Standard name | |
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Human Ortholog | |
Description | Subunit (90 kDa) of TFIID and SAGA complexes; involved in RNA polymerase II transcription initiation and in chromatin modification |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | – | 1 | 3 | 4 | 2 | 6 | 2 |
Bud | 0 | 2 | 0 | 0 | 0 | 0 | 3 | 4 | 2 | 5 | 9 | 4 | 0 | 1 | 0 | 0 | 0 | – | 1 | 0 | 1 | 3 | 4 | 5 |
Bud Neck | 0 | 1 | 5 | 2 | 7 | 5 | 14 | 18 | 2 | 9 | 9 | 12 | 0 | 0 | 0 | 3 | 11 | – | 0 | 1 | 1 | 2 | 2 | 4 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 2 | 3 | 3 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 2 | 4 | – | 1 | 1 | 3 | 3 | 1 | 2 |
Cytoplasm | 124 | 199 | 271 | 199 | 340 | 200 | 316 | 290 | 152 | 139 | 199 | 255 | 161 | 269 | 254 | 492 | 518 | – | 110 | 149 | 164 | 96 | 161 | 182 |
Endoplasmic Reticulum | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 49 | 25 | – | 2 | 0 | 0 | 2 | 1 | 6 |
Endosome | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 4 | – | 4 | 4 | 1 | 2 | 1 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | – | 0 | 0 | 0 | 1 | 2 | 0 |
Mitochondria | 19 | 19 | 1 | 3 | 2 | 9 | 30 | 25 | 46 | 55 | 77 | 39 | 8 | 1 | 0 | 26 | 9 | – | 16 | 21 | 30 | 15 | 34 | 23 |
Nucleus | 0 | 1 | 1 | 2 | 2 | 0 | 2 | 4 | 3 | 3 | 1 | 1 | 2 | 1 | 0 | 3 | 2 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 1 |
SpindlePole | 0 | 0 | 2 | 0 | 1 | 3 | 7 | 4 | 3 | 4 | 9 | 10 | 0 | 0 | 0 | 0 | 0 | – | 0 | 3 | 1 | 1 | 4 | 2 |
Vac/Vac Membrane | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 2 | 0 | 1 | 1 | 3 | 4 | – | 2 | 7 | 4 | 5 | 8 | 18 |
Unique Cell Count | 137 | 213 | 277 | 204 | 345 | 210 | 333 | 306 | 178 | 179 | 250 | 285 | 163 | 271 | 256 | 502 | 530 | 147 | 203 | 234 | 142 | 239 | 257 | |
Labelled Cell Count | 144 | 224 | 284 | 210 | 355 | 218 | 372 | 350 | 209 | 215 | 307 | 325 | 172 | 275 | 257 | 581 | 582 | 147 | 203 | 234 | 142 | 239 | 257 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.3 | 2.9 | 4.2 | 3.9 | 4.6 | 3.5 | 3.4 | 3.8 | 3.2 | 2.9 | 2.7 | 3.5 | 3.6 | 3.8 | 4.1 | 5.1 | 5.1 | 4.8 | 3.5 | 3.5 | 4.1 |
Std Deviation (1e-4) | 0.4 | 0.5 | 0.9 | 1.4 | 1.8 | 1.2 | 1.0 | 1.1 | 1.4 | 0.7 | 0.6 | 1.6 | 0.7 | 1.3 | 1.7 | 1.2 | 1.0 | 0.8 | 1.4 | 1.3 | 2.0 |
Intensity Change (Log2) | – | – | – | -0.1 | 0.14 | -0.28 | -0.31 | -0.14 | -0.41 | -0.54 | -0.62 | -0.26 | -0.21 | -0.13 | -0.04 | 0.29 | 0.27 | 0.2 | -0.25 | -0.28 | -0.02 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.422 | 1.3575 | 1.1996 | 0.9831 | 1.5335 | 1.3046 | -0.9791 | -1.2025 | -1.2583 | -1.3054 | -0.4186 | -1.3569 | -1.8873 | -1.5584 | -1.4515 | -1.144 | -0.4259 | -1.5389 |
Actin | 0.0279 | 0.0002 | 0.0022 | 0.0022 | 0.0593 | 0.0015 | 0.0302 | 0.0014 | 0.0065 | 0.0005 | 0.0119 | 0.0114 | 0.0468 | 0 | 0.0108 | 0 | 0.1196 | 0.006 |
Bud | 0.0006 | 0.0006 | 0.0005 | 0.0009 | 0.0007 | 0.0004 | 0.0013 | 0.0061 | 0.0004 | 0.0002 | 0.0052 | 0.0008 | 0.0003 | 0 | 0.0001 | 0 | 0.0015 | 0.0004 |
Bud Neck | 0.0011 | 0.0002 | 0.0006 | 0.0002 | 0.0013 | 0.0005 | 0.0007 | 0.001 | 0.0003 | 0.0017 | 0.0032 | 0.0003 | 0.0003 | 0 | 0.0002 | 0 | 0.0072 | 0.0015 |
Bud Periphery | 0.0017 | 0.0015 | 0.0017 | 0.0033 | 0.0016 | 0.0014 | 0.0032 | 0.0082 | 0.0007 | 0.0001 | 0.0052 | 0.0049 | 0.0004 | 0 | 0.0003 | 0 | 0.0031 | 0.0009 |
Bud Site | 0.0038 | 0.0025 | 0.0052 | 0.0004 | 0.0022 | 0.0003 | 0.0029 | 0.027 | 0.0015 | 0.002 | 0.0362 | 0.0003 | 0.0014 | 0 | 0.0005 | 0 | 0.0044 | 0.0012 |
Cell Periphery | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0005 | 0.0008 | 0.0001 | 0.0001 | 0.0005 | 0.0002 | 0.0003 | 0 | 0.0001 | 0 | 0.0004 | 0.0001 |
Cytoplasm | 0.0219 | 0.0162 | 0.0222 | 0.0248 | 0.0277 | 0.0347 | 0.0302 | 0.0286 | 0.0378 | 0.0409 | 0.0325 | 0.0521 | 0.0082 | 0.0325 | 0.0409 | 0.0072 | 0.029 | 0.0766 |
Cytoplasmic Foci | 0.0171 | 0.0022 | 0.013 | 0.0065 | 0.0344 | 0.0073 | 0.0312 | 0.0182 | 0.0168 | 0.0217 | 0.0213 | 0.0141 | 0.0077 | 0.0015 | 0.0045 | 0.0008 | 0.0058 | 0.005 |
Eisosomes | 0.0005 | 0.0002 | 0.0002 | 0.0001 | 0.001 | 0.0001 | 0.0006 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.0002 | 0.0016 | 0.0002 | 0.0003 | 0.0001 | 0.0008 | 0.0001 |
Endoplasmic Reticulum | 0.0021 | 0.0002 | 0.0009 | 0.0002 | 0.0032 | 0.001 | 0.0031 | 0.0004 | 0.0017 | 0.0015 | 0.0023 | 0.0013 | 0.0017 | 0.0006 | 0.0044 | 0.0001 | 0.0114 | 0.0008 |
Endosome | 0.0294 | 0.002 | 0.0069 | 0.024 | 0.0261 | 0.0029 | 0.038 | 0.0047 | 0.0171 | 0.0237 | 0.0624 | 0.0071 | 0.0071 | 0.0002 | 0.0034 | 0 | 0.0209 | 0.0023 |
Golgi | 0.0089 | 0.0004 | 0.0038 | 0.0315 | 0.0134 | 0.0013 | 0.0168 | 0.0004 | 0.005 | 0.0127 | 0.0222 | 0.0022 | 0.0035 | 0 | 0.0048 | 0 | 0.0105 | 0.0009 |
Lipid Particles | 0.0249 | 0.0018 | 0.0034 | 0.0026 | 0.0455 | 0.0048 | 0.0183 | 0.0014 | 0.0053 | 0.0144 | 0.0499 | 0.003 | 0.0038 | 0.0002 | 0.0049 | 0.0001 | 0.0104 | 0.0005 |
Mitochondria | 0.0145 | 0.0011 | 0.0038 | 0.0048 | 0.0715 | 0.0075 | 0.0074 | 0.0006 | 0.0052 | 0.0217 | 0.0175 | 0.008 | 0.0013 | 0 | 0.0013 | 0 | 0.0034 | 0.0027 |
None | 0.7955 | 0.9656 | 0.9174 | 0.8949 | 0.6558 | 0.9218 | 0.7741 | 0.8973 | 0.891 | 0.8515 | 0.6549 | 0.8808 | 0.9053 | 0.964 | 0.9052 | 0.9913 | 0.6846 | 0.8979 |
Nuclear Periphery | 0.0096 | 0.0003 | 0.0035 | 0.0002 | 0.01 | 0.0014 | 0.0066 | 0.0002 | 0.0008 | 0.0009 | 0.0131 | 0.0052 | 0.0017 | 0.0001 | 0.0072 | 0.0001 | 0.021 | 0.0004 |
Nucleolus | 0.0024 | 0.0012 | 0.0005 | 0.0001 | 0.0014 | 0.002 | 0.0009 | 0.0005 | 0.0002 | 0.0002 | 0.0033 | 0.0002 | 0.0004 | 0 | 0.0001 | 0 | 0.0029 | 0.0002 |
Nucleus | 0.0061 | 0.0008 | 0.003 | 0.0001 | 0.0044 | 0.0013 | 0.0062 | 0.0006 | 0.0004 | 0.0007 | 0.012 | 0.0008 | 0.0005 | 0.0001 | 0.0013 | 0 | 0.0374 | 0.0005 |
Peroxisomes | 0.0178 | 0.0003 | 0.0071 | 0.0015 | 0.025 | 0.0016 | 0.0096 | 0.0005 | 0.0055 | 0.0009 | 0.0187 | 0.0041 | 0.0051 | 0 | 0.0077 | 0 | 0.0041 | 0.0003 |
Punctate Nuclear | 0.0099 | 0.0018 | 0.0018 | 0.0002 | 0.0117 | 0.0074 | 0.0078 | 0.0006 | 0.0011 | 0.0008 | 0.0099 | 0.002 | 0.0019 | 0.0001 | 0.0003 | 0.0001 | 0.0186 | 0.0004 |
Vacuole | 0.0023 | 0.0006 | 0.0017 | 0.0007 | 0.002 | 0.0004 | 0.0087 | 0.0013 | 0.0015 | 0.0022 | 0.0147 | 0.0008 | 0.0005 | 0.0001 | 0.0009 | 0 | 0.0022 | 0.0011 |
Vacuole Periphery | 0.0016 | 0.0001 | 0.0005 | 0.0007 | 0.0016 | 0.0002 | 0.0018 | 0.0001 | 0.0006 | 0.0016 | 0.003 | 0.0007 | 0.0003 | 0 | 0.0007 | 0 | 0.0011 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 21.8655 | 20.5752 | 26.8712 | 32.32 | 25.067 | 21.6234 | 27.7728 | 29.3457 | 27.6315 | 31.4237 |
Translational Efficiency | 1.3783 | 1.6802 | 1.2345 | 1.021 | 1.2209 | 1.3084 | 1.143 | 0.84 | 1.063 | 1.0503 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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