Standard name
Description Cyclin-dependent kinase (CDK) catalytic subunit; master regulator of mitotic and meiotic cell cycles; alternately associates with G1, S, G2/M phase cyclins, which provide substrate specificity; regulates metabolism, basal transcription, chromosome dynamics, growth and morphogenesis; transcript induction in osmostress involves antisense RNA; human homologs CDK1, CDK2, CDK3 can complement yeast conditional cdc28 mutants; human CDK1, CDK2 can complement yeast cdc28 null mutant

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0.09 0.05 0.05 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.55 0.49 0.56 0.48 0.37 0.53 0.5 0.41 0.54 0.47 0.53 0.46 0.42 0.46 0.44 0.54 0.5 0.44 0.37 0.33 0.39 0.62 0.75 0.77
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.14 0.07 0.08 0.14 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.12 0.18 0.19 0.21 0.24 0.18 0.19 0.16 0.17 0.18 0.15 0.17 0.23 0.24 0.34 0.18 0.14 0.14 0.35 0.41 0.34 0.19 0.12 0.11
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0.05 0 0.05 0.05 0 0.07 0 0.05 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.38 0.36 0.35 0.36 0.36 0.28 0.31 0.4 0.18 0.25 0.23 0.25 0.37 0.35 0.28 0.34 0.43 0.47 0 0.06 0.08 0.08 0.07 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 0 1 0 1 0 1 0 1 1 2 0 0 2 3 1 1 0 1 1 0 1 0 0
Bud 0 1 0 0 1 0 0 2 0 1 1 4 0 0 0 0 1 0 33 43 9 9 3 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Cell Periphery 0 2 0 0 0 0 2 2 2 2 6 1 0 0 0 1 0 0 0 0 0 0 0 0
Cytoplasm 157 202 104 62 91 148 197 172 158 154 144 150 40 81 97 70 72 64 118 155 76 111 172 161
Endoplasmic Reticulum 1 0 0 0 1 0 0 0 2 2 4 1 0 0 0 1 8 3 0 1 1 0 0 2
Endosome 1 1 0 1 2 3 3 3 1 2 2 2 1 6 3 1 0 2 5 7 4 1 2 2
Golgi 4 0 6 2 12 5 11 6 0 0 0 3 0 1 1 1 7 7 0 0 1 0 0 0
Mitochondria 1 2 0 0 0 4 3 5 42 22 23 45 0 0 0 3 0 0 0 1 3 0 2 2
Nucleus 34 74 35 27 59 51 73 66 49 60 42 57 22 43 75 24 20 20 112 192 66 33 27 23
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 3 2 0 1 0
Nucleolus 12 7 3 1 8 5 9 22 13 17 13 14 7 5 11 3 2 2 14 9 4 1 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 1 0 1 0 3 4 0 0 0 14 15 7 1 0 0
Vac/Vac Membrane 107 148 64 46 88 77 121 167 54 81 62 81 35 62 61 44 62 67 14 28 15 15 15 9
Unique Cell Count 283 410 185 129 245 278 392 416 295 327 272 328 95 177 220 130 144 144 322 467 198 180 229 209
Labelled Cell Count 319 437 213 139 263 293 420 445 322 343 300 359 105 204 256 149 173 165 322 467 198 180 229 209


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 16.9 11.3 14.9 14.4 15.3 13.2 13.5 12.8 11.3 11.9 11.7 11.6 13.7 14.8 15.6 13.9 18.0 18.3 14.3 14.7 13.8
Std Deviation (1e-4) 2.9 2.2 2.9 3.4 3.7 2.9 3.7 2.9 3.0 3.0 2.7 2.7 4.0 4.4 5.4 3.7 4.7 4.9 2.9 2.9 3.1
Intensity Change (Log2) -0.05 0.04 -0.17 -0.15 -0.22 -0.4 -0.33 -0.35 -0.36 -0.12 -0.01 0.07 -0.1 0.27 0.3 -0.06 -0.02 -0.11


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.4 -3.9 -0.6 -1.3 -3.4 -0.6 -2.0 -0.7 -2.3 -2.2 -2.0 -2.4 -0.4 -1.1 -2.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0.8 0 -0.3 0 0 0 0 0 0 0 0 0 0.7 0.7
Mitochondria 0 0 0 0 0 5.4 3.6 4.1 5.3 0 0 0 0 0 0
Nucleus 0.4 1.3 -0.2 -0.1 -0.9 -0.6 -0.2 -1.0 -0.4 0.8 1.2 3.4 -0.1 -1.2 -1.2
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 2.1 1.7 2.0 1.8 1.6 0 0 1.9 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0.2 0.3 -1.6 -0.9 1.3 -4.0 -2.4 -2.8 -2.4 0.4 0.1 -1.5 -0.1 1.6 2.2


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cdc28-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available