Standard name | |
---|---|
Human Ortholog | |
Description | Putative transporter, member of the sugar porter family; green fluorescent protein (GFP)-fusion protein localizes to the vacuolar membrane; YBR241C is not an essential gene; YBR241C has a paralog, VPS73, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 1 | 1 | 0 | 3 | 10 | 3 | 8 | 2 | 2 | 2 | 1 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 1 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 5 | 1 | 4 | 2 | 3 | 4 | 0 | 1 | 0 | 0 | – | – | 0 | 1 | 3 | 2 | 1 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 1 | 1 | 4 | 11 | 16 | 14 | 10 | 11 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 7 | 38 | 51 | 99 | 182 | 223 | 240 | 325 | 196 | 205 | 267 | 223 | 33 | 30 | 17 | 6 | – | – | 0 | 6 | 1 | 1 | 1 | 3 |
Endoplasmic Reticulum | 2 | 3 | 6 | 26 | 22 | 39 | 53 | 76 | 37 | 20 | 23 | 22 | 4 | 2 | 3 | 3 | – | – | 0 | 1 | 3 | 0 | 2 | 1 |
Endosome | 10 | 5 | 73 | 25 | 31 | 18 | 27 | 21 | 7 | 6 | 3 | 10 | 4 | 8 | 8 | 0 | – | – | 11 | 13 | 54 | 7 | 21 | 23 |
Golgi | 1 | 1 | 4 | 4 | 7 | 12 | 28 | 29 | 8 | 5 | 5 | 9 | 0 | 2 | 2 | 1 | – | – | 0 | 2 | 2 | 0 | 1 | 0 |
Mitochondria | 4 | 7 | 13 | 13 | 6 | 51 | 111 | 102 | 118 | 116 | 134 | 122 | 0 | 0 | 0 | 1 | – | – | 3 | 3 | 4 | 1 | 2 | 3 |
Nucleus | 2 | 10 | 5 | 1 | 2 | 5 | 5 | 7 | 3 | 2 | 5 | 0 | 9 | 5 | 4 | 0 | – | – | 2 | 3 | 2 | 5 | 5 | 7 |
Nuclear Periphery | 3 | 4 | 8 | 3 | 1 | 2 | 8 | 27 | 9 | 4 | 2 | 5 | 3 | 0 | 2 | 0 | – | – | 3 | 1 | 26 | 5 | 6 | 4 |
Nucleolus | 3 | 2 | 13 | 1 | 0 | 1 | 4 | 3 | 1 | 3 | 1 | 1 | 0 | 1 | 1 | 0 | – | – | 0 | 1 | 1 | 1 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 1 | 0 | 0 | 1 |
Vac/Vac Membrane | 108 | 250 | 354 | 91 | 177 | 200 | 298 | 306 | 127 | 131 | 131 | 163 | 149 | 100 | 144 | 16 | – | – | 111 | 264 | 354 | 130 | 208 | 192 |
Unique Cell Count | 119 | 289 | 434 | 231 | 362 | 484 | 669 | 781 | 437 | 434 | 499 | 487 | 184 | 127 | 159 | 24 | 135 | 307 | 460 | 156 | 257 | 242 | ||
Labelled Cell Count | 140 | 320 | 531 | 264 | 430 | 552 | 786 | 918 | 529 | 516 | 587 | 572 | 204 | 150 | 181 | 27 | 135 | 307 | 460 | 156 | 257 | 242 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.9 | 8.7 | 7.7 | 7.8 | 9.3 | 8.9 | 9.2 | 10.0 | 9.4 | 9.4 | 9.5 | 10.1 | 9.1 | 10.1 | 11.3 | 10.0 | – | – | 7.8 | 9.1 | 10.0 |
Std Deviation (1e-4) | 1.7 | 1.8 | 1.7 | 1.8 | 2.3 | 2.3 | 2.3 | 2.6 | 2.8 | 2.9 | 3.0 | 3.3 | 2.3 | 2.5 | 3.3 | 3.0 | – | – | 1.7 | 1.9 | 2.3 |
Intensity Change (Log2) | – | – | – | 0.01 | 0.26 | 0.21 | 0.25 | 0.37 | 0.28 | 0.28 | 0.29 | 0.38 | 0.23 | 0.38 | 0.54 | 0.36 | – | – | 0.0 | 0.23 | 0.37 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 4.0 | 3.8 | 0 | 3.1 | 0 | 0 | 0 | 0 | – | – |
Cytoplasm | 9.1 | 11.9 | 11.3 | 8.9 | 10.8 | 10.8 | 11.5 | 13.4 | 11.3 | 2.1 | 3.3 | -0.4 | 0 | – | – |
Endoplasmic Reticulum | 5.7 | 3.6 | 4.7 | 4.7 | 5.6 | 4.8 | 2.8 | 2.8 | 2.8 | 0 | 0 | 0 | 0 | – | – |
Endosome | -2.1 | -3.4 | -6.6 | -7.2 | -8.8 | -7.8 | -7.9 | -9.0 | -7.8 | -5.0 | -3.0 | -3.7 | 0 | – | – |
Golgi | 0 | 1.2 | 1.8 | 3.2 | 2.9 | 1.2 | 0 | 0 | 1.2 | 0 | 0 | 0 | 0 | – | – |
Mitochondria | 1.7 | -1.2 | 4.5 | 7.0 | 5.7 | 9.9 | 9.8 | 10.0 | 9.4 | 0 | 0 | 0 | 0 | – | – |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
Nuclear Periphery | 0 | 0 | 0 | -0.9 | 1.6 | 0.2 | -1.2 | 0 | -1.0 | 0 | 0 | 0 | 0 | – | – |
Nucleolus | 0 | -3.3 | -3.4 | -3.2 | -3.8 | -3.2 | -2.5 | -3.5 | -3.5 | 0 | 0 | 0 | 0 | – | – |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
Vacuole | -11.0 | -9.7 | -12.4 | -12.2 | -14.2 | -15.6 | -15.2 | -16.9 | -14.7 | -0.2 | -0.7 | 2.6 | -1.8 | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Actin | 0.0057 | 0 | 0.0012 | 0.0383 | 0.0001 | 0.0012 | 0.0081 | 0 | 0.0047 | 0 | 0 | 0.0001 | – | – | – | – | – | – |
Bud | 0.0005 | 0.0008 | 0.0033 | 0.0006 | 0.0004 | 0.0017 | 0.0008 | 0.0029 | 0.001 | 0.0002 | 0.0004 | 0.0003 | – | – | – | – | – | – |
Bud Neck | 0.0038 | 0.0005 | 0.0008 | 0.0006 | 0.0004 | 0.0012 | 0.001 | 0.0007 | 0.0021 | 0.0004 | 0.0003 | 0.0006 | – | – | – | – | – | – |
Bud Periphery | 0.001 | 0.0011 | 0.0075 | 0.0007 | 0.0006 | 0.0027 | 0.0018 | 0.0037 | 0.0019 | 0.0003 | 0.0008 | 0.0006 | – | – | – | – | – | – |
Bud Site | 0.0014 | 0.0024 | 0.0044 | 0.0018 | 0.0002 | 0.0011 | 0.0027 | 0.0061 | 0.0027 | 0.0009 | 0.0005 | 0.0005 | – | – | – | – | – | – |
Cell Periphery | 0.0007 | 0.0008 | 0.0049 | 0.0003 | 0.0006 | 0.0005 | 0.0011 | 0.0008 | 0.0012 | 0.0004 | 0.0005 | 0.0007 | – | – | – | – | – | – |
Cytoplasm | 0.021 | 0.0435 | 0.0325 | 0.0219 | 0.0139 | 0.0292 | 0.0192 | 0.0175 | 0.0209 | 0.006 | 0.0178 | 0.019 | – | – | – | – | – | – |
Cytoplasmic Foci | 0.0127 | 0.0066 | 0.0085 | 0.0099 | 0.007 | 0.0068 | 0.012 | 0.0083 | 0.0163 | 0.0109 | 0.0087 | 0.0088 | – | – | – | – | – | – |
Eisosomes | 0.0001 | 0 | 0.0001 | 0.0002 | 0 | 0 | 0.0008 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Endoplasmic Reticulum | 0.0065 | 0.0052 | 0.0056 | 0.0026 | 0.0016 | 0.0049 | 0.0055 | 0.0097 | 0.0043 | 0.001 | 0.0023 | 0.0032 | – | – | – | – | – | – |
Endosome | 0.1943 | 0.1378 | 0.1646 | 0.1106 | 0.1926 | 0.1563 | 0.2153 | 0.1456 | 0.249 | 0.3025 | 0.2013 | 0.2123 | – | – | – | – | – | – |
Golgi | 0.01 | 0.0019 | 0.0024 | 0.0114 | 0.0032 | 0.0043 | 0.0077 | 0.0022 | 0.0083 | 0.0041 | 0.0081 | 0.0039 | – | – | – | – | – | – |
Lipid Particles | 0.0091 | 0.0009 | 0.0036 | 0.0092 | 0.0013 | 0.0052 | 0.0093 | 0.0018 | 0.0037 | 0.0037 | 0.0054 | 0.0039 | – | – | – | – | – | – |
Mitochondria | 0.0116 | 0.0025 | 0.0062 | 0.0022 | 0.0042 | 0.0052 | 0.0182 | 0.0048 | 0.0117 | 0.0024 | 0.0061 | 0.0106 | – | – | – | – | – | – |
None | 0.0127 | 0.0025 | 0.0121 | 0.0136 | 0.0004 | 0.0009 | 0.0152 | 0.0026 | 0.0088 | 0.0005 | 0.0401 | 0.0126 | – | – | – | – | – | – |
Nuclear Periphery | 0.0245 | 0.0155 | 0.012 | 0.011 | 0.0059 | 0.0141 | 0.0097 | 0.0127 | 0.0139 | 0.0038 | 0.004 | 0.0185 | – | – | – | – | – | – |
Nucleolus | 0.0031 | 0.0017 | 0.0019 | 0.0011 | 0.0006 | 0.0015 | 0.002 | 0.0018 | 0.0033 | 0.0016 | 0.0007 | 0.0013 | – | – | – | – | – | – |
Nucleus | 0.0105 | 0.0136 | 0.0056 | 0.0036 | 0.0019 | 0.0135 | 0.0107 | 0.0046 | 0.01 | 0.0018 | 0.0027 | 0.0043 | – | – | – | – | – | – |
Peroxisomes | 0.0068 | 0.0001 | 0.0005 | 0.0048 | 0.0002 | 0.0061 | 0.0088 | 0.0006 | 0.0019 | 0.0005 | 0.0003 | 0.0004 | – | – | – | – | – | – |
Punctate Nuclear | 0.0028 | 0.0002 | 0.0002 | 0.0052 | 0.0002 | 0.001 | 0.0009 | 0.0002 | 0.0007 | 0.0002 | 0.0004 | 0.0002 | – | – | – | – | – | – |
Vacuole | 0.4708 | 0.5879 | 0.5252 | 0.5727 | 0.506 | 0.4983 | 0.4399 | 0.5305 | 0.4265 | 0.4786 | 0.3914 | 0.4089 | – | – | – | – | – | – |
Vacuole Periphery | 0.1905 | 0.1747 | 0.1969 | 0.1775 | 0.2588 | 0.2442 | 0.2093 | 0.2429 | 0.207 | 0.1802 | 0.3081 | 0.2891 | – | – | – | – | – | – |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 29.3339 | 18.8265 | 9.9786 | 8.1089 | 8.7768 | 20.2968 | 19.8853 | 13.0771 | 15.6881 | 18.0346 |
Translational Efficiency | 0.5224 | 0.398 | 0.5743 | 0.8315 | 1.2283 | 0.9191 | 0.6416 | 0.8488 | 0.7656 | 0.7148 |
Dataset | Hit |
---|---|
Protein Concentration | – |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|