537 |
Actin | 0.1846 | 0.0009 | 0.0001 | 0.0011 | 0.0041 | 0.0016 | 0.0059 | 0.0029 | 0.0287 | 0.0008 | 0.003 | 0.0052 | 0.0109 | 0.0005 | 0.0146 | 0.0004 | 0.003 | 0.0019 |
Bud | 0.007 | 0.0004 | 0 | 0.0062 | 0.0018 | 0.0058 | 0.0004 | 0.017 | 0.0156 | 0.0002 | 0.0002 | 0.0018 | 0.0005 | 0.0066 | 0.0036 | 0.0004 | 0.007 | 0.0015 |
Bud Neck | 0.0043 | 0.0001 | 0 | 0.0001 | 0.0002 | 0.0067 | 0.0008 | 0.0008 | 0.0006 | 0 | 0.0001 | 0.0015 | 0.0088 | 0.0005 | 0.0026 | 0.0005 | 0.0001 | 0.0039 |
Bud Periphery | 0.0344 | 0.0009 | 0.0001 | 0.0044 | 0.0038 | 0.0072 | 0.0005 | 0.0147 | 0.0044 | 0.0007 | 0.0003 | 0.001 | 0.0008 | 0.0079 | 0.0087 | 0.0004 | 0.0104 | 0.0019 |
Bud Site | 0.0184 | 0.0013 | 0.0001 | 0.0019 | 0.001 | 0.0093 | 0.0027 | 0.0263 | 0.0112 | 0 | 0.0006 | 0.0003 | 0.0067 | 0.0151 | 0.0092 | 0.0007 | 0.0009 | 0.0006 |
Cell Periphery | 0.0052 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0003 | 0.0002 | 0.001 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0005 | 0.0004 | 0.0007 | 0.0001 | 0.0003 | 0.0003 |
Cytoplasm | 0.1914 | 0.0986 | 0.0315 | 0.0896 | 0.017 | 0.1474 | 0.2142 | 0.1554 | 0.1082 | 0.0399 | 0.1542 | 0.2222 | 0.1769 | 0.0819 | 0.0854 | 0.0933 | 0.0597 | 0.2033 |
Cytoplasmic Foci | 0.0198 | 0.0024 | 0.0018 | 0.0049 | 0.0083 | 0.0101 | 0.0607 | 0.0051 | 0.0171 | 0.0036 | 0.0049 | 0.0137 | 0.0326 | 0.0069 | 0.0106 | 0.0425 | 0.0075 | 0.0136 |
Eisosomes | 0.0019 | 0.0001 | 0.0002 | 0.0004 | 0.0004 | 0.0001 | 0.0004 | 0.0003 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0004 | 0.0002 | 0.0003 | 0.0002 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0216 | 0.0014 | 0.0004 | 0.0011 | 0.0003 | 0.002 | 0.0043 | 0.0038 | 0.0019 | 0.0006 | 0.0009 | 0.0021 | 0.0102 | 0.0023 | 0.0044 | 0.0013 | 0.001 | 0.0022 |
Endosome | 0.0236 | 0.0005 | 0.0002 | 0.0005 | 0.0002 | 0.0015 | 0.0296 | 0.0077 | 0.0035 | 0.0004 | 0.0179 | 0.0051 | 0.0223 | 0.0015 | 0.0142 | 0.0161 | 0.0018 | 0.0027 |
Golgi | 0.011 | 0.0001 | 0 | 0.0002 | 0.0002 | 0.0002 | 0.0158 | 0.0022 | 0.0029 | 0.0001 | 0.0224 | 0.004 | 0.0036 | 0.0001 | 0.0075 | 0.0016 | 0.0047 | 0.0003 |
Lipid Particles | 0.0131 | 0.0002 | 0.0002 | 0.0003 | 0.0007 | 0.0004 | 0.0171 | 0.0005 | 0.0084 | 0.0003 | 0.0009 | 0.0026 | 0.0156 | 0.0003 | 0.0041 | 0.0019 | 0.0097 | 0.0008 |
Mitochondria | 0.0299 | 0.0002 | 0.0002 | 0.0009 | 0.001 | 0.0011 | 0.0083 | 0.0036 | 0.0129 | 0.0004 | 0.0138 | 0.0022 | 0.0018 | 0.0007 | 0.0043 | 0.0005 | 0.0289 | 0.0009 |
None | 0.3011 | 0.8906 | 0.9638 | 0.8622 | 0.9449 | 0.5756 | 0.4482 | 0.7307 | 0.7491 | 0.9475 | 0.778 | 0.6439 | 0.5325 | 0.8468 | 0.795 | 0.8285 | 0.8305 | 0.6766 |
Nuclear Periphery | 0.0171 | 0.0004 | 0.0002 | 0.0024 | 0.0007 | 0.0039 | 0.0091 | 0.0027 | 0.0009 | 0.0007 | 0.0003 | 0.0067 | 0.0097 | 0.002 | 0.0083 | 0.0006 | 0.0002 | 0.003 |
Nucleolus | 0.007 | 0.0001 | 0.0001 | 0.001 | 0.0012 | 0.003 | 0.0017 | 0.0009 | 0.0007 | 0.0003 | 0.0001 | 0.0013 | 0.0014 | 0.0009 | 0.0006 | 0.0004 | 0.0001 | 0.0011 |
Nucleus | 0.0117 | 0.0005 | 0.0002 | 0.0058 | 0.0016 | 0.199 | 0.1373 | 0.0162 | 0.0072 | 0.0005 | 0.0003 | 0.0642 | 0.1401 | 0.0169 | 0.0118 | 0.0009 | 0.0003 | 0.0714 |
Peroxisomes | 0.0107 | 0.0001 | 0.0001 | 0.0005 | 0.0014 | 0.0002 | 0.03 | 0.0004 | 0.0227 | 0.0002 | 0.0005 | 0.0031 | 0.0099 | 0.0004 | 0.0043 | 0.0017 | 0.033 | 0.0008 |
Punctate Nuclear | 0.0298 | 0.0003 | 0.0003 | 0.0157 | 0.0106 | 0.0213 | 0.0095 | 0.0037 | 0.0015 | 0.0027 | 0.0003 | 0.0173 | 0.012 | 0.0053 | 0.0019 | 0.0054 | 0.0003 | 0.011 |
Vacuole | 0.0448 | 0.0005 | 0.0002 | 0.0005 | 0.0002 | 0.0029 | 0.0027 | 0.003 | 0.0013 | 0.0006 | 0.0007 | 0.0012 | 0.0023 | 0.0026 | 0.0056 | 0.0022 | 0.0003 | 0.0019 |
Vacuole Periphery | 0.0115 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0004 | 0.0007 | 0.001 | 0.0004 | 0.0003 | 0.0005 | 0.0005 | 0.0007 | 0.0003 | 0.0023 | 0.0002 | 0.0001 | 0.0004 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 15.7152 | 14.3473 | 39.9237 | 153.9837 | 119.6512 | 15.3723 | 15.111 | 16.9301 | 85.2862 | 155.6867 |
Translational Efficiency | 0.1324 | 0.1742 | 0.2881 | 0.2099 | 0.3775 | 0.1662 | 0.1473 | 0.1551 | 0.2286 | 0.3435 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1186 | 1454 | 282 | 1660 | 1183 | 2062 | 245 | 3050 | 2369 | 3516 | 527 | 4710 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 629.18 | 688.34 | 867.28 | 884.58 | 623.88 | 720.09 | 930.68 | 987.58 | 626.53 | 706.96 | 896.75 | 951.28 |
Standard Deviation | 65.48 | 85.65 | 119.44 | 153.76 | 72.74 | 104.81 | 119.42 | 394.01 | 69.25 | 98.59 | 123.54 | 333.59 |
Intensity Change Log 2 | — | 0.129649 | 0.463025 | 0.491520 | — | 0.206909 | 0.577017 | 0.662629 | — | 0.168632 | 0.520905 | 0.579248 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000048 | 0.000380 | 0.000385 | 0.000833 | 0.000168 | 0.000356 | 0.000643 | 0.000782 | 0.000108 | 0.000366 | 0.000505 | 0.000800 |
Bud Neck | 0.010311 | 0.027912 | 0.003054 | 0.022081 | 0.010718 | 0.037548 | 0.008183 | 0.023920 | 0.010514 | 0.033563 | 0.005438 | 0.023272 |
Bud Site | 0.002686 | 0.014951 | 0.001869 | 0.034174 | 0.003397 | 0.017101 | 0.010523 | 0.036993 | 0.003041 | 0.016212 | 0.005892 | 0.035999 |
Cell Periphery | 0.000161 | 0.000186 | 0.000176 | 0.000342 | 0.000166 | 0.000219 | 0.000275 | 0.000559 | 0.000163 | 0.000205 | 0.000222 | 0.000483 |
Cytoplasm | 0.537447* | 0.396285* | 0.120134* | 0.371780* | 0.440113* | 0.441059* | 0.188299* | 0.516101* | 0.488842* | 0.422543* | 0.151824* | 0.465236* |
Cytoplasmic Foci | 0.181026* | 0.170640 | 0.013129 | 0.012078 | 0.180131* | 0.145552 | 0.008873 | 0.003951 | 0.180579* | 0.155927 | 0.011150 | 0.006816 |
Eisosomes | 0.000158 | 0.000103 | 0.000071 | 0.000036 | 0.000139 | 0.000085 | 0.000082 | 0.000017 | 0.000149 | 0.000092 | 0.000076 | 0.000024 |
Endoplasmic Reticulum | 0.000399 | 0.001177 | 0.004823 | 0.003296 | 0.000675 | 0.000955 | 0.007255 | 0.000816 | 0.000537 | 0.001046 | 0.005954 | 0.001690 |
Endosome | 0.006595 | 0.014518 | 0.002681 | 0.005269 | 0.007606 | 0.015131 | 0.003318 | 0.002037 | 0.007100 | 0.014877 | 0.002977 | 0.003176 |
Golgi | 0.001171 | 0.004267 | 0.000072 | 0.002050 | 0.001683 | 0.003592 | 0.000115 | 0.001449 | 0.001427 | 0.003871 | 0.000092 | 0.001661 |
Lipid Particles | 0.007350 | 0.006376 | 0.002329 | 0.001022 | 0.010390 | 0.005475 | 0.001986 | 0.000328 | 0.008868 | 0.005847 | 0.002170 | 0.000572 |
Mitochondria | 0.002974 | 0.012447 | 0.001184 | 0.004023 | 0.005066 | 0.015640 | 0.000959 | 0.006098 | 0.004018 | 0.014319 | 0.001079 | 0.005366 |
Mitotic Spindle | 0.000768 | 0.005655 | 0.005629 | 0.076023 | 0.000867 | 0.013787 | 0.019566 | 0.062135 | 0.000818 | 0.010424 | 0.012108 | 0.067030 |
None | 0.005108 | 0.008990 | 0.005502 | 0.010452 | 0.005983 | 0.006960 | 0.006801 | 0.014377 | 0.005545 | 0.007799 | 0.006106 | 0.012994 |
Nuclear Periphery | 0.000374 | 0.000636 | 0.001728 | 0.002000 | 0.000765 | 0.000888 | 0.002051 | 0.001231 | 0.000569 | 0.000784 | 0.001878 | 0.001502 |
Nuclear Periphery Foci | 0.000214 | 0.000445 | 0.000744 | 0.002082 | 0.000314 | 0.000479 | 0.001655 | 0.000483 | 0.000264 | 0.000465 | 0.001168 | 0.001047 |
Nucleolus | 0.000996 | 0.004505 | 0.001376 | 0.001632 | 0.002302 | 0.003809 | 0.001513 | 0.001266 | 0.001648 | 0.004096 | 0.001439 | 0.001395 |
Nucleus | 0.204044* | 0.253333* | 0.781231* | 0.387264* | 0.290911* | 0.225629* | 0.628145* | 0.293512* | 0.247422* | 0.237085* | 0.710062* | 0.326554* |
Peroxisomes | 0.002130 | 0.012705 | 0.000324 | 0.001037 | 0.004721 | 0.010839 | 0.000299 | 0.000384 | 0.003424 | 0.011611 | 0.000312 | 0.000614 |
Vacuole | 0.035534 | 0.062576 | 0.053255 | 0.058380 | 0.032813 | 0.052943 | 0.109165 | 0.031892 | 0.034175 | 0.056927 | 0.079247 | 0.041228 |
Vacuole Periphery | 0.000508 | 0.001913 | 0.000306 | 0.004144 | 0.001071 | 0.001954 | 0.000295 | 0.001668 | 0.000789 | 0.001937 | 0.000301 | 0.002540 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -6.52 | -9.38 | -11.28 | -4.60 | -3.36 | -1.86 | -4.80 | -2.85 | -1.24 | 1.35 | -4.38 | -8.25 | -6.53 | -2.79 | -0.24 |
Bud Neck | -8.15 | 5.60 | -6.51 | 2.70 | -13.42 | -11.55 | 2.79 | -9.67 | 5.31 | -11.67 | -14.17 | 5.68 | -11.93 | 5.75 | -18.87 |
Bud Site | -6.94 | 0.69 | -9.83 | -2.93 | -10.57 | -7.74 | -1.53 | -11.15 | -3.34 | -6.14 | -10.63 | -1.18 | -15.26 | -4.51 | -11.84 |
Cell Periphery | -1.56 | -0.54 | -3.21 | -1.81 | -1.83 | -1.02 | -1.80 | -2.46 | -0.90 | -1.16 | -1.35 | -1.67 | -3.23 | -1.33 | -1.95 |
Cytoplasm | 14.01 | 35.95 | 27.32 | 12.16 | -14.71 | -0.18 | 18.63 | 4.52 | 5.18 | -16.93 | 9.27 | 37.32 | 19.80 | 10.42 | -24.60 |
Cytoplasmic Foci | 1.24 | 30.41 | 33.04 | 29.48 | 0.66 | 5.03 | 29.74 | 31.25 | 34.19 | 2.80 | 4.86 | 42.32 | 45.75 | 45.22 | 2.72 |
Eisosomes | 5.66 | 6.06 | 12.15 | 9.20 | 4.55 | 7.63 | 3.10 | 17.55 | 17.11 | 9.96 | 9.51 | 6.62 | 21.30 | 19.10 | 11.17 |
Endoplasmic Reticulum | -8.07 | -10.40 | -21.83 | -17.11 | 1.98 | -2.44 | -8.37 | -7.17 | -4.30 | 7.58 | -6.73 | -12.73 | -21.71 | -15.30 | 8.57 |
Endosome | -6.93 | 5.21 | 4.17 | 10.24 | -1.51 | -6.66 | 4.92 | 9.54 | 14.33 | 5.32 | -9.65 | 7.22 | 10.58 | 17.84 | 3.70 |
Golgi | -3.83 | 3.06 | 1.09 | 4.72 | -3.20 | -2.73 | 3.17 | 2.03 | 5.91 | -2.93 | -4.75 | 4.36 | 2.31 | 7.49 | -4.24 |
Lipid Particles | 0.95 | 5.63 | 7.47 | 8.77 | 3.26 | 5.30 | 9.72 | 12.74 | 9.44 | 6.97 | 4.56 | 10.85 | 14.69 | 12.98 | 7.87 |
Mitochondria | -5.67 | 2.02 | 0.59 | 6.90 | -2.64 | -6.12 | 4.38 | 1.43 | 8.04 | -5.37 | -8.47 | 4.51 | 1.20 | 10.40 | -5.55 |
Mitotic Spindle | -3.65 | -0.96 | -14.54 | -13.20 | -11.38 | -7.16 | -2.26 | -19.50 | -13.49 | -9.22 | -8.00 | -2.34 | -24.28 | -19.03 | -15.08 |
None | -2.92 | 0.60 | -4.27 | -0.13 | -5.74 | -0.83 | 1.56 | -8.85 | -9.88 | -12.46 | -2.65 | 1.54 | -10.47 | -7.59 | -14.18 |
Nuclear Periphery | -3.75 | -11.73 | -27.03 | -23.96 | -10.58 | -0.45 | -10.37 | -14.46 | -5.59 | 0.77 | -1.29 | -15.16 | -27.06 | -12.11 | -5.10 |
Nuclear Periphery Foci | -5.22 | -4.74 | -7.48 | -7.12 | -6.31 | -1.55 | -6.02 | -3.24 | -1.71 | 4.58 | -3.28 | -7.34 | -8.13 | -7.20 | -2.26 |
Nucleolus | -7.12 | -1.65 | -5.19 | 1.54 | -3.95 | -2.37 | 2.31 | -3.07 | -1.17 | -6.52 | -6.35 | 1.37 | -5.74 | -0.04 | -7.60 |
Nucleus | -5.36 | -34.25 | -22.83 | -16.12 | 20.08 | 7.08 | -12.81 | -11.09 | -20.91 | 8.42 | 1.68 | -30.29 | -23.94 | -26.13 | 19.50 |
Peroxisomes | -9.57 | 7.67 | 5.61 | 10.97 | -3.32 | -6.42 | 8.29 | 8.09 | 14.20 | -1.57 | -11.72 | 10.51 | 9.82 | 17.78 | -3.14 |
Vacuole | -8.59 | -4.68 | -19.14 | -12.63 | -5.84 | -7.17 | -9.71 | -10.15 | -3.42 | 7.86 | -11.06 | -10.33 | -20.46 | -10.95 | 4.41 |
Vacuole Periphery | -8.14 | 2.89 | -1.37 | 1.14 | -1.77 | -2.01 | 1.92 | 0.77 | 6.39 | -3.40 | -4.84 | 2.38 | -0.44 | 4.35 | -3.03 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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