Standard name
Description GTPase-activating protein (RhoGAP) for Rho3p and Rho4p; possibly involved in control of actin cytoskeleton organization
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.1 0.13 0.12 0.12 0.06 0 0 0.08 0 0 0 0.05 0.1 0.08 0.06 0.06 0.1 0.06 0.12 0.12 0.09 0.17 0.22 0.19
Bud 0.1 0.07 0 0.12 0.05 0 0 0.05 0 0 0.08 0.06 0.05 0 0 0 0 0 0.1 0.11 0.15 0.15 0.12 0.11
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.1 0.09 0.13 0.12 0.06
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0.08 0.06 0.06 0 0 0.05 0.05 0.08 0.06 0.06 0.07 0.06 0.1 0.1 0 0 0 0 0 0
Cytoplasm 0.65 0.73 0.83 0.78 0.76 0.78 0.77 0.7 0.69 0.68 0.53 0.55 0.85 0.88 0.89 0.57 0.64 0.64 0.52 0.51 0.39 0.32 0.4 0.48
Endoplasmic Reticulum 0.05 0.09 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0.06 0 0.07 0 0 0 0 0 0
Endosome 0.14 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0.11 0 0 0.05 0.06 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Mitochondria 0.09 0 0 0 0 0.11 0.13 0.13 0.21 0.2 0.51 0.36 0 0 0 0 0 0 0 0 0.09 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0.05 0 0 0.05 0 0 0 0 0 0 0 0.09 0.08 0.08 0 0 0 0.05 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.05 0 0.08 0 0.05 0 0 0 0.13 0.09 0.07 0 0.05 0.05 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 21 34 3 6 12 7 12 23 8 9 7 15 24 31 23 10 14 6 28 35 7 34 61 65
Bud 21 18 0 6 9 4 9 14 10 8 19 16 13 10 10 6 4 3 23 31 11 29 32 36
Bud Neck 0 1 0 0 1 1 0 2 0 1 1 1 0 0 0 1 0 0 19 29 7 26 34 20
Bud Site 1 4 0 0 0 1 1 2 2 2 1 1 1 1 0 1 0 0 – – – – – –
Cell Periphery 4 9 1 1 15 9 17 11 9 14 12 21 14 23 28 10 15 10 3 1 1 1 2 2
Cytoplasm 130 185 20 39 148 125 206 209 157 180 133 153 211 322 359 100 93 67 118 151 31 64 113 162
Endoplasmic Reticulum 10 24 0 0 3 6 9 10 12 8 5 9 5 8 4 11 6 7 7 9 2 2 7 11
Endosome 28 6 0 1 6 4 6 3 4 2 4 5 9 3 9 13 5 11 9 8 3 10 10 13
Golgi 0 1 0 0 1 0 2 0 0 1 0 0 0 2 2 6 5 5 0 4 0 0 0 0
Mitochondria 17 5 0 2 4 18 35 40 49 53 128 99 2 0 0 7 3 2 8 3 7 8 5 6
Nucleus 0 0 0 0 3 1 0 1 2 3 1 2 0 2 0 1 1 1 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Nucleolus 3 0 0 0 1 2 0 2 1 1 1 0 0 1 0 3 0 0 0 0 0 0 0 0
Peroxisomes 7 6 0 0 5 2 3 2 5 3 2 4 3 4 4 9 8 0 0 0 0 0 0 1
SpindlePole 2 0 0 1 9 7 4 15 6 8 2 3 9 10 13 15 11 8 2 1 0 10 2 3
Vac/Vac Membrane 0 4 0 2 8 4 9 15 6 20 11 13 2 1 2 22 13 7 4 13 3 3 6 6
Unique Cell Count 200 255 24 50 195 161 268 297 229 264 253 278 248 368 404 174 145 104 229 296 80 198 282 340
Labelled Cell Count 244 297 24 58 225 191 313 350 271 313 327 342 293 418 454 215 178 127 229 296 80 198 282 340


Ambiguous, Bud, Bud Neck, Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.6 8.2 8.8 7.4 8.6 7.2 6.8 6.9 6.2 6.0 5.4 5.7 8.6 8.6 8.4 9.9 11.0 9.8 8.1 8.3 8.4
Std Deviation (1e-4) 0.9 1.2 1.6 1.4 1.9 1.7 1.8 1.5 2.0 1.6 1.5 1.5 1.3 1.3 1.4 2.2 3.4 2.1 1.1 1.3 1.5
Intensity Change (Log2) – – – -0.26 -0.04 -0.29 -0.37 -0.35 -0.52 -0.55 -0.7 -0.63 -0.05 -0.04 -0.07 0.16 0.31 0.15 -0.12 -0.08 -0.07


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.5 -0.8 -0.6 -0.7 -1.4 -1.5 -1.5 -2.9 -2.7 0.2 0.6 0.8 -2.4 -1.9 -1.8
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 4.8 3.6 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0