Standard name
Human Ortholog
Description GTPase-activating protein (RhoGAP) for Rho3p and Rho4p; possibly involved in control of actin cytoskeleton organization
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.1 0.13 0.12 0.12 0.06 0 0 0.08 0 0 0 0.05 0.1 0.08 0.06 0.06 0.1 0.06 0.12 0.12 0.09 0.17 0.22 0.19
Bud 0.1 0.07 0 0.12 0.05 0 0 0.05 0 0 0.08 0.06 0.05 0 0 0 0 0 0.1 0.11 0.15 0.15 0.12 0.11
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.1 0.09 0.13 0.12 0.06
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0.08 0.06 0.06 0 0 0.05 0.05 0.08 0.06 0.06 0.07 0.06 0.1 0.1 0 0 0 0 0 0
Cytoplasm 0.65 0.73 0.83 0.78 0.76 0.78 0.77 0.7 0.69 0.68 0.53 0.55 0.85 0.88 0.89 0.57 0.64 0.64 0.52 0.51 0.39 0.32 0.4 0.48
Endoplasmic Reticulum 0.05 0.09 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0.06 0 0.07 0 0 0 0 0 0
Endosome 0.14 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0.11 0 0 0.05 0.06 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Mitochondria 0.09 0 0 0 0 0.11 0.13 0.13 0.21 0.2 0.51 0.36 0 0 0 0 0 0 0 0 0.09 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0.05 0 0 0.05 0 0 0 0 0 0 0 0.09 0.08 0.08 0 0 0 0.05 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.05 0 0.08 0 0.05 0 0 0 0.13 0.09 0.07 0 0.05 0.05 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 21 34 3 6 12 7 12 23 8 9 7 15 24 31 23 10 14 6 28 35 7 34 61 65
Bud 21 18 0 6 9 4 9 14 10 8 19 16 13 10 10 6 4 3 23 31 11 29 32 36
Bud Neck 0 1 0 0 1 1 0 2 0 1 1 1 0 0 0 1 0 0 19 29 7 26 34 20
Bud Site 1 4 0 0 0 1 1 2 2 2 1 1 1 1 0 1 0 0 – – – – – –
Cell Periphery 4 9 1 1 15 9 17 11 9 14 12 21 14 23 28 10 15 10 3 1 1 1 2 2
Cytoplasm 130 185 20 39 148 125 206 209 157 180 133 153 211 322 359 100 93 67 118 151 31 64 113 162
Endoplasmic Reticulum 10 24 0 0 3 6 9 10 12 8 5 9 5 8 4 11 6 7 7 9 2 2 7 11
Endosome 28 6 0 1 6 4 6 3 4 2 4 5 9 3 9 13 5 11 9 8 3 10 10 13
Golgi 0 1 0 0 1 0 2 0 0 1 0 0 0 2 2 6 5 5 0 4 0 0 0 0
Mitochondria 17 5 0 2 4 18 35 40 49 53 128 99 2 0 0 7 3 2 8 3 7 8 5 6
Nucleus 0 0 0 0 3 1 0 1 2 3 1 2 0 2 0 1 1 1 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Nucleolus 3 0 0 0 1 2 0 2 1 1 1 0 0 1 0 3 0 0 0 0 0 0 0 0
Peroxisomes 7 6 0 0 5 2 3 2 5 3 2 4 3 4 4 9 8 0 0 0 0 0 0 1
SpindlePole 2 0 0 1 9 7 4 15 6 8 2 3 9 10 13 15 11 8 2 1 0 10 2 3
Vac/Vac Membrane 0 4 0 2 8 4 9 15 6 20 11 13 2 1 2 22 13 7 4 13 3 3 6 6
Unique Cell Count 200 255 24 50 195 161 268 297 229 264 253 278 248 368 404 174 145 104 229 296 80 198 282 340
Labelled Cell Count 244 297 24 58 225 191 313 350 271 313 327 342 293 418 454 215 178 127 229 296 80 198 282 340


Ambiguous, Bud, Bud Neck, Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.6 8.2 8.8 7.4 8.6 7.2 6.8 6.9 6.2 6.0 5.4 5.7 8.6 8.6 8.4 9.9 11.0 9.8 8.1 8.3 8.4
Std Deviation (1e-4) 0.9 1.2 1.6 1.4 1.9 1.7 1.8 1.5 2.0 1.6 1.5 1.5 1.3 1.3 1.4 2.2 3.4 2.1 1.1 1.3 1.5
Intensity Change (Log2) – – – -0.26 -0.04 -0.29 -0.37 -0.35 -0.52 -0.55 -0.7 -0.63 -0.05 -0.04 -0.07 0.16 0.31 0.15 -0.12 -0.08 -0.07


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.5 -0.8 -0.6 -0.7 -1.4 -1.5 -1.5 -2.9 -2.7 0.2 0.6 0.8 -2.4 -1.9 -1.8
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 4.8 3.6 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.0926 2.3539 1.9086 1.8532 1.9278 2.3542 2.7467 3.0405 2.8226 2.765 2.441 2.8246 2.8332 2.6499 2.4664 2.6809 2.1789 2.5514
Actin 0.0963 0.0423 0.0949 0.0966 0.1226 0.0506 0.0884 0.0156 0.078 0.0458 0.0751 0.037 0.0723 0.0334 0.1061 0.0672 0.0656 0.0633
Bud 0.0079 0.0167 0.1546 0.0239 0.0098 0.002 0.0396 0.0272 0.1964 0.0449 0.0393 0.0094 0.0191 0.015 0.1497 0.03 0.0052 0.0055
Bud Neck 0.0257 0.0052 0.0042 0.0043 0.0012 0.408 0.0196 0.0035 0.0013 0.0038 0.0332 0.3979 0.0355 0.0082 0.0075 0.0286 0.1397 0.4065
Bud Periphery 0.0107 0.0181 0.2453 0.0454 0.0062 0.0033 0.0324 0.0158 0.2797 0.1378 0.0444 0.0098 0.0399 0.014 0.209 0.0679 0.0059 0.0071
Bud Site 0.0713 0.2229 0.0479 0.0042 0.0044 0.0033 0.0836 0.3258 0.0575 0.0158 0.0253 0.0073 0.0957 0.2545 0.0454 0.0082 0.0363 0.0091
Cell Periphery 0.0058 0.0066 0.0072 0.0027 0.0012 0.002 0.0068 0.0043 0.0031 0.0049 0.0019 0.0016 0.0052 0.0026 0.0041 0.004 0.0012 0.0012
Cytoplasm 0.4562 0.5537 0.244 0.6547 0.2976 0.3938 0.4307 0.5276 0.2995 0.5437 0.4805 0.4329 0.4439 0.5413 0.3441 0.607 0.5747 0.3427
Cytoplasmic Foci 0.0891 0.0459 0.0281 0.021 0.1326 0.0411 0.0799 0.0303 0.0201 0.0546 0.1093 0.0588 0.1001 0.0399 0.0244 0.034 0.0686 0.0677
Eisosomes 0.0005 0.0004 0.0004 0.0003 0.0022 0.0001 0.0007 0.0001 0.0002 0.0002 0.0002 0.0001 0.0005 0.0001 0.0002 0.0002 0.0005 0.0001
Endoplasmic Reticulum 0.0147 0.0076 0.0069 0.0049 0.0094 0.0056 0.0129 0.0028 0.0028 0.0056 0.0026 0.0022 0.0113 0.004 0.0052 0.0051 0.0023 0.0031
Endosome 0.0625 0.0162 0.0454 0.037 0.1331 0.0309 0.0632 0.0127 0.0084 0.0347 0.0322 0.0089 0.0363 0.0136 0.0157 0.0234 0.013 0.0144
Golgi 0.0473 0.0082 0.018 0.0132 0.0399 0.0169 0.0264 0.0059 0.007 0.0127 0.0254 0.0049 0.021 0.007 0.008 0.0145 0.0047 0.005
Lipid Particles 0.0241 0.0038 0.0174 0.006 0.0526 0.0037 0.0099 0.0004 0.0022 0.031 0.0302 0.0008 0.0144 0.0007 0.0024 0.0163 0.0018 0.0015
Mitochondria 0.016 0.0016 0.0242 0.0233 0.03 0.0038 0.0295 0.0017 0.0189 0.0079 0.0064 0.0017 0.0124 0.0012 0.0025 0.0033 0.0009 0.0006
None 0.0311 0.0414 0.0381 0.0485 0.1214 0.0276 0.0364 0.0173 0.0104 0.028 0.0083 0.0163 0.0473 0.0511 0.0538 0.0554 0.0483 0.046
Nuclear Periphery 0.0075 0.0006 0.0025 0.0005 0.0019 0.0008 0.0039 0.0004 0.0006 0.0013 0.0069 0.0003 0.0085 0.0006 0.0007 0.0015 0.0008 0.0115
Nucleolus 0.0006 0.0003 0.0013 0.0001 0.0007 0.0002 0.0011 0.0003 0.0002 0.0003 0.0148 0.0001 0.0007 0.0003 0.0003 0.0006 0.0038 0.0002
Nucleus 0.0022 0.001 0.0017 0.0007 0.0009 0.0006 0.0043 0.0014 0.0009 0.0011 0.0182 0.001 0.0034 0.0014 0.0009 0.0018 0.0052 0.0004
Peroxisomes 0.0177 0.0024 0.0048 0.0037 0.0206 0.0023 0.0139 0.0008 0.0063 0.016 0.0219 0.0051 0.0209 0.0044 0.0141 0.0135 0.0034 0.012
Punctate Nuclear 0.0039 0.001 0.0012 0.0003 0.0019 0.0011 0.0038 0.0016 0.0015 0.0018 0.0192 0.0015 0.0042 0.0014 0.0018 0.0114 0.0151 0.0005
Vacuole 0.0075 0.0037 0.0099 0.0074 0.0074 0.0019 0.01 0.0043 0.0043 0.0066 0.0034 0.002 0.0063 0.005 0.0036 0.005 0.0024 0.0011
Vacuole Periphery 0.0015 0.0003 0.0021 0.0012 0.0024 0.0005 0.0029 0.0005 0.001 0.0016 0.0013 0.0005 0.0012 0.0005 0.0006 0.0008 0.0005 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 22.3328 17.253 12.0709 18.4539 14.299 14.8803 18.9397 16.9879 19.4911 21.2838
Translational Efficiency 0.8882 0.9119 1.4604 0.8003 1.0146 1.3052 0.9662 1.2266 1.171 1.0175

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description GTPase-activating protein (RhoGAP) for Rho3p and Rho4p; possibly involved in control of actin cytoskeleton organization
Localization
Cell Percentages cytoplasm (81%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-10

Rgd1

Rgd1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rgd1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available