Standard name
Human Ortholog
Description Late endosomal protein involved in late endosome to vacuole transport; functional homolog of human obesity receptor gene-related protein (OB-RGRP)
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.14 0.14 0.22 0.09 0.17 0.11 – 0.07 0.06 0.05 0.06 0 0.18 0.16 0.12 0.12 0.1 0.09 0 0 0 0 0 0
Bud 0 0 0.07 0.06 0 0 – 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.75 0.82 0.64 0.64 0.56 0.52 – 0.27 0.43 0.35 0.38 0.37 0.78 0.7 0.78 0.82 0.83 0.76 0.83 0.86 0.85 0.82 0.84 0.85
Cytoplasm 0.26 0.21 0.3 0.23 0.31 0.38 – 0.49 0.4 0.5 0.35 0.37 0.38 0.37 0.38 0.18 0.17 0.27 0.05 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.06 0.06 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.3 0.18 0.4 0.71 0.47 0.57 – 0.52 0.64 0.56 0.66 0.7 0.18 0.24 0.17 0.09 0.1 0.11 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0.07 0.09 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 64 36 56 23 49 50 – 39 29 20 25 19 40 33 29 25 15 17 7 5 5 2 3 3
Bud 10 6 18 15 10 10 – 21 23 15 12 15 2 4 2 2 2 0 2 1 1 0 1 1
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 1 0 0 0 11 5 10 3 8 3
Bud Site 1 0 0 1 2 3 – 1 0 1 0 1 1 1 0 0 0 0 – – – – – –
Cell Periphery 341 214 162 159 161 229 – 141 208 145 146 181 169 144 186 166 131 146 427 254 248 81 170 158
Cytoplasm 117 55 75 57 91 168 – 258 196 208 135 180 82 75 91 37 27 51 25 8 6 2 4 3
Endoplasmic Reticulum 2 0 1 1 5 6 – 4 0 6 3 5 0 2 1 13 10 7 3 4 2 1 4 4
Endosome 2 0 0 0 0 0 – 2 0 0 0 0 0 0 2 0 0 0 8 1 2 0 0 1
Golgi 0 0 0 0 1 0 – 0 0 0 0 0 1 0 0 0 1 4 2 2 0 0 0 1
Mitochondria 134 48 102 175 136 252 – 278 308 233 256 337 39 49 40 19 16 22 7 5 6 0 1 3
Nucleus 2 2 0 0 1 0 – 4 0 2 2 4 0 1 0 3 4 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 4 0 0 1 3 0 0 0 0 1 0 1 0 0 0 0 0
Nucleolus 0 0 0 0 0 1 – 0 2 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0
Peroxisomes 1 0 0 0 2 4 – 1 2 2 0 0 7 15 21 2 0 1 0 0 0 1 0 0
SpindlePole 6 3 2 1 4 2 – 0 1 2 0 1 2 2 2 1 1 2 0 0 0 0 0 0
Vac/Vac Membrane 10 0 5 4 3 1 – 11 8 2 6 9 0 2 1 7 1 4 10 4 2 1 3 0
Unique Cell Count 453 260 254 248 290 444 530 484 418 385 484 217 205 239 202 157 192 513 297 291 100 203 187
Labelled Cell Count 690 364 421 436 465 726 764 777 636 586 755 344 328 377 275 209 254 513 297 291 100 203 187


Endosome

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.9 7.6 5.1 5.6 6.2 5.2 – 5.2 4.8 5.0 4.7 4.7 7.3 7.6 7.5 9.6 9.5 9.2 7.2 8.0 8.0
Std Deviation (1e-4) 0.7 1.0 1.2 1.3 1.5 1.0 – 1.0 1.1 0.9 1.0 1.0 1.5 1.7 1.7 1.7 1.7 2.1 1.3 1.8 1.8
Intensity Change (Log2) – – – 0.12 0.29 0.02 – 0.02 -0.09 -0.05 -0.11 -0.13 0.51 0.57 0.56 0.9 0.9 0.85 0.49 0.65 0.65


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -3.9 -1.5 -3.8 – -5.9 0 -6.9 0 0 -1.0 -1.6 -2.9 -2.7 -3.3 -3.7
Bud -0.5 -1.9 -3.1 – -1.9 0 -2.0 0 0 -3.3 -2.6 -3.5 -3.2 -2.7 -3.8
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.1 -2.0 -3.1 – -10.0 0 -7.3 0 0 3.3 1.5 3.4 4.3 4.3 2.8
Cytoplasm -1.7 0.5 2.2 – 5.1 2.9 5.2 1.5 2.1 1.9 1.6 2.0 -2.8 -2.8 -0.7
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 3.7 0 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 6.8 1.6 4.2 – 3.2 0 3.9 0 0 -5.2 -3.7 -5.7 -7.4 -6.5 -6.7
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 4.4 4.8 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 – 0.1 0 0 0 0 0 0 0 1.0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.7252 3.0299 2.4017 2.8676 1.5294 2.839 2.5367 2.5002 2.3628 2.0076 2.6321 2.8081 3.5985 3.8848 3.6899 4.6547 3.2532 3.8413
Actin 0.0101 0.0001 0.0144 0.0225 0 0.0152 0.0126 0.0005 0.0038 0.0088 0.0008 0.0016 0.0123 0.0001 0.0039 0.0164 0.0001 0.0001
Bud 0.0001 0.0001 0.0003 0.0004 0 0.0001 0.0006 0.0001 0.0007 0.0001 0.0001 0.0004 0.0002 0.0002 0.0003 0.0007 0 0
Bud Neck 0.0004 0.0011 0.0008 0.0012 0.0001 0.0029 0.0012 0.0011 0.0005 0.0002 0.0002 0.0006 0.0004 0.0004 0.0004 0.0002 0.0001 0.0004
Bud Periphery 0.0003 0.0001 0.0003 0.0004 0 0.0002 0.001 0.0001 0.0006 0.0001 0.0001 0.0003 0.0002 0.0003 0.0003 0.001 0 0
Bud Site 0.0008 0.0011 0.0024 0.0043 0 0.0009 0.0028 0.0011 0.0024 0.0004 0.0002 0.0006 0.0013 0.0021 0.001 0.0009 0.0001 0.0001
Cell Periphery 0.0003 0.0002 0.0001 0.0001 0 0.0001 0.0011 0.0001 0.0001 0 0 0.0001 0.0001 0.0001 0.0001 0.0001 0 0
Cytoplasm 0.0034 0.0113 0.0098 0.0009 0.0003 0.0388 0.0082 0.0047 0.0059 0.024 0.0013 0.0057 0.0015 0.0141 0.0034 0.0017 0.0006 0.016
Cytoplasmic Foci 0.1285 0.1431 0.1075 0.0809 0.0932 0.0918 0.1418 0.2032 0.1841 0.1283 0.1245 0.1743 0.1562 0.1971 0.2062 0.1195 0.1543 0.1702
Eisosomes 0.0007 0 0.0001 0.0002 0 0.0001 0.0008 0 0 0 0 0 0.0001 0 0.0001 0.0001 0 0
Endoplasmic Reticulum 0.001 0.0008 0.0032 0.0005 0.0002 0.0008 0.0056 0.0005 0.0015 0.0005 0.0012 0.0013 0.0009 0.0021 0.0006 0.0002 0.0002 0.0013
Endosome 0.4842 0.5229 0.5334 0.5819 0.6265 0.3877 0.5484 0.5387 0.5147 0.6347 0.5768 0.4518 0.4458 0.486 0.4982 0.6671 0.5696 0.4568
Golgi 0.1171 0.1125 0.1484 0.2005 0.0907 0.1099 0.0711 0.0712 0.1284 0.0804 0.0921 0.1094 0.0919 0.0874 0.1206 0.0709 0.106 0.0983
Lipid Particles 0.1602 0.1236 0.0889 0.027 0.1366 0.2961 0.0772 0.0765 0.064 0.0537 0.016 0.1497 0.1664 0.0992 0.0693 0.0669 0.1462 0.1662
Mitochondria 0.0123 0.0023 0.0074 0.0307 0.0013 0.0048 0.0215 0.0089 0.0061 0.0068 0.1211 0.0111 0.0219 0.002 0.0221 0.0116 0.0012 0.0016
None 0.0061 0.0012 0.0053 0.0001 0 0.0007 0.0107 0.0006 0.0025 0.001 0.0001 0.0026 0.0007 0.0151 0.0014 0.0002 0 0.0026
Nuclear Periphery 0.0015 0.0007 0.0031 0.0004 0.0002 0.0014 0.0111 0.0007 0.0015 0.0003 0.0008 0.0057 0.0024 0.0022 0.0003 0.0003 0.0002 0.0004
Nucleolus 0.0014 0.0064 0.0008 0.0001 0.0001 0.0003 0.0032 0.0007 0.0006 0.0003 0.0001 0.0106 0.0003 0.0029 0.0003 0.0001 0.0005 0.0004
Nucleus 0.0008 0.0011 0.0011 0.0001 0 0.0005 0.0021 0.0005 0.0003 0.0002 0.0001 0.003 0.0007 0.0006 0.0003 0.0001 0.0001 0.0001
Peroxisomes 0.021 0.0141 0.0262 0.0245 0.0222 0.0168 0.0346 0.0468 0.0638 0.0244 0.0566 0.0479 0.0717 0.0507 0.0497 0.0254 0.0144 0.0693
Punctate Nuclear 0.01 0.0008 0.0033 0.0003 0.0003 0.0013 0.0082 0.0059 0.0025 0.001 0.0003 0.0035 0.0037 0.0052 0.0012 0.0006 0.0006 0.0018
Vacuole 0.0296 0.0424 0.0283 0.0104 0.0179 0.011 0.0266 0.0311 0.011 0.0177 0.0044 0.0114 0.0156 0.025 0.0168 0.0104 0.0031 0.0103
Vacuole Periphery 0.0102 0.0141 0.0148 0.0126 0.0102 0.0185 0.0097 0.007 0.005 0.0171 0.003 0.0082 0.0059 0.007 0.0034 0.0057 0.0028 0.0039

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 36.5193 34.9779 29.4158 63.5096 27.0565 31.2849 45.3865 65.2439 45.686 52.1444
Translational Efficiency 1.8864 1.3723 1.8433 0.6249 1.7794 3.1288 1.4913 1.0782 1.6771 1.471

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Late endosomal protein involved in late endosome to vacuole transport; functional homolog of human obesity receptor gene-related protein (OB-RGRP)
Localization
Cell Percentages vacuole (20%), Golgi (8%), mixed (24%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Vps55

Vps55


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Vps55-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available