Standard name
Human Ortholog
Description RNA binding protein; has similarity to mammalian heterogeneous nuclear RNP K protein, involved in the regulation of telomere position effect and telomere length; relative distribution to the nucleus increases upon DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.81 0.95 0.85 0.63 0.4 0.28 0.2 0.19 0.17 0.14 0.14 0.15 0.98 0.86 0.85 0.65 0.67 0.7 0.7 0.87 0.79 0.89 0.86 0.83
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.17 0.05 0 0.16 0.11 0.41 0.54 0.39 0.46 0.56 0.68 0.61 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0.05 0.09 0.2 0.24 0.31 0.3 0.29 0.28 0.26 0 0 0 0.05 0 0 0.06 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.14 0 0.22 0.31 0.55 0.38 0.3 0.31 0.28 0.26 0.23 0.27 0 0.21 0.18 0.36 0.4 0.32 0.14 0.06 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 2 0 0 1
Bud 1 0 0 2 2 3 10 9 8 8 5 11 0 0 0 0 0 0 1 0 2 0 2 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 1
Bud Site 0 0 0 0 0 0 1 1 1 4 1 2 0 0 0 0 0 0
Cell Periphery 0 0 0 1 0 0 0 0 2 2 1 1 3 0 0 1 5 5 0 0 0 0 0 0
Cytoplasm 119 90 334 172 104 128 90 104 72 58 48 58 208 214 197 197 214 171 114 90 327 162 183 178
Endoplasmic Reticulum 0 0 2 0 1 2 1 0 0 0 0 0 3 0 0 5 8 4 0 0 10 0 1 2
Endosome 0 0 8 2 19 2 7 14 3 3 1 5 0 5 3 2 11 3 1 0 2 1 0 5
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 5 0 0 4 0 1 1
Mitochondria 25 5 4 43 28 183 242 216 201 233 236 242 0 1 0 7 5 9 2 1 17 2 6 3
Nucleus 1 0 0 13 24 91 106 172 128 120 97 104 3 2 5 15 9 7 9 3 14 6 4 3
Nuclear Periphery 0 0 0 0 0 0 0 0 0 1 2 3 0 0 0 0 0 0 0 0 2 0 0 0
Nucleolus 1 0 2 1 0 0 2 2 3 2 4 0 0 0 0 1 2 0 0 0 1 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0 3 0 3 0 1 5
Vac/Vac Membrane 20 2 85 84 144 172 132 176 122 106 81 108 2 52 41 109 128 78 22 6 17 4 4 4
Unique Cell Count 147 95 393 272 261 450 447 560 433 413 345 396 213 250 231 305 319 244 162 104 417 183 213 214
Labelled Cell Count 167 97 435 318 322 581 591 694 541 537 476 534 219 274 246 339 385 282 162 104 417 183 213 214


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.1 5.2 5.9 5.5 6.4 5.1 5.0 5.2 5.0 4.9 4.4 4.5 6.7 6.6 6.2 8.6 8.6 9.2 6.5 7.2 7.5
Std Deviation (1e-4) 0.7 0.8 1.6 1.4 2.5 1.1 1.1 1.2 1.2 1.5 1.0 0.9 1.0 1.2 1.2 1.9 1.4 2.2 1.1 1.7 1.8
Intensity Change (Log2) -0.12 0.11 -0.22 -0.24 -0.2 -0.25 -0.28 -0.45 -0.42 0.18 0.15 0.06 0.53 0.53 0.64 0.12 0.27 0.33


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -6.5 -12.0 -16.5 -18.8 -20.3 -19.6 -20.1 -19.3 -19.8 4.8 0.2 0.1 -6.3 -5.6 -4.5
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 3.3 0 -0.5 0.5 -1.7 -1.6 0 0 0 0 0 0 1.2 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 7.3 5.6 13.8 16.9 13.5 15.1 17.3 0 0 0 0 0 0 0 0
Nucleus 4.4 6.1 9.4 10.3 12.1 11.7 11.6 11.3 10.9 0 0 0 4.4 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 2.7 8.8 5.2 2.6 3.3 2.2 1.3 0.6 1.8 -6.9 -0.3 -1.2 4.1 5.4 2.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.9781 1.7594 1.6291 1.4131 0.9844 1.3426 0.5831 1.648 1.9039 1.6123 1.8755 1.6131 1.0313 1.9439 1.6368 1.9013 1.864 1.6429
Actin 0.0292 0.0003 0.013 0.0026 0.0013 0.008 0.0042 0.0047 0.021 0.0278 0.0322 0.0098 0.006 0.001 0.0118 0.0177 0.0308 0.001
Bud 0.0005 0.0002 0.0007 0.0002 0.0001 0.0008 0.0009 0.0004 0.0018 0.0064 0.0023 0.0003 0.0003 0.001 0.0026 0.0005 0.0034 0.0024
Bud Neck 0.0035 0.0004 0.0019 0.0006 0.0006 0.0015 0.0056 0.0004 0.0014 0.0013 0.0014 0.0015 0.0011 0.0008 0.0096 0.001 0.0062 0.0063
Bud Periphery 0.0006 0.0001 0.0008 0.0002 0.0001 0.0007 0.0019 0.0002 0.0023 0.0095 0.0014 0.0005 0.0003 0.0004 0.0014 0.0006 0.0027 0.0011
Bud Site 0.0091 0.0012 0.0063 0.0004 0.0004 0.0003 0.0039 0.0038 0.0066 0.0249 0.044 0.0004 0.0068 0.0072 0.0166 0.0008 0.0165 0.0023
Cell Periphery 0.0004 0.0001 0.0001 0.0001 0 0 0.0006 0.0001 0.0002 0.0003 0.0002 0.0001 0.0001 0.0001 0.0002 0.0001 0.0003 0.0001
Cytoplasm 0.6093 0.6534 0.5872 0.8335 0.6467 0.6174 0.7513 0.717 0.6276 0.6325 0.4719 0.7509 0.6837 0.7596 0.7083 0.6836 0.7033 0.7012
Cytoplasmic Foci 0.0193 0.012 0.0238 0.0112 0.0104 0.0186 0.0101 0.0083 0.0181 0.0227 0.0422 0.0094 0.0167 0.0111 0.0141 0.0147 0.0142 0.0114
Eisosomes 0.0008 0 0.0002 0.0001 0 0.0001 0.0001 0 0.0002 0.0001 0.0001 0.0003 0.0001 0 0.0001 0.0002 0.0005 0
Endoplasmic Reticulum 0.0108 0.0053 0.0069 0.0097 0.0035 0.0069 0.0149 0.0054 0.0098 0.0142 0.0026 0.0065 0.0076 0.0038 0.0052 0.0071 0.0072 0.005
Endosome 0.02 0.0097 0.0215 0.0174 0.006 0.0142 0.0099 0.0053 0.0139 0.0185 0.0425 0.0104 0.0184 0.0041 0.0127 0.0321 0.0173 0.0077
Golgi 0.0051 0.0003 0.0123 0.0012 0.0031 0.0014 0.0017 0.0018 0.0089 0.0149 0.0092 0.0052 0.0032 0.0003 0.0035 0.0164 0.0049 0.0003
Lipid Particles 0.0059 0.0005 0.0033 0.0019 0.0155 0.0007 0.0067 0.0006 0.0039 0.0028 0.0153 0.0026 0.004 0.0001 0.0029 0.0256 0.0058 0.0002
Mitochondria 0.0046 0.0004 0.0051 0.001 0.0052 0.0014 0.0022 0.0021 0.0054 0.0052 0.0062 0.0118 0.0045 0.0003 0.0018 0.0178 0.0021 0.0009
None 0.2076 0.1663 0.2121 0.069 0.1803 0.226 0.1336 0.1942 0.1539 0.1429 0.2622 0.1108 0.1817 0.0991 0.0855 0.0682 0.0769 0.1122
Nuclear Periphery 0.0101 0.0085 0.01 0.007 0.0059 0.005 0.0132 0.0041 0.0079 0.004 0.0016 0.0072 0.0101 0.0046 0.0123 0.0061 0.0148 0.0138
Nucleolus 0.0004 0.0005 0.0015 0.0003 0.0003 0.0004 0.0059 0.0003 0.0004 0.0005 0.0004 0.0006 0.0009 0.0004 0.0013 0.0003 0.0015 0.0004
Nucleus 0.0408 0.1298 0.0733 0.03 0.0962 0.0848 0.0186 0.0427 0.0989 0.0538 0.0024 0.0605 0.0396 0.0962 0.0862 0.0807 0.0715 0.1193
Peroxisomes 0.0127 0.0005 0.004 0.0005 0.0193 0.0038 0.0027 0.0009 0.0094 0.0091 0.0533 0.0005 0.0046 0.0003 0.0074 0.0169 0.0026 0.0006
Punctate Nuclear 0.0053 0.0071 0.0129 0.0083 0.0037 0.0057 0.0081 0.0045 0.0055 0.0045 0.0016 0.0076 0.0059 0.0078 0.013 0.0052 0.0142 0.0109
Vacuole 0.0033 0.003 0.0026 0.004 0.001 0.0019 0.003 0.0027 0.0024 0.0034 0.006 0.0025 0.0036 0.0018 0.0026 0.0023 0.0024 0.0021
Vacuole Periphery 0.0007 0.0004 0.0006 0.0009 0.0003 0.0004 0.0006 0.0003 0.0005 0.0007 0.0009 0.0008 0.0009 0.0002 0.0007 0.002 0.0009 0.0009

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.3261 8.736 4.7145 17.2402 16.1261 11.5429 14.6547 14.9042 16.267 20.9239
Translational Efficiency 2.8448 2.3348 3.7672 1.4582 1.7356 3.7783 1.5145 1.3465 1.4777 1.5471

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
168 753 151 1414 1131 1615 1900 1252 1299 2368 2051 2666

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 779.63 795.61 979.39 932.29 695.18 748.23 917.05 976.87 706.10 763.30 921.64 953.23
Standard Deviation 92.94 98.59 116.94 152.12 85.38 98.20 115.50 155.77 90.93 100.77 116.75 155.44
Intensity Change Log 2 0.029272 0.329094 0.257989 0.106095 0.399614 0.490780 0.065994 0.362765 0.372409

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000474 0.000381 0.001098 0.002577 0.000149 0.000369 0.000222 0.000761 0.000191 0.000373 0.000287 0.001724
Bud Neck 0.004517 0.012060 0.003365 0.011086 0.002511 0.015457 0.003225 0.012428 0.002771 0.014377 0.003235 0.011716
Bud Site 0.003724 0.011477 0.004201 0.028029 0.003201 0.010777 0.006505 0.029618 0.003268 0.011000 0.006335 0.028775
Cell Periphery 0.000284 0.000135 0.000338 0.000258 0.000172 0.000134 0.000114 0.000177 0.000186 0.000135 0.000131 0.000220
Cytoplasm 0.441146* 0.483701* 0.209943* 0.385330* 0.602356* 0.469044* 0.479548* 0.394018* 0.581507* 0.473705* 0.459699* 0.389410*
Cytoplasmic Foci 0.161311 0.176456 0.013980 0.013831 0.095462 0.142718 0.012857 0.012016 0.103978 0.153446 0.012940 0.012979
Eisosomes 0.000122 0.000076 0.000125 0.000053 0.000050 0.000081 0.000030 0.000038 0.000059 0.000080 0.000037 0.000046
Endoplasmic Reticulum 0.002970 0.001825 0.018454 0.006983 0.001424 0.001124 0.002793 0.004589 0.001624 0.001347 0.003946 0.005859
Endosome 0.007165 0.013004 0.007184 0.006725 0.003163 0.010793 0.003163 0.005753 0.003680 0.011496 0.003459 0.006269
Golgi 0.001186 0.004081 0.000176 0.004842 0.000440 0.001992 0.000315 0.006184 0.000537 0.002657 0.000305 0.005472
Lipid Particles 0.009330 0.004544 0.004640 0.002127 0.002941 0.005974 0.000971 0.001461 0.003767 0.005519 0.001242 0.001814
Mitochondria 0.011838 0.005133 0.000637 0.004351 0.001455 0.008107 0.000892 0.012217 0.002798 0.007161 0.000873 0.008045
Mitotic Spindle 0.006163 0.007542 0.000693 0.041465 0.001538 0.009673 0.004563 0.060387 0.002136 0.008996 0.004278 0.050351
None 0.002412 0.005482 0.002163 0.006931 0.010904 0.004391 0.006582 0.008044 0.009806 0.004738 0.006257 0.007454
Nuclear Periphery 0.000796 0.000701 0.002694 0.002660 0.000414 0.000874 0.001666 0.002349 0.000464 0.000819 0.001741 0.002514
Nuclear Periphery Foci 0.003849 0.003070 0.006174 0.004943 0.000982 0.000474 0.001378 0.003261 0.001353 0.001300 0.001731 0.004153
Nucleolus 0.001241 0.001433 0.000965 0.000565 0.000874 0.002372 0.000410 0.001176 0.000922 0.002074 0.000451 0.000852
Nucleus 0.297271* 0.207348* 0.572235* 0.355002* 0.250623* 0.245102* 0.411725* 0.347289* 0.256656* 0.233097* 0.423542* 0.351380*
Peroxisomes 0.003102 0.006621 0.000540 0.001101 0.000953 0.007305 0.000510 0.001660 0.001231 0.007088 0.000512 0.001364
Vacuole 0.038882 0.053909 0.150090 0.120015 0.019783 0.061162 0.061705 0.093531 0.022253 0.058855 0.068212 0.107578
Vacuole Periphery 0.002217 0.001021 0.000308 0.001125 0.000602 0.002075 0.000827 0.003044 0.000811 0.001740 0.000788 0.002026

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.42 -3.06 -2.51 -3.84 -0.10 -2.15 -2.50 -11.56 -4.39 -10.06 -2.20 -2.75 -6.40 -4.94 -5.61
Bud Neck -3.80 0.15 -5.50 0.19 -6.75 -10.31 -3.29 -8.11 2.83 -6.48 -11.10 -3.00 -11.32 2.51 -9.28
Bud Site -3.38 -1.33 -6.47 -1.87 -6.08 -5.12 -2.26 -6.86 -2.64 -5.35 -6.25 -2.34 -9.86 -3.34 -7.29
Cell Periphery 3.04 -1.39 1.58 -6.48 5.29 3.79 6.89 1.34 -3.08 -7.22 4.88 5.68 0.20 -7.26 -8.50
Cytoplasm -1.74 9.22 7.14 16.96 -5.06 12.97 20.13 30.45 18.20 12.38 11.70 20.30 34.09 25.35 13.62
Cytoplasmic Foci -0.89 11.76 12.05 23.01 1.57 -8.11 21.78 22.49 30.27 2.49 -9.17 24.41 25.17 37.87 2.57
Eisosomes 2.56 -1.10 3.28 2.59 7.38 -7.67 4.78 -1.47 6.83 -7.21 -4.95 4.16 -0.17 6.65 -7.00
Endoplasmic Reticulum 1.24 -8.69 -5.85 -17.38 6.60 1.77 -9.88 -15.64 -17.50 -7.64 1.38 -11.45 -20.78 -25.21 -7.24
Endosome -2.34 0.01 2.25 6.04 4.13 -8.96 1.13 -0.15 8.86 -1.25 -9.81 1.53 0.28 10.41 -1.43
Golgi -2.22 2.43 -0.24 1.90 -1.80 -4.35 2.35 -1.38 0.46 -1.68 -4.62 3.17 -1.78 1.64 -2.44
Lipid Particles 1.93 1.75 2.73 4.60 4.85 -5.18 6.21 2.91 7.77 -8.62 -3.02 5.70 3.45 8.61 -9.45
Mitochondria 1.04 1.75 1.50 1.98 -3.77 -5.17 1.95 -2.03 2.56 -2.69 -3.34 2.29 -0.15 3.75 -3.74
Mitotic Spindle -0.20 2.06 -6.18 -6.43 -9.42 -4.13 -3.01 -10.94 -8.93 -10.07 -4.15 -2.40 -13.73 -10.84 -12.54
None -2.25 1.33 -5.53 -0.24 -6.93 5.11 4.39 1.64 -5.30 -4.25 4.42 4.20 2.40 -3.57 -3.28
Nuclear Periphery 0.67 -12.49 -19.80 -21.74 -6.45 -6.01 -24.45 -20.99 -17.19 -8.58 -5.86 -25.97 -31.65 -27.14 -13.43
Nuclear Periphery Foci 0.31 -1.36 -1.19 -2.55 0.42 4.39 -2.95 -7.88 -8.85 -6.49 0.07 -1.83 -8.49 -7.83 -8.10
Nucleolus -0.49 1.28 0.10 0.74 -1.65 -3.73 5.26 -3.27 1.98 -5.60 -4.01 5.49 -3.03 2.33 -6.50
Nucleus 3.75 -8.00 -4.12 -16.60 6.56 0.58 -21.71 -14.23 -15.75 5.13 2.76 -22.64 -16.17 -22.44 8.07
Peroxisomes -2.23 3.02 3.01 6.15 0.10 -7.48 2.50 0.73 7.62 -1.36 -8.57 3.70 2.94 9.79 -1.13
Vacuole -2.59 -8.97 -21.57 -22.39 -1.57 -13.74 -19.35 -24.15 -16.56 -10.88 -14.60 -20.63 -37.05 -27.60 -16.37
Vacuole Periphery 1.34 2.11 1.98 3.73 -1.17 -3.86 0.35 -0.85 2.77 -0.92 -3.15 1.24 1.04 3.90 -0.47