Standard name
Human Ortholog
Description Glycolytic enzyme phosphoglucose isomerase; catalyzes the interconversion of glucose-6-phosphate and fructose-6-phosphate; required for cell cycle progression and completion of the gluconeogenic events of sporulation

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0.05 0.07 0.06 0.07 0.07 0 0 0 0 0.06 0.05 0 0 0 0 0 0
Cytoplasm 1.0 1.0 0.99 1.0 0.99 0.98 0.96 0.93 0.89 0.88 0.92 1.0 0.99 0.99 1.0 0.99 0.99 0.98 0.95 0.95 0.91 0.95 0.97
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0.07 0.08 0 0 0 0 0 0.08 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0
Bud 1 0 1 2 2 1 3 3 2 4 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1 4 3 4 7 15 20 27 30 31 36 0 0 1 2 9 9 0 0 0 0 0 0
Cytoplasm 252 151 133 308 367 483 419 378 459 372 469 115 196 221 110 152 184 236 152 123 64 92 178
Endoplasmic Reticulum 0 0 1 0 1 3 5 15 38 35 19 0 1 0 3 13 13 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0
Golgi 0 0 0 0 1 0 0 0 0 0 0 0 0 2 0 1 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 3 0 0 0 1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 1 1 0 2 0 0 0 0
Unique Cell Count 253 151 134 309 371 493 438 408 515 424 509 115 197 223 110 153 186 241 161 130 71 97 184
Labelled Cell Count 254 155 138 314 378 502 447 423 530 442 525 115 197 225 115 176 207 241 161 130 71 97 184


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 288.1 356.8 271.2 274.9 266.9 241.5 227.0 200.4 197.7 182.3 187.9 306.7 331.8 315.3 398.5 402.8 371.9 215.1 265.7 286.8
Std Deviation (1e-4) 46.2 67.9 48.5 59.1 58.2 45.5 50.2 51.9 47.0 48.6 47.2 81.6 71.6 76.1 77.2 98.8 88.1 74.2 84.0 81.9
Intensity Change (Log2) 0.02 -0.02 -0.17 -0.26 -0.44 -0.46 -0.57 -0.53 0.18 0.29 0.22 0.56 0.57 0.46 -0.33 -0.03 0.08


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 1.2 1.9 1.7 2.1 2.1 0 0 0 0 1.5 1.2
Cytoplasm 0.6 -0.3 -1.0 -2.0 -2.9 -3.7 -4.0 -3.0 0.9 0.3 -0.2 0.9 0.1 -0.3
Endoplasmic Reticulum 0 0 0 0 0 2.9 3.1 0 0 0 0 0 3.0 2.7
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 275.518 344.8553 306.8002 288.3701 259.1011 309.2865 262.9257 359.0397 312.3092 329.2102 286.6066 367.8732 247.8109 329.8006 291.6593 278.5254 227.3762 261.3002
Actin 0.0024 0.0002 0.0001 0.0005 0.0004 0.0002 0.0104 0.0001 0.0004 0.0001 0.0047 0.0002 0.0045 0.0001 0.0249 0.0285 0.0616 0.0035
Bud 0.0014 0.0001 0.001 0.0001 0.0015 0.0001 0.0007 0.0001 0.0002 0.0001 0.002 0.0002 0.0026 0 0.0016 0.0008 0.0036 0.0004
Bud Neck 0.0005 0.0002 0.0004 0.0003 0.0006 0.0009 0.0002 0.0001 0.0001 0.0002 0.0006 0.0003 0.0007 0.0001 0.0004 0.0002 0.0029 0.0007
Bud Periphery 0.0008 0 0.0004 0 0.0013 0 0.0005 0 0.0001 0 0.0018 0 0.0015 0 0.0011 0.0008 0.0021 0.0003
Bud Site 0.0015 0 0.0002 0 0.0005 0 0.0003 0 0.0001 0 0.0005 0 0.004 0 0.0005 0.0012 0.0024 0.0012
Cell Periphery 0.0017 0.0004 0.0011 0.0001 0.0038 0.0001 0.0005 0.0002 0.0002 0 0.0008 0 0.0008 0 0.0022 0.0002 0.0005 0.0001
Cytoplasm 0.9397 0.9962 0.9698 0.9472 0.8501 0.9919 0.8822 0.996 0.954 0.9878 0.841 0.9869 0.8641 0.9981 0.9202 0.8415 0.7555 0.8505
Cytoplasmic Foci 0.0044 0.0001 0.0017 0.0039 0.0278 0.0003 0.0031 0.0001 0.0007 0.0001 0.0021 0.0002 0.0232 0 0.0068 0.0265 0.0145 0.0174
Eisosomes 0.0004 0 0 0 0.0004 0 0.0006 0 0.0001 0 0 0 0.0005 0 0.0003 0.0004 0.0005 0.0001
Endoplasmic Reticulum 0.001 0.0002 0.0003 0.0002 0.0007 0.0002 0.0024 0 0.0084 0 0.0026 0.0001 0.0005 0 0.0017 0.0002 0.0016 0.0002
Endosome 0.0018 0 0.0001 0.0056 0.0003 0.0001 0.0023 0 0.0013 0 0.0317 0 0.0093 0 0.0031 0.0022 0.0226 0.0196
Golgi 0.0003 0 0 0.004 0.0001 0 0.0007 0 0.0001 0 0.0109 0 0.0057 0 0.0036 0.0029 0.0202 0.0269
Lipid Particles 0.0016 0 0.0003 0.0108 0.0015 0 0.0009 0 0.0004 0 0.0006 0 0.0143 0 0.0033 0.0042 0.0143 0.0248
Mitochondria 0.0044 0 0.0014 0.0028 0.0152 0.0001 0.0099 0.0001 0.0037 0.0002 0.0617 0.0001 0.0077 0 0.0055 0.0054 0.0537 0.0313
None 0.0051 0.0001 0.007 0.0003 0.0318 0.0002 0.0059 0.0002 0.0009 0.0002 0.0005 0.0003 0.0101 0.0001 0.0044 0.0331 0.0029 0.0003
Nuclear Periphery 0.0053 0.0006 0.0015 0.0025 0.004 0.0012 0.0283 0.0005 0.0105 0.0017 0.0028 0.0015 0.0029 0.0002 0.0011 0.0013 0.0036 0.0013
Nucleolus 0.0055 0 0.0037 0.0012 0.0145 0.0001 0.0057 0 0.0034 0.0001 0.0003 0 0.014 0 0.0068 0.0024 0.0018 0.0003
Nucleus 0.0112 0.0013 0.0043 0.0137 0.0161 0.0041 0.0316 0.0021 0.0104 0.0072 0.0041 0.0099 0.0098 0.0013 0.0032 0.0047 0.0114 0.0038
Peroxisomes 0.0004 0 0.0001 0.0007 0.0009 0 0.0004 0 0.0001 0 0.0003 0 0.0106 0 0.0077 0.016 0.0161 0.0147
Punctate Nuclear 0.0021 0 0.0002 0.0002 0.0019 0.0001 0.0011 0 0.0002 0 0.0001 0.0002 0.0103 0 0.0004 0.0268 0.0027 0.0007
Vacuole 0.006 0.0004 0.0054 0.0031 0.0206 0.0004 0.0057 0.0004 0.0018 0.0009 0.013 0.0001 0.0018 0 0.0006 0.0004 0.0032 0.0011
Vacuole Periphery 0.0024 0.0001 0.0011 0.0025 0.0059 0.0002 0.0065 0.0001 0.003 0.001 0.0181 0.0001 0.0009 0 0.0004 0.0001 0.0023 0.0009

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 1291.9441 1342.4195 1482.7702 1413.1398 1385.5027 1405.4823 1641.8408 1701.413 1769.1779 1573.4582
Translational Efficiency 1.4637 1.5043 1.4838 1.517 1.4478 1.4297 1.6319 1.5104 1.5821 1.5576

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1102 453 1678 380 1674 1946 1468 622 2776 2399 3146 1002

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 8928.74 16500.20 20550.00 29420.11 16460.12 17707.89 24027.07 27786.88 13470.36 17479.84 22172.49 28406.27
Standard Deviation 2615.75 4797.65 6422.83 6837.92 3490.60 4419.82 5392.97 6172.27 4862.30 4518.38 6211.57 6481.45
Intensity Change Log 2 0.885955 1.202610 1.720274 0.105417 0.545686 0.755429 0.430142 0.812106 1.171996

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000203 0.000078 0.000086 0.000191 0.000039 0.000038 0.000076 0.000137 0.000104 0.000046 0.000081 0.000157
Bud Neck 0.000815 0.000547 0.001149 0.001501 0.000314 0.000218 0.000959 0.001245 0.000513 0.000281 0.001060 0.001342
Bud Site 0.008725 0.002179 0.002194 0.007519 0.000984 0.000567 0.001771 0.004436 0.004057 0.000872 0.001997 0.005606
Cell Periphery 0.017162 0.012845 0.011691 0.050037 0.004122 0.003190 0.013304 0.039486 0.009299 0.005013 0.012443 0.043487
Cytoplasm 0.798940* 0.834595* 0.825587* 0.699480* 0.885679* 0.911093* 0.824374* 0.730219* 0.851246* 0.896648* 0.825021* 0.718561*
Cytoplasmic Foci 0.013819 0.008008 0.009129 0.005326 0.006211 0.004753 0.006289 0.006785 0.009231 0.005368 0.007804 0.006232
Eisosomes 0.000003 0.000002 0.000003 0.000021 0.000001 0.000001 0.000002 0.000005 0.000001 0.000001 0.000002 0.000011
Endoplasmic Reticulum 0.000329 0.000030 0.000056 0.000028 0.000045 0.000019 0.000055 0.000024 0.000157 0.000021 0.000056 0.000026
Endosome 0.000446 0.000060 0.000093 0.000050 0.000085 0.000033 0.000108 0.000048 0.000228 0.000038 0.000100 0.000049
Golgi 0.026414 0.006279 0.004215 0.001444 0.008225 0.002711 0.004078 0.002268 0.015446 0.003385 0.004151 0.001955
Lipid Particles 0.007038 0.004311 0.006463 0.021919 0.002543 0.001292 0.007659 0.014934 0.004328 0.001862 0.007021 0.017583
Mitochondria 0.000490 0.000127 0.000157 0.000114 0.000111 0.000144 0.000093 0.000116 0.000261 0.000141 0.000127 0.000115
Mitotic Spindle 0.001550 0.000376 0.000607 0.000173 0.000880 0.000384 0.000590 0.000117 0.001146 0.000383 0.000599 0.000138
None 0.006228 0.010658 0.004965 0.002255 0.005975 0.010517 0.004177 0.004029 0.006075 0.010543 0.004597 0.003356
Nuclear Periphery 0.000382 0.000363 0.000694 0.000388 0.000382 0.000159 0.000532 0.000381 0.000382 0.000197 0.000619 0.000384
Nuclear Periphery Foci 0.004263 0.003057 0.003144 0.002443 0.003817 0.001431 0.003444 0.002787 0.003994 0.001738 0.003284 0.002657
Nucleolus 0.000291 0.000081 0.000057 0.000122 0.000063 0.000069 0.000063 0.000097 0.000153 0.000071 0.000060 0.000106
Nucleus 0.041841 0.075559 0.082493 0.031565 0.052372 0.053002 0.069601 0.057099 0.048191 0.057262 0.076477 0.047416
Peroxisomes 0.000030 0.000016 0.000014 0.000042 0.000007 0.000008 0.000009 0.000025 0.000016 0.000009 0.000012 0.000031
Vacuole 0.029720 0.036664 0.044029 0.172801 0.018610 0.009217 0.059700 0.133059 0.023020 0.014400 0.051342 0.148131
Vacuole Periphery 0.041314 0.004163 0.003173 0.002581 0.009536 0.001155 0.003117 0.002702 0.022151 0.001723 0.003147 0.002656

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 4.11 3.78 0.35 -4.18 -3.84 -0.36 -4.71 -6.35 -6.00 -2.19 4.57 0.46 -3.20 -7.88 -4.20
Bud Neck 1.36 -3.20 -3.92 -5.39 -1.21 1.72 -7.47 -6.50 -6.98 -0.03 3.58 -8.29 -7.45 -9.60 -0.64
Bud Site 4.93 5.09 1.08 -4.07 -4.23 2.55 -4.30 -4.25 -4.78 -2.48 6.29 3.12 -1.68 -6.99 -4.51
Cell Periphery 1.76 2.61 -5.51 -6.32 -6.75 1.68 -8.07 -11.77 -12.11 -8.05 5.07 -3.60 -11.87 -13.84 -10.59
Cytoplasm -2.04 -0.01 6.43 7.55 6.82 -4.74 12.21 15.41 18.30 6.88 -8.20 9.21 15.41 20.71 9.81
Cytoplasmic Foci 3.64 2.18 3.24 0.69 2.02 1.53 -1.84 -0.89 -1.64 0.03 4.88 -0.25 1.42 -1.43 1.61
Eisosomes 1.03 -1.40 -1.44 -1.48 -1.37 -0.30 -7.22 -8.34 -8.15 -4.98 2.56 -6.22 -2.28 -2.40 -1.89
Endoplasmic Reticulum 8.42 7.66 8.56 0.66 5.08 4.22 -0.88 3.20 -1.51 2.88 9.28 6.45 8.97 -1.29 5.30
Endosome 7.93 7.02 8.17 1.22 5.27 3.98 -2.17 2.42 -2.21 4.73 9.01 5.13 8.34 -2.07 6.74
Golgi 9.56 11.26 12.79 5.45 8.95 8.42 6.12 9.39 1.39 3.79 13.19 12.70 15.38 4.04 7.23
Lipid Particles 2.09 0.00 -5.07 -6.21 -5.34 3.22 -8.49 -7.63 -8.44 -4.05 4.91 -5.59 -8.88 -10.90 -6.74
Mitochondria 5.71 5.14 2.71 -0.20 0.25 -1.19 0.25 -0.39 0.69 -0.48 1.97 4.41 2.24 0.43 0.18
Mitotic Spindle 4.98 3.29 6.71 0.79 3.96 1.57 0.33 2.62 2.04 1.64 3.56 1.44 5.20 1.99 3.26
None -2.04 1.37 4.14 4.86 7.42 -5.25 4.50 3.95 7.55 0.46 -5.23 3.49 5.63 9.58 3.85
Nuclear Periphery -0.02 -5.53 -0.52 -0.46 4.35 5.65 -3.52 -0.19 -6.46 3.49 5.91 -6.42 -0.49 -5.79 5.85
Nuclear Periphery Foci 2.04 1.89 4.18 1.35 2.60 5.58 0.32 2.10 -2.77 2.07 6.97 1.40 3.66 -2.70 2.81
Nucleolus 1.30 1.43 1.08 -1.87 -3.26 -0.72 -0.49 -1.70 0.10 -2.24 0.81 1.36 0.81 -0.19 -4.01
Nucleus -6.16 -12.83 1.31 6.78 12.95 -0.30 -7.55 -1.78 -1.59 4.53 -3.76 -14.46 -0.83 2.34 11.44
Peroxisomes 1.86 2.25 -1.12 -3.13 -3.57 -0.75 -1.42 -3.47 -3.11 -2.84 2.25 1.24 -3.10 -5.17 -3.97
Vacuole -2.13 -5.22 -12.93 -11.03 -10.95 4.61 -12.89 -15.80 -17.28 -8.26 4.04 -13.34 -20.32 -21.59 -13.96
Vacuole Periphery 9.99 10.47 10.68 1.70 1.72 6.79 5.07 5.63 -3.94 1.69 12.35 11.44 11.88 -2.32 2.41