Standard name
Description Putative transporter, member of the sugar porter family; green fluorescent protein (GFP)-fusion protein localizes to the vacuolar membrane; YBR241C is not an essential gene; YBR241C has a paralog, VPS73, that arose from the whole genome duplication
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ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Cytoplasm 0.06 0.13 0.12 0.43 0.5 0.46 0.36 0.42 0.45 0.47 0.54 0.46 0.18 0.24 0.11 0.25 – – 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0.11 0.06 0.08 0.08 0.1 0.08 0.05 0.05 0.05 0 0 0 0.12 – – 0 0 0 0 0 0
Endosome 0.08 0 0.17 0.11 0.09 0 0 0 0 0 0 0 0 0.06 0.05 0 – – 0.08 0 0.12 0.05 0.08 0.1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Mitochondria 0 0 0 0.06 0 0.11 0.17 0.13 0.27 0.27 0.27 0.25 0 0 0 0 – – 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 – – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0.06 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Vac/Vac Membrane 0.91 0.87 0.82 0.39 0.49 0.41 0.45 0.39 0.29 0.3 0.26 0.33 0.81 0.79 0.91 0.67 – – 0.83 0.86 0.77 0.83 0.81 0.8
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 1 0 3 10 3 8 2 2 2 1 0 0 – – 0 0 0 0 1 0
Bud 0 0 0 0 0 0 5 1 4 2 3 4 0 1 0 0 – – 0 1 3 2 1 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – – –
Cell Periphery 0 0 0 0 1 1 4 11 16 14 10 11 0 0 0 0 – – 0 0 0 0 0 0
Cytoplasm 7 38 51 99 182 223 240 325 196 205 267 223 33 30 17 6 – – 0 6 1 1 1 3
Endoplasmic Reticulum 2 3 6 26 22 39 53 76 37 20 23 22 4 2 3 3 – – 0 1 3 0 2 1
Endosome 10 5 73 25 31 18 27 21 7 6 3 10 4 8 8 0 – – 11 13 54 7 21 23
Golgi 1 1 4 4 7 12 28 29 8 5 5 9 0 2 2 1 – – 0 2 2 0 1 0
Mitochondria 4 7 13 13 6 51 111 102 118 116 134 122 0 0 0 1 – – 3 3 4 1 2 3
Nucleus 2 10 5 1 2 5 5 7 3 2 5 0 9 5 4 0 – – 2 3 2 5 5 7
Nuclear Periphery 3 4 8 3 1 2 8 27 9 4 2 5 3 0 2 0 – – 3 1 26 5 6 4
Nucleolus 3 2 13 1 0 1 4 3 1 3 1 1 0 1 1 0 – – 0 1 1 1 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
SpindlePole 0 0 4 0 0 0 0 0 0 0 1 0 0 0 0 0 – – 0 0 1 0 0 1
Vac/Vac Membrane 108 250 354 91 177 200 298 306 127 131 131 163 149 100 144 16 – – 111 264 354 130 208 192
Unique Cell Count 119 289 434 231 362 484 669 781 437 434 499 487 184 127 159 24 135 307 460 156 257 242
Labelled Cell Count 140 320 531 264 430 552 786 918 529 516 587 572 204 150 181 27 135 307 460 156 257 242


Vacuole Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.9 8.7 7.7 7.8 9.3 8.9 9.2 10.0 9.4 9.4 9.5 10.1 9.1 10.1 11.3 10.0 – – 7.8 9.1 10.0
Std Deviation (1e-4) 1.7 1.8 1.7 1.8 2.3 2.3 2.3 2.6 2.8 2.9 3.0 3.3 2.3 2.5 3.3 3.0 – – 1.7 1.9 2.3
Intensity Change (Log2) – – – 0.01 0.26 0.21 0.25 0.37 0.28 0.28 0.29 0.38 0.23 0.38 0.54 0.36 – – 0.0 0.23 0.37


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Cell Periphery 0 0 0 0 0 4.0 3.8 0 3.1 0 0 0 0 – –
Cytoplasm 9.1 11.9 11.3 8.9 10.8 10.8 11.5 13.4 11.3 2.1 3.3 -0.4 0 – –
Endoplasmic Reticulum 5.7 3.6 4.7 4.7 5.6 4.8 2.8 2.8 2.8 0 0 0 0 – –
Endosome -2.1 -3.4 -6.6 -7.2 -8.8 -7.8 -7.9 -9.0 -7.8 -5.0 -3.0 -3.7 0 – –
Golgi 0 1.2 1.8 3.2 2.9 1.2 0 0 1.2 0 0 0 0 – –
Mitochondria 1.7 -1.2 4.5 7.0 5.7 9.9 9.8 10.0 9.4 0 0 0 0 – –
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Nuclear Periphery 0 0 0 -0.9 1.6 0.2 -1.2 0 -1.0 0 0 0 0 – –
Nucleolus 0 -3.3 -3.4 -3.2 -3.8 -3.2 -2.5 -3.5 -3.5 0 0 0 0 – –
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Vacuole -11.0 -9.7 -12.4 -12.2 -14.2 -15.6 -15.2 -16.9 -14.7 -0.2 -0.7 2.6 -1.8 – –