Standard name
Description Mitochondrial serine hydroxymethyltransferase; converts serine to glycine plus 5,10 methylenetetrahydrofolate; involved in generating precursors for purine, pyrimidine, amino acid, and lipid biosynthesis; reverse reaction generates serine

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.14 0 0 0 0.05 0.05 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Golgi 0 0.1 0.41 0.14 0.3 0.05 0.05 0 0 0 0 0.23 0.35 0.48 0.42 0.44 0.31 0 0.11 0 0.16 0.18 0.21
Mitochondria 0.9 0.85 0.57 0.89 0.74 0.82 0.79 0.82 0.87 0.91 0.86 0.85 0.79 0.7 0.2 0.15 0.1 0.79 0.73 0.8 0.64 0.57 0.52
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0.08 0.08 0.09 0.06 0 0.05 0 0 0 0.05 0 0 0 0 0 0 0 0
Nucleolus 0.08 0.05 0.09 0 0.07 0.14 0.18 0.16 0.11 0.12 0.12 0 0 0 0.15 0.13 0.09 0 0 0 0 0 0
Peroxisomes 0.07 0 0.05 0.05 0 0 0 0 0 0 0 0 0.05 0.07 0.07 0.07 0.11 0.06 0 0.07 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.05 0.1 0.18 0 0.1 0.07 0 0.06 0.05 0 0 0.15 0.08 0.07 0.13 0.17 0.23 0 0 0 0.05 0.08 0.11
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 2 1 6 5 2 6 1 3 0 1 5 5 2 3 4 12 8 9 4 3 6 3
Bud 5 3 2 1 4 3 4 4 4 10 4 1 0 0 1 2 4 1 0 1 0 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 1 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 3
Cell Periphery 2 3 4 7 2 1 1 3 2 1 2 5 3 5 2 3 5 0 1 0 0 0 0
Cytoplasm 1 1 0 3 10 3 13 4 4 8 6 2 8 4 5 3 7 0 0 0 0 0 0
Endoplasmic Reticulum 0 2 0 0 1 2 0 3 2 0 4 0 0 0 2 1 0 0 0 0 0 0 0
Endosome 4 1 5 3 4 7 5 5 5 1 1 0 2 2 1 2 1 6 6 5 0 5 6
Golgi 2 19 66 26 78 20 24 13 19 4 20 38 77 113 36 38 27 13 26 8 10 24 27
Mitochondria 258 161 91 166 193 330 366 288 388 364 471 140 173 164 17 13 9 238 172 165 44 79 69
Nucleus 0 0 1 0 2 0 2 1 2 0 1 0 1 0 1 0 1 0 0 0 0 1 0
Nuclear Periphery 11 2 4 1 5 32 35 30 29 14 26 5 1 5 4 1 1 3 1 1 0 1 0
Nucleolus 24 9 14 5 19 57 84 57 47 48 64 5 6 3 13 11 8 0 2 0 1 1 0
Peroxisomes 20 7 8 10 9 4 9 1 11 6 5 3 10 16 6 6 10 18 6 14 0 3 3
SpindlePole 2 1 3 0 1 1 0 4 1 1 6 0 1 2 2 0 1 2 1 1 0 0 2
Vac/Vac Membrane 14 18 29 5 25 29 14 20 22 6 21 24 17 17 11 15 20 4 5 1 3 10 14
Unique Cell Count 286 189 160 187 262 404 465 352 447 402 547 164 220 233 86 86 88 300 238 208 69 139 132
Labelled Cell Count 344 229 228 233 358 491 563 434 539 464 632 228 304 333 104 99 109 300 238 208 69 139 132


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 13.2 18.5 16.7 17.8 19.9 18.6 18.9 17.7 17.5 16.7 17.1 18.9 19.1 18.1 21.8 23.9 22.0 13.8 18.7 19.7
Std Deviation (1e-4) 3.4 4.5 3.5 4.1 5.2 4.4 5.0 4.9 5.0 5.3 5.1 5.2 4.3 3.8 6.1 7.6 6.8 3.8 5.5 6.4
Intensity Change (Log2) 0.09 0.25 0.16 0.18 0.08 0.07 0.0 0.04 0.18 0.19 0.12 0.39 0.52 0.4 -0.27 0.17 0.24


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.7 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi -5.8 -2.4 -10.8 -11.2 -10.9 -11.6 -13.0 -12.8 -3.5 -1.2 1.4 0.1 0.4 -1.6
Mitochondria 6.8 3.6 6.1 5.4 6.0 8.0 9.2 8.1 5.7 4.5 2.8 -5.6 -6.3 -7.2
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 2.4 2.3 2.5 1.9 0.6 1.2 0 0 0 0 0 0
Nucleolus -2.5 -0.6 1.7 2.8 2.3 0.6 1.1 1.0 -2.2 -2.6 -3.6 1.5 1.0 0.1
Peroxisomes 0.1 -0.8 0 0 0 -1.6 0 0 -1.6 -0.2 0.8 0 0 1.8
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -4.8 -2.6 -3.9 -6.5 -4.4 -5.2 -7.4 -6.2 -0.8 -3.1 -3.3 -1.1 -0.1 0.9


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Shm1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available