Standard name
Description Component of the Mcm2-7 hexameric helicase complex; MCM2-7 primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.07 0.09 0.12 0.07 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.33 0.25 0.4 0.28 0.43 0.32 0.28 0.25 0.2 – 0.18 0.19 0.09 0.17 0.21 0.44 0.41 0.43 0.18 0.13 0.21 0.06 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0.06
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.08 0.06 0 0.21 – 0.25 0.12 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.45 0.6 0.48 0.58 0.38 0.42 0.5 0.49 0.49 – 0.51 0.55 0.77 0.54 0.44 0.41 0.42 0.4 0.47 0.59 0.48 0.59 0.39 0.31
Nuclear Periphery 0 0 0 0 0 0 0 0.05 0.05 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.12 0.09 0 0.07 0.1 0.16 0.15 0.15 0.22 – 0.22 0.2 0.1 0.21 0.21 0 0.05 0 0.13 0.09 0.05 0.16 0.33 0.32
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.09 0.06 0.12 0.05 0.09 0.05 0 0.06 0 – 0 0 0.07 0.09 0.14 0.12 0.13 0.17 0 0 0 0.06 0.17 0.18
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 2 0 1 1 0 – 1 0 0 0 1 0 0 0 0 0 0 0 0 0
Bud 4 1 4 3 6 5 9 10 14 – 9 9 0 0 0 0 0 0 15 22 30 20 7 9
Bud Neck 0 0 0 0 2 3 2 8 2 – 2 3 1 0 0 0 0 0 2 2 2 1 4 3
Bud Site 0 0 0 0 1 3 1 0 6 – 5 5 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 1 1 0 2 4 8 2 – 2 3 0 1 1 6 4 6 0 0 0 0 0 0
Cytoplasm 61 58 97 69 172 117 125 123 80 – 54 69 33 90 115 181 99 97 39 31 53 18 3 1
Endoplasmic Reticulum 0 1 0 0 0 0 1 0 0 – 0 1 0 1 1 12 6 5 0 0 0 1 0 1
Endosome 7 5 3 6 12 12 8 10 4 – 0 1 6 18 28 4 7 5 8 3 6 2 15 37
Golgi 0 0 0 2 0 0 0 1 0 – 0 0 0 0 0 2 1 1 1 1 0 0 4 3
Mitochondria 7 2 1 1 4 31 26 21 84 – 73 42 3 1 3 3 0 1 1 0 2 0 4 10
Nucleus 82 139 117 143 151 157 226 239 191 – 150 200 288 281 240 169 102 92 104 143 121 167 205 204
Nuclear Periphery 1 1 0 1 5 14 17 24 21 – 4 5 0 5 12 3 0 1 0 0 0 0 2 13
Nucleolus 22 21 5 18 38 59 68 72 86 – 66 74 39 111 118 14 11 5 29 21 13 43 172 210
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 2 3 3 4 11 2 – 0 0 0 1 0 2 2 1 4 9 8 7 10 23
Vac/Vac Membrane 17 14 28 12 35 17 17 31 10 – 9 13 26 47 76 51 32 38 8 7 8 16 88 119
Unique Cell Count 184 232 242 245 397 371 452 490 393 297 363 373 523 551 412 241 228 220 245 252 281 526 649
Labelled Cell Count 201 243 257 258 431 423 509 559 502 375 425 396 556 595 447 264 252 220 245 252 281 526 649


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.8 12.3 10.1 9.9 9.2 7.6 7.5 8.2 6.7 – 6.3 6.5 13.2 13.4 13.0 15.5 16.0 15.0 11.2 11.3 11.7
Std Deviation (1e-4) 3.5 3.2 2.0 2.2 1.9 1.9 1.9 2.0 1.9 – 1.8 1.6 3.1 2.9 2.8 4.6 4.9 4.7 2.7 2.3 2.1
Intensity Change (Log2) – – – -0.03 -0.13 -0.41 -0.43 -0.3 -0.59 – -0.69 -0.63 0.39 0.41 0.37 0.62 0.66 0.57 0.15 0.16 0.21


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 1.4 – 1.0 0.7 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm -2.8 0.8 -2.2 -3.3 -4.2 -5.4 – -5.6 -5.7 -9.3 -6.8 -5.6 1.0 0.2 0.5
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Endosome 0 1.4 1.6 0 0 0 – 0 0 0 1.7 2.6 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0 0 4.3 3.5 2.9 7.5 – 8.1 5.2 0 0 0 0 0 0
Nucleus 2.2 -2.6 -1.5 0.4 0.1 0.1 – 0.5 1.6 7.4 1.4 -1.2 -1.8 -1.3 -1.7
Nuclear Periphery 0 0 3.1 3.1 3.5 3.7 – 0 0 0 0 0 0 0 0
Nucleolus 2.7 3.7 5.5 5.3 5.2 6.9 – 6.9 6.6 3.9 6.9 6.9 1.0 1.5 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vacuole -2.7 -1.1 -3.2 -4.0 -2.5 -4.7 – -3.9 -3.8 -2.0 -1.1 0.9 0.3 0.6 1.6


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mcm7-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available