Standard name | |
---|---|
Human Ortholog | |
Description | Cyclin-dependent kinase (CDK) catalytic subunit; master regulator of mitotic and meiotic cell cycles; alternately associates with G1, S, G2/M phase cyclins, which provide substrate specificity; regulates metabolism, basal transcription, chromosome dynamics, growth and morphogenesis; transcript induction in osmostress involves antisense RNA; human homologs CDK1, CDK2, CDK3 can complement yeast conditional cdc28 mutants; human CDK1, CDK2 can complement yeast cdc28 null mutant |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 2 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 1 | 1 | 2 | 0 | 0 | 2 | 3 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 0 |
Bud | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 1 | 1 | 4 | 0 | 0 | 0 | 0 | 1 | 0 | 33 | 43 | 9 | 9 | 3 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 2 | 0 | 0 | 0 | 0 | 2 | 2 | 2 | 2 | 6 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 157 | 202 | 104 | 62 | 91 | 148 | 197 | 172 | 158 | 154 | 144 | 150 | 40 | 81 | 97 | 70 | 72 | 64 | 118 | 155 | 76 | 111 | 172 | 161 |
Endoplasmic Reticulum | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 2 | 4 | 1 | 0 | 0 | 0 | 1 | 8 | 3 | 0 | 1 | 1 | 0 | 0 | 2 |
Endosome | 1 | 1 | 0 | 1 | 2 | 3 | 3 | 3 | 1 | 2 | 2 | 2 | 1 | 6 | 3 | 1 | 0 | 2 | 5 | 7 | 4 | 1 | 2 | 2 |
Golgi | 4 | 0 | 6 | 2 | 12 | 5 | 11 | 6 | 0 | 0 | 0 | 3 | 0 | 1 | 1 | 1 | 7 | 7 | 0 | 0 | 1 | 0 | 0 | 0 |
Mitochondria | 1 | 2 | 0 | 0 | 0 | 4 | 3 | 5 | 42 | 22 | 23 | 45 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 1 | 3 | 0 | 2 | 2 |
Nucleus | 34 | 74 | 35 | 27 | 59 | 51 | 73 | 66 | 49 | 60 | 42 | 57 | 22 | 43 | 75 | 24 | 20 | 20 | 112 | 192 | 66 | 33 | 27 | 23 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 3 | 2 | 0 | 1 | 0 |
Nucleolus | 12 | 7 | 3 | 1 | 8 | 5 | 9 | 22 | 13 | 17 | 13 | 14 | 7 | 5 | 11 | 3 | 2 | 2 | 14 | 9 | 4 | 1 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 3 | 4 | 0 | 0 | 0 | 14 | 15 | 7 | 1 | 0 | 0 |
Vac/Vac Membrane | 107 | 148 | 64 | 46 | 88 | 77 | 121 | 167 | 54 | 81 | 62 | 81 | 35 | 62 | 61 | 44 | 62 | 67 | 14 | 28 | 15 | 15 | 15 | 9 |
Unique Cell Count | 283 | 410 | 185 | 129 | 245 | 278 | 392 | 416 | 295 | 327 | 272 | 328 | 95 | 177 | 220 | 130 | 144 | 144 | 322 | 467 | 198 | 180 | 229 | 209 |
Labelled Cell Count | 319 | 437 | 213 | 139 | 263 | 293 | 420 | 445 | 322 | 343 | 300 | 359 | 105 | 204 | 256 | 149 | 173 | 165 | 322 | 467 | 198 | 180 | 229 | 209 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 16.9 | 11.3 | 14.9 | 14.4 | 15.3 | 13.2 | 13.5 | 12.8 | 11.3 | 11.9 | 11.7 | 11.6 | 13.7 | 14.8 | 15.6 | 13.9 | 18.0 | 18.3 | 14.3 | 14.7 | 13.8 |
Std Deviation (1e-4) | 2.9 | 2.2 | 2.9 | 3.4 | 3.7 | 2.9 | 3.7 | 2.9 | 3.0 | 3.0 | 2.7 | 2.7 | 4.0 | 4.4 | 5.4 | 3.7 | 4.7 | 4.9 | 2.9 | 2.9 | 3.1 |
Intensity Change (Log2) | – | – | – | -0.05 | 0.04 | -0.17 | -0.15 | -0.22 | -0.4 | -0.33 | -0.35 | -0.36 | -0.12 | -0.01 | 0.07 | -0.1 | 0.27 | 0.3 | -0.06 | -0.02 | -0.11 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -1.4 | -3.9 | -0.6 | -1.3 | -3.4 | -0.6 | -2.0 | -0.7 | -2.3 | -2.2 | -2.0 | -2.4 | -0.4 | -1.1 | -2.1 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0.8 | 0 | -0.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.7 | 0.7 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 5.4 | 3.6 | 4.1 | 5.3 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0.4 | 1.3 | -0.2 | -0.1 | -0.9 | -0.6 | -0.2 | -1.0 | -0.4 | 0.8 | 1.2 | 3.4 | -0.1 | -1.2 | -1.2 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 2.1 | 1.7 | 2.0 | 1.8 | 1.6 | 0 | 0 | 1.9 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0.2 | 0.3 | -1.6 | -0.9 | 1.3 | -4.0 | -2.4 | -2.8 | -2.4 | 0.4 | 0.1 | -1.5 | -0.1 | 1.6 | 2.2 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 11.9538 | 17.071 | 15.2696 | 15.2944 | 14.397 | 14.2924 | 11.3257 | 14.7297 | 14.296 | 14.5666 | 12.0788 | 13.548 | 8.556 | 12.8147 | 11.741 | 10.7722 | 10.5099 | 10.67 |
Actin | 0.0414 | 0.0019 | 0.0091 | 0.0004 | 0.0032 | 0.0083 | 0.0241 | 0.0005 | 0.0059 | 0.0002 | 0.0023 | 0.002 | 0.0284 | 0.0007 | 0.0034 | 0.0018 | 0.0055 | 0.0016 |
Bud | 0.0016 | 0.0007 | 0.0007 | 0.0001 | 0.0005 | 0.0006 | 0.005 | 0.0006 | 0.0002 | 0 | 0.0001 | 0.0003 | 0.003 | 0.0007 | 0.0005 | 0.0001 | 0.0004 | 0.0003 |
Bud Neck | 0.0054 | 0.0009 | 0.0014 | 0.0015 | 0.0046 | 0.007 | 0.0033 | 0.0006 | 0.0008 | 0.0018 | 0.0026 | 0.0038 | 0.0029 | 0.0004 | 0.0008 | 0.0018 | 0.0029 | 0.0051 |
Bud Periphery | 0.0012 | 0.0002 | 0.0009 | 0.0001 | 0.001 | 0.0003 | 0.0026 | 0.0002 | 0.0002 | 0 | 0.0002 | 0.0001 | 0.0016 | 0.0002 | 0.0003 | 0.0003 | 0.0004 | 0.0002 |
Bud Site | 0.0042 | 0.0018 | 0.004 | 0.0001 | 0.0025 | 0.0005 | 0.0038 | 0.0011 | 0.0004 | 0.0001 | 0.0004 | 0.0002 | 0.0035 | 0.001 | 0.0011 | 0.0002 | 0.0006 | 0.0004 |
Cell Periphery | 0.0009 | 0.0001 | 0.0002 | 0 | 0.0005 | 0.0001 | 0.0003 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 |
Cytoplasm | 0.3888 | 0.2056 | 0.0092 | 0.0134 | 0.0133 | 0.2591 | 0.3623 | 0.216 | 0.032 | 0.0047 | 0.0107 | 0.2715 | 0.4548 | 0.1615 | 0.0195 | 0.0064 | 0.0152 | 0.2934 |
Cytoplasmic Foci | 0.0136 | 0.0004 | 0.0067 | 0.0001 | 0.0027 | 0.0073 | 0.0189 | 0.0014 | 0.0059 | 0 | 0.0363 | 0.0041 | 0.0092 | 0.01 | 0.004 | 0.0018 | 0.0018 | 0.0015 |
Eisosomes | 0.0009 | 0 | 0.0002 | 0 | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0002 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0079 | 0.0011 | 0.0008 | 0.0006 | 0.0043 | 0.0024 | 0.0152 | 0.0008 | 0.001 | 0.0001 | 0.0014 | 0.0018 | 0.0101 | 0.0007 | 0.0013 | 0.002 | 0.0024 | 0.0019 |
Endosome | 0.0087 | 0.0003 | 0.0052 | 0.0004 | 0.0062 | 0.0053 | 0.0106 | 0.0004 | 0.0037 | 0.0001 | 0.0284 | 0.0022 | 0.0137 | 0.0005 | 0.0042 | 0.0025 | 0.0058 | 0.0015 |
Golgi | 0.0048 | 0 | 0.0016 | 0 | 0.0009 | 0.0036 | 0.0016 | 0 | 0.0012 | 0 | 0.0077 | 0.0003 | 0.0072 | 0.0001 | 0.0011 | 0.0009 | 0.0006 | 0.0011 |
Lipid Particles | 0.0059 | 0 | 0.0129 | 0 | 0.0106 | 0.0049 | 0.0048 | 0 | 0.0036 | 0 | 0.0219 | 0.0002 | 0.0009 | 0.0001 | 0.0009 | 0.0014 | 0.0008 | 0.0013 |
Mitochondria | 0.0029 | 0.0003 | 0.0016 | 0.0004 | 0.0249 | 0.0024 | 0.0019 | 0.0004 | 0.0105 | 0.0002 | 0.006 | 0.0009 | 0.0035 | 0.0004 | 0.0012 | 0.0306 | 0.0006 | 0.002 |
None | 0.0105 | 0.0005 | 0.0012 | 0.0002 | 0.0017 | 0.0079 | 0.0032 | 0.0005 | 0.0004 | 0 | 0.0003 | 0.0068 | 0.0032 | 0.0003 | 0.0006 | 0.0012 | 0.0023 | 0.0005 |
Nuclear Periphery | 0.0418 | 0.0173 | 0.0124 | 0.0302 | 0.0568 | 0.0201 | 0.0621 | 0.0118 | 0.0198 | 0.0237 | 0.0313 | 0.0163 | 0.0455 | 0.0093 | 0.0153 | 0.0201 | 0.022 | 0.0155 |
Nucleolus | 0.0038 | 0.0022 | 0.0049 | 0.0035 | 0.0154 | 0.0028 | 0.0032 | 0.0021 | 0.0038 | 0.0037 | 0.0128 | 0.0019 | 0.0015 | 0.0021 | 0.004 | 0.003 | 0.0075 | 0.0017 |
Nucleus | 0.424 | 0.7579 | 0.894 | 0.9447 | 0.8299 | 0.6502 | 0.4211 | 0.7591 | 0.8755 | 0.9636 | 0.7984 | 0.6659 | 0.3919 | 0.808 | 0.9341 | 0.9141 | 0.9083 | 0.6611 |
Peroxisomes | 0.0083 | 0.0001 | 0.0093 | 0 | 0.0018 | 0.0088 | 0.0067 | 0.0001 | 0.0034 | 0 | 0.0044 | 0.0013 | 0.0011 | 0.0004 | 0.0008 | 0.002 | 0.0003 | 0.0009 |
Punctate Nuclear | 0.0145 | 0.008 | 0.0215 | 0.0036 | 0.0133 | 0.0063 | 0.0384 | 0.0036 | 0.0305 | 0.0014 | 0.0328 | 0.0193 | 0.0102 | 0.0024 | 0.0058 | 0.009 | 0.0212 | 0.0084 |
Vacuole | 0.0077 | 0.0006 | 0.0019 | 0.0005 | 0.0042 | 0.0018 | 0.009 | 0.0007 | 0.0005 | 0.0002 | 0.0011 | 0.0009 | 0.0059 | 0.0011 | 0.0009 | 0.0003 | 0.0008 | 0.0012 |
Vacuole Periphery | 0.001 | 0.0001 | 0.0004 | 0.0003 | 0.0016 | 0.0004 | 0.0017 | 0.0002 | 0.0004 | 0.0001 | 0.0007 | 0.0002 | 0.0014 | 0.0002 | 0.0003 | 0.0004 | 0.0004 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 63.9655 | 27.491 | 27.7433 | 41.8848 | 41.467 | 39.3416 | 32.7994 | 30.392 | 32.708 | 44.7326 |
Translational Efficiency | 1.7462 | 2.1214 | 1.6036 | 1.5202 | 1.8088 | 3.0111 | 1.8919 | 1.4326 | 1.7611 | 1.5961 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✔ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2871 | 1683 | 398 | 189 | 2056 | 2327 | 331 | 1632 | 4927 | 4010 | 729 | 1821 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1370.48 | 1462.32 | 1729.82 | 1925.39 | 1252.40 | 1448.32 | 1849.31 | 2075.36 | 1321.21 | 1454.20 | 1784.07 | 2059.79 |
Standard Deviation | 201.15 | 240.23 | 224.76 | 362.23 | 187.85 | 222.98 | 268.84 | 359.77 | 204.19 | 230.48 | 252.85 | 362.92 |
Intensity Change Log 2 | — | 0.093578 | 0.335941 | 0.490469 | — | 0.209685 | 0.562292 | 0.728666 | — | 0.150185 | 0.448457 | 0.609119 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000424 | 0.000902 | 0.001368 | 0.001062 | 0.000656 | 0.000848 | 0.001932 | 0.000592 | 0.000521 | 0.000871 | 0.001624 | 0.000641 |
Bud Neck | 0.035822 | 0.026146 | 0.024783 | 0.018597 | 0.023221 | 0.026669 | 0.020157 | 0.024409 | 0.030563 | 0.026450 | 0.022683 | 0.023805 |
Bud Site | 0.008330 | 0.011420 | 0.011275 | 0.013829 | 0.011111 | 0.012336 | 0.005809 | 0.010107 | 0.009491 | 0.011951 | 0.008793 | 0.010494 |
Cell Periphery | 0.000259 | 0.000369 | 0.000437 | 0.000451 | 0.000276 | 0.000266 | 0.000387 | 0.000315 | 0.000266 | 0.000309 | 0.000414 | 0.000329 |
Cytoplasm | 0.302013* | 0.302497* | 0.074278 | 0.106668* | 0.389352* | 0.310497* | 0.035619 | 0.078617 | 0.338459* | 0.307139* | 0.056725 | 0.081528 |
Cytoplasmic Foci | 0.003174 | 0.005818 | 0.001533 | 0.026291 | 0.005600 | 0.005182 | 0.001103 | 0.008394 | 0.004187 | 0.005449 | 0.001338 | 0.010252 |
Eisosomes | 0.000016 | 0.000016 | 0.000037 | 0.000050 | 0.000013 | 0.000015 | 0.000059 | 0.000015 | 0.000015 | 0.000016 | 0.000047 | 0.000019 |
Endoplasmic Reticulum | 0.002620 | 0.002239 | 0.002712 | 0.001141 | 0.002642 | 0.002288 | 0.002498 | 0.000925 | 0.002629 | 0.002267 | 0.002615 | 0.000947 |
Endosome | 0.000740 | 0.001064 | 0.000706 | 0.013037 | 0.000878 | 0.001080 | 0.000267 | 0.000325 | 0.000797 | 0.001073 | 0.000507 | 0.001644 |
Golgi | 0.000125 | 0.000795 | 0.000253 | 0.011124 | 0.000286 | 0.000734 | 0.000172 | 0.000163 | 0.000192 | 0.000760 | 0.000216 | 0.001300 |
Lipid Particles | 0.000267 | 0.000432 | 0.000545 | 0.040802 | 0.000336 | 0.000351 | 0.000428 | 0.000397 | 0.000296 | 0.000385 | 0.000492 | 0.004590 |
Mitochondria | 0.001181 | 0.001525 | 0.002593 | 0.006729 | 0.001093 | 0.001632 | 0.002582 | 0.002450 | 0.001145 | 0.001587 | 0.002588 | 0.002894 |
Mitotic Spindle | 0.008050 | 0.017138 | 0.004153 | 0.198275* | 0.012571 | 0.016106 | 0.003284 | 0.078586 | 0.009936 | 0.016539 | 0.003758 | 0.091009 |
None | 0.011804 | 0.013893 | 0.005085 | 0.042024 | 0.013418 | 0.015966 | 0.004530 | 0.009394 | 0.012477 | 0.015096 | 0.004833 | 0.012781 |
Nuclear Periphery | 0.001525 | 0.000859 | 0.001658 | 0.001333 | 0.000960 | 0.001011 | 0.000976 | 0.001782 | 0.001289 | 0.000948 | 0.001349 | 0.001735 |
Nuclear Periphery Foci | 0.001494 | 0.002745 | 0.001095 | 0.002227 | 0.002892 | 0.001826 | 0.000439 | 0.000691 | 0.002078 | 0.002212 | 0.000798 | 0.000851 |
Nucleolus | 0.007793 | 0.005754 | 0.002376 | 0.001494 | 0.004908 | 0.006254 | 0.001977 | 0.001779 | 0.006589 | 0.006044 | 0.002195 | 0.001749 |
Nucleus | 0.608689* | 0.598772* | 0.848274* | 0.489282* | 0.522930* | 0.588889* | 0.906972* | 0.773407* | 0.572903* | 0.593037* | 0.874925* | 0.743918* |
Peroxisomes | 0.000303 | 0.000436 | 0.000331 | 0.003149 | 0.000342 | 0.000571 | 0.000532 | 0.000754 | 0.000319 | 0.000515 | 0.000423 | 0.001002 |
Vacuole | 0.005200 | 0.006952 | 0.016308 | 0.021705 | 0.006189 | 0.006988 | 0.010156 | 0.006762 | 0.005612 | 0.006973 | 0.013515 | 0.008313 |
Vacuole Periphery | 0.000170 | 0.000228 | 0.000199 | 0.000730 | 0.000325 | 0.000491 | 0.000122 | 0.000136 | 0.000235 | 0.000381 | 0.000164 | 0.000198 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -6.42 | -8.17 | -4.58 | -2.66 | 0.01 | -3.20 | -6.68 | -6.12 | -1.63 | 4.54 | -6.47 | -10.37 | -8.91 | -2.45 | 5.31 |
Bud Neck | -0.99 | -0.44 | 0.46 | 0.94 | 0.67 | -2.60 | -2.90 | -5.77 | -3.49 | -0.27 | -1.95 | -2.22 | -4.41 | -2.78 | -0.76 |
Bud Site | -5.59 | -2.52 | -4.04 | -2.41 | -2.51 | -2.46 | -0.34 | -3.76 | -1.54 | -2.21 | -6.06 | -2.27 | -6.85 | -2.38 | -2.78 |
Cell Periphery | -7.19 | -8.96 | -6.30 | -4.60 | -2.28 | 0.34 | -7.09 | -10.26 | -10.80 | 1.07 | -5.07 | -11.44 | -13.36 | -10.24 | 0.58 |
Cytoplasm | 1.72 | 28.06 | 16.61 | 14.46 | -1.95 | 9.82 | 40.76 | 38.61 | 31.36 | -8.41 | 7.18 | 47.29 | 47.55 | 38.48 | -5.05 |
Cytoplasmic Foci | -4.12 | 2.29 | -2.11 | -1.23 | -2.39 | 0.77 | 5.26 | -0.38 | -1.00 | -4.39 | -2.82 | 5.38 | -2.73 | -1.04 | -5.25 |
Eisosomes | -1.49 | -6.86 | -5.99 | -5.67 | -1.09 | -2.92 | -7.97 | -10.43 | -8.39 | 5.59 | -2.73 | -10.45 | -11.71 | -9.92 | 5.89 |
Endoplasmic Reticulum | 2.16 | -1.70 | 2.26 | 0.66 | 3.07 | 0.31 | -1.45 | 6.29 | 6.26 | 5.30 | 1.88 | -2.18 | 9.51 | 6.93 | 7.56 |
Endosome | -0.72 | -0.49 | -1.27 | -1.06 | -1.18 | -0.68 | 3.10 | 1.86 | 2.75 | -2.34 | -1.06 | 1.96 | 1.23 | 1.91 | -0.42 |
Golgi | -2.51 | -5.48 | -1.62 | -0.78 | -1.32 | -2.50 | -0.89 | -0.58 | 2.42 | 0.58 | -3.76 | -4.00 | -2.67 | 1.86 | -0.80 |
Lipid Particles | -3.23 | -6.38 | -2.27 | -2.17 | -1.96 | -1.38 | -3.99 | -9.47 | -9.00 | -4.87 | -3.36 | -7.30 | -7.12 | -6.56 | -4.62 |
Mitochondria | -4.21 | -10.30 | -4.70 | -3.45 | 0.39 | -2.84 | -11.06 | -22.93 | -12.78 | -2.96 | -4.14 | -15.05 | -24.21 | -15.72 | -3.85 |
Mitotic Spindle | -3.28 | -1.35 | -5.01 | -4.36 | -4.40 | -1.57 | 2.03 | -12.39 | -11.57 | -12.84 | -3.66 | -0.16 | -14.17 | -12.49 | -12.52 |
None | -2.70 | 13.12 | -0.75 | -0.15 | -3.29 | -3.62 | 12.09 | -0.69 | 2.84 | -12.33 | -5.21 | 17.84 | -2.21 | 1.65 | -13.97 |
Nuclear Periphery | 4.50 | -4.86 | -6.81 | -8.52 | -4.01 | -0.01 | -4.99 | -17.64 | -16.57 | -11.78 | 3.32 | -6.61 | -17.34 | -19.14 | -11.34 |
Nuclear Periphery Foci | -1.40 | 1.02 | 1.63 | 2.92 | -0.17 | 1.63 | 3.96 | 4.29 | 3.56 | -0.70 | 0.31 | 2.85 | 5.36 | 5.54 | 0.12 |
Nucleolus | 1.68 | 7.34 | 8.48 | 6.68 | 1.03 | -3.96 | 2.38 | 2.71 | 6.62 | -0.25 | -0.99 | 7.39 | 7.23 | 8.07 | -0.76 |
Nucleus | 1.83 | -12.29 | 1.93 | 1.28 | 7.65 | -6.39 | -21.33 | -13.95 | -8.57 | 10.59 | -2.21 | -22.85 | -9.88 | -8.08 | 11.84 |
Peroxisomes | -2.17 | -1.60 | -1.70 | -0.91 | -1.11 | -2.92 | -2.10 | -0.64 | 1.66 | 1.73 | -3.65 | -2.58 | -0.96 | 1.38 | 1.51 |
Vacuole | -2.92 | -6.44 | -8.05 | -7.85 | -5.53 | -2.05 | -4.39 | -13.14 | -12.08 | -3.80 | -3.57 | -7.78 | -15.37 | -14.26 | -4.15 |
Vacuole Periphery | -2.37 | -3.75 | -2.08 | -0.95 | -0.36 | -0.85 | 1.43 | 2.00 | 1.92 | 1.68 | -1.47 | -0.56 | 1.35 | 2.09 | 3.35 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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