Standard name
Description Modulator of cell separation and mitotic exit; inhibits separation through Ub-dependent Ace2p proteolysis; part of a daughter-specific switch induced by the mitotic exit network that inhibits exit and resets the cell cycle after the execution of MEN function, blocking Tem1p and Cdc15 association; required for chromosome stability and multiple mitotic checkpoints; regulated by SCF; haploid transcription regulated by Ste12p; contains 12 degenerate leucine-rich repeat motifs and an atypical F-box

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.67 0.81 0.94 0.97 0.88 0.73 0.68 0.67 0.58 0.57 0.38 0.28 0.97 0.99 0.99 0.95 0.95 0.46 0.56 0.56 0.8 0.73 0.65
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.08 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.16 0 0 0.13 0.14 0.45 0.46 0.4 0.64 0.65 0.75 0.84 0 0 0 0 0 0.07 0.06 0.2 0.05 0 0.06
Nucleus 0.11 0.09 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.16 0.11 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.13 0.07 0 0 0 0
Vac/Vac Membrane 0.13 0.13 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0.07 0.09 0.05 0 0.05 0.09
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 5 12 0 3 8
Bud 3 1 3 0 2 10 7 9 10 11 6 4 0 2 0 0 0 6 20 8 2 5 10
Bud Neck 0 1 1 1 3 3 4 8 1 3 0 2 0 0 0 2 3 0 2 6 0 1 4
Bud Site 0 0 0 0 0 2 0 0 5 2 2 2 0 0 0 0 0
Cell Periphery 2 1 1 2 1 0 0 2 0 0 0 2 1 0 1 11 6 0 2 2 1 1 3
Cytoplasm 102 366 395 222 263 310 351 298 240 279 78 74 266 338 364 525 507 84 260 251 136 221 217
Endoplasmic Reticulum 0 0 1 0 2 0 0 1 0 0 5 6 2 0 4 49 42 1 1 4 1 3 5
Endosome 3 3 1 0 3 2 0 0 1 1 0 0 0 3 0 6 5 1 4 14 0 3 4
Golgi 0 0 1 0 1 1 0 0 0 0 0 0 0 0 0 1 0 0 1 1 1 3 0
Mitochondria 24 11 13 29 43 190 235 179 265 317 152 221 0 2 0 10 10 13 28 89 8 13 21
Nucleus 17 39 3 0 2 2 3 2 3 2 1 1 4 0 0 1 2 29 51 5 2 10 6
Nuclear Periphery 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 1 0 0 0 1 1 0 1 0 2 1 1 1 0 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 2 0 1 1 0 0 0
SpindlePole 1 0 2 0 1 5 8 13 7 8 2 5 0 0 0 4 1 22 32 12 4 12 10
Vac/Vac Membrane 20 60 19 1 3 5 8 6 5 5 1 3 5 0 1 35 22 13 43 20 6 14 28
Unique Cell Count 153 450 419 230 298 426 514 446 417 491 204 264 274 342 369 553 534 182 465 446 171 304 333
Labelled Cell Count 173 482 440 255 326 531 616 519 537 631 248 322 279 345 370 645 601 182 465 446 171 304 333


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.9 4.3 4.0 3.5 3.8 3.2 2.6 3.0 2.9 2.5 2.7 2.5 4.5 4.2 4.4 4.8 5.9 5.4 4.3 4.5 5.0
Std Deviation (1e-4) 1.4 0.8 1.6 1.5 1.8 1.4 0.7 1.0 1.1 0.7 0.6 0.7 1.4 1.2 1.6 1.9 1.9 2.0 1.9 1.9 1.5
Intensity Change (Log2) -0.18 -0.07 -0.31 -0.63 -0.43 -0.47 -0.66 -0.53 -0.65 0.16 0.07 0.14 0.28 0.57 0.45 0.1 0.17 0.32


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0
Bud Neck 0 0
Bud Site 0 0
Cell Periphery 0 0
Cyto
Endoplasmic Reticulum 0 0
Endosome 0 0
Golgi 0 0
Mitochondria 0 0
Nuclear Periphery 0 0
Nuc
Nucleolus 0 0
Peroxisomes 0 0
SpindlePole 0 0
Vac
Cortical Patches 0 0
Cytoplasm 0 0
Nucleus 0 0
Vacuole 1.2 -0.3


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Amn1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available