Standard name
Human Ortholog
Description Subunit of chromatin assembly factor I (CAF-1); chromatin assembly by CAF-1 affects multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of DNA damage checkpoint after DNA repair; chromatin dynamics during transcription; and repression of divergent noncoding transcription; Msi1p localizes to nucleus and cytoplasm and independently regulates the RAS/cAMP pathway via sequestration of Npr1p kinase
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.88 0.89 0.97 0.93 0.96 0.85 0.75 0.71 0.71 0.57 0.45 0.5 0.97 0.98 0.97 0.42 0.4 0.42 0.58 0.77 0.68 0.89 0.78 0.81
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0.06 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.06 0.08 0.22 0.39 0.35 0.34 0.58 0.66 0.59 0 0 0 0.06 0 0.07 0.12 0 0.12 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0.07 0.09 0.07 0 0 0 0.18 0.18 0.2 0 0.05 0 0 0 0
Nuclear Periphery 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.05 0 0 0 0.05 0 0 0 0 0.05 0.07 0 0 0 0.4 0.38 0.35 0.09 0.06 0.09 0 0.07 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 1 5 0 1 1
Bud 0 0 1 0 2 3 16 12 12 23 17 18 0 0 0 0 0 1 3 1 2 1 4 6
Bud Neck 0 0 1 0 0 1 3 2 5 3 1 4 0 1 2 0 0 0 0 0 1 0 1 2
Bud Site 0 0 0 1 1 1 1 4 1 10 11 3 0 0 0 0 0 0 – – – – – –
Cell Periphery 3 1 1 0 1 0 1 0 0 1 0 2 5 0 0 0 1 0 1 0 3 0 0 0
Cytoplasm 97 164 365 302 351 412 548 525 525 378 225 310 233 462 508 109 74 71 167 148 253 200 238 269
Endoplasmic Reticulum 0 2 1 1 0 0 0 0 1 1 0 0 1 1 1 8 9 10 18 0 3 0 2 1
Endosome 0 0 1 1 3 5 0 5 6 0 0 2 0 1 1 11 10 9 9 2 3 1 4 3
Golgi 0 0 0 0 0 0 0 1 1 1 0 0 0 0 0 3 4 4 0 0 3 0 2 1
Mitochondria 2 6 3 20 29 105 286 256 256 389 330 367 0 0 0 16 7 11 34 7 43 1 11 13
Nucleus 2 7 4 3 1 5 18 28 28 46 43 46 3 4 5 47 34 33 1 8 5 1 5 4
Nuclear Periphery 9 0 1 1 0 0 0 4 0 1 0 1 0 0 0 0 1 0 1 0 0 0 1 0
Nucleolus 0 0 0 1 1 0 0 0 0 3 3 5 0 0 0 2 0 0 0 0 0 0 0 0
Peroxisomes 0 0 1 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 2 4 4 13 20 13 4 8 0 0 1 1 1 1 7 4 5 3 6 8
Vac/Vac Membrane 0 10 7 10 10 22 24 31 20 24 23 42 2 7 11 104 71 59 25 12 35 7 20 12
Unique Cell Count 110 184 377 323 367 484 732 741 743 666 500 625 240 472 523 260 185 169 289 193 376 227 307 334
Labelled Cell Count 113 190 386 340 401 558 901 883 875 893 657 808 244 476 529 301 212 199 289 193 376 227 307 334


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.3 3.8 3.8 3.6 3.6 3.3 3.1 3.1 3.1 2.8 2.7 2.8 4.9 4.9 4.8 5.7 6.0 5.2 5.0 5.2 5.4
Std Deviation (1e-4) 0.8 0.7 1.2 1.7 1.5 1.5 1.3 1.2 1.2 0.7 0.8 0.7 0.8 0.9 1.0 1.6 2.0 1.6 1.4 1.3 1.7
Intensity Change (Log2) – – – -0.1 -0.06 -0.21 -0.29 -0.3 -0.3 -0.43 -0.52 -0.45 0.37 0.35 0.33 0.59 0.65 0.46 0.4 0.44 0.49


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 12.5 11.8 11.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.2974 2.0041 1.7907 1.357 1.2092 1.9093 0.3335 -0.8465 -1.011 -1.1778 -2.0993 -0.8374 -1.5455 -0.9878 -1.0213 -1.3109 -0.0514 -1.1576
Actin 0.0084 0.0002 0.0001 0.0001 0.0001 0.0003 0.1192 0.0009 0.0221 0.008 0.0008 0.0056 0.0111 0.0016 0.0161 0.015 0.0158 0.0011
Bud 0.0003 0.0003 0.0003 0 0.0001 0.0022 0.0015 0.0088 0.0048 0.0005 0 0.0003 0.0021 0.0463 0.0296 0.0074 0.0086 0.0054
Bud Neck 0.0013 0.0002 0.0001 0.0001 0.0001 0.0016 0.0019 0.0002 0.0153 0.0004 0.0005 0.0007 0.0005 0.0002 0.0011 0.0009 0.0005 0.0048
Bud Periphery 0.0003 0.0004 0.0003 0 0.0001 0.0021 0.0026 0.002 0.0015 0.0006 0 0.0005 0.001 0.0142 0.01 0.0056 0.0033 0.0074
Bud Site 0.0083 0.0006 0.0004 0 0 0.0003 0.0157 0.0033 0.0247 0.0009 0 0.0004 0.0014 0.0068 0.013 0.004 0.0033 0.0011
Cell Periphery 0.0002 0.0001 0.0001 0 0 0.0001 0.0011 0.0002 0.0002 0.0001 0 0.0001 0.0002 0.0004 0.0005 0.0007 0.0003 0.0004
Cytoplasm 0.1322 0.1011 0.0882 0.1052 0.1212 0.1866 0.077 0.2646 0.303 0.2689 0.2051 0.3495 0.1204 0.1486 0.1418 0.2072 0.0642 0.2583
Cytoplasmic Foci 0.0085 0.0031 0.0116 0.0023 0.0184 0.014 0.0775 0.0089 0.0522 0.0106 0.0152 0.0285 0.0532 0.0094 0.0186 0.0151 0.0264 0.0277
Eisosomes 0.0001 0.0001 0.0002 0.0001 0 0.0001 0.0017 0.0001 0.0002 0.0001 0 0.0001 0.0002 0.0002 0.0002 0.0002 0.0004 0
Endoplasmic Reticulum 0.0025 0.0015 0.0015 0.0015 0.0019 0.0033 0.0097 0.0026 0.0025 0.003 0.0023 0.0036 0.0053 0.0021 0.0028 0.0015 0.0011 0.0024
Endosome 0.0073 0.0009 0.0045 0.0018 0.044 0.0064 0.0758 0.0087 0.0303 0.0067 0.0021 0.0251 0.0381 0.0041 0.0108 0.005 0.0958 0.012
Golgi 0.0005 0 0.0001 0 0.0006 0.0003 0.0242 0.0019 0.0166 0.0106 0.0001 0.0207 0.0108 0.0005 0.0064 0.0042 0.0196 0.0018
Lipid Particles 0.0013 0.0003 0.0012 0.0001 0.0009 0.0016 0.0687 0.0014 0.0029 0.0064 0.0002 0.0283 0.0127 0.002 0.0137 0.0008 0.0327 0.0036
Mitochondria 0.0003 0.0004 0.0005 0.0004 0.0013 0.0008 0.05 0.0097 0.0107 0.0148 0.0001 0.0227 0.0099 0.0026 0.0122 0.0105 0.1265 0.002
None 0.7653 0.8303 0.8574 0.8395 0.796 0.6725 0.2479 0.637 0.4613 0.583 0.7112 0.4245 0.569 0.6586 0.5742 0.5192 0.4696 0.4953
Nuclear Periphery 0.0027 0.0046 0.0019 0.0104 0.0022 0.0048 0.0323 0.0023 0.0034 0.0046 0.0036 0.0057 0.0192 0.0081 0.0072 0.0054 0.0076 0.0062
Nucleolus 0.0007 0.0009 0.001 0.0006 0.0003 0.0018 0.0033 0.0006 0.0006 0.0008 0.0002 0.0005 0.0024 0.0016 0.0033 0.0046 0.0102 0.0024
Nucleus 0.0377 0.0482 0.0181 0.0259 0.0035 0.0739 0.0931 0.0301 0.0232 0.0682 0.0532 0.0535 0.1193 0.0734 0.1051 0.1084 0.0484 0.1138
Peroxisomes 0.0012 0.0001 0.0003 0.0001 0.0003 0.0006 0.0317 0.0005 0.0039 0.0056 0.0002 0.0127 0.0029 0.0009 0.0073 0.0005 0.0417 0.0033
Punctate Nuclear 0.011 0.0055 0.0076 0.0105 0.0012 0.0227 0.0493 0.0039 0.0117 0.0042 0.0048 0.0106 0.0109 0.0155 0.0233 0.0804 0.0141 0.0348
Vacuole 0.009 0.0011 0.0043 0.001 0.0057 0.0037 0.0115 0.0109 0.0072 0.0015 0.0004 0.0052 0.0065 0.0023 0.0018 0.0024 0.0058 0.0127
Vacuole Periphery 0.0009 0.0002 0.0003 0.0003 0.0021 0.0004 0.0042 0.0014 0.0016 0.0006 0.0001 0.0013 0.0029 0.0006 0.0012 0.0008 0.004 0.0033

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 11.7383 14.7684 4.0375 11.2099 10.1462 8.6903 9.2345 9.8131 10.1063 12.9437
Translational Efficiency 1.1587 0.6308 2.3392 0.7619 1.0229 1.5811 0.9851 0.7215 0.914 0.8824

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
929 842 142 1048 1438 2185 144 429 2367 3027 286 1477

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 640.23 716.96 914.03 770.12 655.42 718.11 887.20 855.18 649.46 717.79 900.52 794.83
Standard Deviation 76.44 84.66 106.80 131.85 82.58 90.20 96.94 104.45 80.57 88.69 102.84 130.36
Intensity Change Log 2 — 0.163302 0.513651 0.266493 — 0.131785 0.436840 0.383808 — 0.147445 0.475306 0.327030

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000179 0.000307 0.001049 0.000841 0.000106 0.000526 0.000239 0.001168 0.000135 0.000465 0.000641 0.000936
Bud Neck 0.008871 0.028434 0.003809 0.015098 0.006879 0.021071 0.001931 0.014851 0.007661 0.023119 0.002864 0.015026
Bud Site 0.003356 0.010471 0.001310 0.040238 0.004814 0.016276 0.000752 0.046489 0.004241 0.014661 0.001029 0.042053
Cell Periphery 0.000170 0.000119 0.000115 0.000318 0.000184 0.000208 0.000158 0.000152 0.000179 0.000183 0.000137 0.000270
Cytoplasm 0.469388* 0.392999* 0.024592 0.335226* 0.507425* 0.473732* 0.111838* 0.358833* 0.492496* 0.451275* 0.068520 0.342083*
Cytoplasmic Foci 0.150950 0.183434* 0.002011 0.011299 0.103623 0.113883 0.015889 0.021465 0.122198 0.133229 0.008999 0.014252
Eisosomes 0.000147 0.000121 0.000069 0.000051 0.000112 0.000099 0.000091 0.000060 0.000126 0.000105 0.000080 0.000053
Endoplasmic Reticulum 0.000448 0.000843 0.010849 0.005759 0.000684 0.000646 0.003455 0.003009 0.000592 0.000700 0.007126 0.004960
Endosome 0.007741 0.019017 0.000940 0.005381 0.005598 0.011655 0.001875 0.013708 0.006439 0.013703 0.001410 0.007800
Golgi 0.000573 0.003137 0.000086 0.003355 0.000813 0.001688 0.000064 0.004165 0.000719 0.002091 0.000075 0.003590
Lipid Particles 0.004913 0.006599 0.001431 0.001541 0.003492 0.004707 0.002592 0.001511 0.004049 0.005234 0.002015 0.001533
Mitochondria 0.002957 0.012073 0.000782 0.003119 0.001941 0.006086 0.000437 0.011455 0.002340 0.007751 0.000609 0.005540
Mitotic Spindle 0.001107 0.007353 0.000216 0.024307 0.002169 0.013381 0.000161 0.015028 0.001752 0.011704 0.000189 0.021612
None 0.003527 0.003588 0.002306 0.009510 0.003873 0.002422 0.008042 0.012609 0.003737 0.002746 0.005194 0.010410
Nuclear Periphery 0.000460 0.000735 0.001284 0.002523 0.000516 0.000625 0.001134 0.001341 0.000494 0.000656 0.001209 0.002179
Nuclear Periphery Foci 0.000376 0.000382 0.000665 0.002262 0.000510 0.000211 0.000473 0.000999 0.000457 0.000258 0.000569 0.001895
Nucleolus 0.000853 0.001841 0.003672 0.001532 0.000733 0.001144 0.002522 0.000526 0.000780 0.001338 0.003093 0.001240
Nucleus 0.256167* 0.203571* 0.923034* 0.456904* 0.292245* 0.241372* 0.808889* 0.336042* 0.278085* 0.230857* 0.865562* 0.421799*
Peroxisomes 0.002157 0.018442 0.000366 0.000656 0.001449 0.005497 0.000403 0.001511 0.001727 0.009098 0.000385 0.000904
Vacuole 0.085204 0.104931 0.021252 0.078443 0.062210 0.082958 0.038924 0.153608 0.071234 0.089070 0.030150 0.100275
Vacuole Periphery 0.000456 0.001604 0.000161 0.001639 0.000625 0.001812 0.000130 0.001467 0.000558 0.001754 0.000146 0.001589

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.24 -6.56 -4.25 -3.98 2.99 -2.15 -4.14 -4.12 -0.89 -2.17 -2.28 -7.58 -6.37 -1.91 0.84
Bud Neck -6.13 1.79 -2.66 4.31 -4.47 -9.70 4.95 -0.82 6.11 -3.20 -11.16 3.98 -3.49 6.43 -6.15
Bud Site -4.31 0.81 -6.28 -3.75 -6.66 -5.89 3.03 -3.34 -0.86 -4.24 -7.30 2.85 -6.81 -2.64 -8.04
Cell Periphery 4.88 1.06 -2.48 -5.23 -2.85 -0.50 1.25 3.34 1.53 1.27 -0.05 1.72 -1.35 -0.60 -2.44
Cytoplasm 5.90 40.96 18.71 11.21 -21.74 3.73 24.28 18.84 17.05 -7.23 5.56 42.06 28.45 24.23 -16.92
Cytoplasmic Foci -3.49 29.11 27.07 26.00 -7.53 -2.08 19.48 24.30 28.92 0.62 -2.29 34.19 36.36 39.22 -1.49
Eisosomes 2.53 3.76 8.93 5.66 2.15 2.18 -0.00 3.38 1.42 1.79 3.90 2.46 9.99 6.45 3.31
Endoplasmic Reticulum -3.34 -9.65 -14.46 -13.59 4.18 0.17 -7.05 -12.68 -14.12 -0.08 -0.85 -11.23 -16.99 -17.30 2.94
Endosome -5.59 6.83 4.21 8.72 -4.11 -7.15 5.33 0.55 4.00 -1.42 -8.56 8.94 3.55 10.93 -3.24
Golgi -2.66 2.45 -0.98 1.49 -1.72 -2.34 3.67 -0.16 0.95 -1.25 -3.66 4.38 -0.82 1.36 -2.08
Lipid Particles -1.79 6.23 7.45 5.80 -0.22 -2.40 0.61 4.25 6.59 2.17 -2.61 4.14 8.16 9.04 1.80
Mitochondria -3.27 1.77 0.78 3.94 -2.46 -3.82 2.01 -0.57 1.78 -1.48 -4.86 2.60 0.08 5.05 -2.73
Mitotic Spindle -2.37 -0.88 -6.32 -3.77 -2.54 -5.27 0.87 -3.30 -0.75 -3.57 -5.92 -0.62 -7.04 -3.03 -4.51
None 0.06 0.76 -3.24 -2.72 -3.62 2.44 -1.54 -2.47 -3.91 0.33 2.07 -1.18 -3.98 -5.87 -0.78
Nuclear Periphery -4.98 -7.65 -15.87 -14.13 -7.69 -1.59 -6.73 -8.18 -7.80 -3.78 -3.40 -10.05 -17.56 -16.52 -9.16
Nuclear Periphery Foci 0.35 -1.27 -4.23 -4.89 -3.34 1.85 -1.48 -5.64 -9.05 -3.88 1.74 -1.95 -5.44 -6.53 -4.24
Nucleolus -2.99 -2.41 -0.93 2.24 2.13 -2.99 -1.97 1.59 4.87 2.30 -3.84 -3.16 -1.08 1.99 2.78
Nucleus 4.51 -40.23 -17.82 -21.99 24.37 6.03 -20.69 -4.07 -7.60 15.75 6.95 -38.26 -17.05 -23.00 24.51
Peroxisomes -7.11 4.93 4.75 8.01 -0.58 -5.65 3.04 2.76 7.88 -0.40 -8.97 5.28 4.99 11.11 -0.67
Vacuole -3.24 4.78 -9.21 -6.40 -12.04 -5.04 -1.16 -16.09 -14.74 -11.83 -5.15 1.48 -18.41 -16.02 -14.70
Vacuole Periphery -5.47 2.09 -0.99 2.89 -2.25 -2.92 2.78 0.92 3.42 -1.25 -4.01 3.37 -0.30 3.45 -2.58