Standard name
Human Ortholog
Description Modulator of cell separation and mitotic exit; inhibits separation through Ub-dependent Ace2p proteolysis; part of a daughter-specific switch induced by the mitotic exit network that inhibits exit and resets the cell cycle after the execution of MEN function, blocking Tem1p and Cdc15 association; required for chromosome stability and multiple mitotic checkpoints; regulated by SCF; haploid transcription regulated by Ste12p; contains 12 degenerate leucine-rich repeat motifs and an atypical F-box
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 0.67 0.81 0.94 0.97 0.88 0.73 0.68 0.67 0.58 0.57 0.38 0.28 0.97 0.99 0.99 – 0.95 0.95 0.46 0.56 0.56 0.8 0.73 0.65
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.09 0.08 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0.16 0 0 0.13 0.14 0.45 0.46 0.4 0.64 0.65 0.75 0.84 0 0 0 – 0 0 0.07 0.06 0.2 0.05 0 0.06
Nucleus 0.11 0.09 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0.16 0.11 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0.13 0.07 0 0 0 0
Vac/Vac Membrane 0.13 0.13 0.05 0 0 0 0 0 0 0 0 0 0 0 0 – 0.06 0 0.07 0.09 0.05 0 0.05 0.09
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 1 5 12 0 3 8
Bud 3 1 3 0 2 10 7 9 10 11 6 4 0 2 0 – 0 0 6 20 8 2 5 10
Bud Neck 0 1 1 1 3 3 4 8 1 3 0 2 0 0 0 – 2 3 0 2 6 0 1 4
Bud Site 0 0 0 0 0 2 0 0 5 2 2 2 0 0 0 – 0 0 – – – – – –
Cell Periphery 2 1 1 2 1 0 0 2 0 0 0 2 1 0 1 – 11 6 0 2 2 1 1 3
Cytoplasm 102 366 395 222 263 310 351 298 240 279 78 74 266 338 364 – 525 507 84 260 251 136 221 217
Endoplasmic Reticulum 0 0 1 0 2 0 0 1 0 0 5 6 2 0 4 – 49 42 1 1 4 1 3 5
Endosome 3 3 1 0 3 2 0 0 1 1 0 0 0 3 0 – 6 5 1 4 14 0 3 4
Golgi 0 0 1 0 1 1 0 0 0 0 0 0 0 0 0 – 1 0 0 1 1 1 3 0
Mitochondria 24 11 13 29 43 190 235 179 265 317 152 221 0 2 0 – 10 10 13 28 89 8 13 21
Nucleus 17 39 3 0 2 2 3 2 3 2 1 1 4 0 0 – 1 2 29 51 5 2 10 6
Nuclear Periphery 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 1 0 0 0 1 1 0 1 0 2 1 1 1 0 0 – 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 – 1 2 0 1 1 0 0 0
SpindlePole 1 0 2 0 1 5 8 13 7 8 2 5 0 0 0 – 4 1 22 32 12 4 12 10
Vac/Vac Membrane 20 60 19 1 3 5 8 6 5 5 1 3 5 0 1 – 35 22 13 43 20 6 14 28
Unique Cell Count 153 450 419 230 298 426 514 446 417 491 204 264 274 342 369 553 534 182 465 446 171 304 333
Labelled Cell Count 173 482 440 255 326 531 616 519 537 631 248 322 279 345 370 645 601 182 465 446 171 304 333


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.9 4.3 4.0 3.5 3.8 3.2 2.6 3.0 2.9 2.5 2.7 2.5 4.5 4.2 4.4 4.8 5.9 5.4 4.3 4.5 5.0
Std Deviation (1e-4) 1.4 0.8 1.6 1.5 1.8 1.4 0.7 1.0 1.1 0.7 0.6 0.7 1.4 1.2 1.6 1.9 1.9 2.0 1.9 1.9 1.5
Intensity Change (Log2) – – – -0.18 -0.07 -0.31 -0.63 -0.43 -0.47 -0.66 -0.53 -0.65 0.16 0.07 0.14 0.28 0.57 0.45 0.1 0.17 0.32


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – 0 0
Bud Neck – – – – – – – – – – – – – 0 0
Bud Site – – – – – – – – – – – – – 0 0
Cell Periphery – – – – – – – – – – – – – 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – 0 0
Endosome – – – – – – – – – – – – – 0 0
Golgi – – – – – – – – – – – – – 0 0
Mitochondria – – – – – – – – – – – – – 0 0
Nuclear Periphery – – – – – – – – – – – – – 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – 0 0
Peroxisomes – – – – – – – – – – – – – 0 0
SpindlePole – – – – – – – – – – – – – 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – 0 0
Cytoplasm – – – – – – – – – – – – – 0 0
Nucleus – – – – – – – – – – – – – 0 0
Vacuole – – – – – – – – – – – – – 1.2 -0.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.1693 1.2017 1.3169 1.1106 0.343 1.8065 2.9311 2.3238 2.4789 2.6638 2.0554 2.406 4.2038 2.9067 3.1727 2.9359 3.3377 3.1537
Actin 0.1846 0.0009 0.0001 0.0011 0.0041 0.0016 0.0059 0.0029 0.0287 0.0008 0.003 0.0052 0.0109 0.0005 0.0146 0.0004 0.003 0.0019
Bud 0.007 0.0004 0 0.0062 0.0018 0.0058 0.0004 0.017 0.0156 0.0002 0.0002 0.0018 0.0005 0.0066 0.0036 0.0004 0.007 0.0015
Bud Neck 0.0043 0.0001 0 0.0001 0.0002 0.0067 0.0008 0.0008 0.0006 0 0.0001 0.0015 0.0088 0.0005 0.0026 0.0005 0.0001 0.0039
Bud Periphery 0.0344 0.0009 0.0001 0.0044 0.0038 0.0072 0.0005 0.0147 0.0044 0.0007 0.0003 0.001 0.0008 0.0079 0.0087 0.0004 0.0104 0.0019
Bud Site 0.0184 0.0013 0.0001 0.0019 0.001 0.0093 0.0027 0.0263 0.0112 0 0.0006 0.0003 0.0067 0.0151 0.0092 0.0007 0.0009 0.0006
Cell Periphery 0.0052 0.0003 0.0002 0.0001 0.0001 0.0003 0.0002 0.001 0.0004 0.0001 0.0001 0.0001 0.0005 0.0004 0.0007 0.0001 0.0003 0.0003
Cytoplasm 0.1914 0.0986 0.0315 0.0896 0.017 0.1474 0.2142 0.1554 0.1082 0.0399 0.1542 0.2222 0.1769 0.0819 0.0854 0.0933 0.0597 0.2033
Cytoplasmic Foci 0.0198 0.0024 0.0018 0.0049 0.0083 0.0101 0.0607 0.0051 0.0171 0.0036 0.0049 0.0137 0.0326 0.0069 0.0106 0.0425 0.0075 0.0136
Eisosomes 0.0019 0.0001 0.0002 0.0004 0.0004 0.0001 0.0004 0.0003 0.0003 0.0002 0.0001 0.0001 0.0004 0.0002 0.0003 0.0002 0.0001 0.0001
Endoplasmic Reticulum 0.0216 0.0014 0.0004 0.0011 0.0003 0.002 0.0043 0.0038 0.0019 0.0006 0.0009 0.0021 0.0102 0.0023 0.0044 0.0013 0.001 0.0022
Endosome 0.0236 0.0005 0.0002 0.0005 0.0002 0.0015 0.0296 0.0077 0.0035 0.0004 0.0179 0.0051 0.0223 0.0015 0.0142 0.0161 0.0018 0.0027
Golgi 0.011 0.0001 0 0.0002 0.0002 0.0002 0.0158 0.0022 0.0029 0.0001 0.0224 0.004 0.0036 0.0001 0.0075 0.0016 0.0047 0.0003
Lipid Particles 0.0131 0.0002 0.0002 0.0003 0.0007 0.0004 0.0171 0.0005 0.0084 0.0003 0.0009 0.0026 0.0156 0.0003 0.0041 0.0019 0.0097 0.0008
Mitochondria 0.0299 0.0002 0.0002 0.0009 0.001 0.0011 0.0083 0.0036 0.0129 0.0004 0.0138 0.0022 0.0018 0.0007 0.0043 0.0005 0.0289 0.0009
None 0.3011 0.8906 0.9638 0.8622 0.9449 0.5756 0.4482 0.7307 0.7491 0.9475 0.778 0.6439 0.5325 0.8468 0.795 0.8285 0.8305 0.6766
Nuclear Periphery 0.0171 0.0004 0.0002 0.0024 0.0007 0.0039 0.0091 0.0027 0.0009 0.0007 0.0003 0.0067 0.0097 0.002 0.0083 0.0006 0.0002 0.003
Nucleolus 0.007 0.0001 0.0001 0.001 0.0012 0.003 0.0017 0.0009 0.0007 0.0003 0.0001 0.0013 0.0014 0.0009 0.0006 0.0004 0.0001 0.0011
Nucleus 0.0117 0.0005 0.0002 0.0058 0.0016 0.199 0.1373 0.0162 0.0072 0.0005 0.0003 0.0642 0.1401 0.0169 0.0118 0.0009 0.0003 0.0714
Peroxisomes 0.0107 0.0001 0.0001 0.0005 0.0014 0.0002 0.03 0.0004 0.0227 0.0002 0.0005 0.0031 0.0099 0.0004 0.0043 0.0017 0.033 0.0008
Punctate Nuclear 0.0298 0.0003 0.0003 0.0157 0.0106 0.0213 0.0095 0.0037 0.0015 0.0027 0.0003 0.0173 0.012 0.0053 0.0019 0.0054 0.0003 0.011
Vacuole 0.0448 0.0005 0.0002 0.0005 0.0002 0.0029 0.0027 0.003 0.0013 0.0006 0.0007 0.0012 0.0023 0.0026 0.0056 0.0022 0.0003 0.0019
Vacuole Periphery 0.0115 0.0001 0 0.0002 0.0001 0.0004 0.0007 0.001 0.0004 0.0003 0.0005 0.0005 0.0007 0.0003 0.0023 0.0002 0.0001 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.7152 14.3473 39.9237 153.9837 119.6512 15.3723 15.111 16.9301 85.2862 155.6867
Translational Efficiency 0.1324 0.1742 0.2881 0.2099 0.3775 0.1662 0.1473 0.1551 0.2286 0.3435

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Modulator of cell separation and mitotic exit; inhibits separation through Ub-dependent Ace2p proteolysis; part of a daughter-specific switch induced by the mitotic exit network that inhibits exit and resets the cell cycle after the execution of MEN function, blocking Tem1p and Cdc15 association; required for chromosome stability and multiple mitotic checkpoints; regulated by SCF; haploid transcription regulated by Ste12p; contains 12 degenerate leucine-rich repeat motifs and an atypical F-box
Localization
Cell Percentages cytoplasm (26%), nucleus (7%), mixed (39%)
Cell Cycle Regulation Yes
cytoplasm - S/G2 (2.6e-11)
cytoplasm - MA (8.8e-12)
nucleus - T/G1 (1.6e-14)
Subcompartmental Group N/A

Amn1

Amn1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Amn1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available