Standard name
Human Ortholog
Description Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud – – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck – – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site – – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery – – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm – – 0.75 0.88 0.86 0.84 0.82 – 0.79 0.8 0.71 0.76 0.53 0.59 0.6 0.18 0.16 0.08 0.41 0.18 0.57 0.36 0.34 0.35
Endoplasmic Reticulum – – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome – – 0 0 0 0 0 – 0 0 0 0 0.09 0.15 0.11 0 0 0 0 0 0 0 0 0
Golgi – – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0
Mitochondria – – 0 0 0 0.13 0 – 0.17 0.12 0.27 0.19 0 0 0 0 0.05 0.1 0.06 0.08 0.06 0 0 0
Nucleus – – 0.25 0.05 0.06 0 0.07 – 0.05 0 0.05 0.05 0.28 0.17 0.19 0.67 0.76 0.69 0.13 0.37 0.16 0.24 0.25 0.2
Nuclear Periphery – – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus – – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.08 0.06 0 0 0 0.07 0.06
Peroxisomes – – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole – – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.09 0.1
Vac/Vac Membrane – – 0 0.05 0.07 0 0.06 – 0 0.05 0 0.05 0.12 0.14 0.17 0.1 0.05 0.1 0.14 0.19 0.08 0.14 0.11 0.12
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 1
Bud – – 0 0 0 3 0 – 5 2 11 3 0 0 0 0 0 0 0 0 0 4 11 22
Bud Neck – – 0 2 8 1 15 – 7 12 11 5 1 0 0 0 0 0 0 0 0 1 4 8
Bud Site – – 0 0 0 2 0 – 2 1 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery – – 0 0 1 2 4 – 2 1 3 3 0 0 0 0 0 0 0 0 0 0 0 1
Cytoplasm – – 12 151 305 284 533 – 311 323 316 391 96 269 290 24 13 4 9 5 33 72 163 180
Endoplasmic Reticulum – – 0 0 2 0 1 – 0 1 0 1 0 0 1 4 0 2 0 0 1 1 4 7
Endosome – – 0 0 9 6 23 – 8 6 4 11 17 68 54 5 0 1 0 0 0 8 11 14
Golgi – – 0 2 1 0 2 – 0 2 1 0 0 1 0 1 0 0 0 0 0 0 0 1
Mitochondria – – 0 3 1 45 22 – 66 49 120 99 0 0 1 5 4 5 1 2 3 6 20 19
Nucleus – – 4 8 21 14 46 – 21 18 24 25 51 77 91 90 63 36 2 11 9 47 119 99
Nuclear Periphery – – 0 0 0 0 1 – 1 0 0 0 0 1 0 0 0 1 0 0 0 0 1 1
Nucleolus – – 0 0 0 0 0 – 0 1 0 2 1 5 1 4 1 4 1 0 2 8 33 28
Peroxisomes – – 0 0 1 0 0 – 0 1 3 1 0 1 0 1 0 0 0 0 0 0 0 1
SpindlePole – – 0 1 7 1 15 – 4 8 7 7 3 6 11 2 1 1 0 1 1 11 43 52
Vac/Vac Membrane – – 0 8 24 5 38 – 17 19 18 26 21 66 82 13 4 5 3 5 4 27 51 58
Unique Cell Count 16 171 354 339 652 396 405 446 516 181 456 482 134 83 52 22 30 59 200 478 510
Labelled Cell Count 16 175 380 363 700 444 444 518 574 190 494 531 149 86 59 22 30 59 200 478 510


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – – 4.4 4.5 5.3 3.7 4.4 – 3.8 4.2 3.8 4.0 5.2 5.6 5.6 7.7 7.7 7.6 4.6 4.8 4.8
Std Deviation (1e-4) – – 0.6 1.3 1.6 1.2 1.3 – 1.0 1.5 1.3 1.1 1.1 1.7 1.7 2.0 1.7 1.5 1.5 1.7 1.5
Intensity Change (Log2) – – – 0.02 0.26 -0.28 -0.01 – -0.24 -0.09 -0.21 -0.16 0.24 0.33 0.33 0.79 0.79 0.77 0.07 0.12 0.11


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.5 1.2 0.9 0.7 – 0.3 0.5 -0.4 0.1 -1.7 -1.3 -1.2 0 0 0
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 3.3 4.0 3.1
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.4195 -0.9396 -1.0894 -0.8176 -1.5468 -1.367 -0.5456 -0.3709 -0.1755 -0.5257 -0.5366 -0.2004 0.6196 1.2949 0.9221 1.2254 0.6284 0.8627
Actin 0.0808 0.001 0.011 0.0001 0.0007 0.0068 0.0575 0.0024 0.0026 0.0013 0 0.0204 0.0279 0.0001 0.0005 0.0003 0.0005 0.0018
Bud 0.0013 0.0023 0.0003 0 0.0001 0.0004 0.0005 0.0008 0.0056 0.0026 0.0001 0.0002 0.0004 0.0002 0.0007 0.0001 0.0001 0.0003
Bud Neck 0.0053 0.0006 0.0011 0.0007 0.0026 0.0036 0.001 0.0015 0.0015 0.005 0.0005 0.0053 0.0029 0.003 0.0016 0.0006 0.0053 0.0053
Bud Periphery 0.0021 0.0014 0.0005 0 0.0001 0.0006 0.0011 0.0009 0.0064 0.0018 0.0001 0.0003 0.0005 0.0002 0.0006 0 0.0002 0.0004
Bud Site 0.004 0.0153 0.0031 0.0001 0.0003 0.0005 0.0022 0.0063 0.0077 0.0021 0.0002 0.0003 0.0024 0.0014 0.0105 0.0001 0.0006 0.0008
Cell Periphery 0.0004 0.0003 0.0004 0 0 0.0001 0.0005 0.0002 0.0005 0.0006 0 0.0001 0.0003 0.0001 0.0001 0 0.0001 0.0001
Cytoplasm 0.0634 0.048 0.053 0.0129 0.1048 0.095 0.0411 0.0749 0.0405 0.0552 0.0134 0.0958 0.0891 0.062 0.0655 0.0994 0.0437 0.0802
Cytoplasmic Foci 0.0279 0.0049 0.0069 0.0003 0.0432 0.0167 0.0204 0.0176 0.0302 0.0298 0.0437 0.008 0.0104 0.0038 0.0109 0.0052 0.0033 0.0049
Eisosomes 0.0014 0.0001 0.0003 0 0.0001 0.0001 0.0006 0.0001 0.0001 0.0001 0 0.0001 0.0005 0.0001 0.0001 0 0 0.0001
Endoplasmic Reticulum 0.0114 0.0086 0.0027 0.0002 0.0018 0.0024 0.0162 0.0033 0.0034 0.0014 0.0001 0.0011 0.0053 0.0028 0.0015 0.0008 0.0012 0.0009
Endosome 0.0429 0.0064 0.0091 0.0004 0.0182 0.0176 0.0209 0.0134 0.0124 0.0094 0.0016 0.004 0.0203 0.0062 0.006 0.0015 0.0017 0.0046
Golgi 0.0105 0.0002 0.0025 0 0.0064 0.0079 0.0092 0.0036 0.0039 0.0025 0.0002 0.0079 0.0042 0.0001 0.0005 0.0001 0.0001 0.0018
Lipid Particles 0.0158 0.0009 0.0033 0 0.0244 0.0027 0.0166 0.0035 0.0071 0.009 0.0005 0.0024 0.0141 0.0015 0.003 0.0001 0.0002 0.006
Mitochondria 0.0085 0.0004 0.0042 0.0003 0.0013 0.0207 0.0179 0.0011 0.003 0.0108 0.0009 0.0016 0.0024 0.0004 0.0008 0.0003 0.0005 0.0031
None 0.1818 0.2007 0.2143 0.1143 0.0868 0.2103 0.1496 0.252 0.2076 0.219 0.2716 0.147 0.2807 0.3218 0.3544 0.1968 0.3679 0.267
Nuclear Periphery 0.0275 0.0382 0.0221 0.0044 0.0215 0.0217 0.0216 0.0125 0.0107 0.0176 0.0005 0.0073 0.013 0.0037 0.0087 0.0024 0.0062 0.0045
Nucleolus 0.0164 0.0169 0.0145 0.0067 0.0238 0.021 0.0105 0.0173 0.0127 0.0236 0.0633 0.0417 0.0109 0.0287 0.01 0.0062 0.0434 0.0285
Nucleus 0.4133 0.6203 0.5736 0.7959 0.5154 0.5021 0.5588 0.533 0.5737 0.5287 0.575 0.5935 0.4575 0.543 0.4643 0.6719 0.3662 0.5273
Peroxisomes 0.0137 0.0001 0.0009 0 0.0087 0.0022 0.0141 0.0054 0.0165 0.006 0.0009 0.0084 0.0052 0.0001 0.0023 0.0001 0.0001 0.0007
Punctate Nuclear 0.0638 0.0295 0.0725 0.0635 0.1379 0.0644 0.0349 0.0449 0.0516 0.0685 0.0269 0.0539 0.0481 0.0169 0.057 0.0134 0.1552 0.0601
Vacuole 0.0056 0.0032 0.003 0.0002 0.0013 0.0022 0.0026 0.0045 0.0017 0.0038 0.0004 0.0006 0.0031 0.0036 0.001 0.0004 0.0032 0.0012
Vacuole Periphery 0.0023 0.0006 0.0007 0 0.0005 0.001 0.0021 0.0005 0.0006 0.0011 0.0001 0.0002 0.0008 0.0002 0.0002 0.0001 0.0003 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8.2099 16.146 15.2486 16.3743 10.6444 9.8464 15.2122 23.5394 12.2874 18.5066
Translational Efficiency 2.3659 1.1135 1.188 1.0672 1.7742 1.9194 1.2618 0.9939 2.1569 1.288

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
800 692 880 2448 249 407 2379 2212 1049 1099 3259 4660

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 630.02 687.16 813.59 735.68 658.52 761.14 787.47 803.40 636.79 714.56 794.52 767.83
Standard Deviation 72.44 114.57 116.75 88.49 92.96 130.65 106.17 96.38 78.74 125.94 109.74 98.32
Intensity Change Log 2 — 0.125248 0.368904 0.223681 — 0.208935 0.257998 0.286891 — 0.168623 0.313290 0.256331

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000198 0.000734 0.000810 0.000124 0.000813 0.004407 0.000283 0.000381 0.000344 0.002094 0.000426 0.000246
Bud Neck 0.005076 0.020678 0.023221 0.006915 0.005923 0.053745 0.012615 0.014913 0.005277 0.032924 0.015479 0.010712
Bud Site 0.001629 0.010771 0.011304 0.007248 0.007499 0.070199 0.003519 0.004332 0.003022 0.032779 0.005621 0.005864
Cell Periphery 0.000266 0.000362 0.000187 0.000105 0.000288 0.000548 0.000146 0.000117 0.000271 0.000431 0.000157 0.000111
Cytoplasm 0.300647* 0.223865* 0.165500* 0.407095* 0.196112* 0.120607* 0.212912* 0.086934 0.275834* 0.185625* 0.200110* 0.255122*
Cytoplasmic Foci 0.106960 0.042459 0.006547 0.007222 0.066374 0.045686 0.003591 0.001857 0.097326 0.043654 0.004389 0.004675
Eisosomes 0.000114 0.000072 0.000036 0.000018 0.000109 0.000118 0.000018 0.000023 0.000113 0.000089 0.000023 0.000020
Endoplasmic Reticulum 0.001730 0.006226 0.004523 0.001360 0.002761 0.006168 0.001928 0.003692 0.001975 0.006204 0.002628 0.002467
Endosome 0.003721 0.005052 0.001855 0.001169 0.005489 0.033139 0.000879 0.001138 0.004141 0.015453 0.001142 0.001154
Golgi 0.000499 0.000363 0.000139 0.000165 0.002606 0.010899 0.000099 0.000106 0.000999 0.004265 0.000110 0.000137
Lipid Particles 0.008778 0.004681 0.000943 0.000498 0.007894 0.008091 0.000425 0.000675 0.008568 0.005944 0.000565 0.000582
Mitochondria 0.001503 0.005342 0.001744 0.000932 0.006252 0.043439 0.001085 0.001732 0.002630 0.019451 0.001263 0.001312
Mitotic Spindle 0.001941 0.015644 0.027963 0.005878 0.015193 0.088267 0.021196 0.006973 0.005087 0.042539 0.023023 0.006398
None 0.009790 0.016199 0.006864 0.005684 0.010183 0.004744 0.008969 0.005777 0.009883 0.011957 0.008401 0.005728
Nuclear Periphery 0.001343 0.003138 0.001995 0.001433 0.001632 0.002407 0.001827 0.002386 0.001411 0.002868 0.001873 0.001885
Nuclear Periphery Foci 0.001027 0.002285 0.000676 0.000473 0.001133 0.001724 0.000565 0.000520 0.001052 0.002077 0.000595 0.000495
Nucleolus 0.004209 0.011385 0.007033 0.001149 0.009389 0.010032 0.004567 0.009032 0.005439 0.010884 0.005233 0.004891
Nucleus 0.530769* 0.601249* 0.719072* 0.529383* 0.630912* 0.386313* 0.718869* 0.851335* 0.554540* 0.521650* 0.718924* 0.682206*
Peroxisomes 0.002944 0.002701 0.000398 0.000147 0.008177 0.025851 0.000131 0.000278 0.004187 0.011274 0.000203 0.000209
Vacuole 0.016501 0.025597 0.018858 0.022372 0.020545 0.074944 0.006219 0.007434 0.017461 0.043872 0.009632 0.015281
Vacuole Periphery 0.000356 0.001198 0.000331 0.000632 0.000716 0.008673 0.000157 0.000365 0.000441 0.003966 0.000204 0.000505

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -6.87 -7.43 1.18 8.50 8.37 -2.42 1.30 0.94 2.75 -3.90 -3.45 -2.67 0.38 3.58 7.30
Bud Neck -7.52 -9.86 -1.93 6.64 8.96 -7.70 -4.89 -5.07 6.16 -0.31 -10.75 -12.94 -7.17 8.35 7.49
Bud Site -4.92 -5.41 -4.87 2.16 2.31 -6.97 1.19 1.05 7.81 -0.49 -8.54 -3.20 -2.65 7.86 0.54
Cell Periphery -1.86 3.53 9.22 7.52 6.16 -2.62 3.62 4.40 5.94 3.42 -3.37 6.95 10.23 9.38 6.42
Cytoplasm 6.72 14.53 -6.80 -14.83 -27.79 3.78 0.45 7.50 4.32 17.79 8.81 11.37 4.00 -7.28 -12.01
Cytoplasmic Foci 10.16 18.39 18.02 8.17 -2.86 2.62 8.58 8.75 10.12 3.07 10.51 20.44 20.18 12.52 -2.92
Eisosomes 5.86 10.61 15.55 13.58 9.80 -0.52 10.86 10.23 11.21 -4.03 3.78 17.15 18.16 16.86 4.01
Endoplasmic Reticulum -9.46 -9.95 1.23 10.68 11.51 -4.85 1.09 -2.21 4.19 -8.84 -10.84 -5.74 -3.05 10.17 4.33
Endosome -1.43 4.88 7.17 6.97 3.72 -6.98 4.02 3.81 8.91 -2.20 -7.20 7.78 7.85 10.05 0.44
Golgi 0.90 2.52 2.64 3.05 0.87 -2.97 1.47 1.48 5.19 0.42 -3.58 2.15 2.18 5.33 0.42
Lipid Particles 4.96 11.55 12.21 6.79 3.46 0.26 7.30 7.04 3.67 -4.15 3.17 14.15 14.03 6.38 -1.33
Mitochondria -2.23 -2.03 2.03 2.66 5.59 -4.36 1.50 1.31 5.42 -4.11 -5.23 1.32 1.55 5.88 1.55
Mitotic Spindle -4.79 -7.04 -2.55 3.67 6.28 -5.85 -1.93 1.15 7.27 6.49 -7.97 -8.80 -1.21 7.88 9.35
None -1.72 1.93 2.77 4.00 2.29 2.29 0.34 1.88 -1.45 6.73 -0.52 1.06 3.27 3.62 7.46
Nuclear Periphery -7.42 -7.25 -2.87 6.50 5.87 -3.31 -1.98 -3.67 0.86 -2.73 -8.37 -7.81 -6.42 5.16 1.73
Nuclear Periphery Foci -1.61 0.48 1.68 3.84 2.35 -1.95 1.17 0.99 2.64 -0.37 -2.14 1.18 1.67 4.57 0.87
Nucleolus -5.01 -4.35 4.33 6.73 7.26 -0.32 1.28 -0.13 0.30 -2.79 -4.92 -2.54 0.04 5.25 3.11
Nucleus -4.96 -13.65 -1.86 4.40 16.47 8.00 -4.71 -10.23 -22.68 -16.21 0.91 -15.54 -12.23 -12.90 6.31
Peroxisomes 0.52 3.92 4.38 5.56 3.64 -3.35 3.06 3.00 6.19 -4.25 -3.62 5.07 5.08 7.07 0.44
Vacuole -2.37 -0.88 -3.76 -0.38 -2.60 -7.03 4.57 4.02 9.98 -1.85 -7.26 4.87 0.61 8.31 -7.22
Vacuole Periphery -3.12 0.99 -0.64 1.82 -0.86 -4.65 2.52 1.71 4.95 -3.34 -5.32 4.13 0.19 5.29 -1.87
Standard name
Human Ortholog
Description Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation
Localization
Cell Percentages nucleus (70%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Med8

Med8


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Med8-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available