Standard name
Human Ortholog
Description Serine/threonine kinase and DNA damage checkpoint effector; mediates cell cycle arrest via phosphorylation of Pds1p; phosphorylated by checkpoint signal transducer Mec1p; homolog of S. pombe and mammalian Chk1 checkpoint kinase

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.92 0.89 0.87 0.92 0.94 0.82 0.85 0.83 0.69 0.75 0.65 0.69 0.93 0.97 0.94 0.73 0.6 0.71 0.77 0.65 0.89 0.9 0.81
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.11 0.14 0.17 0.17 0.15 0.31 0.28 0.32 0.48 0.44 0.49 0.54 0.06 0 0 0.06 0 0.1 0.07 0.15 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.15 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0.19 0.24 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 2 1 2 1
Bud 0 0 0 1 2 2 5 1 6 8 2 4 0 0 0 0 0 1 2 0 0 0 4
Bud Neck 0 0 0 1 0 2 1 6 5 3 2 0 0 0 0 0 0 0 1 0 0 0 0
Bud Site 1 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 1 1 0 0 2 0 1 0 2 2 0 0 0 0 0 0
Cytoplasm 120 155 86 101 116 157 195 278 137 182 82 106 66 176 153 176 99 135 142 63 84 150 119
Endoplasmic Reticulum 0 1 2 0 0 0 0 0 1 0 0 0 0 1 0 13 6 7 0 0 0 0 0
Endosome 2 0 0 0 0 0 0 0 0 0 0 0 0 0 3 9 5 5 2 2 0 0 1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 2 0 1 0
Mitochondria 14 24 17 19 19 60 64 109 96 107 62 83 4 1 1 14 7 19 13 14 2 4 6
Nucleus 0 2 0 1 1 0 1 2 0 1 0 1 0 0 1 15 24 1 6 2 0 0 0
Nuclear Periphery 0 2 0 0 0 0 0 1 1 1 0 0 0 0 0 1 0 0 0 0 0 1 0
Nucleolus 0 1 0 0 1 0 0 1 0 1 0 0 1 0 1 1 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 1 0 0 0 0
SpindlePole 0 0 1 0 1 3 5 5 2 9 2 7 1 0 0 2 1 3 4 2 0 0 1
Vac/Vac Membrane 2 3 5 1 0 1 0 3 1 3 0 0 2 4 6 45 40 5 5 4 0 2 5
Unique Cell Count 131 174 99 110 124 192 230 336 200 244 126 154 71 182 162 241 164 192 186 99 95 167 147
Labelled Cell Count 139 188 111 124 140 225 271 407 250 315 150 205 75 183 165 278 184 192 186 99 95 167 147


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.2 3.2 3.7 3.0 3.2 2.9 2.5 3.0 2.7 2.5 2.7 2.4 4.3 4.2 4.3 4.9 5.5 5.8 3.9 4.1 5.0
Std Deviation (1e-4) 0.9 0.7 1.9 1.0 1.4 1.3 1.4 1.6 1.5 1.6 1.7 1.1 1.9 1.6 1.9 1.5 1.7 1.1 1.2 1.6 2.2
Intensity Change (Log2) -0.3 -0.21 -0.36 -0.57 -0.29 -0.48 -0.56 -0.46 -0.66 0.21 0.17 0.21 0.38 0.57 0.64 0.06 0.12 0.42


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0
Bud Neck 0 0
Bud Site 0 0
Cell Periphery 0 0
Cyto
Endoplasmic Reticulum 0 0
Endosome 0 0
Golgi 0 0
Mitochondria 0 0
Nuclear Periphery 0 0
Nuc
Nucleolus 0 0
Peroxisomes 0 0
SpindlePole 0 0
Vac
Cortical Patches 0 0
Cytoplasm 0 0
Nucleus 0 0
Vacuole 3.2 4.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.0218 0.9252 0.6434 0.3191 0.2486 0.4383 0.0788 -0.2734 0.2784 0.1009 -0.17 0.1905 -0.1579 0.6076 0.2294 0.4846 -0.1494 0.0178
Actin 0 0.0259 0.0021 0.0003 0.0001 0.0012 0.0401 0.0003 0.0256 0.0124 0.0003 0.0032 0.0008 0.0004 0.001 0.0001 0.0004 0.0001
Bud 0 0.0003 0.0001 0.0001 0 0.0002 0.0011 0.0004 0.0002 0.0016 0.0008 0.0001 0 0.0001 0.0001 0 0 0
Bud Neck 0 0.0009 0.0002 0.0001 0.0001 0.002 0.0147 0 0.0005 0.0002 0.0004 0.0002 0.0001 0 0.0001 0 0.0002 0.0001
Bud Periphery 0 0.0003 0.0002 0.0003 0 0.0007 0.0018 0.0005 0.0003 0.0028 0.0009 0.0001 0 0.0001 0.0001 0 0 0
Bud Site 0 0.018 0.0007 0.0001 0 0.0006 0.036 0.0004 0.0027 0.0005 0.0016 0.0001 0.0002 0.0002 0.0004 0 0.0001 0
Cell Periphery 0 0.0005 0.0002 0.0002 0 0.0001 0.0011 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0 0.0001 0
Cytoplasm 0.0326 0.0431 0.0671 0.0282 0.0669 0.0487 0.0263 0.0399 0.0178 0.0076 0.0586 0.0266 0.0575 0.0558 0.0533 0.047 0.171 0.0967
Cytoplasmic Foci 0.0004 0.0022 0.0116 0.0286 0.0011 0.0173 0.0277 0.0046 0.0112 0.0053 0.005 0.0269 0.007 0.0034 0.0061 0.0016 0.0019 0.0022
Eisosomes 0.0001 0.0011 0.0004 0.0008 0.0001 0.0002 0.0017 0.0003 0.0008 0.0007 0.0002 0.0002 0.0003 0.0003 0.0002 0.0002 0.0002 0
Endoplasmic Reticulum 0.0003 0.0009 0.001 0.0016 0.0006 0.0006 0.0035 0.0008 0.0006 0.0007 0.0011 0.0003 0.002 0.0042 0.0008 0.0004 0.0034 0.0005
Endosome 0 0.0006 0.0056 0.0167 0.0004 0.0004 0.0271 0.0047 0.0112 0.0146 0.0142 0.0084 0.0132 0.026 0.0019 0.0002 0.0019 0.0007
Golgi 0 0.0007 0.0013 0.0013 0 0.0001 0.0101 0.0006 0.0098 0.0403 0.0017 0.0082 0.0004 0.0026 0.0024 0 0.0001 0
Lipid Particles 0 0.0004 0.0164 0.0042 0.0001 0.0005 0.0178 0.0004 0.0059 0.0024 0.0015 0.0162 0.0009 0.0005 0.0066 0.0001 0.0001 0
Mitochondria 0 0.0015 0.0027 0.0024 0.0001 0.0002 0.0521 0.0074 0.0278 0.0576 0.0574 0.0182 0.0002 0.0015 0.0051 0.0001 0.0003 0.0001
None 0.9663 0.9018 0.8557 0.8247 0.8559 0.9227 0.6926 0.9361 0.8601 0.8434 0.8121 0.8733 0.8942 0.901 0.8938 0.9489 0.7345 0.8959
Nuclear Periphery 0 0.0003 0.0029 0.0016 0.0014 0.0001 0.004 0.0003 0.0003 0.001 0.0014 0.0001 0.0057 0.0018 0.0006 0.0002 0.0119 0.0002
Nucleolus 0 0.0001 0.0006 0.0006 0.0005 0.0003 0.0037 0.0001 0.0003 0.0006 0.0006 0.0002 0.0004 0.0001 0.0008 0.0001 0.0005 0.0001
Nucleus 0.0001 0.0004 0.0054 0.0827 0.0691 0.0004 0.0025 0.0004 0.0191 0.0016 0.0211 0.0001 0.0046 0.0003 0.0161 0.0004 0.0648 0.0014
Peroxisomes 0 0.0004 0.0138 0.002 0 0.0004 0.0239 0.0006 0.0038 0.0023 0.0031 0.0158 0.0001 0.0001 0.0051 0 0 0.0001
Punctate Nuclear 0 0.0006 0.011 0.0014 0.0032 0.0032 0.0087 0.0002 0.0004 0.0025 0.002 0.0009 0.0073 0.0002 0.0052 0.0004 0.008 0.0017
Vacuole 0 0.0002 0.0008 0.0015 0.0003 0.0002 0.0027 0.0012 0.0009 0.0008 0.0141 0.0005 0.0048 0.0009 0.0004 0.0001 0.0004 0.0002
Vacuole Periphery 0 0 0.0002 0.0005 0 0 0.001 0.0005 0.0006 0.0011 0.0019 0.0003 0.0003 0.0007 0.0001 0 0.0001 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 12.1904 15.5678 9.7037 15.896 13.999 9.7469 13.0647 12.1112 15.9712 17.0358
Translational Efficiency 0.6642 0.5483 0.8527 0.5178 0.5348 0.8818 0.718 0.798 0.5622 0.4216

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1175 1092 265 1524 105 1795 422 19 1280 2887 687 1543

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 585.26 678.02 809.58 781.91 685.45 652.75 851.46 881.43 593.48 662.31 835.31 783.14
Standard Deviation 63.82 92.63 90.52 98.71 63.13 90.72 86.78 171.83 69.44 92.26 90.57 100.54
Intensity Change Log 2 0.212250 0.468096 0.417925 -0.070521 0.312887 0.362795 0.066626 0.386452 0.388448

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000024 0.000419 0.000427 0.000577 0.000065 0.000291 0.000122 0.000560 0.000028 0.000339 0.000240 0.000577
Bud Neck 0.003064 0.039449 0.003586 0.010330 0.005695 0.040274 0.002465 0.002110 0.003279 0.039962 0.002898 0.010229
Bud Site 0.001041 0.013709 0.004195 0.030697 0.000949 0.010597 0.005675 0.003262 0.001033 0.011774 0.005104 0.030359
Cell Periphery 0.000156 0.000160 0.000172 0.000151 0.000321 0.000132 0.000117 0.000370 0.000170 0.000143 0.000138 0.000154
Cytoplasm 0.447407* 0.370434* 0.170220* 0.422610* 0.371304* 0.404844* 0.348499* 0.366272* 0.441164* 0.391828* 0.279730* 0.421916*
Cytoplasmic Foci 0.072001 0.273252* 0.011036 0.008704 0.321241* 0.255838* 0.018586 0.092379 0.092447 0.262425* 0.015674 0.009734
Eisosomes 0.000084 0.000174 0.000075 0.000031 0.000332 0.000163 0.000058 0.000245 0.000105 0.000167 0.000064 0.000034
Endoplasmic Reticulum 0.000137 0.000668 0.006473 0.002240 0.000437 0.000518 0.001737 0.001506 0.000162 0.000575 0.003564 0.002231
Endosome 0.001924 0.027015 0.002598 0.001674 0.017690 0.020510 0.002686 0.033960 0.003218 0.022971 0.002652 0.002071
Golgi 0.000159 0.007994 0.000097 0.000454 0.004576 0.003616 0.000048 0.011145 0.000521 0.005272 0.000067 0.000586
Lipid Particles 0.002889 0.009699 0.002775 0.000825 0.027106 0.011030 0.001692 0.004863 0.004875 0.010527 0.002109 0.000874
Mitochondria 0.001128 0.019483 0.000513 0.001516 0.007129 0.012585 0.000272 0.000209 0.001620 0.015195 0.000365 0.001499
Mitotic Spindle 0.001624 0.005028 0.003046 0.017373 0.000472 0.005026 0.002688 0.000791 0.001529 0.005027 0.002826 0.017169
None 0.009392 0.004100 0.003888 0.006766 0.002671 0.004163 0.004718 0.001890 0.008840 0.004139 0.004398 0.006706
Nuclear Periphery 0.000421 0.000443 0.001633 0.001711 0.000863 0.000633 0.001672 0.000976 0.000457 0.000561 0.001657 0.001702
Nuclear Periphery Foci 0.000091 0.000344 0.001365 0.000756 0.000459 0.000353 0.000763 0.000744 0.000121 0.000350 0.000995 0.000756
Nucleolus 0.001328 0.002853 0.001053 0.000383 0.002281 0.002373 0.000596 0.000576 0.001406 0.002554 0.000772 0.000386
Nucleus 0.450755* 0.123272 0.743377* 0.437345* 0.193825* 0.146074* 0.527328* 0.351304* 0.429678* 0.137450* 0.610666* 0.436285*
Peroxisomes 0.000873 0.024536 0.000514 0.002080 0.011861 0.018538 0.000187 0.001234 0.001774 0.020807 0.000313 0.002070
Vacuole 0.005343 0.073676 0.042734 0.053477 0.029554 0.060718 0.079975 0.124102 0.007329 0.065619 0.065610 0.054347
Vacuole Periphery 0.000160 0.003289 0.000224 0.000302 0.001170 0.001722 0.000117 0.001501 0.000243 0.002315 0.000158 0.000317

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -8.11 -9.68 -7.38 -0.34 1.12 -5.13 -3.87 -1.13 -0.48 -0.76 -10.08 -10.90 -7.39 -1.78 -2.04
Bud Neck -12.32 -0.56 -6.13 9.72 -4.74 -13.79 1.87 2.72 17.16 1.58 -20.28 -0.07 -5.99 15.43 -6.07
Bud Site -10.69 -1.97 -7.41 -1.42 -4.90 -9.51 -1.96 -1.29 4.09 0.66 -13.39 -2.59 -7.43 -2.32 -5.06
Cell Periphery -0.08 -2.39 1.98 1.36 3.78 5.41 6.04 -0.18 -3.09 -3.40 2.56 2.39 3.06 0.29 0.62
Cytoplasm 7.04 19.03 13.71 5.45 -10.25 -1.52 3.15 0.38 0.94 -0.80 5.67 17.30 13.39 9.61 -6.44
Cytoplasmic Foci -26.75 19.34 22.08 38.21 2.69 3.39 15.62 8.84 8.64 -2.96 -30.24 20.72 22.83 59.35 4.08
Eisosomes -7.00 -1.17 7.43 10.44 6.27 4.89 7.45 1.48 -1.02 -2.41 -7.81 3.73 9.48 19.72 6.99
Endoplasmic Reticulum -9.71 -10.37 -20.18 -16.75 5.48 -0.72 -9.76 -2.40 -2.29 1.47 -9.54 -13.82 -20.14 -17.61 3.02
Endosome -13.67 -1.88 0.62 14.82 5.15 -0.70 3.99 -0.39 -0.25 -1.20 -16.19 0.27 1.94 20.26 3.68
Golgi -8.29 1.40 -0.48 8.19 -1.01 0.37 1.86 -0.42 -0.55 -1.01 -9.99 2.17 0.85 10.96 -1.61
Lipid Particles -6.28 -0.89 4.64 8.52 6.50 2.14 3.40 2.98 5.30 -3.30 -5.92 3.32 5.14 15.01 7.26
Mitochondria -7.85 1.60 -0.04 7.93 -3.89 -1.51 1.87 1.92 11.35 3.87 -11.39 2.65 1.03 12.64 -5.12
Mitotic Spindle -2.39 -0.37 -5.60 -3.82 -4.07 -3.97 -2.16 -0.59 3.36 1.97 -2.94 -1.62 -5.74 -4.02 -3.18
None 3.95 4.58 2.99 -2.45 -4.34 -1.46 -1.61 0.97 2.97 3.47 3.96 4.29 2.80 -3.01 -4.44
Nuclear Periphery -0.56 -13.34 -22.43 -22.70 -5.74 0.65 -5.07 -0.94 -2.09 4.59 -1.31 -18.18 -21.87 -18.64 -4.64
Nuclear Periphery Foci -7.15 -7.46 -5.71 -4.22 2.47 0.87 -3.79 -1.53 -2.23 1.35 -3.95 -10.78 -5.58 -4.12 1.05
Nucleolus -3.34 1.25 4.03 5.46 5.63 -0.23 2.99 3.20 7.19 0.99 -4.02 3.05 4.64 8.99 3.97
Nucleus 34.43* -15.75 -5.09 -42.67 12.82 2.25 -13.72 -2.47 -3.34 2.78 34.66 -13.26 -6.98 -45.88 7.97
Peroxisomes -12.72 1.71 -0.11 12.61 -1.32 -1.45 2.68 2.48 11.81 -1.95 -15.85 3.61 1.73 17.04 -2.08
Vacuole -20.12 -9.69 -23.24 -6.80 -6.48 -8.97 -10.25 -3.13 -2.45 -0.89 -30.79 -15.31 -23.06 -9.96 -1.52
Vacuole Periphery -6.82 -2.12 -0.45 6.77 1.46 -1.25 2.54 0.06 0.76 -1.27 -10.15 1.59 0.90 10.48 -0.57