Standard name
Human Ortholog
Description Protein associated with U3 and U14 snoRNAs; required for pre-rRNA processing and 40S ribosomal subunit synthesis; localized in the nucleus and concentrated in the nucleolus; human BYSL functionally complements the heat sensitivity of an enp1 ts mutant
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0.05 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.07 0 0 0 0.05 0 0 0 0 0 0.1 0.1 0.13 0.06 0.07 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0.08 0.08 0 0 0.06 0.06 0 0 0 0 0.07 0 0 0 0.05 0 0.05 0 0.1 0.07 0.11 0.13
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0 0.05 0.1 0.07 0.21 0.24 0.24 0.3 0.34 0.34 0.39 0 0 0.05 0.13 0.07 0.1 0 0 0 0 0 0
Nucleus 0.19 0.1 0 0.09 0.11 0.12 0.13 0.11 0.12 0.17 0.15 0.12 0.1 0.06 0.07 0.1 0.1 0.19 0.22 0.22 0.12 0.13 0.07 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.58 0.55 0.64 0.88 0.86 0.78 0.74 0.76 0.75 0.75 0.71 0.72 0.8 0.84 0.78 0.75 0.74 0.74 0.54 0.52 0.45 0.49 0.43 0.45
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0.08 0.08 0.1 0.14 0.16 0.06 0.11 0.13 0.08 0 0.07 0.08 0 0 0 0.1 0.08 0.11 0.22 0.26 0.21
Vac/Vac Membrane 0.26 0.41 0.27 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.07 0.06 0 0.05 0.1 0 0 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 3 5 1 4 3 5 0 0 0 0 0 0 10 8 3 3 16 16
Bud Neck 0 0 0 2 1 2 2 3 1 3 1 1 8 0 1 1 0 0 0 1 0 0 2 4
Bud Site 0 0 0 0 0 8 4 14 3 8 10 16 0 0 1 1 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Cytoplasm 8 5 5 6 8 10 15 8 7 3 4 6 25 50 68 15 10 3 2 1 0 0 2 4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 3 0 0 0 0 0 1 0
Endosome 11 2 6 12 6 4 17 25 2 11 8 9 18 5 13 10 7 0 17 3 10 14 38 47
Golgi 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 2 2 0 1 0 0 0 0
Mitochondria 13 2 4 15 15 50 72 107 56 91 82 113 9 21 28 30 10 10 1 0 1 0 1 1
Nucleus 50 12 3 14 23 28 39 49 22 45 37 36 25 30 40 25 14 19 71 33 13 26 23 12
Nuclear Periphery 0 0 0 1 1 1 0 0 2 3 3 3 0 0 1 0 0 0 2 0 0 1 1 3
Nucleolus 151 65 47 138 179 182 223 331 140 202 171 210 210 406 420 180 101 75 178 76 48 95 152 165
Peroxisomes 0 0 1 0 0 3 1 5 1 1 1 0 1 3 7 3 3 0 1 0 0 0 0 0
SpindlePole 1 0 1 13 16 24 41 68 11 29 32 23 6 32 43 1 2 0 34 12 12 43 93 79
Vac/Vac Membrane 67 48 20 1 1 4 5 6 2 1 1 4 7 4 4 18 9 6 8 7 10 5 14 22
Unique Cell Count 261 118 74 156 207 234 301 438 187 268 242 290 262 482 537 239 136 101 331 147 106 196 357 372
Labelled Cell Count 301 134 87 202 250 316 423 621 249 401 353 426 309 551 626 286 161 115 331 147 106 196 357 372


Cytoplasm, Nucleolus, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 12.9 14.9 11.6 13.6 11.3 9.2 7.5 7.5 7.2 6.6 6.9 6.7 15.3 15.5 14.3 19.7 22.3 24.7 15.3 14.1 15.5
Std Deviation (1e-4) 2.6 3.1 2.8 4.2 3.2 2.5 2.4 2.0 2.1 1.8 2.0 2.0 4.4 6.3 6.8 6.4 7.6 7.6 2.6 3.2 2.9
Intensity Change (Log2) – – – 0.23 -0.04 -0.33 -0.64 -0.63 -0.68 -0.82 -0.76 -0.79 0.39 0.41 0.3 0.76 0.94 1.09 0.39 0.28 0.42


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0.7 1.0 1.5 0 0.2 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome -0.1 0 0 0 0 0 0 0 0 -0.4 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 1.1 0.5 3.1 3.5 3.7 4.2 4.9 4.8 5.5 0 0 0 1.7 0 1.1
Nucleus 1.3 1.8 2.0 2.2 1.9 1.9 2.8 2.5 2.1 1.5 0 1.1 1.7 1.6 2.9
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 4.5 4.3 2.4 1.8 2.2 1.8 2.0 1.2 1.5 3.0 4.3 2.8 2.0 1.6 1.5
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 2.4 3.0 3.3 0 2.5 2.9 0 0 0 2.1 0 0 0
Vacuole -6.5 -7.5 -7.1 0 0 -6.8 0 0 0 -6.8 0 0 -4.5 -4.1 -3.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 9.0149 11.5901 9.8701 10.0181 8.7015 9.9735 9.1217 12.112 10.7979 8.8997 9.9743 10.85 10.9788 14.4926 12.815 12.2004 11.919 12.562
Actin 0.0089 0.0003 0 0.0001 0.0011 0.0005 0.0195 0 0.0037 0.0008 0.0046 0.0027 0.0286 0 0.0033 0.028 0.0052 0.0008
Bud 0.0007 0.0001 0.0002 0.0003 0.0003 0.0002 0.0006 0.0002 0.0002 0.001 0.0002 0.0004 0.0003 0.0002 0.0002 0.0003 0.0006 0.0001
Bud Neck 0.0039 0.0016 0.002 0.0021 0.0026 0.0042 0.0035 0.0027 0.0106 0.0066 0.0051 0.0064 0.0029 0.0038 0.0025 0.0066 0.0028 0.0083
Bud Periphery 0.0013 0.0001 0.0003 0.0003 0.0006 0.0007 0.0011 0.0001 0.0003 0.0015 0.0004 0.0007 0.0005 0.0002 0.0003 0.0005 0.0008 0.0003
Bud Site 0.0037 0.0011 0.0031 0.0011 0.0013 0.0009 0.0042 0.0031 0.003 0.0079 0.002 0.0019 0.0031 0.0056 0.0021 0.0023 0.0047 0.001
Cell Periphery 0.0005 0.0002 0.0005 0.0004 0.0006 0.0004 0.0011 0.0003 0.0009 0.0012 0.0004 0.0004 0.0004 0.0006 0.0006 0.0011 0.0004 0.0005
Cytoplasm 0.0068 0.0007 0.0003 0.0074 0.0007 0.0002 0.0216 0.0019 0.0007 0.0037 0.0322 0.0023 0.0061 0.0169 0.0012 0.0005 0.0082 0.0003
Cytoplasmic Foci 0.0095 0.0027 0.001 0.0018 0.0032 0.0019 0.0223 0.0022 0.0225 0.0117 0.024 0.0104 0.0183 0.0045 0.0058 0.003 0.03 0.0036
Eisosomes 0.0001 0 0 0 0.0002 0 0.0002 0 0 0.0001 0.0001 0 0.0003 0 0.0001 0.0001 0.0001 0
Endoplasmic Reticulum 0.0037 0.0001 0.0001 0.0008 0.0003 0.0003 0.0037 0.0004 0.0002 0.0012 0.0035 0.0006 0.0029 0.0001 0.0016 0.0001 0.0018 0.0001
Endosome 0.015 0.001 0.0007 0.0086 0.0051 0.0043 0.0332 0.0033 0.0115 0.01 0.0126 0.0059 0.0139 0.0026 0.0115 0.0026 0.0243 0.002
Golgi 0.0037 0.0004 0.0002 0.0004 0.0013 0.0065 0.0149 0.0002 0.0037 0.0022 0.0034 0.0023 0.0067 0.0005 0.0075 0.0021 0.0202 0.0023
Lipid Particles 0.0101 0.004 0.0031 0.0033 0.0094 0.0064 0.0258 0.0021 0.0159 0.0461 0.0123 0.0058 0.0241 0.0042 0.0122 0.0115 0.032 0.0078
Mitochondria 0.0042 0.0019 0.0002 0.0006 0.1027 0.0133 0.0062 0.0001 0.0004 0.005 0.0678 0.0081 0.0035 0.0001 0.0031 0.0005 0.0271 0.0005
None 0.0023 0.0004 0 0.0003 0.0003 0.0001 0.0036 0.0001 0.0004 0.0016 0.0059 0.0004 0.0103 0.0001 0.0003 0.0001 0.0012 0
Nuclear Periphery 0.0105 0.0009 0.0007 0.0089 0.0043 0.0058 0.0316 0.0037 0.0003 0.0096 0.0214 0.0079 0.014 0.0005 0.005 0.0016 0.0039 0.0029
Nucleolus 0.6573 0.7943 0.8273 0.6179 0.6315 0.7345 0.5053 0.7073 0.8172 0.703 0.5764 0.6938 0.6092 0.7387 0.7878 0.6773 0.5556 0.7046
Nucleus 0.2144 0.1705 0.1463 0.3243 0.182 0.1945 0.2242 0.2475 0.0923 0.1256 0.163 0.2168 0.2055 0.21 0.1439 0.2412 0.2472 0.2351
Peroxisomes 0.005 0.0011 0.0001 0.0002 0.0012 0.0075 0.013 0.0001 0.0038 0.0012 0.0035 0.0036 0.0059 0.0002 0.0012 0.0012 0.0113 0.0006
Punctate Nuclear 0.0327 0.0176 0.0087 0.0106 0.0395 0.0128 0.0465 0.0213 0.0067 0.0338 0.0587 0.0268 0.0369 0.0068 0.0052 0.0121 0.0161 0.0265
Vacuole 0.0041 0.0008 0.0046 0.0088 0.0094 0.0033 0.015 0.0031 0.0052 0.0211 0.0018 0.0024 0.0043 0.0041 0.0039 0.007 0.0034 0.0023
Vacuole Periphery 0.0015 0.0002 0.0005 0.0017 0.0025 0.0018 0.0031 0.0002 0.0003 0.0053 0.0006 0.0004 0.0023 0.0002 0.0006 0.0004 0.003 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 62.3135 60.4597 53.9373 39.0858 45.3224 43.2915 41.3834 37.5504 28.5546 46.9461
Translational Efficiency 1.8041 1.8205 1.7687 1.7739 1.7786 1.5339 1.8361 1.2346 1.5073 1.4443

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Protein associated with U3 and U14 snoRNAs; required for pre-rRNA processing and 40S ribosomal subunit synthesis; localized in the nucleus and concentrated in the nucleolus; human BYSL functionally complements the heat sensitivity of an enp1 ts mutant
Localization
Cell Percentages nucleolus (26%), nucleus (21%), mixed (46%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Enp1

Enp1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Enp1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available