| Standard name | |
|---|---|
| Description | Subunit (90 kDa) of TFIID and SAGA complexes; involved in RNA polymerase II transcription initiation and in chromatin modification |
Micrographs
Sub-cellular Localization
Yeast GFP Assignment
Protein Abundance
Localization Change
External localization resources
| ensLOC | DeepLoc | |||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
| Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | – | 1 | 3 | 4 | 2 | 6 | 2 |
| Bud | 0 | 2 | 0 | 0 | 0 | 0 | 3 | 4 | 2 | 5 | 9 | 4 | 0 | 1 | 0 | 0 | 0 | – | 1 | 0 | 1 | 3 | 4 | 5 |
| Bud Neck | 0 | 1 | 5 | 2 | 7 | 5 | 14 | 18 | 2 | 9 | 9 | 12 | 0 | 0 | 0 | 3 | 11 | – | 0 | 1 | 1 | 2 | 2 | 4 |
| Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – | – |
| Cell Periphery | 0 | 0 | 2 | 3 | 3 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 2 | 4 | – | 1 | 1 | 3 | 3 | 1 | 2 |
| Cytoplasm | 124 | 199 | 271 | 199 | 340 | 200 | 316 | 290 | 152 | 139 | 199 | 255 | 161 | 269 | 254 | 492 | 518 | – | 110 | 149 | 164 | 96 | 161 | 182 |
| Endoplasmic Reticulum | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 49 | 25 | – | 2 | 0 | 0 | 2 | 1 | 6 |
| Endosome | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 4 | – | 4 | 4 | 1 | 2 | 1 | 0 |
| Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | – | 0 | 0 | 0 | 1 | 2 | 0 |
| Mitochondria | 19 | 19 | 1 | 3 | 2 | 9 | 30 | 25 | 46 | 55 | 77 | 39 | 8 | 1 | 0 | 26 | 9 | – | 16 | 21 | 30 | 15 | 34 | 23 |
| Nucleus | 0 | 1 | 1 | 2 | 2 | 0 | 2 | 4 | 3 | 3 | 1 | 1 | 2 | 1 | 0 | 3 | 2 | – | 0 | 0 | 0 | 0 | 0 | 0 |
| Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
| Nucleolus | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 |
| Peroxisomes | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 1 |
| SpindlePole | 0 | 0 | 2 | 0 | 1 | 3 | 7 | 4 | 3 | 4 | 9 | 10 | 0 | 0 | 0 | 0 | 0 | – | 0 | 3 | 1 | 1 | 4 | 2 |
| Vac/Vac Membrane | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 2 | 0 | 1 | 1 | 3 | 4 | – | 2 | 7 | 4 | 5 | 8 | 18 |
| Unique Cell Count | 137 | 213 | 277 | 204 | 345 | 210 | 333 | 306 | 178 | 179 | 250 | 285 | 163 | 271 | 256 | 502 | 530 | 147 | 203 | 234 | 142 | 239 | 257 | |
| Labelled Cell Count | 144 | 224 | 284 | 210 | 355 | 218 | 372 | 350 | 209 | 215 | 307 | 325 | 172 | 275 | 257 | 581 | 582 | 147 | 203 | 234 | 142 | 239 | 257 | |
Yeast GFP Assignment
Protein Abundance
| Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Mean Cell GFP Intensity (1e-4) | 3.3 | 2.9 | 4.2 | 3.9 | 4.6 | 3.5 | 3.4 | 3.8 | 3.2 | 2.9 | 2.7 | 3.5 | 3.6 | 3.8 | 4.1 | 5.1 | 5.1 | 4.8 | 3.5 | 3.5 | 4.1 |
| Std Deviation (1e-4) | 0.4 | 0.5 | 0.9 | 1.4 | 1.8 | 1.2 | 1.0 | 1.1 | 1.4 | 0.7 | 0.6 | 1.6 | 0.7 | 1.3 | 1.7 | 1.2 | 1.0 | 0.8 | 1.4 | 1.3 | 2.0 |
| Intensity Change (Log2) | – | – | – | -0.1 | 0.14 | -0.28 | -0.31 | -0.14 | -0.41 | -0.54 | -0.62 | -0.26 | -0.21 | -0.13 | -0.04 | 0.29 | 0.27 | 0.2 | -0.25 | -0.28 | -0.02 |
Localization Change
| Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
External localization resources
