Standard name
Human Ortholog
Description Cyclin-dependent kinase (CDK) catalytic subunit; master regulator of mitotic and meiotic cell cycles; alternately associates with G1, S, G2/M phase cyclins, which provide substrate specificity; regulates metabolism, basal transcription, chromosome dynamics, growth and morphogenesis; transcript induction in osmostress involves antisense RNA; human homologs CDK1, CDK2, CDK3 can complement yeast conditional cdc28 mutants; human CDK1, CDK2 can complement yeast cdc28 null mutant

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0.09 0.05 0.05 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.55 0.49 0.56 0.48 0.37 0.53 0.5 0.41 0.54 0.47 0.53 0.46 0.42 0.46 0.44 0.54 0.5 0.44 0.37 0.33 0.39 0.62 0.75 0.77
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.14 0.07 0.08 0.14 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.12 0.18 0.19 0.21 0.24 0.18 0.19 0.16 0.17 0.18 0.15 0.17 0.23 0.24 0.34 0.18 0.14 0.14 0.35 0.41 0.34 0.19 0.12 0.11
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0.05 0 0.05 0.05 0 0.07 0 0.05 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.38 0.36 0.35 0.36 0.36 0.28 0.31 0.4 0.18 0.25 0.23 0.25 0.37 0.35 0.28 0.34 0.43 0.47 0 0.06 0.08 0.08 0.07 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 0 1 0 1 0 1 0 1 1 2 0 0 2 3 1 1 0 1 1 0 1 0 0
Bud 0 1 0 0 1 0 0 2 0 1 1 4 0 0 0 0 1 0 33 43 9 9 3 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Cell Periphery 0 2 0 0 0 0 2 2 2 2 6 1 0 0 0 1 0 0 0 0 0 0 0 0
Cytoplasm 157 202 104 62 91 148 197 172 158 154 144 150 40 81 97 70 72 64 118 155 76 111 172 161
Endoplasmic Reticulum 1 0 0 0 1 0 0 0 2 2 4 1 0 0 0 1 8 3 0 1 1 0 0 2
Endosome 1 1 0 1 2 3 3 3 1 2 2 2 1 6 3 1 0 2 5 7 4 1 2 2
Golgi 4 0 6 2 12 5 11 6 0 0 0 3 0 1 1 1 7 7 0 0 1 0 0 0
Mitochondria 1 2 0 0 0 4 3 5 42 22 23 45 0 0 0 3 0 0 0 1 3 0 2 2
Nucleus 34 74 35 27 59 51 73 66 49 60 42 57 22 43 75 24 20 20 112 192 66 33 27 23
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 3 2 0 1 0
Nucleolus 12 7 3 1 8 5 9 22 13 17 13 14 7 5 11 3 2 2 14 9 4 1 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 1 0 1 0 3 4 0 0 0 14 15 7 1 0 0
Vac/Vac Membrane 107 148 64 46 88 77 121 167 54 81 62 81 35 62 61 44 62 67 14 28 15 15 15 9
Unique Cell Count 283 410 185 129 245 278 392 416 295 327 272 328 95 177 220 130 144 144 322 467 198 180 229 209
Labelled Cell Count 319 437 213 139 263 293 420 445 322 343 300 359 105 204 256 149 173 165 322 467 198 180 229 209


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 16.9 11.3 14.9 14.4 15.3 13.2 13.5 12.8 11.3 11.9 11.7 11.6 13.7 14.8 15.6 13.9 18.0 18.3 14.3 14.7 13.8
Std Deviation (1e-4) 2.9 2.2 2.9 3.4 3.7 2.9 3.7 2.9 3.0 3.0 2.7 2.7 4.0 4.4 5.4 3.7 4.7 4.9 2.9 2.9 3.1
Intensity Change (Log2) -0.05 0.04 -0.17 -0.15 -0.22 -0.4 -0.33 -0.35 -0.36 -0.12 -0.01 0.07 -0.1 0.27 0.3 -0.06 -0.02 -0.11


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.4 -3.9 -0.6 -1.3 -3.4 -0.6 -2.0 -0.7 -2.3 -2.2 -2.0 -2.4 -0.4 -1.1 -2.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0.8 0 -0.3 0 0 0 0 0 0 0 0 0 0.7 0.7
Mitochondria 0 0 0 0 0 5.4 3.6 4.1 5.3 0 0 0 0 0 0
Nucleus 0.4 1.3 -0.2 -0.1 -0.9 -0.6 -0.2 -1.0 -0.4 0.8 1.2 3.4 -0.1 -1.2 -1.2
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 2.1 1.7 2.0 1.8 1.6 0 0 1.9 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0.2 0.3 -1.6 -0.9 1.3 -4.0 -2.4 -2.8 -2.4 0.4 0.1 -1.5 -0.1 1.6 2.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 11.9538 17.071 15.2696 15.2944 14.397 14.2924 11.3257 14.7297 14.296 14.5666 12.0788 13.548 8.556 12.8147 11.741 10.7722 10.5099 10.67
Actin 0.0414 0.0019 0.0091 0.0004 0.0032 0.0083 0.0241 0.0005 0.0059 0.0002 0.0023 0.002 0.0284 0.0007 0.0034 0.0018 0.0055 0.0016
Bud 0.0016 0.0007 0.0007 0.0001 0.0005 0.0006 0.005 0.0006 0.0002 0 0.0001 0.0003 0.003 0.0007 0.0005 0.0001 0.0004 0.0003
Bud Neck 0.0054 0.0009 0.0014 0.0015 0.0046 0.007 0.0033 0.0006 0.0008 0.0018 0.0026 0.0038 0.0029 0.0004 0.0008 0.0018 0.0029 0.0051
Bud Periphery 0.0012 0.0002 0.0009 0.0001 0.001 0.0003 0.0026 0.0002 0.0002 0 0.0002 0.0001 0.0016 0.0002 0.0003 0.0003 0.0004 0.0002
Bud Site 0.0042 0.0018 0.004 0.0001 0.0025 0.0005 0.0038 0.0011 0.0004 0.0001 0.0004 0.0002 0.0035 0.001 0.0011 0.0002 0.0006 0.0004
Cell Periphery 0.0009 0.0001 0.0002 0 0.0005 0.0001 0.0003 0 0.0001 0 0.0001 0 0.0003 0.0001 0.0001 0 0.0001 0.0001
Cytoplasm 0.3888 0.2056 0.0092 0.0134 0.0133 0.2591 0.3623 0.216 0.032 0.0047 0.0107 0.2715 0.4548 0.1615 0.0195 0.0064 0.0152 0.2934
Cytoplasmic Foci 0.0136 0.0004 0.0067 0.0001 0.0027 0.0073 0.0189 0.0014 0.0059 0 0.0363 0.0041 0.0092 0.01 0.004 0.0018 0.0018 0.0015
Eisosomes 0.0009 0 0.0002 0 0.0003 0.0001 0.0001 0 0.0002 0 0 0 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.0079 0.0011 0.0008 0.0006 0.0043 0.0024 0.0152 0.0008 0.001 0.0001 0.0014 0.0018 0.0101 0.0007 0.0013 0.002 0.0024 0.0019
Endosome 0.0087 0.0003 0.0052 0.0004 0.0062 0.0053 0.0106 0.0004 0.0037 0.0001 0.0284 0.0022 0.0137 0.0005 0.0042 0.0025 0.0058 0.0015
Golgi 0.0048 0 0.0016 0 0.0009 0.0036 0.0016 0 0.0012 0 0.0077 0.0003 0.0072 0.0001 0.0011 0.0009 0.0006 0.0011
Lipid Particles 0.0059 0 0.0129 0 0.0106 0.0049 0.0048 0 0.0036 0 0.0219 0.0002 0.0009 0.0001 0.0009 0.0014 0.0008 0.0013
Mitochondria 0.0029 0.0003 0.0016 0.0004 0.0249 0.0024 0.0019 0.0004 0.0105 0.0002 0.006 0.0009 0.0035 0.0004 0.0012 0.0306 0.0006 0.002
None 0.0105 0.0005 0.0012 0.0002 0.0017 0.0079 0.0032 0.0005 0.0004 0 0.0003 0.0068 0.0032 0.0003 0.0006 0.0012 0.0023 0.0005
Nuclear Periphery 0.0418 0.0173 0.0124 0.0302 0.0568 0.0201 0.0621 0.0118 0.0198 0.0237 0.0313 0.0163 0.0455 0.0093 0.0153 0.0201 0.022 0.0155
Nucleolus 0.0038 0.0022 0.0049 0.0035 0.0154 0.0028 0.0032 0.0021 0.0038 0.0037 0.0128 0.0019 0.0015 0.0021 0.004 0.003 0.0075 0.0017
Nucleus 0.424 0.7579 0.894 0.9447 0.8299 0.6502 0.4211 0.7591 0.8755 0.9636 0.7984 0.6659 0.3919 0.808 0.9341 0.9141 0.9083 0.6611
Peroxisomes 0.0083 0.0001 0.0093 0 0.0018 0.0088 0.0067 0.0001 0.0034 0 0.0044 0.0013 0.0011 0.0004 0.0008 0.002 0.0003 0.0009
Punctate Nuclear 0.0145 0.008 0.0215 0.0036 0.0133 0.0063 0.0384 0.0036 0.0305 0.0014 0.0328 0.0193 0.0102 0.0024 0.0058 0.009 0.0212 0.0084
Vacuole 0.0077 0.0006 0.0019 0.0005 0.0042 0.0018 0.009 0.0007 0.0005 0.0002 0.0011 0.0009 0.0059 0.0011 0.0009 0.0003 0.0008 0.0012
Vacuole Periphery 0.001 0.0001 0.0004 0.0003 0.0016 0.0004 0.0017 0.0002 0.0004 0.0001 0.0007 0.0002 0.0014 0.0002 0.0003 0.0004 0.0004 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 63.9655 27.491 27.7433 41.8848 41.467 39.3416 32.7994 30.392 32.708 44.7326
Translational Efficiency 1.7462 2.1214 1.6036 1.5202 1.8088 3.0111 1.8919 1.4326 1.7611 1.5961

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2871 1683 398 189 2056 2327 331 1632 4927 4010 729 1821

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1370.48 1462.32 1729.82 1925.39 1252.40 1448.32 1849.31 2075.36 1321.21 1454.20 1784.07 2059.79
Standard Deviation 201.15 240.23 224.76 362.23 187.85 222.98 268.84 359.77 204.19 230.48 252.85 362.92
Intensity Change Log 2 0.093578 0.335941 0.490469 0.209685 0.562292 0.728666 0.150185 0.448457 0.609119

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000424 0.000902 0.001368 0.001062 0.000656 0.000848 0.001932 0.000592 0.000521 0.000871 0.001624 0.000641
Bud Neck 0.035822 0.026146 0.024783 0.018597 0.023221 0.026669 0.020157 0.024409 0.030563 0.026450 0.022683 0.023805
Bud Site 0.008330 0.011420 0.011275 0.013829 0.011111 0.012336 0.005809 0.010107 0.009491 0.011951 0.008793 0.010494
Cell Periphery 0.000259 0.000369 0.000437 0.000451 0.000276 0.000266 0.000387 0.000315 0.000266 0.000309 0.000414 0.000329
Cytoplasm 0.302013* 0.302497* 0.074278 0.106668* 0.389352* 0.310497* 0.035619 0.078617 0.338459* 0.307139* 0.056725 0.081528
Cytoplasmic Foci 0.003174 0.005818 0.001533 0.026291 0.005600 0.005182 0.001103 0.008394 0.004187 0.005449 0.001338 0.010252
Eisosomes 0.000016 0.000016 0.000037 0.000050 0.000013 0.000015 0.000059 0.000015 0.000015 0.000016 0.000047 0.000019
Endoplasmic Reticulum 0.002620 0.002239 0.002712 0.001141 0.002642 0.002288 0.002498 0.000925 0.002629 0.002267 0.002615 0.000947
Endosome 0.000740 0.001064 0.000706 0.013037 0.000878 0.001080 0.000267 0.000325 0.000797 0.001073 0.000507 0.001644
Golgi 0.000125 0.000795 0.000253 0.011124 0.000286 0.000734 0.000172 0.000163 0.000192 0.000760 0.000216 0.001300
Lipid Particles 0.000267 0.000432 0.000545 0.040802 0.000336 0.000351 0.000428 0.000397 0.000296 0.000385 0.000492 0.004590
Mitochondria 0.001181 0.001525 0.002593 0.006729 0.001093 0.001632 0.002582 0.002450 0.001145 0.001587 0.002588 0.002894
Mitotic Spindle 0.008050 0.017138 0.004153 0.198275* 0.012571 0.016106 0.003284 0.078586 0.009936 0.016539 0.003758 0.091009
None 0.011804 0.013893 0.005085 0.042024 0.013418 0.015966 0.004530 0.009394 0.012477 0.015096 0.004833 0.012781
Nuclear Periphery 0.001525 0.000859 0.001658 0.001333 0.000960 0.001011 0.000976 0.001782 0.001289 0.000948 0.001349 0.001735
Nuclear Periphery Foci 0.001494 0.002745 0.001095 0.002227 0.002892 0.001826 0.000439 0.000691 0.002078 0.002212 0.000798 0.000851
Nucleolus 0.007793 0.005754 0.002376 0.001494 0.004908 0.006254 0.001977 0.001779 0.006589 0.006044 0.002195 0.001749
Nucleus 0.608689* 0.598772* 0.848274* 0.489282* 0.522930* 0.588889* 0.906972* 0.773407* 0.572903* 0.593037* 0.874925* 0.743918*
Peroxisomes 0.000303 0.000436 0.000331 0.003149 0.000342 0.000571 0.000532 0.000754 0.000319 0.000515 0.000423 0.001002
Vacuole 0.005200 0.006952 0.016308 0.021705 0.006189 0.006988 0.010156 0.006762 0.005612 0.006973 0.013515 0.008313
Vacuole Periphery 0.000170 0.000228 0.000199 0.000730 0.000325 0.000491 0.000122 0.000136 0.000235 0.000381 0.000164 0.000198

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -6.42 -8.17 -4.58 -2.66 0.01 -3.20 -6.68 -6.12 -1.63 4.54 -6.47 -10.37 -8.91 -2.45 5.31
Bud Neck -0.99 -0.44 0.46 0.94 0.67 -2.60 -2.90 -5.77 -3.49 -0.27 -1.95 -2.22 -4.41 -2.78 -0.76
Bud Site -5.59 -2.52 -4.04 -2.41 -2.51 -2.46 -0.34 -3.76 -1.54 -2.21 -6.06 -2.27 -6.85 -2.38 -2.78
Cell Periphery -7.19 -8.96 -6.30 -4.60 -2.28 0.34 -7.09 -10.26 -10.80 1.07 -5.07 -11.44 -13.36 -10.24 0.58
Cytoplasm 1.72 28.06 16.61 14.46 -1.95 9.82 40.76 38.61 31.36 -8.41 7.18 47.29 47.55 38.48 -5.05
Cytoplasmic Foci -4.12 2.29 -2.11 -1.23 -2.39 0.77 5.26 -0.38 -1.00 -4.39 -2.82 5.38 -2.73 -1.04 -5.25
Eisosomes -1.49 -6.86 -5.99 -5.67 -1.09 -2.92 -7.97 -10.43 -8.39 5.59 -2.73 -10.45 -11.71 -9.92 5.89
Endoplasmic Reticulum 2.16 -1.70 2.26 0.66 3.07 0.31 -1.45 6.29 6.26 5.30 1.88 -2.18 9.51 6.93 7.56
Endosome -0.72 -0.49 -1.27 -1.06 -1.18 -0.68 3.10 1.86 2.75 -2.34 -1.06 1.96 1.23 1.91 -0.42
Golgi -2.51 -5.48 -1.62 -0.78 -1.32 -2.50 -0.89 -0.58 2.42 0.58 -3.76 -4.00 -2.67 1.86 -0.80
Lipid Particles -3.23 -6.38 -2.27 -2.17 -1.96 -1.38 -3.99 -9.47 -9.00 -4.87 -3.36 -7.30 -7.12 -6.56 -4.62
Mitochondria -4.21 -10.30 -4.70 -3.45 0.39 -2.84 -11.06 -22.93 -12.78 -2.96 -4.14 -15.05 -24.21 -15.72 -3.85
Mitotic Spindle -3.28 -1.35 -5.01 -4.36 -4.40 -1.57 2.03 -12.39 -11.57 -12.84 -3.66 -0.16 -14.17 -12.49 -12.52
None -2.70 13.12 -0.75 -0.15 -3.29 -3.62 12.09 -0.69 2.84 -12.33 -5.21 17.84 -2.21 1.65 -13.97
Nuclear Periphery 4.50 -4.86 -6.81 -8.52 -4.01 -0.01 -4.99 -17.64 -16.57 -11.78 3.32 -6.61 -17.34 -19.14 -11.34
Nuclear Periphery Foci -1.40 1.02 1.63 2.92 -0.17 1.63 3.96 4.29 3.56 -0.70 0.31 2.85 5.36 5.54 0.12
Nucleolus 1.68 7.34 8.48 6.68 1.03 -3.96 2.38 2.71 6.62 -0.25 -0.99 7.39 7.23 8.07 -0.76
Nucleus 1.83 -12.29 1.93 1.28 7.65 -6.39 -21.33 -13.95 -8.57 10.59 -2.21 -22.85 -9.88 -8.08 11.84
Peroxisomes -2.17 -1.60 -1.70 -0.91 -1.11 -2.92 -2.10 -0.64 1.66 1.73 -3.65 -2.58 -0.96 1.38 1.51
Vacuole -2.92 -6.44 -8.05 -7.85 -5.53 -2.05 -4.39 -13.14 -12.08 -3.80 -3.57 -7.78 -15.37 -14.26 -4.15
Vacuole Periphery -2.37 -3.75 -2.08 -0.95 -0.36 -0.85 1.43 2.00 1.92 1.68 -1.47 -0.56 1.35 2.09 3.35