Standard name
Human Ortholog
Description Subunit of the HIR complex; HIR is a nucleosome assembly complex involved in regulation of histone gene transcription; mutants display synthetic defects with subunits of FACT, a complex that allows passage of RNA Pol II through nucleosomes

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0.06 0.05 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.07 0.05 0 0.07 0.08 0.07 0.06 0 0 0 0 0.07 0.15 0.16 0.05 0 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.05 0.64 0.6 0.53 0.66 0.73 0.63 0.68 0.75 0.7 0 0 0 0.14 0.13 0.07 0 0 0 0 0 0
Nucleus 0.84 0.81 0.79 0.69 0.66 0.63 0.69 0.68 0.67 0.64 0.67 0.89 0.81 0.78 0.89 0.9 0.87 0.65 0.71 0.66 0.67 0.61 0.56
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.07 0.13 0.09 0.06 0 0 0.08 0.09 0.12 0.1 0.13 0 0 0 0 0 0 0.11 0.1 0.14 0.12 0.15 0.11
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0.06 0 0 0 0 0.06
Vac/Vac Membrane 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0.08 0.08 0.13
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 1 2 4 7 8 17 6 9 8 0 0 0 0 1 2 0 3 1 2 5 4
Bud Neck 0 0 0 1 0 1 0 0 2 0 1 2 0 0 0 0 0 0 0 1 0 4 2
Bud Site 0 0 1 0 5 5 19 11 41 27 23 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0
Cytoplasm 4 4 3 17 28 27 36 34 23 10 14 21 59 71 19 11 22 0 1 2 1 2 5
Endoplasmic Reticulum 0 0 0 0 0 1 1 1 0 0 0 3 1 2 4 10 6 0 0 0 1 0 0
Endosome 3 1 0 1 1 0 0 0 0 0 0 4 7 8 4 2 4 6 2 2 3 9 13
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 0 1
Mitochondria 1 4 77 141 195 244 423 516 447 403 463 4 7 3 50 47 27 2 0 2 0 10 13
Nucleus 46 64 95 162 240 235 397 560 444 344 446 285 330 344 318 322 324 62 72 80 75 192 203
Nuclear Periphery 0 1 0 7 4 2 6 8 12 11 9 0 0 4 7 4 1 0 0 0 0 2 1
Nucleolus 4 10 11 14 9 7 44 75 77 56 83 6 2 13 10 8 13 10 9 17 13 47 40
Peroxisomes 0 0 0 0 1 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0
SpindlePole 1 1 3 4 11 9 5 12 13 4 9 12 11 22 5 10 15 5 3 5 2 9 21
Vac/Vac Membrane 0 6 0 1 6 3 3 9 3 4 2 3 2 2 6 12 8 3 5 7 9 26 46
Unique Cell Count 55 79 120 236 365 371 578 823 661 538 662 321 405 440 358 358 371 95 102 123 112 315 366
Labelled Cell Count 59 91 191 350 504 541 942 1243 1068 868 1058 340 422 472 423 427 423 95 102 123 112 315 366


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.0 5.7 3.6 3.7 3.7 3.5 3.1 3.4 3.2 3.2 3.4 6.0 5.9 5.8 6.2 6.2 6.3 6.4 6.7 6.6
Std Deviation (1e-4) 0.7 1.5 0.5 1.1 1.3 1.0 1.0 1.1 0.9 1.0 1.0 1.2 1.4 1.4 1.1 1.0 1.1 1.6 1.5 1.5
Intensity Change (Log2) 0.04 0.04 -0.06 -0.2 -0.06 -0.16 -0.16 -0.06 0.73 0.71 0.69 0.79 0.78 0.82 0.84 0.9 0.87


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.8 2.0 1.9 1.6 0 0 0 0 1.7 3.6 3.9 1.3 0 1.5
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 -15.2 -16.4 -17.6 -10.8 -11.0 -13.3
Nucleus -2.1 -2.8 -3.2 -2.3 -2.5 -2.6 -3.2 -2.6 2.6 0.6 -0.2 2.7 3.1 2.2
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 -3.0 -2.9 0 -2.5
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 1.2 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.225 1.136 0.892 0.3282 0.738 0.9481 1.2339 1.9787 1.7916 1.1815 1.1201 1.7225 -0.488 -0.4034 -0.6298 0.1452 -0.7065 -0.3487
Actin 0.0087 0 0.0019 0.0001 0.0004 0.0001 0.0008 0.0001 0.0002 0 0 0.0006 0.0135 0 0.0042 0.0974 0.0002 0.0002
Bud 0.0042 0 0.0005 0 0 0 0.0001 0 0 0 0 0 0.0002 0.0002 0.0002 0.0002 0.0001 0.0001
Bud Neck 0.001 0.0001 0.0002 0.0005 0.001 0.0019 0.001 0.0003 0.0002 0.0001 0.0008 0.0017 0.0036 0.0003 0.0005 0.0019 0.002 0.0018
Bud Periphery 0.001 0 0.0006 0 0.0001 0.0001 0.0002 0 0.0001 0 0 0 0.0006 0.0002 0.0006 0.0003 0.0002 0.0002
Bud Site 0.0023 0.0001 0.0012 0 0.0002 0.0001 0.0002 0.0001 0.0001 0 0.0001 0.0001 0.0025 0.0011 0.0005 0.008 0.0001 0.0003
Cell Periphery 0.0001 0 0.0001 0 0 0 0.0001 0 0 0 0 0 0.0001 0.0001 0.0001 0.0001 0 0.0001
Cytoplasm 0.0032 0.0054 0.0005 0.0015 0.0014 0.0003 0.0039 0.0022 0.0009 0.0001 0.0001 0.0008 0.0088 0.0011 0.0063 0.0015 0.0025 0.0009
Cytoplasmic Foci 0.0027 0.0001 0.0008 0 0.0027 0 0.0004 0.0001 0.0001 0 0 0.0004 0.0052 0 0.0021 0.052 0.0001 0.0004
Eisosomes 0.0004 0 0.0001 0 0 0 0 0 0 0 0 0 0.0002 0 0 0.0008 0 0
Endoplasmic Reticulum 0.0029 0.0001 0.0004 0 0.0003 0 0.0006 0.0002 0.0004 0 0 0.0001 0.0008 0 0.0005 0.0002 0.0001 0.0004
Endosome 0.0092 0.0001 0.0033 0.0001 0.0039 0.0001 0.0022 0.0003 0.0023 0 0 0.001 0.0051 0 0.0023 0.0042 0.0003 0.0032
Golgi 0.0025 0 0.0011 0 0.0003 0 0.0003 0 0.0002 0 0 0.0005 0.004 0 0.001 0.0052 0 0.0007
Lipid Particles 0.0035 0 0.0013 0 0.0021 0 0.0017 0.0001 0.001 0 0 0.0025 0.0075 0 0.0028 0.007 0.0001 0.0074
Mitochondria 0.0056 0.0001 0.006 0.0001 0.0007 0.0001 0.0013 0.0002 0.0016 0.0001 0.0002 0.0003 0.002 0.0003 0.0102 0.0027 0.0017 0.0011
None 0.0065 0.0018 0.0006 0.006 0.0017 0.0003 0.0069 0.0171 0.0027 0 0.0001 0.0063 0.0126 0.0005 0.0108 0.0011 0.0012 0.0005
Nuclear Periphery 0.0083 0.0117 0.0122 0.0031 0.017 0.0018 0.0167 0.0201 0.0116 0.0024 0.0018 0.0029 0.0208 0.0042 0.0364 0.0015 0.0122 0.0083
Nucleolus 0.0382 0.0354 0.038 0.0523 0.1055 0.0904 0.0389 0.0481 0.0383 0.0376 0.1455 0.1006 0.0523 0.0394 0.0433 0.0132 0.1731 0.0774
Nucleus 0.8857 0.9259 0.91 0.9141 0.8177 0.8864 0.9116 0.8878 0.9333 0.9585 0.8445 0.8645 0.8287 0.9481 0.8497 0.7611 0.7837 0.8728
Peroxisomes 0.0016 0 0.0025 0 0.0008 0 0.0002 0 0.0001 0 0 0.0007 0.0046 0 0.0085 0.0115 0 0.0003
Punctate Nuclear 0.011 0.0189 0.0181 0.0221 0.0433 0.0181 0.0118 0.0228 0.0059 0.0009 0.0069 0.017 0.0253 0.0042 0.0149 0.0298 0.0216 0.0224
Vacuole 0.0008 0.0001 0.0003 0.0001 0.0004 0.0001 0.0007 0.0002 0.0004 0.0001 0 0.0001 0.0009 0.0001 0.0015 0.0001 0.0003 0.0011
Vacuole Periphery 0.0005 0.0001 0.0002 0 0.0003 0 0.0005 0.0002 0.0004 0.0001 0 0.0001 0.0004 0.0001 0.0036 0.0001 0.0004 0.0007

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8.2256 14.7064 8.5743 12.3782 11.4266 10.5699 9.9573 13.7995 10.6645 13.6504
Translational Efficiency 3.3922 1.347 2.1016 1.4769 1.8964 2.3016 1.6742 1.2561 1.5434 1.6055

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1969 1625 2635 1461 1287 1145 1582 1716 3256 2770 4217 3177

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 700.25 722.29 911.11 955.77 700.72 762.04 931.79 971.51 700.44 738.72 918.87 964.27
Standard Deviation 87.84 131.23 119.09 133.86 94.30 112.87 121.91 136.81 90.45 125.51 120.57 135.69
Intensity Change Log 2 0.044708 0.379755 0.448793 0.121029 0.411167 0.471391 0.083386 0.395552 0.460140

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000106 0.000395 0.000765 0.001400 0.000287 0.000464 0.000977 0.001147 0.000178 0.000424 0.000845 0.001264
Bud Neck 0.004405 0.009213 0.029781 0.024844 0.003278 0.010379 0.017105 0.027036 0.003960 0.009695 0.025025 0.026028
Bud Site 0.001065 0.002789 0.002377 0.005803 0.000785 0.003022 0.002286 0.005587 0.000954 0.002885 0.002343 0.005686
Cell Periphery 0.000181 0.000225 0.000113 0.000114 0.000140 0.000160 0.000107 0.000101 0.000165 0.000198 0.000111 0.000107
Cytoplasm 0.251908* 0.176496* 0.007209 0.013597 0.085644 0.100406* 0.008799 0.010981 0.186189* 0.145044* 0.007806 0.012184
Cytoplasmic Foci 0.036381 0.038682 0.000256 0.000736 0.018791 0.023759 0.000417 0.000896 0.029428 0.032514 0.000317 0.000822
Eisosomes 0.000052 0.000051 0.000031 0.000036 0.000048 0.000054 0.000034 0.000034 0.000050 0.000052 0.000032 0.000035
Endoplasmic Reticulum 0.001023 0.002771 0.003232 0.005819 0.001985 0.002888 0.004850 0.004686 0.001403 0.002820 0.003839 0.005207
Endosome 0.001779 0.002716 0.000637 0.001615 0.001172 0.002963 0.000707 0.001568 0.001539 0.002818 0.000663 0.001590
Golgi 0.000376 0.000876 0.000204 0.000702 0.000194 0.000797 0.000232 0.001530 0.000304 0.000844 0.000215 0.001149
Lipid Particles 0.004698 0.004523 0.000147 0.000280 0.003108 0.004298 0.000273 0.000258 0.004070 0.004430 0.000194 0.000268
Mitochondria 0.002193 0.004111 0.002140 0.004662 0.001849 0.004692 0.001749 0.003731 0.002057 0.004351 0.001994 0.004159
Mitotic Spindle 0.001452 0.007174 0.009194 0.034268 0.001605 0.009538 0.009715 0.039129 0.001512 0.008151 0.009390 0.036893
None 0.011145 0.022293 0.005075 0.008712 0.015708 0.012798 0.007115 0.005200 0.012949 0.018368 0.005840 0.006815
Nuclear Periphery 0.001945 0.001804 0.000930 0.001675 0.001694 0.002662 0.000873 0.002005 0.001846 0.002159 0.000909 0.001853
Nuclear Periphery Foci 0.001447 0.002249 0.000329 0.001271 0.002875 0.001391 0.000644 0.002121 0.002012 0.001894 0.000447 0.001730
Nucleolus 0.017857 0.031060 0.038760 0.046229 0.045126 0.041664 0.041128 0.064023 0.028635 0.035443 0.039648 0.055840
Nucleus 0.654692* 0.680290* 0.896903* 0.838426* 0.810966* 0.765698* 0.899543* 0.824506* 0.716463* 0.715594* 0.897894* 0.830907*
Peroxisomes 0.000982 0.002699 0.000445 0.000549 0.001003 0.004633 0.000524 0.000580 0.000990 0.003499 0.000475 0.000566
Vacuole 0.006056 0.009046 0.001095 0.007191 0.003483 0.006860 0.002438 0.003218 0.005039 0.008143 0.001599 0.005045
Vacuole Periphery 0.000258 0.000535 0.000376 0.002070 0.000259 0.000872 0.000482 0.001664 0.000258 0.000675 0.000416 0.001850

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -10.07 -24.68 -22.98 -17.44 -9.52 -3.08 -17.45 -20.01 -11.88 -2.74 -8.44 -29.70 -31.17* -21.42 -9.51
Bud Neck -5.93 -25.66 -18.16 -13.98 1.93 -7.16 -18.37 -24.12 -15.68 -7.58 -9.06 -31.27 -29.53 -21.48 -2.49
Bud Site -4.44 -8.15 -6.61 -3.71 -3.79 -3.97 -6.16 -6.94 -2.32 -2.88 -5.88 -10.21 -9.43 -4.33 -4.82
Cell Periphery -1.56 5.03 4.01 4.89 -1.98 -1.13 3.60 4.07 5.07 0.18 -1.84 6.12 5.69 6.71 -1.12
Cytoplasm 8.41 37.00 35.16 24.82 -4.38 -1.90 15.59 14.72 15.05 -2.52 6.47 38.22 36.56 28.86 -5.05
Cytoplasmic Foci -0.28 19.18 18.95 14.50 -1.95 -1.44 10.29 10.13 10.16 -1.57 -0.89 21.57 21.28 17.65 -2.69
Eisosomes -0.04 8.14 3.85 3.85 -4.33 -0.88 5.28 4.39 4.98 -1.23 -0.64 9.70 5.84 6.27 -4.43
Endoplasmic Reticulum -11.00 -21.33 -19.40 -10.67 -8.53 -4.63 -7.40 -14.99 -8.07 -0.89 -11.27 -16.39 -25.61 -13.55 -6.69
Endosome -2.66 6.37 2.03 4.30 -2.92 -4.79 3.78 0.82 5.06 -2.42 -5.04 7.43 2.33 6.70 -3.93
Golgi -1.92 0.90 -0.98 1.16 -2.01 -2.59 -2.07 -2.07 -0.35 -1.84 -2.96 0.47 -2.12 0.29 -2.42
Lipid Particles 0.96 11.80 11.31 8.80 -4.77 -1.36 9.14 8.80 7.95 -1.86 0.06 14.77 14.15 11.81 -4.67
Mitochondria -2.34 -0.52 -3.27 -0.56 -3.96 -2.45 -0.48 -3.55 0.86 -5.16 -3.36 -0.70 -4.41 0.37 -5.76
Mitotic Spindle -4.50 -8.10 -10.99 -9.06 -7.20 -4.40 -5.91 -12.91 -10.04 -8.92 -6.30 -10.02 -16.98 -13.73 -11.57
None -6.08 6.94 3.73 8.84 -3.04 1.96 6.99 7.66 5.09 0.38 -3.66 9.88 8.33 10.52 -2.26
Nuclear Periphery 0.09 3.71 -3.16 -3.62 -7.76 -2.64 3.43 -4.76 -0.29 -9.26 -2.05 4.98 -5.50 -2.53 -11.96
Nuclear Periphery Foci -1.74 3.34 -0.34 1.43 -3.92 2.33 3.40 0.15 -2.94 -4.51 0.39 4.74 -0.47 -0.99 -6.01
Nucleolus -5.21 -13.25 -11.75 -7.65 -1.93 1.84 -1.59 -8.23 -9.72 -6.71 -2.44 -10.60 -15.34 -12.95 -6.65
Nucleus -2.20 -25.91 -14.23 -11.21 9.11 3.80 -8.50 3.59 -0.75 13.22 0.11 -25.64 -9.48 -9.01 16.05
Peroxisomes -3.86 4.36 4.36 5.20 -1.36 -3.39 3.73 3.71 4.01 -0.32 -4.87 5.70 5.67 6.04 -1.43
Vacuole -3.47 9.58 -2.77 0.11 -6.42 -3.66 1.63 -1.03 2.96 -2.78 -4.95 7.93 -2.45 2.25 -7.26
Vacuole Periphery -3.02 -0.35 -2.06 -1.39 -1.90 -2.06 -1.11 -2.41 -0.11 -1.62 -3.09 -0.93 -3.11 -1.24 -2.59