Standard name
Description Member of Hsp110 subclass of the heat shock protein 70 (HSP70) family; serves as nucleotide exchange factor to load ATP onto the SSA class of cytosolic Hsp70s; may be involved in protein folding; localized to the cytoplasm; SSE2 has a paralog, SSE1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0.05 0 0 0 0 0 0 – 0.05 0.05 0 0 0 0.07 0.05 0.08 0 0 0 0 0 0
Cytoplasm 1.0 0.99 0.98 0.99 0.95 0.91 0.86 0.77 0.77 – 0.68 0.62 0.99 0.99 0.99 0.95 0.95 0.92 0.98 0.95 0.92 0.92 0.93 0.92
Endoplasmic Reticulum 0 0 0 0 0 0 0.1 0 0 – 0 0.07 0 0 0 0.05 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.09 – 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.17 0.13 – 0.23 0.28 0 0 0 0 0 0.07 0 0 0.05 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 1 1 3 1 2 – 1 9 0 0 0 0 0 0 0 0 0 0 1 0
Bud 1 1 5 0 2 4 4 2 7 – 5 5 1 0 0 0 0 0 0 1 1 0 2 5
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 0 0 0 – 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 2 15 3 14 19 19 18 15 – 26 37 2 4 5 22 8 11 0 0 0 0 0 0
Cytoplasm 220 275 294 294 400 426 451 439 328 – 323 437 183 312 342 302 138 128 210 269 287 124 283 364
Endoplasmic Reticulum 0 2 2 1 9 11 50 21 18 – 14 46 1 0 3 15 4 7 0 0 0 1 5 5
Endosome 0 0 0 0 0 0 0 1 0 – 0 0 0 0 0 0 0 0 0 2 0 0 0 0
Golgi 0 0 0 0 0 0 0 1 0 – 0 0 0 0 0 3 0 1 0 1 0 0 0 0
Mitochondria 0 1 1 0 2 21 14 2 37 – 30 14 1 0 1 7 0 0 0 1 4 1 2 4
Nucleus 0 2 6 5 4 4 4 8 7 – 5 7 0 1 0 0 1 0 0 1 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 1 – 3 2 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 1 – 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Vac/Vac Membrane 0 0 1 0 6 15 22 99 54 – 111 194 0 2 0 9 6 10 0 1 14 5 4 8
Unique Cell Count 220 279 301 297 419 468 525 567 426 477 702 184 315 346 317 146 139 215 283 313 135 304 398
Labelled Cell Count 222 283 325 303 438 501 567 592 470 518 752 188 319 351 358 157 157 215 283 313 135 304 398


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.5 8.4 7.7 6.8 9.2 8.9 10.4 12.2 11.2 – 11.9 14.6 8.3 11.0 13.1 23.4 27.2 22.4 7.2 7.8 9.2
Std Deviation (1e-4) 2.1 2.1 1.4 1.1 2.6 2.0 2.6 3.3 3.6 – 4.2 5.3 1.8 3.3 4.0 8.4 11.3 7.7 1.3 1.6 2.0
Intensity Change (Log2) – – – -0.19 0.25 0.2 0.43 0.65 0.53 – 0.63 0.91 0.1 0.51 0.76 1.6 1.81 1.53 -0.11 0.01 0.24


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cell Periphery -2.8 -1.1 -0.6 -0.9 -1.3 -1.0 – 0.3 0.2 -2.3 -2.7 -2.6 1.0 0.2 1.2
Cytoplasm 1.3 -1.6 -3.7 -5.5 -7.8 -7.8 – -10.0 -11.6 1.5 1.3 1.1 -1.6 -1.7 -2.8
Endoplasmic Reticulum 0 0 1.8 5.0 2.7 2.9 – 2.2 4.0 0 0 0 3.1 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0 0 3.4 2.4 0 5.0 – 4.1 0 0 0 0 0 0 0
Nucleus -0.3 0 0 0 0 -0.4 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vacuole 0 0 2.7 3.2 7.5 6.2 – 8.9 10.0 0 0 0 0 0 0