Standard name
Description 3-ketosphinganine reductase; catalyzes the second step in phytosphingosine synthesis; essential for growth in the absence of exogenous dihydrosphingosine or phytosphingosine; localized to lipid droplets; member of short chain dehydrogenase/reductase protein family
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.07 0.05 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 – 0.12 0.1 0.06 0 0.07 0.05 0 0.06
Bud 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 – 0.06 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0.05 0 0 0.05 0 0 0 0 0 0 0 0.11 0.05 0.05 – 0 0.05 0 0 0 0 0 0
Cytoplasm 0.21 0.47 0.41 0.46 0.55 0.44 0.31 0.25 0.16 0.11 0.1 0.09 0.46 0.65 0.63 – 0.41 0.64 0.06 0.18 0 0.07 0.13 0.15
Endoplasmic Reticulum 0.68 0.5 0.41 0.33 0.14 0.22 0.15 0.11 0.14 0.08 0.13 0.06 0.49 0.28 0.34 – 0.06 0 0.69 0.56 0.71 0.46 0.48 0.46
Endosome 0 0 0 0.06 0 0 0 0.08 0 0.08 0 0 0 0 0 – 0.12 0.05 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.06 0 0 0 0 0 0 0
Mitochondria 0.09 0 0.14 0.19 0.33 0.43 0.52 0.5 0.67 0.65 0.78 0.79 0 0 0 – 0.18 0.05 0.06 0.06 0.1 0.16 0.15 0.1
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.06 0.05 0 0 0 0 0 0
Vac/Vac Membrane 0 0.05 0.06 0.06 0.06 0.06 0.15 0.14 0.13 0.16 0.09 0.08 0 0 0 – 0.06 0.08 0 0.05 0 0.15 0.12 0.13
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 5 7 7 4 8 13 1 6 9 11 9 7 2 10 9 – 2 4 5 5 12 3 7 12
Bud 0 0 3 2 33 22 22 28 26 19 21 31 1 1 2 – 1 1 0 0 0 0 3 1
Bud Neck 1 0 1 0 0 0 0 0 0 0 0 0 0 2 1 – 0 1 1 2 0 1 4 3
Bud Site 0 0 0 0 0 0 1 0 5 6 1 6 0 0 0 – 0 0 – – – – – –
Cell Periphery 2 7 3 3 29 10 21 10 3 1 5 2 4 10 13 – 0 2 0 0 0 1 5 6
Cytoplasm 14 63 67 115 330 223 275 169 100 98 57 78 17 131 160 – 7 25 5 28 8 4 30 30
Endoplasmic Reticulum 46 67 66 83 87 114 134 73 84 69 72 51 18 57 85 – 1 0 60 87 137 29 107 91
Endosome 0 3 7 14 26 8 34 54 26 68 12 23 0 2 6 – 2 2 2 4 3 1 2 3
Golgi 0 3 0 0 0 0 0 0 0 0 1 1 0 0 0 – 1 0 0 4 1 0 1 1
Mitochondria 6 3 22 47 198 219 466 334 415 567 447 700 0 0 0 – 3 2 5 9 19 10 34 19
Nucleus 0 1 0 2 0 1 8 6 4 9 4 5 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 2 6 15 4 9 6 15 10 0 0 0 – 0 0 2 0 0 0 0 0
Nucleolus 0 0 0 0 1 0 3 3 6 20 8 42 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 2 1 0 0 2 3 4 2 2 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 1 2 2 16 6 21 1 13 0 1 6 – 1 2 0 0 0 0 0 0
Vac/Vac Membrane 0 7 10 16 39 30 135 91 82 138 52 71 0 1 2 – 1 3 3 7 7 9 28 26
Unique Cell Count 68 134 162 252 605 511 892 669 615 870 571 882 37 201 253 17 39 88 155 194 65 226 199
Labelled Cell Count 74 161 186 288 755 648 1117 796 778 1037 707 1042 42 215 284 19 42 88 155 194 65 226 199


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.5 7.1 5.5 5.7 5.4 5.2 4.9 5.5 5.4 5.3 5.6 5.5 6.6 6.6 6.6 – 7.6 6.8 7.9 7.2 7.8
Std Deviation (1e-4) 0.8 1.3 1.2 1.4 1.4 1.4 1.2 1.5 1.2 1.4 1.2 1.2 0.6 1.1 1.0 – 2.4 1.4 1.1 1.2 1.5
Intensity Change (Log2) – – – 0.05 -0.02 -0.07 -0.16 -0.0 -0.02 -0.03 0.02 -0.0 0.26 0.27 0.26 – 0.47 0.32 0.53 0.4 0.52


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0.3 -0.4 – 0 0
Bud 0 1.9 1.4 0 1.4 1.4 0 1.2 1.1 0 0 0 – 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Cell Periphery 0 1.7 0 0 0 0 0 0 0 0 1.6 1.7 – 0 0
Cytoplasm 0.9 3.0 0.5 -2.6 -4.1 -6.9 -9.6 -9.4 -11.0 0.5 4.5 4.4 – 0 2.6
Endoplasmic Reticulum -1.6 -7.5 -4.6 -7.7 -9.1 -7.8 -11.4 -8.1 -13.0 0.9 -2.5 -1.5 – -2.8 -4.9
Endosome 0.6 0 0 -0.3 1.6 -0.1 1.6 0 0 0 0 -1.1 – 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Mitochondria 1.4 4.8 6.8 9.1 8.4 12.3 12.2 15.1 16.7 0 -5.4 -6.0 – 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Nucleolus 0 0 0 0 0 0 0 0 2.8 0 0 0 – 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Vacuole 0.1 0.1 -0.1 3.0 2.6 2.5 3.2 1.2 0.8 0 0 0 – 0 0