Standard name
Description Protein associated with U3 and U14 snoRNAs; required for pre-rRNA processing and 40S ribosomal subunit synthesis; localized in the nucleus and concentrated in the nucleolus; human BYSL functionally complements the heat sensitivity of an enp1 ts mutant
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0.05 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.07 0 0 0 0.05 0 0 0 0 0 0.1 0.1 0.13 0.06 0.07 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0.08 0.08 0 0 0.06 0.06 0 0 0 0 0.07 0 0 0 0.05 0 0.05 0 0.1 0.07 0.11 0.13
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0 0.05 0.1 0.07 0.21 0.24 0.24 0.3 0.34 0.34 0.39 0 0 0.05 0.13 0.07 0.1 0 0 0 0 0 0
Nucleus 0.19 0.1 0 0.09 0.11 0.12 0.13 0.11 0.12 0.17 0.15 0.12 0.1 0.06 0.07 0.1 0.1 0.19 0.22 0.22 0.12 0.13 0.07 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.58 0.55 0.64 0.88 0.86 0.78 0.74 0.76 0.75 0.75 0.71 0.72 0.8 0.84 0.78 0.75 0.74 0.74 0.54 0.52 0.45 0.49 0.43 0.45
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0.08 0.08 0.1 0.14 0.16 0.06 0.11 0.13 0.08 0 0.07 0.08 0 0 0 0.1 0.08 0.11 0.22 0.26 0.21
Vac/Vac Membrane 0.26 0.41 0.27 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.07 0.06 0 0.05 0.1 0 0 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 3 5 1 4 3 5 0 0 0 0 0 0 10 8 3 3 16 16
Bud Neck 0 0 0 2 1 2 2 3 1 3 1 1 8 0 1 1 0 0 0 1 0 0 2 4
Bud Site 0 0 0 0 0 8 4 14 3 8 10 16 0 0 1 1 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Cytoplasm 8 5 5 6 8 10 15 8 7 3 4 6 25 50 68 15 10 3 2 1 0 0 2 4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 3 0 0 0 0 0 1 0
Endosome 11 2 6 12 6 4 17 25 2 11 8 9 18 5 13 10 7 0 17 3 10 14 38 47
Golgi 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 2 2 0 1 0 0 0 0
Mitochondria 13 2 4 15 15 50 72 107 56 91 82 113 9 21 28 30 10 10 1 0 1 0 1 1
Nucleus 50 12 3 14 23 28 39 49 22 45 37 36 25 30 40 25 14 19 71 33 13 26 23 12
Nuclear Periphery 0 0 0 1 1 1 0 0 2 3 3 3 0 0 1 0 0 0 2 0 0 1 1 3
Nucleolus 151 65 47 138 179 182 223 331 140 202 171 210 210 406 420 180 101 75 178 76 48 95 152 165
Peroxisomes 0 0 1 0 0 3 1 5 1 1 1 0 1 3 7 3 3 0 1 0 0 0 0 0
SpindlePole 1 0 1 13 16 24 41 68 11 29 32 23 6 32 43 1 2 0 34 12 12 43 93 79
Vac/Vac Membrane 67 48 20 1 1 4 5 6 2 1 1 4 7 4 4 18 9 6 8 7 10 5 14 22
Unique Cell Count 261 118 74 156 207 234 301 438 187 268 242 290 262 482 537 239 136 101 331 147 106 196 357 372
Labelled Cell Count 301 134 87 202 250 316 423 621 249 401 353 426 309 551 626 286 161 115 331 147 106 196 357 372


Cytoplasm, Nucleolus, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 12.9 14.9 11.6 13.6 11.3 9.2 7.5 7.5 7.2 6.6 6.9 6.7 15.3 15.5 14.3 19.7 22.3 24.7 15.3 14.1 15.5
Std Deviation (1e-4) 2.6 3.1 2.8 4.2 3.2 2.5 2.4 2.0 2.1 1.8 2.0 2.0 4.4 6.3 6.8 6.4 7.6 7.6 2.6 3.2 2.9
Intensity Change (Log2) – – – 0.23 -0.04 -0.33 -0.64 -0.63 -0.68 -0.82 -0.76 -0.79 0.39 0.41 0.3 0.76 0.94 1.09 0.39 0.28 0.42


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0.7 1.0 1.5 0 0.2 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome -0.1 0 0 0 0 0 0 0 0 -0.4 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 1.1 0.5 3.1 3.5 3.7 4.2 4.9 4.8 5.5 0 0 0 1.7 0 1.1
Nucleus 1.3 1.8 2.0 2.2 1.9 1.9 2.8 2.5 2.1 1.5 0 1.1 1.7 1.6 2.9
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 4.5 4.3 2.4 1.8 2.2 1.8 2.0 1.2 1.5 3.0 4.3 2.8 2.0 1.6 1.5
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 2.4 3.0 3.3 0 2.5 2.9 0 0 0 2.1 0 0 0
Vacuole -6.5 -7.5 -7.1 0 0 -6.8 0 0 0 -6.8 0 0 -4.5 -4.1 -3.9