Standard name
Human Ortholog
Description Core component of transport protein particle (TRAPP) complexes I-III; TRAPPs are multimeric guanine nucleotide-exchange factors for GTPase Ypt1p, regulating ER-Golgi traffic (TRAPPI), intra-Golgi traffic (TRAPPII), endosome-Golgi traffic (TRAPPII and III) and autophagy (TRAPPIII); mutation leads to defects in endocytic recycling, block in sporulation/meiosis; mutations in human homolog TRAPPC2 cause spondyloepiphyseal dysplasia tarda, TRAPPC2 can complement yeast null mutant

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.19 0.26 0 0 0 0 0 0 0 0 0 0.34 0.16 0 0.34 0.2 0.18 0.08 0.14 0.07 0.07 0.11 0.12
Bud 0 0 0.09 0.06 0.06 0.09 0.07 0.07 0.09 0.12 0.11 0.05 0 0 0.05 0.05 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Cytoplasm 0.08 0.23 0.19 0.19 0.12 0.14 0.11 0.15 0.09 0.11 0.11 0.35 0.22 0.16 0.19 0.3 0.22 0 0 0 0 0 0
Endoplasmic Reticulum 0.06 0.15 0 0 0 0 0 0 0 0 0 0.14 0.12 0 0.06 0.07 0.11 0 0 0 0 0 0
Endosome 0.41 0.24 0.18 0.35 0.25 0 0.06 0.06 0.06 0 0 0.2 0.42 0.58 0.13 0.28 0.31 0.42 0.35 0.38 0.37 0.28 0.27
Golgi 0.2 0.17 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0.39 0.17 0.21 0.28 0.2 0.21 0.2 0.26 0.26
Mitochondria 0.17 0.15 0.58 0.4 0.59 0.8 0.8 0.71 0.76 0.84 0.78 0 0 0 0.14 0.15 0.07 0.05 0.09 0.11 0.18 0.15 0.14
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0.06 0.15 0.13 0 0.06 0.05 0.09 0.05 0.07 0 0 0.07 0 0 0 0.09 0.05 0.11 0.06 0 0
SpindlePole 0.12 0 0.12 0.13 0.1 0.08 0.08 0.14 0.14 0.12 0.12 0.07 0.11 0.15 0.08 0 0.12 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0.12 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 31 37 4 1 4 4 2 0 1 0 5 96 45 13 26 8 13 19 31 8 16 38 56
Bud 5 2 9 11 20 31 32 33 48 48 55 14 4 8 4 2 0 1 5 0 2 6 2
Bud Neck 0 0 0 0 0 1 0 2 2 0 0 1 1 0 0 0 0 1 3 0 1 4 3
Bud Site 0 0 1 1 0 6 5 2 7 6 12 0 0 0 0 0 0
Cell Periphery 1 4 0 1 0 0 1 0 1 1 2 2 6 0 4 0 2 0 0 0 0 0 0
Cytoplasm 13 33 18 36 38 50 50 69 51 42 57 97 62 49 15 12 16 4 7 3 5 13 17
Endoplasmic Reticulum 9 21 1 1 2 10 2 2 5 1 7 40 33 9 5 3 8 0 5 2 2 7 17
Endosome 66 34 17 67 78 12 27 26 35 8 15 57 118 181 10 11 22 101 75 49 83 97 130
Golgi 32 25 2 4 1 0 0 0 0 0 0 9 17 16 30 7 15 67 43 26 44 92 123
Mitochondria 28 22 56 78 183 288 366 328 407 329 406 5 1 4 11 6 5 12 19 14 40 54 68
Nucleus 1 0 0 0 0 4 5 6 3 1 1 0 2 1 0 1 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 2 0 1 0 1 0 1 0 0 0 0 0 0 1 0 0 0 0 1
Nucleolus 1 0 1 2 2 4 9 27 8 8 9 0 0 0 1 0 1 0 0 0 0 1 0
Peroxisomes 2 0 6 28 40 8 27 23 50 21 37 2 7 22 0 0 0 21 10 14 13 14 21
SpindlePole 19 1 12 26 31 30 37 63 76 47 65 20 32 48 6 0 9 6 5 4 4 10 6
Vac/Vac Membrane 4 4 2 5 10 5 8 13 4 2 3 8 9 36 2 1 2 2 6 1 5 8 16
Unique Cell Count 160 143 97 193 312 362 460 460 539 393 522 281 283 311 77 40 72 243 216 129 224 355 479
Labelled Cell Count 212 183 129 261 411 453 572 594 699 514 675 351 337 387 114 51 93 243 216 129 224 355 479


Late Golgi

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.7 7.9 5.2 5.3 4.8 4.3 4.1 4.1 3.9 3.8 3.9 7.7 7.7 7.8 8.2 8.2 8.8 8.1 8.2 8.2
Std Deviation (1e-4) 1.3 1.2 1.3 1.4 1.2 1.2 1.5 1.3 1.1 1.0 1.2 1.2 1.1 1.3 1.1 1.2 1.5 1.4 1.4 1.4
Intensity Change (Log2) 0.03 -0.13 -0.27 -0.35 -0.34 -0.41 -0.47 -0.41 0.56 0.55 0.57 0.65 0.65 0.75 0.64 0.64 0.65


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 5.8 3.0 0 5.1 0 3.0
Bud -1.1 0 0 0 0 0 0 0 -1.5 0 0 -1.0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 -1.6 -1.2 -2.1 -0.9 -2.7 -2.1 -2.1 2.9 0.7 -0.7 0.2 1.5 0.6
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 3.6 3.2 0 0 0 0
Endosome 3.0 0 0 0 0 0 0 0 0.6 4.3 7.0 -0.8 1.3 2.0
Golgi 0 0 0 0 0 0 0 0 0 0 0 6.2 0 4.0
Mitochondria -2.8 0 0 0 0 0 0 0 -12.9 -13.7 -13.7 -5.8 -4.6 -6.8
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 2.1 0 0 0 0 0 0 0 0 0 0.3 0 0 0
SpindlePole 0.3 0 0 0 0 0 0 0 -1.6 -0.3 0.7 -1.0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 2.8 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.3915 3.0014 2.6326 2.411 2.4322 2.7212 2.4879 3.4675 2.9932 2.3001 2.4667 2.9849 0.9429 2.7125 1.929 2.084 1.39 1.8296
Actin 0.0367 0.0117 0.0429 0.0086 0.0487 0.0128 0.0131 0.0126 0.0147 0.1534 0.0606 0.0238 0.0151 0.0213 0.0118 0.0037 0.0024 0.0108
Bud 0.0005 0.0005 0.0007 0.0003 0.0015 0.0002 0.0005 0.0006 0.0004 0.0025 0.0001 0.0002 0.0001 0.0004 0.0001 0.0002 0.0001 0.0002
Bud Neck 0.0017 0.0007 0.0008 0.0025 0.0011 0.0012 0.002 0.0005 0.0008 0.0037 0.0004 0.0046 0.0011 0.0004 0.0004 0.0006 0.0003 0.0064
Bud Periphery 0.0009 0.0003 0.001 0.0004 0.0022 0.0002 0.0004 0.0003 0.0004 0.0031 0.0001 0.0005 0.0001 0.0002 0.0001 0.0001 0 0.0001
Bud Site 0.003 0.0104 0.0072 0.0038 0.0013 0.0005 0.0081 0.0239 0.0017 0.0097 0.0003 0.0006 0.0056 0.0031 0.0012 0.0004 0.0002 0.0003
Cell Periphery 0.0004 0.0001 0.0002 0.0006 0.0002 0 0.0002 0.0001 0.0005 0.0009 0 0.0004 0.0001 0 0 0 0 0
Cytoplasm 0.0282 0.0213 0.0239 0.0127 0.0126 0.0183 0.0279 0.0244 0.0217 0.0025 0.0062 0.0055 0.012 0.0437 0.0199 0.0027 0.0212 0.0106
Cytoplasmic Foci 0.2973 0.3221 0.2495 0.2604 0.2047 0.2756 0.3082 0.3289 0.2919 0.1816 0.297 0.2273 0.2898 0.3083 0.2743 0.3008 0.3494 0.2741
Eisosomes 0.001 0.0001 0.0003 0.0013 0.0003 0 0.0001 0.0001 0.0005 0.0011 0 0.0001 0 0 0 0 0 0
Endoplasmic Reticulum 0.0053 0.0009 0.0012 0.0018 0.0024 0.0024 0.0024 0.0019 0.0022 0.0016 0.0008 0.0022 0.0022 0.001 0.0016 0.0003 0.0009 0.0007
Endosome 0.2537 0.3129 0.2723 0.3681 0.2444 0.2415 0.2849 0.2472 0.1898 0.3088 0.3565 0.183 0.3695 0.2874 0.3145 0.2588 0.3512 0.2525
Golgi 0.1429 0.18 0.1865 0.2098 0.2059 0.231 0.1371 0.1919 0.1989 0.2124 0.1561 0.25 0.1875 0.2427 0.2727 0.3534 0.2033 0.2942
Lipid Particles 0.0437 0.0118 0.0148 0.0166 0.063 0.0331 0.076 0.0197 0.0195 0.0212 0.0186 0.0606 0.03 0.0148 0.0109 0.0062 0.0077 0.027
Mitochondria 0.0176 0.0064 0.0109 0.0075 0.0116 0.0092 0.0129 0.0029 0.0319 0.063 0.0022 0.0285 0.0127 0.0097 0.0044 0.0274 0.0018 0.007
None 0.0248 0.0029 0.0167 0.0042 0.05 0.0037 0.0017 0.0256 0.0532 0.0005 0.0015 0.0017 0.0008 0.0006 0.0014 0.0001 0.0003 0.0004
Nuclear Periphery 0.0177 0.0003 0.0022 0.0012 0.0038 0.0025 0.0017 0.0006 0.0084 0.0004 0.0004 0.0004 0.0011 0.0008 0.0006 0.0001 0.0003 0.0002
Nucleolus 0.0024 0.0001 0.0003 0.0019 0.0003 0.0002 0.0003 0.0001 0.0002 0.0002 0.0001 0.0001 0.0001 0 0.0001 0 0 0
Nucleus 0.0043 0.0002 0.0006 0.0012 0.0024 0.0003 0.0008 0.0002 0.0025 0.0003 0.0002 0.0003 0.004 0.0003 0.0002 0 0.0001 0.0001
Peroxisomes 0.1019 0.1125 0.1577 0.0917 0.1233 0.1628 0.1157 0.1134 0.1554 0.0287 0.0892 0.2058 0.0641 0.0607 0.0819 0.0435 0.0585 0.1127
Punctate Nuclear 0.007 0.0014 0.0049 0.0014 0.0022 0.0018 0.0024 0.0009 0.0009 0.0005 0.0006 0.0015 0.0008 0.0021 0.0009 0.0004 0.0006 0.0009
Vacuole 0.0054 0.0024 0.0042 0.0025 0.0126 0.0015 0.0023 0.0033 0.0026 0.002 0.0065 0.0018 0.0022 0.0014 0.002 0.0006 0.0009 0.0009
Vacuole Periphery 0.0039 0.001 0.0011 0.0013 0.0055 0.0011 0.0012 0.0008 0.002 0.0018 0.0023 0.001 0.001 0.0009 0.001 0.0007 0.0007 0.0008

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 21.9427 26.1539 11.0899 24.2195 21.6759 14.6204 25.4998 21.0352 17.6349 23.7761
Translational Efficiency 1.5421 1.1507 2.3804 0.8527 1.1232 1.9635 1.1361 1.383 1.4008 1.2341

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1216 1062 1359 1437 1707 2063 2141 1137 2923 3125 3500 2574

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 699.52 905.99 951.86 1043.72 720.36 857.59 978.35 1055.51 711.69 874.04 968.06 1048.93
Standard Deviation 87.50 128.27 137.39 132.91 107.33 112.47 128.99 134.81 100.09 120.28 132.94 133.88
Intensity Change Log 2 0.373130 0.444384 0.577298 0.251570 0.441633 0.551150 0.312738 0.442989 0.564091

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.002586 0.003182 0.019157 0.016819 0.002062 0.001631 0.014714 0.016634 0.002280 0.002158 0.016439 0.016738
Bud Neck 0.002011 0.001213 0.001115 0.004874 0.001971 0.001141 0.000836 0.003448 0.001987 0.001165 0.000945 0.004244
Bud Site 0.005660 0.006972 0.014763 0.042198 0.005206 0.003118 0.006743 0.030468 0.005395 0.004428 0.009857 0.037017
Cell Periphery 0.000125 0.000085 0.000055 0.000061 0.000057 0.000029 0.000025 0.000071 0.000085 0.000048 0.000036 0.000066
Cytoplasm 0.006857 0.009351 0.007067 0.088954 0.014628 0.006340 0.007593 0.037189 0.011395 0.007363 0.007389 0.066088
Cytoplasmic Foci 0.256593* 0.347995* 0.428124* 0.309776* 0.315341* 0.330628* 0.446754* 0.367339* 0.290901* 0.336530* 0.439520* 0.335203*
Eisosomes 0.000136 0.000054 0.000050 0.000047 0.000066 0.000044 0.000043 0.000068 0.000095 0.000048 0.000046 0.000056
Endoplasmic Reticulum 0.000344 0.000285 0.000940 0.000531 0.000295 0.000144 0.000255 0.000484 0.000316 0.000192 0.000521 0.000510
Endosome 0.101724 0.144208 0.159018 0.155296 0.192856 0.192176 0.195954 0.194717 0.154944 0.175874 0.181612 0.172709
Golgi 0.513417* 0.413919* 0.192634* 0.062496 0.382076* 0.389118* 0.166841* 0.093389 0.436715* 0.397546* 0.176856* 0.076142
Lipid Particles 0.021966 0.011601 0.013832 0.007644 0.018649 0.008203 0.009432 0.007521 0.020029 0.009358 0.011141 0.007590
Mitochondria 0.032024 0.011130 0.002835 0.004838 0.014162 0.014342 0.002012 0.007667 0.021593 0.013250 0.002331 0.006088
Mitotic Spindle 0.005862 0.014035 0.112289* 0.128059* 0.007358 0.014536 0.070431 0.071176 0.006736 0.014366 0.086684 0.102932*
None 0.000672 0.000218 0.000021 0.000087 0.000355 0.000073 0.000012 0.000034 0.000487 0.000122 0.000015 0.000064
Nuclear Periphery 0.000038 0.000030 0.000137 0.000377 0.000058 0.000046 0.000067 0.000125 0.000050 0.000041 0.000094 0.000265
Nuclear Periphery Foci 0.000254 0.000409 0.003050 0.001413 0.001038 0.000193 0.000859 0.001079 0.000712 0.000266 0.001710 0.001266
Nucleolus 0.000874 0.000136 0.000064 0.000232 0.000714 0.000108 0.000036 0.000110 0.000781 0.000117 0.000047 0.000178
Nucleus 0.001324 0.000252 0.000204 0.001302 0.001080 0.000367 0.000111 0.000627 0.001182 0.000328 0.000148 0.001004
Peroxisomes 0.043712 0.028172 0.032248 0.095903 0.035439 0.031930 0.066292 0.116535* 0.038880 0.030653 0.053073 0.105017*
Vacuole 0.001690 0.003846 0.011034 0.075663 0.004752 0.003768 0.010215 0.048814 0.003478 0.003794 0.010533 0.063803
Vacuole Periphery 0.002129 0.002908 0.001361 0.003431 0.001837 0.002066 0.000774 0.002506 0.001959 0.002352 0.001002 0.003022

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.80 -7.64 -6.82 -6.27 1.78 0.82 -8.48 -7.51 -7.76 -0.39 0.29 -11.40 -10.11 -10.14 0.89
Bud Neck 1.65 1.97 -7.32 -10.76 -12.57 2.37 3.50 -3.28 -5.22 -6.11 2.86 3.89 -8.11 -11.69 -13.51
Bud Site -1.01 -5.27 -14.14 -13.60 -9.56 2.37 -2.08 -10.84 -11.98 -10.17 1.31 -5.34 -17.92 -18.82 -14.88
Cell Periphery 1.32 4.08 3.49 0.71 -2.07 3.10 3.70 -1.23 -5.34 -6.28 3.00 5.56 1.88 -2.05 -7.71
Cytoplasm -1.55 -0.53 -20.74 -19.93 -20.60 4.39 3.26 -8.97 -11.80 -11.23 3.11 2.66 -21.50 -23.06 -22.97
Cytoplasmic Foci -13.36 -23.54 -1.34 10.75 20.09 -2.67 -21.67 -4.46 -2.49 12.57 -10.40 -31.74 -2.23 6.46 24.69
Eisosomes 5.14 5.45 5.63 1.33 0.81 3.30 4.06 0.07 -2.49 -2.85 6.13 6.69 4.99 -1.62 -2.26
Endoplasmic Reticulum 0.64 -3.86 -2.03 -3.23 2.58 2.18 0.24 -1.95 -3.99 -2.41 2.22 -3.05 -2.96 -5.62 0.27
Endosome -7.66 -10.88 -4.37 3.06 5.66 0.09 -0.37 1.67 1.65 2.13 -4.82 -6.32 1.04 5.59 7.02
Golgi 9.92 38.63* 63.32* 47.44* 26.59* -1.00 31.20 41.52* 46.27* 15.37* 6.09 47.80* 71.59* 68.77* 30.59*
Lipid Particles 3.51 3.02 5.69 1.69 3.99 5.42 4.81 5.64 0.44 1.81 6.56 5.63 8.05 1.32 3.96
Mitochondria 6.98 11.13 10.58 4.17 -1.01 -0.11 9.29 3.49 3.43 -2.85 4.91 14.37 10.53 5.87 -3.12
Mitotic Spindle -3.97 -17.82 -20.23 -18.24 -2.10 -3.83 -16.43 -12.49 -11.09 -0.94 -5.51 -24.13 -23.43 -21.23 -3.72
None 1.95 3.55 2.84 0.47 -4.49 2.40 3.00 2.73 0.92 -3.18 3.19 4.65 3.76 0.35 -5.25
Nuclear Periphery 0.46 -2.88 -5.77 -5.97 -4.17 0.60 -0.80 -3.68 -4.37 -3.47 0.65 -2.78 -6.47 -6.75 -5.27
Nuclear Periphery Foci -1.28 -5.49 -8.75 -7.00 2.38 2.89 0.29 -0.65 -5.18 -1.26 2.56 -3.82 -4.01 -9.44 0.82
Nucleolus 2.17 2.34 1.70 -2.37 -4.21 2.77 3.08 2.67 -0.68 -5.06 3.47 3.81 2.92 -2.51 -5.75
Nucleus 1.58 1.56 -2.30 -10.46 -10.69 1.59 2.32 0.06 -2.81 -5.80 2.20 2.68 -2.44 -9.15 -12.37
Peroxisomes 4.10 3.10 -7.63 -11.56 -10.86 1.35 -8.52 -12.93 -13.70 -7.05 3.71 -5.30 -15.00 -17.78 -10.60
Vacuole -4.94 -10.09 -24.06 -23.62 -21.85 1.47 -6.51 -14.35 -14.55 -12.78 -0.61 -11.04 -27.47 -27.35 -24.97
Vacuole Periphery -1.75 2.79 -1.07 0.38 -2.75 -0.73 5.36 -0.92 -0.42 -3.34 -1.53 5.90 -1.54 -0.40 -4.55