Standard name
Human Ortholog
Description 3-ketosphinganine reductase; catalyzes the second step in phytosphingosine synthesis; essential for growth in the absence of exogenous dihydrosphingosine or phytosphingosine; localized to lipid droplets; member of short chain dehydrogenase/reductase protein family
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.07 0.05 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 – 0.12 0.1 0.06 0 0.07 0.05 0 0.06
Bud 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 – 0.06 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0.05 0 0 0.05 0 0 0 0 0 0 0 0.11 0.05 0.05 – 0 0.05 0 0 0 0 0 0
Cytoplasm 0.21 0.47 0.41 0.46 0.55 0.44 0.31 0.25 0.16 0.11 0.1 0.09 0.46 0.65 0.63 – 0.41 0.64 0.06 0.18 0 0.07 0.13 0.15
Endoplasmic Reticulum 0.68 0.5 0.41 0.33 0.14 0.22 0.15 0.11 0.14 0.08 0.13 0.06 0.49 0.28 0.34 – 0.06 0 0.69 0.56 0.71 0.46 0.48 0.46
Endosome 0 0 0 0.06 0 0 0 0.08 0 0.08 0 0 0 0 0 – 0.12 0.05 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.06 0 0 0 0 0 0 0
Mitochondria 0.09 0 0.14 0.19 0.33 0.43 0.52 0.5 0.67 0.65 0.78 0.79 0 0 0 – 0.18 0.05 0.06 0.06 0.1 0.16 0.15 0.1
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.06 0.05 0 0 0 0 0 0
Vac/Vac Membrane 0 0.05 0.06 0.06 0.06 0.06 0.15 0.14 0.13 0.16 0.09 0.08 0 0 0 – 0.06 0.08 0 0.05 0 0.15 0.12 0.13
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 5 7 7 4 8 13 1 6 9 11 9 7 2 10 9 – 2 4 5 5 12 3 7 12
Bud 0 0 3 2 33 22 22 28 26 19 21 31 1 1 2 – 1 1 0 0 0 0 3 1
Bud Neck 1 0 1 0 0 0 0 0 0 0 0 0 0 2 1 – 0 1 1 2 0 1 4 3
Bud Site 0 0 0 0 0 0 1 0 5 6 1 6 0 0 0 – 0 0 – – – – – –
Cell Periphery 2 7 3 3 29 10 21 10 3 1 5 2 4 10 13 – 0 2 0 0 0 1 5 6
Cytoplasm 14 63 67 115 330 223 275 169 100 98 57 78 17 131 160 – 7 25 5 28 8 4 30 30
Endoplasmic Reticulum 46 67 66 83 87 114 134 73 84 69 72 51 18 57 85 – 1 0 60 87 137 29 107 91
Endosome 0 3 7 14 26 8 34 54 26 68 12 23 0 2 6 – 2 2 2 4 3 1 2 3
Golgi 0 3 0 0 0 0 0 0 0 0 1 1 0 0 0 – 1 0 0 4 1 0 1 1
Mitochondria 6 3 22 47 198 219 466 334 415 567 447 700 0 0 0 – 3 2 5 9 19 10 34 19
Nucleus 0 1 0 2 0 1 8 6 4 9 4 5 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 2 6 15 4 9 6 15 10 0 0 0 – 0 0 2 0 0 0 0 0
Nucleolus 0 0 0 0 1 0 3 3 6 20 8 42 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 2 1 0 0 2 3 4 2 2 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 1 2 2 16 6 21 1 13 0 1 6 – 1 2 0 0 0 0 0 0
Vac/Vac Membrane 0 7 10 16 39 30 135 91 82 138 52 71 0 1 2 – 1 3 3 7 7 9 28 26
Unique Cell Count 68 134 162 252 605 511 892 669 615 870 571 882 37 201 253 17 39 88 155 194 65 226 199
Labelled Cell Count 74 161 186 288 755 648 1117 796 778 1037 707 1042 42 215 284 19 42 88 155 194 65 226 199


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.5 7.1 5.5 5.7 5.4 5.2 4.9 5.5 5.4 5.3 5.6 5.5 6.6 6.6 6.6 – 7.6 6.8 7.9 7.2 7.8
Std Deviation (1e-4) 0.8 1.3 1.2 1.4 1.4 1.4 1.2 1.5 1.2 1.4 1.2 1.2 0.6 1.1 1.0 – 2.4 1.4 1.1 1.2 1.5
Intensity Change (Log2) – – – 0.05 -0.02 -0.07 -0.16 -0.0 -0.02 -0.03 0.02 -0.0 0.26 0.27 0.26 – 0.47 0.32 0.53 0.4 0.52


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0.3 -0.4 – 0 0
Bud 0 1.9 1.4 0 1.4 1.4 0 1.2 1.1 0 0 0 – 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Cell Periphery 0 1.7 0 0 0 0 0 0 0 0 1.6 1.7 – 0 0
Cytoplasm 0.9 3.0 0.5 -2.6 -4.1 -6.9 -9.6 -9.4 -11.0 0.5 4.5 4.4 – 0 2.6
Endoplasmic Reticulum -1.6 -7.5 -4.6 -7.7 -9.1 -7.8 -11.4 -8.1 -13.0 0.9 -2.5 -1.5 – -2.8 -4.9
Endosome 0.6 0 0 -0.3 1.6 -0.1 1.6 0 0 0 0 -1.1 – 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Mitochondria 1.4 4.8 6.8 9.1 8.4 12.3 12.2 15.1 16.7 0 -5.4 -6.0 – 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Nucleolus 0 0 0 0 0 0 0 0 2.8 0 0 0 – 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Vacuole 0.1 0.1 -0.1 3.0 2.6 2.5 3.2 1.2 0.8 0 0 0 – 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.8342 3.6419 3.4452 3.5223 2.7821 3.6653 3.0929 3.5321 3.1172 2.7201 2.9836 3.1971 1.6235 2.0981 1.783 1.904 1.4279 1.927
Actin 0.0144 0.0081 0.0123 0.0406 0.0149 0.0044 0.0086 0.0106 0.0219 0.0031 0.0116 0.0069 0.021 0.0155 0.0064 0.0019 0.0029 0.01
Bud 0.002 0.0033 0.0018 0.0018 0.0055 0.0058 0.0001 0.0006 0.0004 0.0001 0.0002 0.0001 0.0003 0.0002 0.0002 0.0002 0.0001 0.0002
Bud Neck 0.0044 0.0037 0.0094 0.024 0.0032 0.0101 0.0014 0.0025 0.0027 0.0008 0.0262 0.0032 0.0036 0.0005 0.0029 0.003 0.0013 0.0102
Bud Periphery 0.0018 0.0017 0.0021 0.0024 0.005 0.0047 0.0003 0.001 0.0009 0.0004 0.0006 0.0003 0.0006 0.0002 0.0004 0.0004 0.0001 0.0005
Bud Site 0.016 0.0521 0.0176 0.0076 0.0051 0.0054 0.0015 0.0421 0.0058 0.0004 0.003 0.0004 0.0032 0.0028 0.0052 0.0044 0.0005 0.0005
Cell Periphery 0.0031 0.0037 0.0035 0.0018 0.0025 0.003 0.0107 0.0094 0.0124 0.0046 0.0063 0.0048 0.0081 0.0058 0.0056 0.0026 0.002 0.0044
Cytoplasm 0.1483 0.1987 0.1926 0.1543 0.2545 0.2462 0.1781 0.2859 0.2185 0.1594 0.2095 0.2748 0.1573 0.2416 0.2034 0.1808 0.1798 0.1692
Cytoplasmic Foci 0.0796 0.08 0.0681 0.0515 0.0895 0.0717 0.0617 0.0457 0.0479 0.0766 0.0612 0.0396 0.0669 0.0752 0.0549 0.0401 0.062 0.0511
Eisosomes 0.0003 0.0002 0.0005 0.0003 0.0004 0.0002 0.0023 0.0004 0.0008 0.0002 0.0006 0.0002 0.0008 0.0001 0.0004 0.0001 0.0001 0.0001
Endoplasmic Reticulum 0.229 0.2623 0.2271 0.2965 0.1722 0.2363 0.2299 0.2591 0.3603 0.3906 0.3316 0.3326 0.2787 0.2172 0.32 0.5138 0.458 0.4089
Endosome 0.1529 0.1442 0.1536 0.1251 0.1704 0.1333 0.063 0.0824 0.0756 0.1435 0.0946 0.0521 0.0875 0.0767 0.094 0.1103 0.1216 0.0863
Golgi 0.0289 0.0186 0.0307 0.0421 0.0439 0.0176 0.0254 0.0215 0.0257 0.0358 0.026 0.0266 0.0409 0.0181 0.0362 0.0375 0.0449 0.0491
Lipid Particles 0.1408 0.1293 0.13 0.1012 0.0756 0.1116 0.2883 0.1382 0.1694 0.0956 0.128 0.1921 0.2549 0.2994 0.2181 0.0658 0.1063 0.1663
Mitochondria 0.016 0.0025 0.0205 0.0109 0.0277 0.0112 0.0036 0.0076 0.0029 0.0044 0.0034 0.0015 0.0093 0.0004 0.01 0.0055 0.0022 0.0035
None 0.072 0.0262 0.0618 0.0301 0.0508 0.0799 0.0635 0.0679 0.022 0.0693 0.0812 0.0491 0.0316 0.0246 0.0226 0.0062 0.0014 0.0157
Nuclear Periphery 0.0124 0.0138 0.016 0.0228 0.0088 0.0174 0.0068 0.0063 0.0078 0.0042 0.0049 0.0038 0.0099 0.0045 0.0067 0.0052 0.006 0.0087
Nucleolus 0.0094 0.002 0.0024 0.0146 0.0239 0.0018 0.0003 0.0004 0.0007 0.0001 0.0002 0.0001 0.0012 0.0001 0.0001 0.0015 0.0001 0.0001
Nucleus 0.0125 0.007 0.0067 0.0326 0.0082 0.0077 0.0007 0.0016 0.002 0.0006 0.0011 0.0006 0.0016 0.001 0.0008 0.0011 0.0009 0.0012
Peroxisomes 0.0132 0.0049 0.0103 0.0091 0.0064 0.003 0.0245 0.0009 0.0018 0.0023 0.0017 0.0021 0.0075 0.0017 0.0019 0.0036 0.0018 0.0017
Punctate Nuclear 0.0204 0.0166 0.0152 0.0154 0.0159 0.0116 0.0018 0.0027 0.0028 0.0008 0.0013 0.0008 0.0056 0.0018 0.0014 0.0031 0.0008 0.0016
Vacuole 0.02 0.0193 0.0157 0.0117 0.0118 0.0149 0.0191 0.0123 0.0167 0.0061 0.0057 0.0074 0.0082 0.0122 0.0079 0.0107 0.0062 0.0095
Vacuole Periphery 0.0025 0.0018 0.002 0.0034 0.004 0.0022 0.0084 0.0009 0.0011 0.0011 0.001 0.0009 0.0015 0.0005 0.001 0.0021 0.0011 0.0012

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 46.9778 40.971 28.8821 29.1835 47.5385 28.6291 39.3544 32.503 33.9326 40.083
Translational Efficiency 0.9509 1.0546 1.0964 1.3594 1.0561 1.3803 1.0478 0.9862 0.9898 0.9014

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description 3-ketosphinganine reductase; catalyzes the second step in phytosphingosine synthesis; essential for growth in the absence of exogenous dihydrosphingosine or phytosphingosine; localized to lipid droplets; member of short chain dehydrogenase/reductase protein family
Localization
Cell Percentages ER (82%)
Cell Cycle Regulation No
Subcompartmental Group ER-3

Tsc10

Tsc10


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tsc10-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available