Standard name | |
---|---|
Human Ortholog | |
Description | Component of the Mcm2-7 hexameric helicase complex; MCM2-7 primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 1 | 0 | 2 | 0 | 1 | 1 | 0 | – | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 4 | 1 | 4 | 3 | 6 | 5 | 9 | 10 | 14 | – | 9 | 9 | 0 | 0 | 0 | 0 | 0 | 0 | 15 | 22 | 30 | 20 | 7 | 9 |
Bud Neck | 0 | 0 | 0 | 0 | 2 | 3 | 2 | 8 | 2 | – | 2 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 2 | 1 | 4 | 3 |
Bud Site | 0 | 0 | 0 | 0 | 1 | 3 | 1 | 0 | 6 | – | 5 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 1 | 1 | 0 | 2 | 4 | 8 | 2 | – | 2 | 3 | 0 | 1 | 1 | 6 | 4 | 6 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 61 | 58 | 97 | 69 | 172 | 117 | 125 | 123 | 80 | – | 54 | 69 | 33 | 90 | 115 | 181 | 99 | 97 | 39 | 31 | 53 | 18 | 3 | 1 |
Endoplasmic Reticulum | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 1 | 0 | 1 | 1 | 12 | 6 | 5 | 0 | 0 | 0 | 1 | 0 | 1 |
Endosome | 7 | 5 | 3 | 6 | 12 | 12 | 8 | 10 | 4 | – | 0 | 1 | 6 | 18 | 28 | 4 | 7 | 5 | 8 | 3 | 6 | 2 | 15 | 37 |
Golgi | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 1 | 1 | 1 | 0 | 0 | 4 | 3 |
Mitochondria | 7 | 2 | 1 | 1 | 4 | 31 | 26 | 21 | 84 | – | 73 | 42 | 3 | 1 | 3 | 3 | 0 | 1 | 1 | 0 | 2 | 0 | 4 | 10 |
Nucleus | 82 | 139 | 117 | 143 | 151 | 157 | 226 | 239 | 191 | – | 150 | 200 | 288 | 281 | 240 | 169 | 102 | 92 | 104 | 143 | 121 | 167 | 205 | 204 |
Nuclear Periphery | 1 | 1 | 0 | 1 | 5 | 14 | 17 | 24 | 21 | – | 4 | 5 | 0 | 5 | 12 | 3 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 13 |
Nucleolus | 22 | 21 | 5 | 18 | 38 | 59 | 68 | 72 | 86 | – | 66 | 74 | 39 | 111 | 118 | 14 | 11 | 5 | 29 | 21 | 13 | 43 | 172 | 210 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 2 | 3 | 3 | 4 | 11 | 2 | – | 0 | 0 | 0 | 1 | 0 | 2 | 2 | 1 | 4 | 9 | 8 | 7 | 10 | 23 |
Vac/Vac Membrane | 17 | 14 | 28 | 12 | 35 | 17 | 17 | 31 | 10 | – | 9 | 13 | 26 | 47 | 76 | 51 | 32 | 38 | 8 | 7 | 8 | 16 | 88 | 119 |
Unique Cell Count | 184 | 232 | 242 | 245 | 397 | 371 | 452 | 490 | 393 | 297 | 363 | 373 | 523 | 551 | 412 | 241 | 228 | 220 | 245 | 252 | 281 | 526 | 649 | |
Labelled Cell Count | 201 | 243 | 257 | 258 | 431 | 423 | 509 | 559 | 502 | 375 | 425 | 396 | 556 | 595 | 447 | 264 | 252 | 220 | 245 | 252 | 281 | 526 | 649 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 11.8 | 12.3 | 10.1 | 9.9 | 9.2 | 7.6 | 7.5 | 8.2 | 6.7 | – | 6.3 | 6.5 | 13.2 | 13.4 | 13.0 | 15.5 | 16.0 | 15.0 | 11.2 | 11.3 | 11.7 |
Std Deviation (1e-4) | 3.5 | 3.2 | 2.0 | 2.2 | 1.9 | 1.9 | 1.9 | 2.0 | 1.9 | – | 1.8 | 1.6 | 3.1 | 2.9 | 2.8 | 4.6 | 4.9 | 4.7 | 2.7 | 2.3 | 2.1 |
Intensity Change (Log2) | – | – | – | -0.03 | -0.13 | -0.41 | -0.43 | -0.3 | -0.59 | – | -0.69 | -0.63 | 0.39 | 0.41 | 0.37 | 0.62 | 0.66 | 0.57 | 0.15 | 0.16 | 0.21 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 1.4 | – | 1.0 | 0.7 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -2.8 | 0.8 | -2.2 | -3.3 | -4.2 | -5.4 | – | -5.6 | -5.7 | -9.3 | -6.8 | -5.6 | 1.0 | 0.2 | 0.5 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 1.4 | 1.6 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1.7 | 2.6 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 4.3 | 3.5 | 2.9 | 7.5 | – | 8.1 | 5.2 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 2.2 | -2.6 | -1.5 | 0.4 | 0.1 | 0.1 | – | 0.5 | 1.6 | 7.4 | 1.4 | -1.2 | -1.8 | -1.3 | -1.7 |
Nuclear Periphery | 0 | 0 | 3.1 | 3.1 | 3.5 | 3.7 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 2.7 | 3.7 | 5.5 | 5.3 | 5.2 | 6.9 | – | 6.9 | 6.6 | 3.9 | 6.9 | 6.9 | 1.0 | 1.5 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -2.7 | -1.1 | -3.2 | -4.0 | -2.5 | -4.7 | – | -3.9 | -3.8 | -2.0 | -1.1 | 0.9 | 0.3 | 0.6 | 1.6 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 9.9669 | 9.373 | 8.5317 | 7.3051 | 6.9124 | 9.5968 | 7.0106 | 8.1753 | 6.1015 | 4.4311 | 5.0125 | 7.8467 | 8.1131 | 9.637 | 7.2308 | 5.5732 | 5.225 | 8.792 |
Actin | 0.0022 | 0.001 | 0.0125 | 0.0031 | 0.0062 | 0.0026 | 0.0305 | 0.0118 | 0.0097 | 0.0081 | 0.0192 | 0.0004 | 0.0118 | 0.003 | 0.0076 | 0.0465 | 0.006 | 0.0144 |
Bud | 0.0012 | 0.0206 | 0.0466 | 0.0673 | 0.0422 | 0.0121 | 0.001 | 0.0322 | 0.0596 | 0.0496 | 0.0411 | 0.0006 | 0.0004 | 0.0112 | 0.034 | 0.0427 | 0.0451 | 0.0014 |
Bud Neck | 0.0013 | 0.0043 | 0.0063 | 0.0071 | 0.0155 | 0.0114 | 0.0005 | 0.0016 | 0.0034 | 0.0055 | 0.008 | 0.0061 | 0.0008 | 0.0016 | 0.0048 | 0.0067 | 0.0024 | 0.0045 |
Bud Periphery | 0.0025 | 0.0131 | 0.0405 | 0.0646 | 0.0443 | 0.0123 | 0.001 | 0.0142 | 0.0327 | 0.0333 | 0.0174 | 0.0009 | 0.0005 | 0.0047 | 0.0201 | 0.044 | 0.0408 | 0.0014 |
Bud Site | 0.0043 | 0.0626 | 0.0296 | 0.0392 | 0.0197 | 0.0052 | 0.002 | 0.0383 | 0.0204 | 0.0114 | 0.0353 | 0.0008 | 0.0068 | 0.0139 | 0.0129 | 0.0093 | 0.003 | 0.0004 |
Cell Periphery | 0.0009 | 0.0022 | 0.0027 | 0.0038 | 0.0024 | 0.0007 | 0.0002 | 0.0014 | 0.0014 | 0.0024 | 0.0018 | 0.0007 | 0.0002 | 0.0005 | 0.0013 | 0.0015 | 0.0005 | 0.0002 |
Cytoplasm | 0.0122 | 0.0708 | 0.3499 | 0.6163 | 0.6386 | 0.053 | 0.0192 | 0.1562 | 0.4001 | 0.6208 | 0.525 | 0.0764 | 0.0097 | 0.0797 | 0.3823 | 0.45 | 0.2583 | 0.0581 |
Cytoplasmic Foci | 0.0021 | 0.0177 | 0.0085 | 0.0091 | 0.0089 | 0.0028 | 0.009 | 0.0025 | 0.0192 | 0.0236 | 0.0127 | 0.0013 | 0.0253 | 0.002 | 0.0208 | 0.0446 | 0.0298 | 0.001 |
Eisosomes | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0.0002 | 0 | 0 | 0 | 0.0002 | 0 | 0.0004 | 0 | 0.0003 | 0.0002 | 0.0002 | 0 |
Endoplasmic Reticulum | 0.0018 | 0.0015 | 0.0126 | 0.0121 | 0.0067 | 0.0009 | 0.0048 | 0.0012 | 0.0046 | 0.0044 | 0.002 | 0.0005 | 0.0026 | 0.0014 | 0.0055 | 0.0074 | 0.0021 | 0.0016 |
Endosome | 0.0035 | 0.0066 | 0.0079 | 0.0154 | 0.0067 | 0.0026 | 0.0266 | 0.0018 | 0.022 | 0.0424 | 0.0388 | 0.0014 | 0.0133 | 0.0011 | 0.0113 | 0.022 | 0.0152 | 0.0058 |
Golgi | 0.001 | 0.0006 | 0.0011 | 0.0012 | 0.0007 | 0.0009 | 0.008 | 0.0007 | 0.004 | 0.0495 | 0.0197 | 0.0003 | 0.007 | 0.0002 | 0.002 | 0.0066 | 0.0105 | 0.0009 |
Lipid Particles | 0.0034 | 0.0007 | 0.0018 | 0.0006 | 0.0005 | 0.0038 | 0.0067 | 0.0002 | 0.0019 | 0.003 | 0.0063 | 0.0003 | 0.0213 | 0.0001 | 0.0087 | 0.0111 | 0.1064 | 0.0013 |
Mitochondria | 0.0014 | 0.0006 | 0.0041 | 0.0025 | 0.0015 | 0.0013 | 0.0066 | 0.0011 | 0.0063 | 0.0068 | 0.0161 | 0.0091 | 0.0131 | 0.0011 | 0.008 | 0.0059 | 0.1518 | 0.0167 |
None | 0.001 | 0.001 | 0.0034 | 0.0026 | 0.0283 | 0.0015 | 0.0043 | 0.0006 | 0.0024 | 0.0039 | 0.0012 | 0.0003 | 0.0028 | 0.004 | 0.0087 | 0.0159 | 0.0133 | 0.0004 |
Nuclear Periphery | 0.0193 | 0.0142 | 0.0337 | 0.008 | 0.0119 | 0.0059 | 0.0209 | 0.0201 | 0.0235 | 0.0122 | 0.0024 | 0.007 | 0.0082 | 0.0103 | 0.0206 | 0.0164 | 0.0066 | 0.007 |
Nucleolus | 0.031 | 0.0342 | 0.0284 | 0.011 | 0.0034 | 0.1439 | 0.0201 | 0.0168 | 0.026 | 0.0108 | 0.0868 | 0.1412 | 0.0287 | 0.0109 | 0.0163 | 0.0035 | 0.0092 | 0.0822 |
Nucleus | 0.8944 | 0.7085 | 0.3553 | 0.0777 | 0.1361 | 0.7206 | 0.8073 | 0.6861 | 0.3229 | 0.0703 | 0.1391 | 0.7416 | 0.8088 | 0.8253 | 0.3946 | 0.2285 | 0.231 | 0.7984 |
Peroxisomes | 0.0078 | 0.0003 | 0.0059 | 0.0004 | 0.0004 | 0.0008 | 0.0054 | 0.0003 | 0.0007 | 0.0016 | 0.0021 | 0.0001 | 0.0047 | 0.0002 | 0.0035 | 0.0071 | 0.0383 | 0.0003 |
Punctate Nuclear | 0.0014 | 0.0258 | 0.0199 | 0.0084 | 0.0038 | 0.0121 | 0.0191 | 0.0063 | 0.0228 | 0.0057 | 0.0014 | 0.0054 | 0.0312 | 0.0227 | 0.0207 | 0.0096 | 0.0092 | 0.0016 |
Vacuole | 0.0055 | 0.0127 | 0.0273 | 0.0447 | 0.0198 | 0.0048 | 0.004 | 0.0057 | 0.0134 | 0.0298 | 0.0199 | 0.0052 | 0.0014 | 0.0055 | 0.0129 | 0.0174 | 0.0136 | 0.0015 |
Vacuole Periphery | 0.0016 | 0.0009 | 0.0022 | 0.0049 | 0.0023 | 0.0007 | 0.0025 | 0.0009 | 0.0031 | 0.0047 | 0.0035 | 0.0006 | 0.001 | 0.0007 | 0.0031 | 0.0034 | 0.0068 | 0.0009 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 15.6511 | 12.0479 | 63.7342 | 69.9933 | 20.856 | 20.4221 | 27.2122 | 55.1656 | 58.0073 | 35.4332 |
Translational Efficiency | 0.5524 | 0.7554 | 0.8925 | 0.8534 | 0.8932 | 0.7713 | 0.8169 | 0.7416 | 0.7951 | 0.7289 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✔ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1311 | 727 | 1574 | 811 | 1028 | 1493 | 953 | 614 | 2339 | 2220 | 2527 | 1425 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 921.20 | 1184.98 | 1705.32 | 1550.90 | 1110.48 | 1178.37 | 1483.56 | 1452.66 | 1004.39 | 1180.53 | 1621.69 | 1508.57 |
Standard Deviation | 163.53 | 242.13 | 326.49 | 299.27 | 210.76 | 233.45 | 256.18 | 336.86 | 208.17 | 236.35 | 320.46 | 319.74 |
Intensity Change Log 2 | — | 0.363276 | 0.888456 | 0.751519 | — | 0.085609 | 0.417880 | 0.387514 | — | 0.218160 | 0.650377 | 0.564000 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000602 | 0.000988 | 0.000662 | 0.000655 | 0.000920 | 0.000961 | 0.000959 | 0.000468 | 0.000742 | 0.000970 | 0.000774 | 0.000574 |
Bud Neck | 0.016181 | 0.040745 | 0.046182 | 0.010423 | 0.029511 | 0.042000 | 0.029060 | 0.013144 | 0.022040 | 0.041589 | 0.039725 | 0.011595 |
Bud Site | 0.011736 | 0.013885 | 0.006795 | 0.016916 | 0.012579 | 0.014310 | 0.010113 | 0.018222 | 0.012106 | 0.014171 | 0.008046 | 0.017479 |
Cell Periphery | 0.001010 | 0.000607 | 0.000373 | 0.000806 | 0.000789 | 0.000664 | 0.000486 | 0.000454 | 0.000913 | 0.000646 | 0.000416 | 0.000654 |
Cytoplasm | 0.133269* | 0.146871* | 0.126502* | 0.636998* | 0.124010* | 0.123510* | 0.259202* | 0.592063* | 0.129200* | 0.131160* | 0.176547* | 0.617637* |
Cytoplasmic Foci | 0.016877 | 0.011019 | 0.004927 | 0.022932 | 0.017835 | 0.015881 | 0.016174 | 0.021046 | 0.017298 | 0.014289 | 0.009168 | 0.022119 |
Eisosomes | 0.000049 | 0.000035 | 0.000026 | 0.000008 | 0.000047 | 0.000046 | 0.000030 | 0.000009 | 0.000048 | 0.000043 | 0.000028 | 0.000009 |
Endoplasmic Reticulum | 0.002382 | 0.001747 | 0.000703 | 0.000423 | 0.002715 | 0.002552 | 0.000961 | 0.000337 | 0.002528 | 0.002288 | 0.000800 | 0.000386 |
Endosome | 0.003885 | 0.002182 | 0.000585 | 0.002524 | 0.004455 | 0.003495 | 0.001414 | 0.001549 | 0.004135 | 0.003065 | 0.000898 | 0.002104 |
Golgi | 0.001495 | 0.000758 | 0.000464 | 0.004402 | 0.001052 | 0.002224 | 0.000508 | 0.000763 | 0.001300 | 0.001744 | 0.000481 | 0.002834 |
Lipid Particles | 0.000490 | 0.000392 | 0.000558 | 0.003369 | 0.000551 | 0.000875 | 0.000793 | 0.001044 | 0.000517 | 0.000717 | 0.000647 | 0.002367 |
Mitochondria | 0.001730 | 0.001847 | 0.002384 | 0.000854 | 0.001804 | 0.003468 | 0.002001 | 0.000872 | 0.001762 | 0.002937 | 0.002240 | 0.000862 |
Mitotic Spindle | 0.007306 | 0.017165 | 0.016489 | 0.034825 | 0.007147 | 0.019441 | 0.028019 | 0.039862 | 0.007236 | 0.018696 | 0.020837 | 0.036995 |
None | 0.011169 | 0.004737 | 0.003663 | 0.005806 | 0.005575 | 0.005185 | 0.004545 | 0.007485 | 0.008710 | 0.005038 | 0.003996 | 0.006529 |
Nuclear Periphery | 0.000455 | 0.000773 | 0.000832 | 0.001619 | 0.000684 | 0.001232 | 0.000822 | 0.000583 | 0.000556 | 0.001082 | 0.000829 | 0.001173 |
Nuclear Periphery Foci | 0.004263 | 0.003047 | 0.000892 | 0.005428 | 0.004257 | 0.003371 | 0.003336 | 0.002273 | 0.004260 | 0.003265 | 0.001814 | 0.004069 |
Nucleolus | 0.196762 | 0.123366 | 0.012627 | 0.003234 | 0.157074 | 0.138762 | 0.019209 | 0.003796 | 0.179319 | 0.133720 | 0.015110 | 0.003476 |
Nucleus | 0.577700* | 0.619886* | 0.768651* | 0.214318* | 0.615828* | 0.611425* | 0.600494* | 0.278470* | 0.594458* | 0.614196* | 0.705234* | 0.241960* |
Peroxisomes | 0.001713 | 0.001465 | 0.000464 | 0.000366 | 0.001808 | 0.001631 | 0.000737 | 0.000471 | 0.001755 | 0.001576 | 0.000567 | 0.000411 |
Vacuole | 0.009285 | 0.007196 | 0.005837 | 0.029956 | 0.009611 | 0.007192 | 0.020758 | 0.016643 | 0.009428 | 0.007193 | 0.011464 | 0.024219 |
Vacuole Periphery | 0.001639 | 0.001287 | 0.000383 | 0.004137 | 0.001748 | 0.001776 | 0.000378 | 0.000446 | 0.001687 | 0.001616 | 0.000381 | 0.002546 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -4.39 | -7.10 | -7.89 | -0.98 | -4.17 | -0.82 | -3.57 | 0.02 | 0.77 | 3.23 | -4.66 | -6.59 | -6.17 | -0.81 | -1.70 |
Bud Neck | -9.39 | -17.59 | -7.34 | 2.35 | 5.74 | -4.56 | -3.58 | -0.82 | 2.93 | 2.26 | -11.17 | -14.84 | -5.83 | 3.68 | 6.11 |
Bud Site | -2.61 | 3.31 | -5.66 | -2.38 | -8.90 | -1.54 | 1.54 | -3.05 | -2.06 | -4.15 | -3.14 | 3.79 | -6.16 | -3.44 | -9.31 |
Cell Periphery | 2.66 | 5.37 | 1.98 | -1.26 | -5.82 | 1.15 | 2.80 | 2.19 | 1.44 | -1.13 | 2.87 | 5.97 | 2.88 | -0.28 | -5.04 |
Cytoplasm | -0.25 | 3.40 | -16.44 | -14.80 | -19.60 | 0.16 | -5.23 | -14.37 | -15.00 | -10.34 | 0.31 | -0.74 | -21.85 | -22.02 | -21.86 |
Cytoplasmic Foci | 2.70 | 6.06 | -1.93 | -4.66 | -8.50 | 0.89 | 2.42 | -0.11 | -1.11 | -2.92 | 1.94 | 6.33 | -1.58 | -3.61 | -8.75 |
Eisosomes | 5.02 | 6.84 | 10.42 | 5.94 | 5.23 | 0.22 | 3.74 | 10.26 | 10.77 | 7.42 | 2.78 | 7.85 | 14.51 | 12.40 | 8.69 |
Endoplasmic Reticulum | 1.52 | 5.92 | 3.85 | 3.56 | -4.21 | 0.08 | 5.58 | 6.34 | 6.87 | 2.09 | 0.27 | 8.28 | 6.90 | 7.38 | -2.19 |
Endosome | 3.46 | 9.42 | 4.41 | 1.04 | -3.56 | 1.57 | 5.32 | 4.51 | 4.20 | -0.21 | 2.73 | 10.01 | 6.29 | 4.12 | -3.00 |
Golgi | 1.62 | 2.53 | -0.43 | -1.24 | -1.64 | -2.10 | 2.57 | 1.38 | 3.03 | -1.64 | -0.78 | 3.30 | -0.20 | 0.30 | -1.80 |
Lipid Particles | -0.32 | -1.33 | -6.78 | -6.49 | -6.30 | -0.75 | -1.96 | -4.40 | -2.74 | -3.55 | -0.97 | -2.08 | -7.99 | -6.24 | -7.20 |
Mitochondria | -0.88 | -4.38 | -1.68 | -1.19 | 5.45 | -2.24 | -3.83 | -1.38 | 1.74 | 3.14 | -2.40 | -5.80 | -2.15 | 1.46 | 6.35 |
Mitotic Spindle | -3.87 | -5.46 | -10.12 | -6.93 | -7.83 | -4.94 | -6.98 | -9.10 | -7.12 | -4.65 | -6.53 | -8.79 | -13.60 | -10.08 | -9.06 |
None | 5.83 | 7.35 | 4.40 | -2.65 | -5.96 | 0.37 | 0.80 | -2.06 | -3.14 | -3.73 | 5.20 | 6.85 | 2.45 | -4.00 | -6.89 |
Nuclear Periphery | -4.18 | -8.22 | -6.92 | -5.38 | -4.69 | -2.21 | -5.01 | -6.97 | -3.88 | -3.50 | -3.62 | -8.96 | -9.41 | -5.85 | -5.91 |
Nuclear Periphery Foci | -0.35 | 3.50 | -1.45 | -0.94 | -5.29 | 0.42 | 1.98 | 0.94 | 0.58 | -0.82 | 0.07 | 4.17 | -0.68 | -0.76 | -5.02 |
Nucleolus | 7.87 | 27.21* | 27.02* | 14.87 | 1.37 | 1.96 | 17.70 | 21.00 | 22.66* | 4.90 | 7.23 | 32.42* | 34.09* | 27.17 | 4.21 |
Nucleus | -2.10 | -16.04 | 14.64 | 14.68 | 27.89 | 0.97 | -0.37 | 12.70 | 12.63 | 12.41 | -0.98 | -12.29 | 19.39 | 19.96 | 29.27 |
Peroxisomes | 1.25 | 7.63 | 6.45 | 4.40 | -1.00 | 0.77 | 5.93 | 6.30 | 4.22 | 1.65 | 1.02 | 9.81 | 9.03 | 5.60 | 0.36 |
Vacuole | 1.07 | -0.94 | -10.64 | -11.14 | -10.23 | 0.97 | -7.09 | -7.50 | -7.92 | -1.76 | 1.26 | -6.00 | -12.98 | -13.57 | -9.05 |
Vacuole Periphery | 1.48 | 6.84 | -0.05 | -0.56 | -1.79 | 0.14 | 5.06 | 4.90 | 5.73 | 0.30 | 0.59 | 8.32 | 1.30 | 1.00 | -1.75 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|