Standard name
Description Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S6, no bacterial homolog; phosphorylated on S233 by Ypk3p in a TORC1-dependent manner, and on S232 in a TORC1/2-dependent manner by Ypk1/2/3p; RPS6B has a paralog, RPS6A, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0.05 0.06 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0.05 0.05 0.05 0 0.05 0 0 – 0 0 0.07 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.98 0.95 0.91 0.87 0.83 0.73 0.64 0.57 0.59 0.49 0.53 – 0.97 0.98 0.93 0.93 0.96 0.93 0.96 0.91 0.84 0.78 0.86
Endoplasmic Reticulum 0 0 0.05 0.05 0.07 0.05 0.1 0.2 0.1 0.11 0.08 0.07 – 0 0 0 0 0 0 0 0 0.1 0.12 0.07
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.14 0.17 0.15 0.39 0.29 0.45 0.36 – 0 0 0 0 0 0 0 0 0 0.05 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.05 0 0 0 0 0.07 0 0.07 – 0 0 0 0.06 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 2 1 9 9 9 6 5 0 – 1 0 1 1 1 0 0 0 0 0 0
Bud 0 0 0 0 0 0 2 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 1 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 4 4 4 6 12 10 6 9 5 0 – 4 2 18 2 8 0 0 0 0 0 1
Cytoplasm 146 133 108 124 129 102 163 128 88 105 58 40 – 118 114 250 199 264 142 151 116 96 94 149
Endoplasmic Reticulum 3 1 6 7 10 6 23 40 15 20 9 5 – 1 4 8 8 10 3 2 5 11 14 11
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 1
Golgi 0 0 0 1 3 2 4 2 3 3 0 2 – 0 0 2 4 0 0 0 0 0 0 0
Mitochondria 0 1 0 6 0 17 37 30 60 52 53 27 – 0 0 0 4 8 2 0 1 0 5 3
Nucleus 0 0 0 1 1 2 2 2 0 1 0 2 – 0 2 1 1 0 1 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 1 1 1 0 4 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 1 1 1 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 1
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 1 0 5 7 4 10 6 5 12 5 5 – 1 0 12 13 4 0 3 2 1 2 2
Unique Cell Count 149 136 114 137 149 123 223 199 154 179 119 75 122 116 270 213 274 153 158 128 114 121 173
Labelled Cell Count 149 137 118 149 156 140 263 228 187 209 140 82 125 122 292 232 295 153 158 128 114 121 173


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 15.8 22.8 15.4 17.0 16.6 13.4 12.9 12.7 11.8 11.9 11.1 10.8 – 18.9 18.2 24.3 24.5 25.4 18.0 15.6 18.0
Std Deviation (1e-4) 2.9 4.0 2.5 2.9 3.0 3.1 3.1 2.8 2.5 2.8 2.7 2.4 – 3.2 3.6 6.1 6.1 6.4 4.2 4.0 4.4
Intensity Change (Log2) – – – 0.14 0.11 -0.2 -0.25 -0.28 -0.38 -0.37 -0.47 -0.5 – 0.3 0.25 0.66 0.67 0.73 0.23 0.02 0.23


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Cell Periphery 0 0 0 0.8 0.6 0 0.6 0 0 – 0 0 1.2 0 0
Cytoplasm -1.3 -2.2 -2.9 -4.7 -6.0 -6.9 -6.8 -7.8 -6.8 – 0.8 1.5 -0.8 -0.5 0.7
Endoplasmic Reticulum -0.1 0.5 -0.1 1.6 3.6 1.3 1.7 0.7 0 – 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Mitochondria 0 0 4.1 4.6 4.4 7.6 6.3 8.1 6.9 – 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0