Standard name
Human Ortholog
Description Calcium and manganese transporter with higher affinity for Ca2+; involved in Ca2+ and Mn2+ homeostasis; localizes to the cis- and medial-Golgi apparatus; GFP-fusion localizes to the vacuole; required for the Mn2+-dependent function of glycosylation enzymes; TMEM165, a human ortholog linked to Congenital Disorders of Glycosylation, functionally complements the null allele; expression pattern and physical interactions suggest a possible role in ribosome biogenesis; expression regulated by Gcr1p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.77 0.47 0.46 0.67 0.56 0.65 0.59 0.57 0.64 0.64 0.68 0.69 0.69 0.8 0.92 0.34 0.24 0.18 0.06 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.09 0 0 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0.09 0 0 0 0 0.05 0.05
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.15 0 0.05 0 0.16 0.1 0.09 0.13 0.15 0.14 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0 0 0.11 0.1 0.09 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.37 0.67 0.55 0.47 0.53 0.49 0.38 0.46 0.43 0.41 0.33 0.35 0.28 0.17 0.06 0.64 0.7 0.79 0.84 0.88 0.88 0.87 0.83 0.77
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 1
Bud 0 0 0 0 1 1 1 2 1 1 0 3 0 3 0 0 0 0 0 1 0 1 0 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Cytoplasm 58 49 57 70 101 98 172 177 210 200 93 145 44 127 145 36 34 27 5 4 4 0 2 3
Endoplasmic Reticulum 0 3 1 1 3 0 6 1 8 2 4 1 6 14 6 3 7 3 0 3 1 1 1 5
Endosome 0 2 1 1 1 1 0 1 1 2 0 2 0 1 0 5 9 14 1 5 5 1 5 6
Golgi 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1 3 2 0 0 0 0 1 2
Mitochondria 3 0 19 3 9 6 45 30 29 42 21 29 0 0 0 2 1 2 2 0 1 1 2 4
Nucleus 3 3 4 3 2 4 13 10 4 8 0 4 6 2 2 12 14 14 2 0 2 1 2 1
Nuclear Periphery 0 0 0 1 1 0 2 0 1 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 1 0 0 1 0 0 0 0 0 1 0 0 2 3 0 0 0 1 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 1 0
Vac/Vac Membrane 28 70 69 49 94 74 111 143 141 129 45 74 18 27 9 67 100 121 76 115 126 72 97 111
Unique Cell Count 75 104 125 105 179 151 290 308 326 314 137 210 64 159 157 105 143 153 92 131 145 84 117 145
Labelled Cell Count 92 127 151 128 213 184 350 365 396 385 163 260 74 176 163 127 171 186 92 131 145 84 117 145


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.1 7.5 6.0 6.3 6.0 6.0 5.2 5.4 5.6 5.5 5.8 5.9 7.5 6.1 5.9 8.9 9.4 9.4 7.9 7.9 8.3
Std Deviation (1e-4) 1.4 1.6 1.6 1.6 1.5 1.8 1.8 1.7 1.7 1.6 1.9 1.8 1.5 1.2 0.9 2.3 2.3 2.5 1.7 1.8 1.7
Intensity Change (Log2) – – – 0.07 -0.0 0.01 -0.2 -0.15 -0.1 -0.12 -0.06 -0.03 0.32 0.03 -0.02 0.57 0.64 0.65 0.4 0.39 0.46


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3.2 1.9 3.2 2.6 2.2 3.6 3.5 3.6 4.2 3.0 6.0 8.7 -1.7 -3.8 -5.0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 3.0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3.1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -3.2 -3.0 -3.2 0.1 -1.6 -1.9 -0.5 0 -0.4 -3.3 -5.1 -5.1 -3.5 -4.5 -4.4
Nucleus 0 0 0 0.6 0 0 0 0 0 0 0 0 2.4 2.2 2.0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -1.3 -0.5 -1.0 -3.2 -1.7 -2.3 -2.7 -3.6 -3.6 -3.5 -6.8 -9.2 1.3 2.5 4.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.4236 3.2638 2.881 2.6646 2.9032 2.7406 3.2764 5.7492 5.2072 5.1214 5.0644 5.1834 0.9196 3.2954 2.7733 2.938 2.7331 2.7501
Actin 0.0182 0.0001 0.0175 0 0.0026 0.0035 0.0075 0 0.001 0 0.0084 0.0001 0.0032 0 0.0156 0 0.0037 0.0001
Bud 0.0005 0.0012 0.0018 0.0003 0.003 0.0009 0.0006 0.0006 0.0004 0.0005 0.0003 0.0004 0.0005 0.0003 0.0005 0.0002 0.0006 0.0005
Bud Neck 0.0016 0.0026 0.0011 0.0007 0.0017 0.0023 0.0029 0.001 0.0027 0.0007 0.0012 0.0018 0.0012 0.0015 0.0013 0.0007 0.0021 0.0015
Bud Periphery 0.0007 0.0033 0.0026 0.0008 0.0067 0.0018 0.0014 0.0012 0.001 0.0008 0.0006 0.0013 0.001 0.0007 0.0009 0.0003 0.0012 0.0018
Bud Site 0.0058 0.0043 0.0109 0.0007 0.0021 0.0011 0.0031 0.0047 0.0033 0.0009 0.0013 0.0008 0.0138 0.0018 0.0016 0.0006 0.002 0.0006
Cell Periphery 0.0012 0.0032 0.0028 0.0013 0.0047 0.002 0.0023 0.0018 0.0019 0.0007 0.0006 0.0028 0.0032 0.0022 0.0033 0.0022 0.0012 0.0016
Cytoplasm 0.0578 0.0521 0.0316 0.0072 0.013 0.024 0.0182 0.0251 0.0474 0.0076 0.0088 0.0116 0.0209 0.0275 0.0291 0.0061 0.0064 0.0118
Cytoplasmic Foci 0.0248 0.0112 0.0248 0.0051 0.0114 0.0185 0.0176 0.0114 0.0159 0.008 0.0108 0.005 0.0118 0.006 0.02 0.0121 0.029 0.0034
Eisosomes 0.0003 0 0.0002 0 0.0001 0 0.0002 0 0.0001 0 0.0002 0 0.0001 0 0.0004 0 0 0
Endoplasmic Reticulum 0.0237 0.017 0.0305 0.0077 0.0072 0.0133 0.0176 0.0246 0.0334 0.004 0.0064 0.0148 0.0263 0.0416 0.0291 0.0024 0.0095 0.0151
Endosome 0.2906 0.2151 0.3371 0.1849 0.2568 0.1686 0.2889 0.199 0.2991 0.2227 0.2283 0.1644 0.3106 0.1754 0.3236 0.319 0.191 0.1675
Golgi 0.0108 0.0104 0.0136 0.0024 0.0285 0.0053 0.0147 0.0048 0.0075 0.0031 0.0105 0.0052 0.0085 0.0031 0.019 0.0058 0.0088 0.0046
Lipid Particles 0.017 0.0068 0.0138 0.0019 0.0057 0.0039 0.0148 0.0126 0.0063 0.0015 0.0282 0.0057 0.0159 0.0054 0.0221 0.0205 0.0157 0.0109
Mitochondria 0.0216 0.0028 0.0145 0.0012 0.0161 0.005 0.0204 0.0019 0.0139 0.0025 0.0106 0.0047 0.0132 0.0033 0.0122 0.0007 0.0282 0.0032
None 0.0186 0.0144 0.0252 0.0326 0.0087 0.0205 0.0099 0.0006 0.0044 0.0005 0.0035 0.0035 0.0021 0.0011 0.0099 0.0001 0.0002 0.0002
Nuclear Periphery 0.0286 0.013 0.0139 0.0063 0.0154 0.0079 0.0081 0.0157 0.0164 0.0049 0.0222 0.0158 0.0103 0.0206 0.0162 0.0023 0.0083 0.0177
Nucleolus 0.0016 0.0029 0.0027 0.0013 0.0023 0.0021 0.0021 0.0006 0.001 0.0008 0.0015 0.0011 0.0017 0.0009 0.0012 0.0014 0.0017 0.0019
Nucleus 0.0138 0.0111 0.0048 0.0034 0.0095 0.0056 0.0037 0.0047 0.0051 0.0038 0.0036 0.0054 0.0098 0.0069 0.0029 0.0019 0.0026 0.0069
Peroxisomes 0.0095 0.0003 0.0083 0.0002 0.001 0.0028 0.0033 0.0003 0.0004 0.0002 0.0052 0.0006 0.0005 0.0001 0.0074 0.0002 0.0244 0.0005
Punctate Nuclear 0.0011 0.0003 0.001 0.0005 0.0071 0.0009 0.0008 0.0002 0.0025 0.0001 0.01 0.0004 0.0003 0.0002 0.0013 0.0001 0.0005 0.0001
Vacuole 0.3985 0.5686 0.3847 0.6551 0.5404 0.6302 0.4904 0.6039 0.4684 0.6255 0.5862 0.667 0.4739 0.6233 0.4121 0.5659 0.582 0.6447
Vacuole Periphery 0.0538 0.0592 0.0568 0.0865 0.0562 0.0797 0.0717 0.085 0.0679 0.111 0.0519 0.0875 0.0711 0.0781 0.0703 0.0576 0.081 0.1055

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 120.419 120.5181 92.0344 87.2542 117.9449 81.107 97.6029 83.8414 64.6048 103.7554
Translational Efficiency 1.0901 1.2018 0.9326 1.0508 1.0594 1.4763 0.9886 0.9418 1.114 0.8948

Hit Data

Dataset Hit
Protein Abundance ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘