Standard name | |
---|---|
Human Ortholog | |
Description | Protein associated with U3 and U14 snoRNAs; required for pre-rRNA processing and 40S ribosomal subunit synthesis; localized in the nucleus and concentrated in the nucleolus; human BYSL functionally complements the heat sensitivity of an enp1 ts mutant |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 5 | 1 | 4 | 3 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 10 | 8 | 3 | 3 | 16 | 16 |
Bud Neck | 0 | 0 | 0 | 2 | 1 | 2 | 2 | 3 | 1 | 3 | 1 | 1 | 8 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 4 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 8 | 4 | 14 | 3 | 8 | 10 | 16 | 0 | 0 | 1 | 1 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 8 | 5 | 5 | 6 | 8 | 10 | 15 | 8 | 7 | 3 | 4 | 6 | 25 | 50 | 68 | 15 | 10 | 3 | 2 | 1 | 0 | 0 | 2 | 4 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Endosome | 11 | 2 | 6 | 12 | 6 | 4 | 17 | 25 | 2 | 11 | 8 | 9 | 18 | 5 | 13 | 10 | 7 | 0 | 17 | 3 | 10 | 14 | 38 | 47 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 0 | 1 | 0 | 0 | 0 | 0 |
Mitochondria | 13 | 2 | 4 | 15 | 15 | 50 | 72 | 107 | 56 | 91 | 82 | 113 | 9 | 21 | 28 | 30 | 10 | 10 | 1 | 0 | 1 | 0 | 1 | 1 |
Nucleus | 50 | 12 | 3 | 14 | 23 | 28 | 39 | 49 | 22 | 45 | 37 | 36 | 25 | 30 | 40 | 25 | 14 | 19 | 71 | 33 | 13 | 26 | 23 | 12 |
Nuclear Periphery | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 2 | 3 | 3 | 3 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 0 | 0 | 1 | 1 | 3 |
Nucleolus | 151 | 65 | 47 | 138 | 179 | 182 | 223 | 331 | 140 | 202 | 171 | 210 | 210 | 406 | 420 | 180 | 101 | 75 | 178 | 76 | 48 | 95 | 152 | 165 |
Peroxisomes | 0 | 0 | 1 | 0 | 0 | 3 | 1 | 5 | 1 | 1 | 1 | 0 | 1 | 3 | 7 | 3 | 3 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 0 | 1 | 13 | 16 | 24 | 41 | 68 | 11 | 29 | 32 | 23 | 6 | 32 | 43 | 1 | 2 | 0 | 34 | 12 | 12 | 43 | 93 | 79 |
Vac/Vac Membrane | 67 | 48 | 20 | 1 | 1 | 4 | 5 | 6 | 2 | 1 | 1 | 4 | 7 | 4 | 4 | 18 | 9 | 6 | 8 | 7 | 10 | 5 | 14 | 22 |
Unique Cell Count | 261 | 118 | 74 | 156 | 207 | 234 | 301 | 438 | 187 | 268 | 242 | 290 | 262 | 482 | 537 | 239 | 136 | 101 | 331 | 147 | 106 | 196 | 357 | 372 |
Labelled Cell Count | 301 | 134 | 87 | 202 | 250 | 316 | 423 | 621 | 249 | 401 | 353 | 426 | 309 | 551 | 626 | 286 | 161 | 115 | 331 | 147 | 106 | 196 | 357 | 372 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 12.9 | 14.9 | 11.6 | 13.6 | 11.3 | 9.2 | 7.5 | 7.5 | 7.2 | 6.6 | 6.9 | 6.7 | 15.3 | 15.5 | 14.3 | 19.7 | 22.3 | 24.7 | 15.3 | 14.1 | 15.5 |
Std Deviation (1e-4) | 2.6 | 3.1 | 2.8 | 4.2 | 3.2 | 2.5 | 2.4 | 2.0 | 2.1 | 1.8 | 2.0 | 2.0 | 4.4 | 6.3 | 6.8 | 6.4 | 7.6 | 7.6 | 2.6 | 3.2 | 2.9 |
Intensity Change (Log2) | – | – | – | 0.23 | -0.04 | -0.33 | -0.64 | -0.63 | -0.68 | -0.82 | -0.76 | -0.79 | 0.39 | 0.41 | 0.3 | 0.76 | 0.94 | 1.09 | 0.39 | 0.28 | 0.42 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.7 | 1.0 | 1.5 | 0 | 0.2 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | -0.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -0.4 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 1.1 | 0.5 | 3.1 | 3.5 | 3.7 | 4.2 | 4.9 | 4.8 | 5.5 | 0 | 0 | 0 | 1.7 | 0 | 1.1 |
Nucleus | 1.3 | 1.8 | 2.0 | 2.2 | 1.9 | 1.9 | 2.8 | 2.5 | 2.1 | 1.5 | 0 | 1.1 | 1.7 | 1.6 | 2.9 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 4.5 | 4.3 | 2.4 | 1.8 | 2.2 | 1.8 | 2.0 | 1.2 | 1.5 | 3.0 | 4.3 | 2.8 | 2.0 | 1.6 | 1.5 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 2.4 | 3.0 | 3.3 | 0 | 2.5 | 2.9 | 0 | 0 | 0 | 2.1 | 0 | 0 | 0 |
Vacuole | -6.5 | -7.5 | -7.1 | 0 | 0 | -6.8 | 0 | 0 | 0 | -6.8 | 0 | 0 | -4.5 | -4.1 | -3.9 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 9.0149 | 11.5901 | 9.8701 | 10.0181 | 8.7015 | 9.9735 | 9.1217 | 12.112 | 10.7979 | 8.8997 | 9.9743 | 10.85 | 10.9788 | 14.4926 | 12.815 | 12.2004 | 11.919 | 12.562 |
Actin | 0.0089 | 0.0003 | 0 | 0.0001 | 0.0011 | 0.0005 | 0.0195 | 0 | 0.0037 | 0.0008 | 0.0046 | 0.0027 | 0.0286 | 0 | 0.0033 | 0.028 | 0.0052 | 0.0008 |
Bud | 0.0007 | 0.0001 | 0.0002 | 0.0003 | 0.0003 | 0.0002 | 0.0006 | 0.0002 | 0.0002 | 0.001 | 0.0002 | 0.0004 | 0.0003 | 0.0002 | 0.0002 | 0.0003 | 0.0006 | 0.0001 |
Bud Neck | 0.0039 | 0.0016 | 0.002 | 0.0021 | 0.0026 | 0.0042 | 0.0035 | 0.0027 | 0.0106 | 0.0066 | 0.0051 | 0.0064 | 0.0029 | 0.0038 | 0.0025 | 0.0066 | 0.0028 | 0.0083 |
Bud Periphery | 0.0013 | 0.0001 | 0.0003 | 0.0003 | 0.0006 | 0.0007 | 0.0011 | 0.0001 | 0.0003 | 0.0015 | 0.0004 | 0.0007 | 0.0005 | 0.0002 | 0.0003 | 0.0005 | 0.0008 | 0.0003 |
Bud Site | 0.0037 | 0.0011 | 0.0031 | 0.0011 | 0.0013 | 0.0009 | 0.0042 | 0.0031 | 0.003 | 0.0079 | 0.002 | 0.0019 | 0.0031 | 0.0056 | 0.0021 | 0.0023 | 0.0047 | 0.001 |
Cell Periphery | 0.0005 | 0.0002 | 0.0005 | 0.0004 | 0.0006 | 0.0004 | 0.0011 | 0.0003 | 0.0009 | 0.0012 | 0.0004 | 0.0004 | 0.0004 | 0.0006 | 0.0006 | 0.0011 | 0.0004 | 0.0005 |
Cytoplasm | 0.0068 | 0.0007 | 0.0003 | 0.0074 | 0.0007 | 0.0002 | 0.0216 | 0.0019 | 0.0007 | 0.0037 | 0.0322 | 0.0023 | 0.0061 | 0.0169 | 0.0012 | 0.0005 | 0.0082 | 0.0003 |
Cytoplasmic Foci | 0.0095 | 0.0027 | 0.001 | 0.0018 | 0.0032 | 0.0019 | 0.0223 | 0.0022 | 0.0225 | 0.0117 | 0.024 | 0.0104 | 0.0183 | 0.0045 | 0.0058 | 0.003 | 0.03 | 0.0036 |
Eisosomes | 0.0001 | 0 | 0 | 0 | 0.0002 | 0 | 0.0002 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0.0003 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0037 | 0.0001 | 0.0001 | 0.0008 | 0.0003 | 0.0003 | 0.0037 | 0.0004 | 0.0002 | 0.0012 | 0.0035 | 0.0006 | 0.0029 | 0.0001 | 0.0016 | 0.0001 | 0.0018 | 0.0001 |
Endosome | 0.015 | 0.001 | 0.0007 | 0.0086 | 0.0051 | 0.0043 | 0.0332 | 0.0033 | 0.0115 | 0.01 | 0.0126 | 0.0059 | 0.0139 | 0.0026 | 0.0115 | 0.0026 | 0.0243 | 0.002 |
Golgi | 0.0037 | 0.0004 | 0.0002 | 0.0004 | 0.0013 | 0.0065 | 0.0149 | 0.0002 | 0.0037 | 0.0022 | 0.0034 | 0.0023 | 0.0067 | 0.0005 | 0.0075 | 0.0021 | 0.0202 | 0.0023 |
Lipid Particles | 0.0101 | 0.004 | 0.0031 | 0.0033 | 0.0094 | 0.0064 | 0.0258 | 0.0021 | 0.0159 | 0.0461 | 0.0123 | 0.0058 | 0.0241 | 0.0042 | 0.0122 | 0.0115 | 0.032 | 0.0078 |
Mitochondria | 0.0042 | 0.0019 | 0.0002 | 0.0006 | 0.1027 | 0.0133 | 0.0062 | 0.0001 | 0.0004 | 0.005 | 0.0678 | 0.0081 | 0.0035 | 0.0001 | 0.0031 | 0.0005 | 0.0271 | 0.0005 |
None | 0.0023 | 0.0004 | 0 | 0.0003 | 0.0003 | 0.0001 | 0.0036 | 0.0001 | 0.0004 | 0.0016 | 0.0059 | 0.0004 | 0.0103 | 0.0001 | 0.0003 | 0.0001 | 0.0012 | 0 |
Nuclear Periphery | 0.0105 | 0.0009 | 0.0007 | 0.0089 | 0.0043 | 0.0058 | 0.0316 | 0.0037 | 0.0003 | 0.0096 | 0.0214 | 0.0079 | 0.014 | 0.0005 | 0.005 | 0.0016 | 0.0039 | 0.0029 |
Nucleolus | 0.6573 | 0.7943 | 0.8273 | 0.6179 | 0.6315 | 0.7345 | 0.5053 | 0.7073 | 0.8172 | 0.703 | 0.5764 | 0.6938 | 0.6092 | 0.7387 | 0.7878 | 0.6773 | 0.5556 | 0.7046 |
Nucleus | 0.2144 | 0.1705 | 0.1463 | 0.3243 | 0.182 | 0.1945 | 0.2242 | 0.2475 | 0.0923 | 0.1256 | 0.163 | 0.2168 | 0.2055 | 0.21 | 0.1439 | 0.2412 | 0.2472 | 0.2351 |
Peroxisomes | 0.005 | 0.0011 | 0.0001 | 0.0002 | 0.0012 | 0.0075 | 0.013 | 0.0001 | 0.0038 | 0.0012 | 0.0035 | 0.0036 | 0.0059 | 0.0002 | 0.0012 | 0.0012 | 0.0113 | 0.0006 |
Punctate Nuclear | 0.0327 | 0.0176 | 0.0087 | 0.0106 | 0.0395 | 0.0128 | 0.0465 | 0.0213 | 0.0067 | 0.0338 | 0.0587 | 0.0268 | 0.0369 | 0.0068 | 0.0052 | 0.0121 | 0.0161 | 0.0265 |
Vacuole | 0.0041 | 0.0008 | 0.0046 | 0.0088 | 0.0094 | 0.0033 | 0.015 | 0.0031 | 0.0052 | 0.0211 | 0.0018 | 0.0024 | 0.0043 | 0.0041 | 0.0039 | 0.007 | 0.0034 | 0.0023 |
Vacuole Periphery | 0.0015 | 0.0002 | 0.0005 | 0.0017 | 0.0025 | 0.0018 | 0.0031 | 0.0002 | 0.0003 | 0.0053 | 0.0006 | 0.0004 | 0.0023 | 0.0002 | 0.0006 | 0.0004 | 0.003 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 62.3135 | 60.4597 | 53.9373 | 39.0858 | 45.3224 | 43.2915 | 41.3834 | 37.5504 | 28.5546 | 46.9461 |
Translational Efficiency | 1.8041 | 1.8205 | 1.7687 | 1.7739 | 1.7786 | 1.5339 | 1.8361 | 1.2346 | 1.5073 | 1.4443 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1215 | 1373 | 1580 | 875 | 1809 | 1572 | 127 | 1349 | 3024 | 2945 | 1707 | 2224 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1016.62 | 1205.38 | 1476.09 | 1893.38 | 1226.59 | 1297.59 | 1871.65 | 1952.80 | 1142.23 | 1254.60 | 1505.52 | 1929.42 |
Standard Deviation | 186.02 | 264.18 | 246.88 | 404.24 | 204.09 | 245.36 | 314.47 | 444.86 | 222.30 | 258.43 | 273.04 | 430.32 |
Intensity Change Log 2 | — | 0.245708 | 0.538000 | 0.897183 | — | 0.081182 | 0.609658 | 0.670891 | — | 0.158076 | 0.577623 | 0.777862 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000598 | 0.000946 | 0.000579 | 0.000725 | 0.000506 | 0.000798 | 0.000711 | 0.000766 | 0.000543 | 0.000867 | 0.000589 | 0.000750 |
Bud Neck | 0.013239 | 0.039638 | 0.026041 | 0.034762 | 0.020721 | 0.038225 | 0.019852 | 0.036453 | 0.017714 | 0.038884 | 0.025581 | 0.035787 |
Bud Site | 0.003483 | 0.006795 | 0.002817 | 0.004709 | 0.002181 | 0.007356 | 0.003291 | 0.006962 | 0.002704 | 0.007094 | 0.002852 | 0.006075 |
Cell Periphery | 0.000230 | 0.000287 | 0.000081 | 0.000224 | 0.000114 | 0.000171 | 0.000081 | 0.000310 | 0.000160 | 0.000225 | 0.000081 | 0.000276 |
Cytoplasm | 0.017355 | 0.027368 | 0.005125 | 0.001517 | 0.005957 | 0.010455 | 0.000451 | 0.004194 | 0.010537 | 0.018340 | 0.004777 | 0.003141 |
Cytoplasmic Foci | 0.002257 | 0.004362 | 0.000571 | 0.004582 | 0.001096 | 0.003182 | 0.006488 | 0.005510 | 0.001562 | 0.003732 | 0.001011 | 0.005145 |
Eisosomes | 0.000044 | 0.000045 | 0.000032 | 0.000078 | 0.000045 | 0.000050 | 0.000099 | 0.000087 | 0.000045 | 0.000048 | 0.000037 | 0.000083 |
Endoplasmic Reticulum | 0.002907 | 0.002881 | 0.000788 | 0.000601 | 0.002783 | 0.002428 | 0.000579 | 0.000801 | 0.002833 | 0.002639 | 0.000773 | 0.000722 |
Endosome | 0.001721 | 0.003412 | 0.000869 | 0.002078 | 0.002410 | 0.004829 | 0.000429 | 0.003073 | 0.002133 | 0.004168 | 0.000836 | 0.002681 |
Golgi | 0.001460 | 0.002027 | 0.000756 | 0.001000 | 0.000657 | 0.002887 | 0.000791 | 0.002255 | 0.000979 | 0.002486 | 0.000758 | 0.001761 |
Lipid Particles | 0.000388 | 0.000507 | 0.000113 | 0.000258 | 0.000370 | 0.001268 | 0.000235 | 0.000286 | 0.000377 | 0.000913 | 0.000122 | 0.000275 |
Mitochondria | 0.001306 | 0.002647 | 0.002173 | 0.003893 | 0.001413 | 0.002903 | 0.002100 | 0.005102 | 0.001370 | 0.002784 | 0.002167 | 0.004627 |
Mitotic Spindle | 0.005106 | 0.013286 | 0.021080 | 0.018140 | 0.006645 | 0.031191 | 0.016095 | 0.020392 | 0.006027 | 0.022843 | 0.020709 | 0.019506 |
None | 0.007836 | 0.007715 | 0.001854 | 0.001226 | 0.002570 | 0.003328 | 0.000685 | 0.001669 | 0.004685 | 0.005373 | 0.001767 | 0.001495 |
Nuclear Periphery | 0.001181 | 0.003736 | 0.002080 | 0.005125 | 0.004660 | 0.003988 | 0.001365 | 0.004939 | 0.003262 | 0.003871 | 0.002027 | 0.005012 |
Nuclear Periphery Foci | 0.012059 | 0.020295 | 0.002063 | 0.003371 | 0.025988 | 0.014981 | 0.002410 | 0.002614 | 0.020391 | 0.017458 | 0.002089 | 0.002912 |
Nucleolus | 0.428101* | 0.367354* | 0.518698* | 0.598495* | 0.485208* | 0.445630* | 0.715196* | 0.561670* | 0.462263* | 0.409137* | 0.533317* | 0.576159* |
Nucleus | 0.495193* | 0.484269* | 0.407267* | 0.295290* | 0.431208* | 0.414492* | 0.198450* | 0.315424* | 0.456916* | 0.447023* | 0.391731* | 0.307502* |
Peroxisomes | 0.001837 | 0.002536 | 0.003354 | 0.010541 | 0.001534 | 0.002683 | 0.024393 | 0.008997 | 0.001656 | 0.002615 | 0.004919 | 0.009605 |
Vacuole | 0.002556 | 0.007274 | 0.002554 | 0.007380 | 0.002544 | 0.006334 | 0.004668 | 0.009728 | 0.002549 | 0.006772 | 0.002711 | 0.008804 |
Vacuole Periphery | 0.001146 | 0.002621 | 0.001106 | 0.006005 | 0.001392 | 0.002821 | 0.001629 | 0.008769 | 0.001293 | 0.002728 | 0.001145 | 0.007681 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -7.00 | -4.86 | -2.74 | 4.01 | 1.22 | -5.81 | -3.48 | -6.10 | 1.25 | 1.31 | -9.08 | -7.29 | -6.43 | 3.62 | 1.12 |
Bud Neck | -15.22 | -15.94 | -12.39 | 0.13 | -1.50 | -11.12 | -1.81 | -12.28 | -2.01 | -3.77 | -18.03 | -13.94 | -17.31 | -1.45 | -3.53 |
Bud Site | -6.90 | -3.07 | -3.38 | 2.79 | -1.19 | -6.47 | -3.48 | -7.82 | -0.02 | -2.08 | -9.42 | -6.25 | -8.38 | 1.13 | -3.73 |
Cell Periphery | -1.55 | 3.22 | -0.24 | 1.53 | -4.51 | -3.31 | -0.55 | -5.26 | -3.56 | -4.41 | -3.37 | 3.28 | -4.17 | -1.98 | -7.02 |
Cytoplasm | -3.07 | 5.07 | 6.36 | 10.24 | 2.80 | -3.14 | 5.29 | -0.03 | 3.08 | -5.16 | -4.77 | 4.59 | 4.47 | 9.40 | -0.31 |
Cytoplasmic Foci | -3.38 | 3.62 | -2.67 | 0.08 | -4.61 | -3.92 | -3.83 | -5.44 | -3.33 | 0.49 | -5.24 | 1.49 | -6.02 | -2.56 | -6.61 |
Eisosomes | -0.36 | 6.29 | -7.59 | -7.56 | -11.12 | -2.36 | -5.78 | -6.90 | -5.82 | 2.19 | -2.04 | 3.75 | -9.59 | -8.72 | -11.42 |
Endoplasmic Reticulum | -0.16 | 9.93 | 11.50 | 11.27 | 4.60 | 1.30 | 9.39 | 8.19 | 9.01 | 0.23 | 0.79 | 12.72 | 12.47 | 13.53 | 3.03 |
Endosome | -5.06 | 2.89 | -0.19 | 2.44 | -1.48 | -4.13 | 7.79 | 0.20 | 2.98 | -2.97 | -5.99 | 5.46 | 0.04 | 3.74 | -2.78 |
Golgi | -1.16 | 1.60 | 1.17 | 2.71 | -0.89 | -3.34 | -2.37 | -2.79 | 1.06 | -2.10 | -3.58 | 0.71 | -1.79 | 1.89 | -2.36 |
Lipid Particles | -2.07 | 2.26 | 0.61 | 2.89 | -1.53 | -2.42 | 1.53 | 1.56 | 2.74 | -0.05 | -2.86 | 4.08 | 1.51 | 3.36 | -1.93 |
Mitochondria | -4.96 | -7.14 | -10.37 | -4.57 | -6.10 | -6.50 | -3.61 | -9.52 | -4.87 | -4.84 | -8.17 | -10.29 | -13.01 | -6.46 | -7.84 |
Mitotic Spindle | -4.40 | -8.95 | -4.54 | -1.45 | 2.32 | -8.03 | -1.44 | -5.97 | 1.74 | -0.47 | -8.99 | -9.05 | -7.50 | 0.23 | 2.23 |
None | 0.18 | 5.44 | 6.39 | 10.86 | 5.19 | -1.55 | 3.59 | 1.30 | 4.80 | -6.55 | -1.22 | 4.78 | 5.86 | 11.48 | 3.02 |
Nuclear Periphery | -6.32 | -3.87 | -5.20 | -1.49 | -3.74 | 0.57 | 4.75 | 0.10 | -0.51 | -5.06 | -1.73 | 2.65 | -2.85 | -1.39 | -5.64 |
Nuclear Periphery Foci | -3.60 | 6.51 | 5.43 | 8.92 | -1.76 | 4.09 | 10.87 | 11.00 | 8.00 | -0.53 | 1.46 | 12.75 | 12.23 | 12.08 | -1.61 |
Nucleolus | 6.67 | -4.68 | -10.45 | -16.48 | -6.60 | 5.15 | -6.93 | -2.65 | -6.76 | 5.59 | 8.88 | -3.73 | -8.65 | -15.91 | -3.86 |
Nucleus | 1.76 | 7.94 | 15.74 | 14.63 | 8.96 | 2.32 | 9.09 | 9.08 | 6.63 | -4.84 | 2.25 | 6.89 | 16.99 | 14.72 | 7.64 |
Peroxisomes | -2.87 | -7.12 | -10.68 | -9.65 | -8.24 | -5.69 | -9.92 | -13.02 | -9.98 | 6.80 | -6.29 | -12.23 | -16.80 | -13.95 | -7.42 |
Vacuole | -6.98 | -1.51 | -4.37 | 0.59 | -3.21 | -5.34 | -2.30 | -5.82 | -1.71 | -3.13 | -8.50 | -1.38 | -7.26 | -1.07 | -5.45 |
Vacuole Periphery | -6.36 | 0.06 | -8.36 | -5.31 | -8.55 | -4.26 | -1.45 | -8.99 | -6.38 | -8.53 | -6.99 | 1.39 | -11.84 | -8.18 | -12.17 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|