Standard name
Human Ortholog
Description Putative transporter, member of the sugar porter family; green fluorescent protein (GFP)-fusion protein localizes to the vacuolar membrane; YBR241C is not an essential gene; YBR241C has a paralog, VPS73, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.06 0.13 0.12 0.43 0.5 0.46 0.36 0.42 0.45 0.47 0.54 0.46 0.18 0.24 0.11 0.25 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0.11 0.06 0.08 0.08 0.1 0.08 0.05 0.05 0.05 0 0 0 0.12 0 0 0 0 0 0
Endosome 0.08 0 0.17 0.11 0.09 0 0 0 0 0 0 0 0 0.06 0.05 0 0.08 0 0.12 0.05 0.08 0.1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.06 0 0.11 0.17 0.13 0.27 0.27 0.27 0.25 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.91 0.87 0.82 0.39 0.49 0.41 0.45 0.39 0.29 0.3 0.26 0.33 0.81 0.79 0.91 0.67 0.83 0.86 0.77 0.83 0.81 0.8
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 1 0 3 10 3 8 2 2 2 1 0 0 0 0 0 0 1 0
Bud 0 0 0 0 0 0 5 1 4 2 3 4 0 1 0 0 0 1 3 2 1 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 1 1 4 11 16 14 10 11 0 0 0 0 0 0 0 0 0 0
Cytoplasm 7 38 51 99 182 223 240 325 196 205 267 223 33 30 17 6 0 6 1 1 1 3
Endoplasmic Reticulum 2 3 6 26 22 39 53 76 37 20 23 22 4 2 3 3 0 1 3 0 2 1
Endosome 10 5 73 25 31 18 27 21 7 6 3 10 4 8 8 0 11 13 54 7 21 23
Golgi 1 1 4 4 7 12 28 29 8 5 5 9 0 2 2 1 0 2 2 0 1 0
Mitochondria 4 7 13 13 6 51 111 102 118 116 134 122 0 0 0 1 3 3 4 1 2 3
Nucleus 2 10 5 1 2 5 5 7 3 2 5 0 9 5 4 0 2 3 2 5 5 7
Nuclear Periphery 3 4 8 3 1 2 8 27 9 4 2 5 3 0 2 0 3 1 26 5 6 4
Nucleolus 3 2 13 1 0 1 4 3 1 3 1 1 0 1 1 0 0 1 1 1 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 4 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0 0 1
Vac/Vac Membrane 108 250 354 91 177 200 298 306 127 131 131 163 149 100 144 16 111 264 354 130 208 192
Unique Cell Count 119 289 434 231 362 484 669 781 437 434 499 487 184 127 159 24 135 307 460 156 257 242
Labelled Cell Count 140 320 531 264 430 552 786 918 529 516 587 572 204 150 181 27 135 307 460 156 257 242


Vacuole Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.9 8.7 7.7 7.8 9.3 8.9 9.2 10.0 9.4 9.4 9.5 10.1 9.1 10.1 11.3 10.0 7.8 9.1 10.0
Std Deviation (1e-4) 1.7 1.8 1.7 1.8 2.3 2.3 2.3 2.6 2.8 2.9 3.0 3.3 2.3 2.5 3.3 3.0 1.7 1.9 2.3
Intensity Change (Log2) 0.01 0.26 0.21 0.25 0.37 0.28 0.28 0.29 0.38 0.23 0.38 0.54 0.36 0.0 0.23 0.37


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 4.0 3.8 0 3.1 0 0 0 0
Cytoplasm 9.1 11.9 11.3 8.9 10.8 10.8 11.5 13.4 11.3 2.1 3.3 -0.4 0
Endoplasmic Reticulum 5.7 3.6 4.7 4.7 5.6 4.8 2.8 2.8 2.8 0 0 0 0
Endosome -2.1 -3.4 -6.6 -7.2 -8.8 -7.8 -7.9 -9.0 -7.8 -5.0 -3.0 -3.7 0
Golgi 0 1.2 1.8 3.2 2.9 1.2 0 0 1.2 0 0 0 0
Mitochondria 1.7 -1.2 4.5 7.0 5.7 9.9 9.8 10.0 9.4 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 -0.9 1.6 0.2 -1.2 0 -1.0 0 0 0 0
Nucleolus 0 -3.3 -3.4 -3.2 -3.8 -3.2 -2.5 -3.5 -3.5 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -11.0 -9.7 -12.4 -12.2 -14.2 -15.6 -15.2 -16.9 -14.7 -0.2 -0.7 2.6 -1.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration
Actin 0.0057 0 0.0012 0.0383 0.0001 0.0012 0.0081 0 0.0047 0 0 0.0001
Bud 0.0005 0.0008 0.0033 0.0006 0.0004 0.0017 0.0008 0.0029 0.001 0.0002 0.0004 0.0003
Bud Neck 0.0038 0.0005 0.0008 0.0006 0.0004 0.0012 0.001 0.0007 0.0021 0.0004 0.0003 0.0006
Bud Periphery 0.001 0.0011 0.0075 0.0007 0.0006 0.0027 0.0018 0.0037 0.0019 0.0003 0.0008 0.0006
Bud Site 0.0014 0.0024 0.0044 0.0018 0.0002 0.0011 0.0027 0.0061 0.0027 0.0009 0.0005 0.0005
Cell Periphery 0.0007 0.0008 0.0049 0.0003 0.0006 0.0005 0.0011 0.0008 0.0012 0.0004 0.0005 0.0007
Cytoplasm 0.021 0.0435 0.0325 0.0219 0.0139 0.0292 0.0192 0.0175 0.0209 0.006 0.0178 0.019
Cytoplasmic Foci 0.0127 0.0066 0.0085 0.0099 0.007 0.0068 0.012 0.0083 0.0163 0.0109 0.0087 0.0088
Eisosomes 0.0001 0 0.0001 0.0002 0 0 0.0008 0 0 0 0 0
Endoplasmic Reticulum 0.0065 0.0052 0.0056 0.0026 0.0016 0.0049 0.0055 0.0097 0.0043 0.001 0.0023 0.0032
Endosome 0.1943 0.1378 0.1646 0.1106 0.1926 0.1563 0.2153 0.1456 0.249 0.3025 0.2013 0.2123
Golgi 0.01 0.0019 0.0024 0.0114 0.0032 0.0043 0.0077 0.0022 0.0083 0.0041 0.0081 0.0039
Lipid Particles 0.0091 0.0009 0.0036 0.0092 0.0013 0.0052 0.0093 0.0018 0.0037 0.0037 0.0054 0.0039
Mitochondria 0.0116 0.0025 0.0062 0.0022 0.0042 0.0052 0.0182 0.0048 0.0117 0.0024 0.0061 0.0106
None 0.0127 0.0025 0.0121 0.0136 0.0004 0.0009 0.0152 0.0026 0.0088 0.0005 0.0401 0.0126
Nuclear Periphery 0.0245 0.0155 0.012 0.011 0.0059 0.0141 0.0097 0.0127 0.0139 0.0038 0.004 0.0185
Nucleolus 0.0031 0.0017 0.0019 0.0011 0.0006 0.0015 0.002 0.0018 0.0033 0.0016 0.0007 0.0013
Nucleus 0.0105 0.0136 0.0056 0.0036 0.0019 0.0135 0.0107 0.0046 0.01 0.0018 0.0027 0.0043
Peroxisomes 0.0068 0.0001 0.0005 0.0048 0.0002 0.0061 0.0088 0.0006 0.0019 0.0005 0.0003 0.0004
Punctate Nuclear 0.0028 0.0002 0.0002 0.0052 0.0002 0.001 0.0009 0.0002 0.0007 0.0002 0.0004 0.0002
Vacuole 0.4708 0.5879 0.5252 0.5727 0.506 0.4983 0.4399 0.5305 0.4265 0.4786 0.3914 0.4089
Vacuole Periphery 0.1905 0.1747 0.1969 0.1775 0.2588 0.2442 0.2093 0.2429 0.207 0.1802 0.3081 0.2891

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 29.3339 18.8265 9.9786 8.1089 8.7768 20.2968 19.8853 13.0771 15.6881 18.0346
Translational Efficiency 0.5224 0.398 0.5743 0.8315 1.2283 0.9191 0.6416 0.8488 0.7656 0.7148

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
155 1555 279 1142 1664 2178 2740 1863 1819 3733 3019 3005

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 954.16 1038.85 1339.24 1031.52 790.11 977.14 958.51 990.74 804.09 1002.85 993.70 1006.24
Standard Deviation 166.62 200.90 282.55 200.82 114.22 152.74 145.68 156.93 128.06 177.06 196.97 176.03
Intensity Change Log 2 0.122684 0.489111 0.112469 0.306512 0.278740 0.326453 0.208865 0.397598 0.213345

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000379 0.000028 0.000130 0.000092 0.000027 0.000017 0.000015 0.000043 0.000057 0.000021 0.000025 0.000061
Bud Neck 0.005961 0.001065 0.010320 0.005816 0.002848 0.001263 0.005086 0.004685 0.003113 0.001181 0.005570 0.005115
Bud Site 0.008770 0.004678 0.005229 0.025190 0.009182 0.001907 0.010341 0.017187 0.009147 0.003061 0.009869 0.020228
Cell Periphery 0.000079 0.000112 0.000314 0.000132 0.000161 0.000124 0.000068 0.000156 0.000154 0.000119 0.000090 0.000147
Cytoplasm 0.030765 0.009998 0.003073 0.049016 0.043547 0.002209 0.040873 0.037658 0.042458 0.005454 0.037380 0.041974
Cytoplasmic Foci 0.019117 0.011181 0.005174 0.018415 0.035597 0.004986 0.008072 0.014938 0.034193 0.007567 0.007804 0.016259
Eisosomes 0.000020 0.000003 0.000049 0.000008 0.000012 0.000005 0.000005 0.000007 0.000013 0.000005 0.000009 0.000007
Endoplasmic Reticulum 0.002541 0.001805 0.002562 0.002347 0.008678 0.003143 0.001091 0.001859 0.008156 0.002586 0.001227 0.002045
Endosome 0.114840 0.037150 0.030548 0.051469 0.063217 0.034299 0.031978 0.065440 0.067616 0.035486 0.031846 0.060131
Golgi 0.010990 0.006158 0.003673 0.007531 0.002321 0.002980 0.003303 0.005523 0.003060 0.004304 0.003337 0.006286
Lipid Particles 0.001740 0.000405 0.002099 0.000680 0.002134 0.000593 0.000233 0.000672 0.002100 0.000515 0.000406 0.000675
Mitochondria 0.003803 0.001719 0.001394 0.002313 0.004119 0.003712 0.001774 0.002847 0.004092 0.002882 0.001739 0.002644
Mitotic Spindle 0.005022 0.001987 0.001443 0.009825 0.000704 0.000776 0.002281 0.003996 0.001072 0.001281 0.002203 0.006211
None 0.000073 0.000451 0.000263 0.000344 0.000371 0.000165 0.000273 0.000280 0.000345 0.000284 0.000272 0.000304
Nuclear Periphery 0.002882 0.000994 0.004909 0.001584 0.000288 0.001321 0.000869 0.001533 0.000509 0.001185 0.001243 0.001552
Nuclear Periphery Foci 0.004051 0.001054 0.006826 0.002116 0.000375 0.001114 0.000396 0.000753 0.000688 0.001089 0.000990 0.001271
Nucleolus 0.002162 0.000686 0.015514 0.001534 0.000594 0.001609 0.001888 0.001210 0.000727 0.001225 0.003147 0.001333
Nucleus 0.016908 0.001581 0.036165 0.006084 0.002727 0.002802 0.008962 0.004734 0.003936 0.002294 0.011476 0.005247
Peroxisomes 0.001657 0.000282 0.002373 0.000983 0.000376 0.000407 0.000382 0.000648 0.000485 0.000355 0.000566 0.000775
Vacuole 0.651816* 0.367539* 0.429996* 0.624526* 0.569515* 0.494518* 0.630349* 0.632263* 0.576528* 0.441624* 0.611833* 0.629323*
Vacuole Periphery 0.116424* 0.551124* 0.437946* 0.189993* 0.253207* 0.442048* 0.251763* 0.203569* 0.241551* 0.487484* 0.268969* 0.198409*

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.57 1.14 1.22 -3.03 0.56 1.66 1.94 -2.35 -4.28 -4.81 1.77 1.50 -0.45 -4.31 -3.79
Bud Neck 4.37 -2.14 -0.15 -6.96 2.24 3.19 -4.63 -3.56 -7.23 0.91 4.51 -5.12 -4.30 -10.10 0.94
Bud Site 1.96 1.57 -4.98 -7.96 -7.35 7.00 -1.30 -4.82 -10.90 -4.39 6.09 -0.96 -7.14 -13.05 -7.27
Cell Periphery -1.21 -2.57 -2.07 -0.89 2.05 1.89 6.23 0.12 -0.89 -2.57 2.18 3.95 0.30 -1.15 -2.33
Cytoplasm 2.48 3.10 -2.82 -11.30 -12.42 15.20 -0.57 -0.14 -14.67 0.41 14.04 0.24 -2.24 -18.18 -2.66
Cytoplasmic Foci 2.53 4.30 0.33 -4.02 -7.02 15.97 14.59 9.99 -9.11 -6.64 14.96 15.07 9.62 -9.02 -9.32
Eisosomes 4.06 -2.66 3.04 -7.40 4.31 3.21 3.63 2.30 -2.66 -5.28 4.32 1.98 2.81 -5.81 1.28
Endoplasmic Reticulum 0.92 -0.05 0.35 -1.08 0.69 7.98 11.48 9.99 4.42 -3.60 8.86 11.33 9.70 2.28 -3.86
Endosome 6.73 6.86 5.64 -3.53 -3.48 9.20 12.13 -0.33 -9.38 -12.23 11.18 13.74 2.97 -9.69 -12.97
Golgi 1.21 1.85 0.95 -0.95 -3.30 -1.23 -3.34 -5.63 -3.39 -3.93 -2.26 -0.58 -5.27 -2.96 -5.89
Lipid Particles 1.79 -0.33 1.37 -3.05 2.54 4.82 6.12 3.98 -0.79 -2.94 5.40 5.71 4.43 -1.84 -2.57
Mitochondria 1.28 1.48 0.86 -0.56 -0.92 0.55 4.39 1.97 1.32 -2.22 2.05 4.64 2.36 0.54 -1.85
Mitotic Spindle 1.38 1.58 -1.73 -4.12 -4.35 -0.17 -2.81 -4.01 -4.26 -2.01 -0.35 -2.08 -5.51 -5.68 -4.29
None -2.20 -2.28 -3.03 0.44 -0.68 1.59 0.69 0.57 -1.81 -0.12 0.44 0.54 0.21 -0.38 -0.64
Nuclear Periphery 1.16 -1.20 0.75 -1.65 4.25 -6.06 -4.57 -2.69 -0.46 -1.15 -3.55 -4.08 -3.06 -1.18 -0.64
Nuclear Periphery Foci 1.18 -1.00 0.68 -2.23 2.47 -1.56 -0.39 -2.04 0.69 -2.11 -1.07 -1.20 -2.01 -0.76 -1.01
Nucleolus 2.25 -5.26 0.81 -2.89 5.62 -3.51 -6.27 -2.82 1.35 3.59 -2.24 -7.89 -2.97 -0.70 6.10
Nucleus 3.25 -2.27 1.93 -6.82 3.88 -0.10 -7.45 -2.86 -3.33 4.90 2.19 -7.06 -2.58 -6.89 5.72
Peroxisomes 2.87 -1.06 1.32 -2.75 2.60 -0.31 -0.17 -3.05 -2.35 -2.51 1.56 -0.85 -2.32 -3.67 -1.45
Vacuole 13.62 8.52 1.36 -26.86 -10.63 9.19 -7.97 -7.51 -17.66 -0.23 18.83 -4.72 -6.97 -30.70 -2.65
Vacuole Periphery -29.43* -13.38 -4.44 39.63* 12.20 -23.77* 1.04 7.53 32.37* 7.32 -35.52 -3.16 7.84 49.88* 12.29