TheCellVision

SSE2

Standard name	SSE2 ORF YBR169C Aliases adenyl-nucleotide exchange factor SSE2
Human Ortholog	HSPA4L HSPH1 HSPA4
Description	Member of Hsp110 subclass of the heat shock protein 70 (HSP70) family; serves as nucleotide exchange factor to load ATP onto the SSA class of cytosolic Hsp70s; may be involved in protein folding; localized to the cytoplasm; SSE2 has a paralog, SSE1, that arose from the whole genome duplication

DOWNLOAD

Scale images

SSE2

Micrographs

Sub-cellular Localization

LOC-score
Cell count

	ensLOC																		DeepLoc
Localization	WT1	WT2	WT3	RAP60	RAP140	RAP220	RAP300	RAP380	RAP460	RAP540	RAP620	RAP700	HU80	HU120	HU160	rpd3Δ_1	rpd3Δ_2	rpd3Δ_3	WT1	WT2	WT3	AF100	AF140	AF180
Cortical Patches	0	0	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Bud	0	0	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Bud Neck	0	0	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Bud Site	0	0	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0	–	–	–	–	–	–
Cell Periphery	0	0	0.05	0	0	0	0	0	0	–	0.05	0.05	0	0	0	0.07	0.05	0.08	0	0	0	0	0	0
Cytoplasm	1.0	0.99	0.98	0.99	0.95	0.91	0.86	0.77	0.77	–	0.68	0.62	0.99	0.99	0.99	0.95	0.95	0.92	0.98	0.95	0.92	0.92	0.93	0.92
Endoplasmic Reticulum	0	0	0	0	0	0	0.1	0	0	–	0	0.07	0	0	0	0.05	0	0.05	0	0	0	0	0	0
Endosome	0	0	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Golgi	0	0	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Mitochondria	0	0	0	0	0	0	0	0	0.09	–	0.06	0	0	0	0	0	0	0	0	0	0	0	0	0
Nucleus	0	0	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Nuclear Periphery	0	0	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Nucleolus	0	0	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Peroxisomes	0	0	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0	0	0	0	0	0	0
SpindlePole	0	0	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Vac/Vac Membrane	0	0	0	0	0	0	0	0.17	0.13	–	0.23	0.28	0	0	0	0	0	0.07	0	0	0.05	0	0	0

	ensLOC																		DeepLoc
Localization	WT1	WT2	WT3	RAP60	RAP140	RAP220	RAP300	RAP380	RAP460	RAP540	RAP620	RAP700	HU80	HU120	HU160	rpd3Δ_1	rpd3Δ_2	rpd3Δ_3	WT1	WT2	WT3	AF100	AF140	AF180
Cortical Patches	0	0	1	0	1	1	3	1	2	–	1	9	0	0	0	0	0	0	0	0	0	0	1	0
Bud	1	1	5	0	2	4	4	2	7	–	5	5	1	0	0	0	0	0	0	1	1	0	2	5
Bud Neck	0	0	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0	0	0	0	0	0	1
Bud Site	0	0	0	0	0	0	0	0	0	–	0	1	0	0	0	0	0	0	–	–	–	–	–	–
Cell Periphery	1	2	15	3	14	19	19	18	15	–	26	37	2	4	5	22	8	11	0	0	0	0	0	0
Cytoplasm	220	275	294	294	400	426	451	439	328	–	323	437	183	312	342	302	138	128	210	269	287	124	283	364
Endoplasmic Reticulum	0	2	2	1	9	11	50	21	18	–	14	46	1	0	3	15	4	7	0	0	0	1	5	5
Endosome	0	0	0	0	0	0	0	1	0	–	0	0	0	0	0	0	0	0	0	2	0	0	0	0
Golgi	0	0	0	0	0	0	0	1	0	–	0	0	0	0	0	3	0	1	0	1	0	0	0	0
Mitochondria	0	1	1	0	2	21	14	2	37	–	30	14	1	0	1	7	0	0	0	1	4	1	2	4
Nucleus	0	2	6	5	4	4	4	8	7	–	5	7	0	1	0	0	1	0	0	1	0	0	0	0
Nuclear Periphery	0	0	0	0	0	0	0	0	1	–	3	2	0	0	0	0	0	0	0	0	0	0	0	0
Nucleolus	0	0	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Peroxisomes	0	0	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0	0	0	0	0	0	0
SpindlePole	0	0	0	0	0	0	0	0	1	–	0	0	0	0	0	0	0	0	0	1	0	0	0	0
Vac/Vac Membrane	0	0	1	0	6	15	22	99	54	–	111	194	0	2	0	9	6	10	0	1	14	5	4	8
Unique Cell Count	220	279	301	297	419	468	525	567	426		477	702	184	315	346	317	146	139	215	283	313	135	304	398
Labelled Cell Count	222	283	325	303	438	501	567	592	470		518	752	188	319	351	358	157	157	215	283	313	135	304	398

Yeast GFP Assignment

Cytoplasm

(Huh et al. Nature 2003 425:686)

Protein Abundance

Screen	WT1	WT2	WT3	RAP60	RAP140	RAP220	RAP300	RAP380	RAP460	RAP540	RAP620	RAP700	HU80	HU120	HU160	rpd3Δ_1	rpd3Δ_2	rpd3Δ_3	AF100	AF140	AF180
Mean Cell GFP Intensity (1e^-4)	8.5	8.4	7.7	6.8	9.2	8.9	10.4	12.2	11.2	–	11.9	14.6	8.3	11.0	13.1	23.4	27.2	22.4	7.2	7.8	9.2
Std Deviation (1e^-4)	2.1	2.1	1.4	1.1	2.6	2.0	2.6	3.3	3.6	–	4.2	5.3	1.8	3.3	4.0	8.4	11.3	7.7	1.3	1.6	2.0
Intensity Change (Log₂)	–	–	–	-0.19	0.25	0.2	0.43	0.65	0.53	–	0.63	0.91	0.1	0.51	0.76	1.6	1.81	1.53	-0.11	0.01	0.24

Localization Change

Localization	RAP60	RAP140	RAP220	RAP300	RAP380	RAP460	RAP540	RAP620	RAP700	HU80	HU120	HU160	rpd3Δ_1	rpd3Δ_2	rpd3Δ_3
Cortical Patches	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0
Bud	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0
Bud Neck	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0
Bud Site	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0
Cell Periphery	-2.8	-1.1	-0.6	-0.9	-1.3	-1.0	–	0.3	0.2	-2.3	-2.7	-2.6	1.0	0.2	1.2
Cytoplasm	1.3	-1.6	-3.7	-5.5	-7.8	-7.8	–	-10.0	-11.6	1.5	1.3	1.1	-1.6	-1.7	-2.8
Endoplasmic Reticulum	0	0	1.8	5.0	2.7	2.9	–	2.2	4.0	0	0	0	3.1	0	0
Endosome	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0
Golgi	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0
Mitochondria	0	0	3.4	2.4	0	5.0	–	4.1	0	0	0	0	0	0	0
Nucleus	-0.3	0	0	0	0	-0.4	–	0	0	0	0	0	0	0	0
Nuclear Periphery	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0
Nucleolus	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0
Peroxisomes	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0
SpindlePole	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0
Vacuole	0	0	2.7	3.2	7.5	6.2	–	8.9	10.0	0	0	0	0	0	0

External localization resources

dHITS | Loqate | YeastGFP | YeastRGB | YeastRC

Images

Protein Concentration and Protein Localization Data

	R1						R2						R3
	G1 Pre-START	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase	G1 Pre-START	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase	G1 Pre-START	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase
Concentration	1.8385	3.0759	2.262	2.3401	1.2034	3.0807	2.4917	3.4581	3.2692	2.7972	3.2115	3.5435	3.4178	4.6328	4.2298	7.7533	4.6753	3.9217
Actin	0.0142	0.0092	0.0106	0.0234	0.0241	0.024	0.0133	0.0021	0.0254	0.0142	0.0199	0.0058	0.0092	0.0057	0.0152	0.0014	0.0045	0.0009
Bud	0.0006	0.0025	0.0017	0.0006	0.0035	0.0005	0.0005	0.0006	0.0011	0.0108	0.0005	0.0009	0.0004	0.0016	0.0006	0.0018	0.0004	0.0001
Bud Neck	0.0021	0.0005	0.0036	0.0013	0.0015	0.0052	0.001	0.0002	0.0038	0.0006	0.0011	0.0011	0.0023	0.0001	0.0003	0.0013	0.0002	0.0006
Bud Periphery	0.0007	0.0022	0.0018	0.0006	0.005	0.0013	0.0005	0.0005	0.0013	0.0093	0.0005	0.0013	0.0005	0.0009	0.0006	0.0033	0.0006	0.0001
Bud Site	0.0022	0.013	0.0065	0.0017	0.003	0.0005	0.0024	0.0038	0.0073	0.0037	0.0013	0.0001	0.005	0.0011	0.001	0.0031	0.0003	0.0001
Cell Periphery	0.0002	0.0003	0.0005	0.0001	0.0002	0.0001	0.0002	0.0004	0.0003	0.0003	0.0001	0	0.0002	0.0001	0.0001	0.0001	0	0
Cytoplasm	0.7284	0.8835	0.807	0.7856	0.7009	0.8703	0.7884	0.9537	0.8678	0.8711	0.8361	0.9416	0.8702	0.9399	0.8741	0.8877	0.9405	0.9561
Cytoplasmic Foci	0.0253	0.009	0.0212	0.0641	0.0486	0.0176	0.0214	0.0026	0.0153	0.0139	0.0295	0.0096	0.0147	0.0024	0.0068	0.0251	0.0027	0.0067
Eisosomes	0.0001	0	0.0003	0.0003	0.0002	0.0001	0.0001	0	0.0001	0	0.0002	0	0.0001	0	0.0001	0	0.0001	0
Endoplasmic Reticulum	0.0105	0.0033	0.005	0.0104	0.0048	0.0029	0.0048	0.002	0.0034	0.0018	0.0029	0.0047	0.0055	0.002	0.0055	0.0021	0.0009	0.0021
Endosome	0.0341	0.0054	0.0188	0.0223	0.0293	0.0189	0.0528	0.001	0.0177	0.032	0.0381	0.0088	0.0103	0.0012	0.0147	0.0228	0.0019	0.0054
Golgi	0.0083	0.0006	0.0046	0.006	0.018	0.007	0.0166	0.0001	0.0098	0.0095	0.0193	0.0047	0.0033	0.0005	0.0156	0.0081	0.0011	0.0021
Lipid Particles	0.0039	0.0001	0.0049	0.0179	0.0147	0.0026	0.0177	0	0.0032	0.006	0.015	0.0041	0.0104	0.0001	0.0026	0.0013	0.0002	0.003
Mitochondria	0.0022	0.0006	0.0052	0.0126	0.0287	0.0021	0.0138	0.0002	0.0074	0.002	0.0037	0.0009	0.0029	0.0063	0.0176	0.0097	0.0424	0.0038
None	0.127	0.0628	0.0804	0.0116	0.0904	0.0345	0.0378	0.0299	0.0222	0.0155	0.0038	0.0079	0.045	0.0353	0.0303	0.0025	0.0014	0.0145
Nuclear Periphery	0.0196	0.0011	0.0019	0.0032	0.0022	0.0021	0.0046	0.0005	0.001	0.001	0.0021	0.0012	0.0031	0.0005	0.0046	0.0049	0.0003	0.0005
Nucleolus	0.0002	0.0002	0.0067	0.0004	0.0002	0.0001	0.0033	0	0.0002	0.0001	0.0003	0	0.0002	0	0.0003	0.0003	0	0
Nucleus	0.0108	0.0022	0.0046	0.0031	0.0024	0.0023	0.0032	0.0013	0.0014	0.0019	0.003	0.0016	0.0024	0.0008	0.0023	0.0025	0.001	0.0011
Peroxisomes	0.0019	0.0002	0.0046	0.0279	0.0166	0.003	0.0074	0	0.0083	0.001	0.0174	0.0029	0.0098	0.0003	0.0031	0.0073	0.0009	0.0019
Punctate Nuclear	0.0031	0.0006	0.0057	0.0045	0.0022	0.0012	0.0036	0.0003	0.0008	0.0011	0.0016	0.0017	0.0025	0.0001	0.0013	0.0006	0.0001	0.0003
Vacuole	0.0033	0.0025	0.0036	0.0018	0.0028	0.0029	0.0052	0.0007	0.0016	0.0027	0.0027	0.0007	0.0015	0.0007	0.0024	0.0082	0.0003	0.0005
Vacuole Periphery	0.0013	0.0003	0.0008	0.0007	0.0009	0.0008	0.0014	0.0001	0.0006	0.0015	0.0011	0.0002	0.0005	0.0001	0.0011	0.0061	0.0002	0.0002

Sequencing Data

	R1					R2
	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase
Gene Expression	16.4292	19.4243	13.7106	24.0924	17.1783	21.1872	29.3401	36.0488	38.9845	27.4153
Translational Efficiency	0.8997	1.0049	1.2889	1.0762	1.0892	1.0103	0.9934	0.9397	1.13	1.0185

Hit Data

Dataset	Hit
Protein Concentration	✘
Protein Localization	✘
Gene Expression	✘
Translational Efficiency	✔

Proteome Dynamics

Micrographs

Cell Count

R1				R2				R1 & R2
WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14
1770	773	532	538	1444	536	1061	248	3214	1309	1593	786

Protein Abundance

	R1				R2				R1 & R2
	WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14
Mean	810.42	974.08	2175.61	2197.20	901.04	894.11	1530.44	2330.59	851.13	941.33	1745.90	2239.29
Standard Deviation	130.26	174.89	503.26	716.72	136.87	198.95	420.91	763.67	140.69	189.25	543.29	734.48
Intensity Change Log ₂	—	0.265370	1.424678	1.438925	—	-0.011139	0.764283	1.371032	—	0.126414	1.114655	1.403579

Localization Score (DeepLoc)

	R1				R2				R1 & R2
	WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14
Actin	0.000234	0.000716	0.000550	0.000612	0.000108	0.000263	0.000286	0.002294	0.000178	0.000530	0.000374	0.001143
Bud Neck	0.003639	0.003638	0.004443	0.003983	0.003043	0.005451	0.001541	0.003834	0.003371	0.004380	0.002510	0.003936
Bud Site	0.010603	0.021026	0.008701	0.025167	0.007031	0.019564	0.006358	0.012191	0.008998	0.020427	0.007141	0.021073
Cell Periphery	0.000111	0.000117	0.001890	0.001344	0.000108	0.000101	0.000165	0.001612	0.000110	0.000111	0.000741	0.001428
Cytoplasm	0.728783*	0.726810*	0.785807*	0.778674*	0.686467*	0.664481*	0.799400*	0.733051*	0.709771*	0.701288*	0.794861*	0.764279*
Cytoplasmic Foci	0.134377	0.129736	0.023279	0.016784	0.139998	0.157067	0.025775	0.030814	0.136903	0.140927	0.024942	0.021211
Eisosomes	0.000040	0.000047	0.000004	0.000009	0.000036	0.000041	0.000008	0.000032	0.000038	0.000045	0.000007	0.000016
Endoplasmic Reticulum	0.001210	0.000724	0.000244	0.000793	0.002818	0.000507	0.000538	0.001103	0.001932	0.000635	0.000440	0.000891
Endosome	0.008691	0.013282	0.001029	0.010791	0.009365	0.020076	0.002247	0.012321	0.008994	0.016064	0.001841	0.011274
Golgi	0.003154	0.002365	0.002830	0.010821	0.001729	0.001794	0.000351	0.013984	0.002514	0.002131	0.001179	0.011819
Lipid Particles	0.001806	0.000926	0.006007	0.010504	0.002104	0.001993	0.001611	0.013891	0.001940	0.001363	0.003079	0.011572
Mitochondria	0.001817	0.001175	0.000110	0.000483	0.000651	0.002092	0.000078	0.000611	0.001293	0.001550	0.000089	0.000523
Mitotic Spindle	0.000910	0.002061	0.003557	0.010449	0.000696	0.000989	0.013961	0.001938	0.000814	0.001622	0.010486	0.007764
None	0.005108	0.001151	0.004941	0.001757	0.003663	0.001714	0.002604	0.001146	0.004459	0.001382	0.003385	0.001564
Nuclear Periphery	0.000118	0.000148	0.000312	0.000595	0.000246	0.000139	0.000302	0.000155	0.000175	0.000145	0.000305	0.000457
Nuclear Periphery Foci	0.001224	0.001841	0.005361	0.004945	0.001462	0.001854	0.005683	0.007514	0.001331	0.001846	0.005575	0.005755
Nucleolus	0.000651	0.000222	0.000126	0.000193	0.000369	0.001263	0.000124	0.000126	0.000524	0.000648	0.000124	0.000172
Nucleus	0.034310	0.017216	0.112530	0.018646	0.046797	0.024548	0.051809	0.025938	0.039920	0.020218	0.072087	0.020947
Peroxisomes	0.001109	0.002334	0.000088	0.001306	0.000669	0.002434	0.000137	0.000336	0.000912	0.002375	0.000120	0.001000
Vacuole	0.060490	0.073352	0.037688	0.096328	0.091301	0.090932	0.086823	0.134112	0.074333	0.080550	0.070414	0.108249
Vacuole Periphery	0.001614	0.001113	0.000503	0.005817	0.001338	0.002697	0.000199	0.002996	0.001490	0.001761	0.000301	0.004927

Localization Changes (T-score)

	R1					R2					R1 & R2
	UBP2_WT	UBP14_WT	UBP2UBP14_WT	UBP2UBP14_UBP2	UBP2UBP14_UBP14	UBP2_WT	UBP14_WT	UBP2UBP14_WT	UBP2UBP14_UBP2	UBP2UBP14_UBP14	UBP2_WT	UBP14_WT	UBP2UBP14_WT	UBP2UBP14_UBP2	UBP2UBP14_UBP14
Actin	-1.31	-6.40	-2.36	0.68	3.75	-3.20	-9.52	-1.45	-1.09	-0.85	-1.65	-7.84	-2.43	-0.13	-0.13
Bud Neck	0.23	-5.36	-2.48	-2.49	2.67	-2.48	0.92	-3.74	0.20	-4.48	-1.82	-2.57	-4.10	-1.48	-2.22
Bud Site	-4.71	-0.21	-2.71	0.85	-2.36	-5.68	-1.84	-4.12	3.31	-3.33	-7.25	-0.65	-3.80	1.79	-3.54
Cell Periphery	-0.20	-12.31	-6.03	-5.98	5.00	0.74	-5.88	-6.03	-6.08	-5.06	0.08	-12.41	-7.82	-7.80	-0.88
Cytoplasm	0.44	0.83	0.17	-0.17	-0.45	1.77	-2.00	1.99	0.67	2.98	1.28	-0.11	0.70	-0.26	0.71
Cytoplasmic Foci	0.88	25.19	29.47	19.54	4.22	-2.09	25.70	22.92	17.82	0.68	-0.64	36.54	38.09	26.67	3.97
Eisosomes	-0.28	14.76	13.56	1.95	-0.79	-1.47	14.17	4.17	4.65	-0.44	-0.47	19.55	12.64	2.93	-0.08
Endoplasmic Reticulum	1.86	3.43	3.06	2.04	0.12	5.14	5.19	5.16	1.04	0.99	5.18	6.10	5.94	2.22	1.01
Endosome	-2.41	12.08	3.18	4.19	-2.19	-4.93	12.22	2.72	5.59	-0.60	-5.00	16.41	4.28	6.96	-1.81
Golgi	1.83	-0.18	-0.50	-1.01	-0.46	-0.04	8.97	-0.96	-0.94	-1.57	1.49	6.49	-1.06	-1.39	-1.94
Lipid Particles	3.86	-8.90	-5.82	-6.47	-0.82	0.28	-2.31	-5.95	-5.79	-5.44	2.39	-8.40	-7.82	-8.26	-5.02
Mitochondria	0.79	3.07	2.93	1.29	-0.58	-1.19	3.53	2.63	1.62	-0.73	-0.26	3.83	3.50	2.06	-1.04
Mitotic Spindle	-1.18	-5.02	-3.49	-2.83	-1.54	-0.60	-7.31	-2.09	-2.03	2.30	-1.32	-8.04	-4.08	-3.57	2.01
None	5.85	1.47	5.36	-0.94	7.00	2.47	1.89	3.07	0.97	3.92	6.02	3.16	5.99	-0.07	6.87
Nuclear Periphery	-0.37	-8.71	-1.63	-1.30	0.95	1.86	-4.81	-0.28	-3.26	6.67	0.54	-9.65	-1.49	-1.67	1.78
Nuclear Periphery Foci	-3.30	-12.40	-4.41	-3.33	3.80	-1.58	-11.12	-4.64	-4.11	0.53	-3.46	-16.15	-6.13	-5.01	3.07
Nucleolus	2.01	2.41	2.40	1.09	-0.05	-1.80	6.54	7.35	2.34	2.04	-0.41	3.23	3.28	2.48	0.46
Nucleus	10.32	-18.19	4.03	-4.51	19.38	7.95	-5.65	5.16	-1.45	9.05	12.95	-17.19	7.24	-3.83	20.58
Peroxisomes	-3.06	7.94	1.71	3.69	-1.68	-4.63	6.62	5.05	5.88	-1.76	-5.22	10.03	2.10	5.69	-1.70
Vacuole	-3.51	-0.56	-11.07	-8.47	-9.74	-0.35	-8.77	-9.05	-8.55	-4.85	-2.46	-9.92	-13.58	-11.64	-6.70
Vacuole Periphery	2.02	4.99	-0.92	-1.63	-2.35	-1.48	7.67	0.85	1.74	-1.58	-0.53	8.83	-0.73	-0.31	-2.92