Standard name | |
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Description | GTPase-activating protein (RhoGAP) for Rho3p and Rho4p; possibly involved in control of actin cytoskeleton organization |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 21 | 34 | 3 | 6 | 12 | 7 | 12 | 23 | 8 | 9 | 7 | 15 | 24 | 31 | 23 | 10 | 14 | 6 | 28 | 35 | 7 | 34 | 61 | 65 |
Bud | 21 | 18 | 0 | 6 | 9 | 4 | 9 | 14 | 10 | 8 | 19 | 16 | 13 | 10 | 10 | 6 | 4 | 3 | 23 | 31 | 11 | 29 | 32 | 36 |
Bud Neck | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 2 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 19 | 29 | 7 | 26 | 34 | 20 |
Bud Site | 1 | 4 | 0 | 0 | 0 | 1 | 1 | 2 | 2 | 2 | 1 | 1 | 1 | 1 | 0 | 1 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 4 | 9 | 1 | 1 | 15 | 9 | 17 | 11 | 9 | 14 | 12 | 21 | 14 | 23 | 28 | 10 | 15 | 10 | 3 | 1 | 1 | 1 | 2 | 2 |
Cytoplasm | 130 | 185 | 20 | 39 | 148 | 125 | 206 | 209 | 157 | 180 | 133 | 153 | 211 | 322 | 359 | 100 | 93 | 67 | 118 | 151 | 31 | 64 | 113 | 162 |
Endoplasmic Reticulum | 10 | 24 | 0 | 0 | 3 | 6 | 9 | 10 | 12 | 8 | 5 | 9 | 5 | 8 | 4 | 11 | 6 | 7 | 7 | 9 | 2 | 2 | 7 | 11 |
Endosome | 28 | 6 | 0 | 1 | 6 | 4 | 6 | 3 | 4 | 2 | 4 | 5 | 9 | 3 | 9 | 13 | 5 | 11 | 9 | 8 | 3 | 10 | 10 | 13 |
Golgi | 0 | 1 | 0 | 0 | 1 | 0 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 2 | 6 | 5 | 5 | 0 | 4 | 0 | 0 | 0 | 0 |
Mitochondria | 17 | 5 | 0 | 2 | 4 | 18 | 35 | 40 | 49 | 53 | 128 | 99 | 2 | 0 | 0 | 7 | 3 | 2 | 8 | 3 | 7 | 8 | 5 | 6 |
Nucleus | 0 | 0 | 0 | 0 | 3 | 1 | 0 | 1 | 2 | 3 | 1 | 2 | 0 | 2 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Nucleolus | 3 | 0 | 0 | 0 | 1 | 2 | 0 | 2 | 1 | 1 | 1 | 0 | 0 | 1 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 7 | 6 | 0 | 0 | 5 | 2 | 3 | 2 | 5 | 3 | 2 | 4 | 3 | 4 | 4 | 9 | 8 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
SpindlePole | 2 | 0 | 0 | 1 | 9 | 7 | 4 | 15 | 6 | 8 | 2 | 3 | 9 | 10 | 13 | 15 | 11 | 8 | 2 | 1 | 0 | 10 | 2 | 3 |
Vac/Vac Membrane | 0 | 4 | 0 | 2 | 8 | 4 | 9 | 15 | 6 | 20 | 11 | 13 | 2 | 1 | 2 | 22 | 13 | 7 | 4 | 13 | 3 | 3 | 6 | 6 |
Unique Cell Count | 200 | 255 | 24 | 50 | 195 | 161 | 268 | 297 | 229 | 264 | 253 | 278 | 248 | 368 | 404 | 174 | 145 | 104 | 229 | 296 | 80 | 198 | 282 | 340 |
Labelled Cell Count | 244 | 297 | 24 | 58 | 225 | 191 | 313 | 350 | 271 | 313 | 327 | 342 | 293 | 418 | 454 | 215 | 178 | 127 | 229 | 296 | 80 | 198 | 282 | 340 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.6 | 8.2 | 8.8 | 7.4 | 8.6 | 7.2 | 6.8 | 6.9 | 6.2 | 6.0 | 5.4 | 5.7 | 8.6 | 8.6 | 8.4 | 9.9 | 11.0 | 9.8 | 8.1 | 8.3 | 8.4 |
Std Deviation (1e-4) | 0.9 | 1.2 | 1.6 | 1.4 | 1.9 | 1.7 | 1.8 | 1.5 | 2.0 | 1.6 | 1.5 | 1.5 | 1.3 | 1.3 | 1.4 | 2.2 | 3.4 | 2.1 | 1.1 | 1.3 | 1.5 |
Intensity Change (Log2) | – | – | – | -0.26 | -0.04 | -0.29 | -0.37 | -0.35 | -0.52 | -0.55 | -0.7 | -0.63 | -0.05 | -0.04 | -0.07 | 0.16 | 0.31 | 0.15 | -0.12 | -0.08 | -0.07 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.5 | -0.8 | -0.6 | -0.7 | -1.4 | -1.5 | -1.5 | -2.9 | -2.7 | 0.2 | 0.6 | 0.8 | -2.4 | -1.9 | -1.8 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.8 | 3.6 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |