Standard name | |
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Description | Member of Hsp110 subclass of the heat shock protein 70 (HSP70) family; serves as nucleotide exchange factor to load ATP onto the SSA class of cytosolic Hsp70s; may be involved in protein folding; localized to the cytoplasm; SSE2 has a paralog, SSE1, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 1 | 0 | 1 | 1 | 3 | 1 | 2 | – | 1 | 9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Bud | 1 | 1 | 5 | 0 | 2 | 4 | 4 | 2 | 7 | – | 5 | 5 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 2 | 5 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 2 | 15 | 3 | 14 | 19 | 19 | 18 | 15 | – | 26 | 37 | 2 | 4 | 5 | 22 | 8 | 11 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 220 | 275 | 294 | 294 | 400 | 426 | 451 | 439 | 328 | – | 323 | 437 | 183 | 312 | 342 | 302 | 138 | 128 | 210 | 269 | 287 | 124 | 283 | 364 |
Endoplasmic Reticulum | 0 | 2 | 2 | 1 | 9 | 11 | 50 | 21 | 18 | – | 14 | 46 | 1 | 0 | 3 | 15 | 4 | 7 | 0 | 0 | 0 | 1 | 5 | 5 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 1 | 1 | 0 | 2 | 21 | 14 | 2 | 37 | – | 30 | 14 | 1 | 0 | 1 | 7 | 0 | 0 | 0 | 1 | 4 | 1 | 2 | 4 |
Nucleus | 0 | 2 | 6 | 5 | 4 | 4 | 4 | 8 | 7 | – | 5 | 7 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 3 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 1 | 0 | 6 | 15 | 22 | 99 | 54 | – | 111 | 194 | 0 | 2 | 0 | 9 | 6 | 10 | 0 | 1 | 14 | 5 | 4 | 8 |
Unique Cell Count | 220 | 279 | 301 | 297 | 419 | 468 | 525 | 567 | 426 | 477 | 702 | 184 | 315 | 346 | 317 | 146 | 139 | 215 | 283 | 313 | 135 | 304 | 398 | |
Labelled Cell Count | 222 | 283 | 325 | 303 | 438 | 501 | 567 | 592 | 470 | 518 | 752 | 188 | 319 | 351 | 358 | 157 | 157 | 215 | 283 | 313 | 135 | 304 | 398 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 8.5 | 8.4 | 7.7 | 6.8 | 9.2 | 8.9 | 10.4 | 12.2 | 11.2 | – | 11.9 | 14.6 | 8.3 | 11.0 | 13.1 | 23.4 | 27.2 | 22.4 | 7.2 | 7.8 | 9.2 |
Std Deviation (1e-4) | 2.1 | 2.1 | 1.4 | 1.1 | 2.6 | 2.0 | 2.6 | 3.3 | 3.6 | – | 4.2 | 5.3 | 1.8 | 3.3 | 4.0 | 8.4 | 11.3 | 7.7 | 1.3 | 1.6 | 2.0 |
Intensity Change (Log2) | – | – | – | -0.19 | 0.25 | 0.2 | 0.43 | 0.65 | 0.53 | – | 0.63 | 0.91 | 0.1 | 0.51 | 0.76 | 1.6 | 1.81 | 1.53 | -0.11 | 0.01 | 0.24 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -2.8 | -1.1 | -0.6 | -0.9 | -1.3 | -1.0 | – | 0.3 | 0.2 | -2.3 | -2.7 | -2.6 | 1.0 | 0.2 | 1.2 |
Cytoplasm | 1.3 | -1.6 | -3.7 | -5.5 | -7.8 | -7.8 | – | -10.0 | -11.6 | 1.5 | 1.3 | 1.1 | -1.6 | -1.7 | -2.8 |
Endoplasmic Reticulum | 0 | 0 | 1.8 | 5.0 | 2.7 | 2.9 | – | 2.2 | 4.0 | 0 | 0 | 0 | 3.1 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 3.4 | 2.4 | 0 | 5.0 | – | 4.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | -0.3 | 0 | 0 | 0 | 0 | -0.4 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 2.7 | 3.2 | 7.5 | 6.2 | – | 8.9 | 10.0 | 0 | 0 | 0 | 0 | 0 | 0 |