Standard name
Human Ortholog
Description Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S6, no bacterial homolog; phosphorylated on S233 by Ypk3p in a TORC1-dependent manner, and on S232 in a TORC1/2-dependent manner by Ypk1/2/3p; RPS6B has a paralog, RPS6A, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0.05 0.06 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0.05 0.05 0.05 0 0.05 0 0 – 0 0 0.07 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.98 0.95 0.91 0.87 0.83 0.73 0.64 0.57 0.59 0.49 0.53 – 0.97 0.98 0.93 0.93 0.96 0.93 0.96 0.91 0.84 0.78 0.86
Endoplasmic Reticulum 0 0 0.05 0.05 0.07 0.05 0.1 0.2 0.1 0.11 0.08 0.07 – 0 0 0 0 0 0 0 0 0.1 0.12 0.07
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.14 0.17 0.15 0.39 0.29 0.45 0.36 – 0 0 0 0 0 0 0 0 0 0.05 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.05 0 0 0 0 0.07 0 0.07 – 0 0 0 0.06 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 2 1 9 9 9 6 5 0 – 1 0 1 1 1 0 0 0 0 0 0
Bud 0 0 0 0 0 0 2 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 1 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 4 4 4 6 12 10 6 9 5 0 – 4 2 18 2 8 0 0 0 0 0 1
Cytoplasm 146 133 108 124 129 102 163 128 88 105 58 40 – 118 114 250 199 264 142 151 116 96 94 149
Endoplasmic Reticulum 3 1 6 7 10 6 23 40 15 20 9 5 – 1 4 8 8 10 3 2 5 11 14 11
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 1
Golgi 0 0 0 1 3 2 4 2 3 3 0 2 – 0 0 2 4 0 0 0 0 0 0 0
Mitochondria 0 1 0 6 0 17 37 30 60 52 53 27 – 0 0 0 4 8 2 0 1 0 5 3
Nucleus 0 0 0 1 1 2 2 2 0 1 0 2 – 0 2 1 1 0 1 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 1 1 1 0 4 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 1 1 1 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 1
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 1 0 5 7 4 10 6 5 12 5 5 – 1 0 12 13 4 0 3 2 1 2 2
Unique Cell Count 149 136 114 137 149 123 223 199 154 179 119 75 122 116 270 213 274 153 158 128 114 121 173
Labelled Cell Count 149 137 118 149 156 140 263 228 187 209 140 82 125 122 292 232 295 153 158 128 114 121 173


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 15.8 22.8 15.4 17.0 16.6 13.4 12.9 12.7 11.8 11.9 11.1 10.8 – 18.9 18.2 24.3 24.5 25.4 18.0 15.6 18.0
Std Deviation (1e-4) 2.9 4.0 2.5 2.9 3.0 3.1 3.1 2.8 2.5 2.8 2.7 2.4 – 3.2 3.6 6.1 6.1 6.4 4.2 4.0 4.4
Intensity Change (Log2) – – – 0.14 0.11 -0.2 -0.25 -0.28 -0.38 -0.37 -0.47 -0.5 – 0.3 0.25 0.66 0.67 0.73 0.23 0.02 0.23


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Cell Periphery 0 0 0 0.8 0.6 0 0.6 0 0 – 0 0 1.2 0 0
Cytoplasm -1.3 -2.2 -2.9 -4.7 -6.0 -6.9 -6.8 -7.8 -6.8 – 0.8 1.5 -0.8 -0.5 0.7
Endoplasmic Reticulum -0.1 0.5 -0.1 1.6 3.6 1.3 1.7 0.7 0 – 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Mitochondria 0 0 4.1 4.6 4.4 7.6 6.3 8.1 6.9 – 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 26.5211 34.9736 32.25 31.2431 25.0575 32.0047 27.232 33.8451 31.8206 27.9101 29.4427 30.8481 28.2795 37.4315 32.7429 23.9491 28.6654 32.9587
Actin 0.0046 0.0015 0.0004 0.001 0.0044 0.0008 0.004 0.0011 0.0005 0.0003 0.0005 0.0016 0.0013 0.0038 0.0019 0.005 0.0022 0.0022
Bud 0.0012 0.0005 0.0006 0.0051 0.0031 0.0008 0.0008 0.0004 0.0006 0.0009 0.0009 0.0012 0.005 0.006 0.0068 0.0203 0.009 0.0044
Bud Neck 0.0011 0.0004 0.0006 0.0008 0.0009 0.0013 0.0006 0.0004 0.0007 0.0012 0.0011 0.0015 0.0014 0.0012 0.0016 0.0026 0.0028 0.0048
Bud Periphery 0.0022 0.0008 0.0004 0.0227 0.0061 0.0008 0.0011 0.0002 0.0007 0.0004 0.0008 0.0018 0.0087 0.0042 0.0092 0.0224 0.0155 0.0097
Bud Site 0.001 0.0006 0.0004 0.0007 0.002 0.0001 0.0005 0.0004 0.0003 0.0002 0.0001 0.0001 0.0024 0.0074 0.005 0.0036 0.0016 0.0005
Cell Periphery 0.0041 0.0031 0.0014 0.0018 0.0053 0.0009 0.0047 0.0006 0.0012 0.0009 0.0005 0.0022 0.0073 0.0035 0.0103 0.0048 0.0031 0.0054
Cytoplasm 0.7342 0.9292 0.8731 0.8014 0.7014 0.8285 0.7535 0.9226 0.8729 0.8707 0.8226 0.7932 0.6743 0.8527 0.727 0.5542 0.7133 0.6935
Cytoplasmic Foci 0.0073 0.0034 0.0095 0.0054 0.0257 0.0095 0.0057 0.0052 0.0066 0.0097 0.0136 0.0108 0.004 0.0031 0.0042 0.0046 0.0089 0.0068
Eisosomes 0.0001 0.0001 0 0 0.0005 0 0.0001 0 0 0 0 0 0 0.0001 0 0 0 0.0001
Endoplasmic Reticulum 0.0823 0.0244 0.0223 0.0084 0.0277 0.0707 0.1063 0.0319 0.0378 0.0175 0.0474 0.0875 0.1042 0.0397 0.045 0.0394 0.036 0.1188
Endosome 0.0367 0.0075 0.0244 0.0232 0.0536 0.0411 0.0322 0.0132 0.0221 0.0392 0.067 0.0439 0.0132 0.0072 0.0086 0.017 0.0346 0.0294
Golgi 0.0057 0.0022 0.0023 0.0028 0.0049 0.0069 0.0055 0.0046 0.0024 0.0038 0.0092 0.0132 0.0016 0.0021 0.0018 0.001 0.0035 0.0073
Lipid Particles 0.0013 0.0004 0.0014 0.0004 0.0118 0.0002 0.0006 0.0002 0.0003 0.0002 0.0001 0.0002 0.001 0.0004 0.0015 0.0009 0.0012 0.0004
Mitochondria 0.011 0.0045 0.0021 0.0126 0.0172 0.007 0.0051 0.0017 0.0016 0.0005 0.0059 0.0175 0.0032 0.001 0.0025 0.0019 0.0018 0.0113
None 0.0038 0.0015 0.003 0.0009 0.0158 0.0006 0.0008 0.0002 0.0003 0.0002 0.0003 0.0006 0.0012 0.0006 0.0016 0.0013 0.0014 0.0007
Nuclear Periphery 0.0162 0.0017 0.0042 0.0036 0.0166 0.0035 0.0113 0.0014 0.003 0.003 0.0021 0.0038 0.016 0.0054 0.0111 0.0261 0.0179 0.0095
Nucleolus 0.0013 0.0001 0.0018 0.0003 0.0022 0 0.0001 0.0001 0 0 0 0 0.001 0.0007 0.0011 0.0037 0.0021 0.001
Nucleus 0.007 0.0012 0.0044 0.0025 0.0082 0.0015 0.003 0.0018 0.0013 0.0018 0.0012 0.0012 0.0765 0.0322 0.0855 0.1284 0.0647 0.046
Peroxisomes 0.0007 0.0001 0.0002 0.0003 0.0007 0.0001 0.0006 0 0 0.0001 0.0001 0.0001 0.0007 0.0001 0.0001 0.0001 0.0001 0.0001
Punctate Nuclear 0.0022 0.0001 0.001 0.0001 0.0067 0.0004 0.0001 0 0.0001 0 0 0.0001 0.0058 0.0018 0.0101 0.0078 0.0083 0.0062
Vacuole 0.063 0.0147 0.0377 0.0911 0.0627 0.0186 0.054 0.0129 0.042 0.0436 0.0202 0.015 0.0615 0.0247 0.0591 0.141 0.0627 0.0335
Vacuole Periphery 0.0132 0.0021 0.0087 0.015 0.0225 0.0067 0.0095 0.0012 0.0057 0.0057 0.0065 0.0044 0.0094 0.0021 0.0059 0.0136 0.0093 0.0083

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 3217.099 3609.7761 4404.9848 3396.6013 4039.3161 3894.6761 3130.8397 2957.1173 3409.6389 2838.0761
Translational Efficiency 0.9071 0.9111 0.789 1.0834 0.9361 0.7032 0.8985 1.0558 0.8812 0.9719

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S6, no bacterial homolog; phosphorylated on S233 by Ypk3p in a TORC1-dependent manner, and on S232 in a TORC1/2-dependent manner by Ypk1/2/3p; RPS6B has a paralog, RPS6A, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (97%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Rps6b

Rps6b


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rps6b-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available