Standard name | |
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Description | Component of the Mcm2-7 hexameric helicase complex; MCM2-7 primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 1 | 0 | 2 | 0 | 1 | 1 | 0 | – | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 4 | 1 | 4 | 3 | 6 | 5 | 9 | 10 | 14 | – | 9 | 9 | 0 | 0 | 0 | 0 | 0 | 0 | 15 | 22 | 30 | 20 | 7 | 9 |
Bud Neck | 0 | 0 | 0 | 0 | 2 | 3 | 2 | 8 | 2 | – | 2 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 2 | 1 | 4 | 3 |
Bud Site | 0 | 0 | 0 | 0 | 1 | 3 | 1 | 0 | 6 | – | 5 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 1 | 1 | 0 | 2 | 4 | 8 | 2 | – | 2 | 3 | 0 | 1 | 1 | 6 | 4 | 6 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 61 | 58 | 97 | 69 | 172 | 117 | 125 | 123 | 80 | – | 54 | 69 | 33 | 90 | 115 | 181 | 99 | 97 | 39 | 31 | 53 | 18 | 3 | 1 |
Endoplasmic Reticulum | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 1 | 0 | 1 | 1 | 12 | 6 | 5 | 0 | 0 | 0 | 1 | 0 | 1 |
Endosome | 7 | 5 | 3 | 6 | 12 | 12 | 8 | 10 | 4 | – | 0 | 1 | 6 | 18 | 28 | 4 | 7 | 5 | 8 | 3 | 6 | 2 | 15 | 37 |
Golgi | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 1 | 1 | 1 | 0 | 0 | 4 | 3 |
Mitochondria | 7 | 2 | 1 | 1 | 4 | 31 | 26 | 21 | 84 | – | 73 | 42 | 3 | 1 | 3 | 3 | 0 | 1 | 1 | 0 | 2 | 0 | 4 | 10 |
Nucleus | 82 | 139 | 117 | 143 | 151 | 157 | 226 | 239 | 191 | – | 150 | 200 | 288 | 281 | 240 | 169 | 102 | 92 | 104 | 143 | 121 | 167 | 205 | 204 |
Nuclear Periphery | 1 | 1 | 0 | 1 | 5 | 14 | 17 | 24 | 21 | – | 4 | 5 | 0 | 5 | 12 | 3 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 13 |
Nucleolus | 22 | 21 | 5 | 18 | 38 | 59 | 68 | 72 | 86 | – | 66 | 74 | 39 | 111 | 118 | 14 | 11 | 5 | 29 | 21 | 13 | 43 | 172 | 210 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 2 | 3 | 3 | 4 | 11 | 2 | – | 0 | 0 | 0 | 1 | 0 | 2 | 2 | 1 | 4 | 9 | 8 | 7 | 10 | 23 |
Vac/Vac Membrane | 17 | 14 | 28 | 12 | 35 | 17 | 17 | 31 | 10 | – | 9 | 13 | 26 | 47 | 76 | 51 | 32 | 38 | 8 | 7 | 8 | 16 | 88 | 119 |
Unique Cell Count | 184 | 232 | 242 | 245 | 397 | 371 | 452 | 490 | 393 | 297 | 363 | 373 | 523 | 551 | 412 | 241 | 228 | 220 | 245 | 252 | 281 | 526 | 649 | |
Labelled Cell Count | 201 | 243 | 257 | 258 | 431 | 423 | 509 | 559 | 502 | 375 | 425 | 396 | 556 | 595 | 447 | 264 | 252 | 220 | 245 | 252 | 281 | 526 | 649 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
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Mean Cell GFP Intensity (1e-4) | 11.8 | 12.3 | 10.1 | 9.9 | 9.2 | 7.6 | 7.5 | 8.2 | 6.7 | – | 6.3 | 6.5 | 13.2 | 13.4 | 13.0 | 15.5 | 16.0 | 15.0 | 11.2 | 11.3 | 11.7 |
Std Deviation (1e-4) | 3.5 | 3.2 | 2.0 | 2.2 | 1.9 | 1.9 | 1.9 | 2.0 | 1.9 | – | 1.8 | 1.6 | 3.1 | 2.9 | 2.8 | 4.6 | 4.9 | 4.7 | 2.7 | 2.3 | 2.1 |
Intensity Change (Log2) | – | – | – | -0.03 | -0.13 | -0.41 | -0.43 | -0.3 | -0.59 | – | -0.69 | -0.63 | 0.39 | 0.41 | 0.37 | 0.62 | 0.66 | 0.57 | 0.15 | 0.16 | 0.21 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
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Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 1.4 | – | 1.0 | 0.7 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -2.8 | 0.8 | -2.2 | -3.3 | -4.2 | -5.4 | – | -5.6 | -5.7 | -9.3 | -6.8 | -5.6 | 1.0 | 0.2 | 0.5 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 1.4 | 1.6 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1.7 | 2.6 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 4.3 | 3.5 | 2.9 | 7.5 | – | 8.1 | 5.2 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 2.2 | -2.6 | -1.5 | 0.4 | 0.1 | 0.1 | – | 0.5 | 1.6 | 7.4 | 1.4 | -1.2 | -1.8 | -1.3 | -1.7 |
Nuclear Periphery | 0 | 0 | 3.1 | 3.1 | 3.5 | 3.7 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 2.7 | 3.7 | 5.5 | 5.3 | 5.2 | 6.9 | – | 6.9 | 6.6 | 3.9 | 6.9 | 6.9 | 1.0 | 1.5 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -2.7 | -1.1 | -3.2 | -4.0 | -2.5 | -4.7 | – | -3.9 | -3.8 | -2.0 | -1.1 | 0.9 | 0.3 | 0.6 | 1.6 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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