Standard name | |
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Description | Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S6, no bacterial homolog; phosphorylated on S233 by Ypk3p in a TORC1-dependent manner, and on S232 in a TORC1/2-dependent manner by Ypk1/2/3p; RPS6B has a paralog, RPS6A, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 1 | 2 | 1 | 9 | 9 | 9 | 6 | 5 | 0 | – | 1 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 4 | 4 | 4 | 6 | 12 | 10 | 6 | 9 | 5 | 0 | – | 4 | 2 | 18 | 2 | 8 | 0 | 0 | 0 | 0 | 0 | 1 |
Cytoplasm | 146 | 133 | 108 | 124 | 129 | 102 | 163 | 128 | 88 | 105 | 58 | 40 | – | 118 | 114 | 250 | 199 | 264 | 142 | 151 | 116 | 96 | 94 | 149 |
Endoplasmic Reticulum | 3 | 1 | 6 | 7 | 10 | 6 | 23 | 40 | 15 | 20 | 9 | 5 | – | 1 | 4 | 8 | 8 | 10 | 3 | 2 | 5 | 11 | 14 | 11 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Golgi | 0 | 0 | 0 | 1 | 3 | 2 | 4 | 2 | 3 | 3 | 0 | 2 | – | 0 | 0 | 2 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 1 | 0 | 6 | 0 | 17 | 37 | 30 | 60 | 52 | 53 | 27 | – | 0 | 0 | 0 | 4 | 8 | 2 | 0 | 1 | 0 | 5 | 3 |
Nucleus | 0 | 0 | 0 | 1 | 1 | 2 | 2 | 2 | 0 | 1 | 0 | 2 | – | 0 | 2 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 4 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 1 | 0 | 5 | 7 | 4 | 10 | 6 | 5 | 12 | 5 | 5 | – | 1 | 0 | 12 | 13 | 4 | 0 | 3 | 2 | 1 | 2 | 2 |
Unique Cell Count | 149 | 136 | 114 | 137 | 149 | 123 | 223 | 199 | 154 | 179 | 119 | 75 | 122 | 116 | 270 | 213 | 274 | 153 | 158 | 128 | 114 | 121 | 173 | |
Labelled Cell Count | 149 | 137 | 118 | 149 | 156 | 140 | 263 | 228 | 187 | 209 | 140 | 82 | 125 | 122 | 292 | 232 | 295 | 153 | 158 | 128 | 114 | 121 | 173 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 15.8 | 22.8 | 15.4 | 17.0 | 16.6 | 13.4 | 12.9 | 12.7 | 11.8 | 11.9 | 11.1 | 10.8 | – | 18.9 | 18.2 | 24.3 | 24.5 | 25.4 | 18.0 | 15.6 | 18.0 |
Std Deviation (1e-4) | 2.9 | 4.0 | 2.5 | 2.9 | 3.0 | 3.1 | 3.1 | 2.8 | 2.5 | 2.8 | 2.7 | 2.4 | – | 3.2 | 3.6 | 6.1 | 6.1 | 6.4 | 4.2 | 4.0 | 4.4 |
Intensity Change (Log2) | – | – | – | 0.14 | 0.11 | -0.2 | -0.25 | -0.28 | -0.38 | -0.37 | -0.47 | -0.5 | – | 0.3 | 0.25 | 0.66 | 0.67 | 0.73 | 0.23 | 0.02 | 0.23 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0.8 | 0.6 | 0 | 0.6 | 0 | 0 | – | 0 | 0 | 1.2 | 0 | 0 |
Cytoplasm | -1.3 | -2.2 | -2.9 | -4.7 | -6.0 | -6.9 | -6.8 | -7.8 | -6.8 | – | 0.8 | 1.5 | -0.8 | -0.5 | 0.7 |
Endoplasmic Reticulum | -0.1 | 0.5 | -0.1 | 1.6 | 3.6 | 1.3 | 1.7 | 0.7 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 4.1 | 4.6 | 4.4 | 7.6 | 6.3 | 8.1 | 6.9 | – | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |