Standard name
Human Ortholog
Description Third-largest subunit of DNA polymerase II (DNA polymerase epsilon); required to maintain fidelity of chromosomal replication and also for inheritance of telomeric silencing; stabilizes the interaction of Pol epsilon with primer-template DNA, positively affecting the processivity of the polymerase and exonuclease activities of Pol epsilon; mRNA abundance peaks at the G1/S boundary of the cell cycle; DPB3 has a paralog, DLS1, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.05 0.06 0.08 0.06 0.05 0.05 0.05 0 0.1 0.18 0.16 0.05 0.09 0.07 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.06 0 0 0 0.35 0.12 0.29 0.27 0.33 0.36 0 0 0 0 0 0.05 0 0 0 0 0 0
Nucleus 0.95 0.91 0.93 0.91 0.86 0.79 0.88 0.79 0.82 0.78 0.79 0.88 0.79 0.79 0.86 0.85 0.89 0.86 0.89 0.85 0.77 0.64 0.55
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0.05 0.06 0.05 0.09 0.16 0.21 0.23
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Bud 0 0 0 0 0 2 3 6 3 9 3 0 0 0 0 1 0 2 0 1 1 5 6
Bud Neck 0 0 0 0 0 0 0 1 1 3 1 0 0 0 0 0 0 0 0 0 0 2 3
Bud Site 0 0 0 2 1 11 1 7 13 16 26 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3 5 13 16 22 33 29 26 27 30 13 25 68 58 14 21 13 1 0 3 3 4 3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 5 7 4 0 0 0 0 0 1
Endosome 1 0 1 4 9 2 3 1 1 3 3 0 3 3 4 3 0 5 2 0 1 6 9
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 1 1
Mitochondria 7 9 4 7 10 135 59 152 142 197 203 5 15 2 9 4 9 1 1 1 0 9 11
Nucleus 269 142 305 293 304 307 437 408 423 468 441 220 301 292 222 206 173 256 144 298 141 229 165
Nuclear Periphery 1 0 0 0 2 2 7 5 5 5 4 0 1 1 1 0 3 0 0 0 0 0 2
Nucleolus 3 1 1 0 2 5 8 15 11 12 13 2 4 10 4 3 1 7 0 6 1 12 13
Peroxisomes 1 0 0 1 0 0 1 0 0 0 0 0 0 4 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 4 6 5 4 12 3 9 3 7 0 4 4 3 1 0 2 0 3 1 5 7
Vac/Vac Membrane 6 10 8 7 13 9 11 20 9 17 21 7 8 9 15 13 10 16 8 30 28 73 69
Unique Cell Count 284 156 327 322 352 389 498 517 519 599 559 249 382 368 257 243 195 298 162 350 184 356 303
Labelled Cell Count 292 167 336 336 368 510 571 644 644 763 735 259 404 383 277 259 214 298 162 350 184 356 303


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.4 5.2 6.0 5.7 6.4 4.3 5.2 4.7 4.9 4.7 4.6 6.4 5.5 6.7 7.0 7.9 6.8 5.3 5.7 5.9
Std Deviation (1e-4) 0.8 0.7 1.2 1.4 3.7 1.2 1.4 1.2 1.1 1.0 0.9 1.6 1.6 2.0 1.4 1.9 1.4 1.5 1.7 1.6
Intensity Change (Log2) -0.07 0.09 -0.48 -0.2 -0.36 -0.3 -0.36 -0.39 0.09 -0.12 0.16 0.23 0.41 0.17 -0.17 -0.07 -0.03


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.6 1.3 2.5 1.2 0.7 0.8 0.7 -1.4 2.9 5.8 5.1 0.8 2.3 1.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.9 1.5 0 5.6 0 0 0 0 0 2.2 0 1.9 0 0
Nucleus -1.1 -3.0 -5.4 -2.6 -5.6 -4.8 -5.9 -5.7 -2.1 -5.5 -5.3 -2.8 -3.3 -1.8
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 2.5 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 1.2 0 0 0 0 0 0 0 0 0 0
Vacuole -0.2 0.9 -0.1 -0.2 1.1 -0.7 0.4 1.1 0.3 -0.3 0 2.1 1.8 1.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.6374 0.3493 0.3408 0.3504 0.7979 0.2912 3.5936 3.8457 3.74 3.981 3.6865 3.6867 3.5714 4.8955 4.4745 4.1044 4.4675 4.5753
Actin 0.051 0.0001 0.0033 0.0005 0.0028 0.0087 0.0192 0.0002 0.0083 0.0053 0.0157 0.0093 0.0224 0 0.0001 0 0 0.0058
Bud 0.0011 0 0.0001 0.0002 0.0001 0.0007 0.0004 0 0 0.0001 0.0002 0.0001 0.0001 0 0 0 0 0
Bud Neck 0.0034 0.0002 0.0004 0.0007 0.0009 0.0022 0.0045 0.0006 0.0003 0.0055 0.0007 0.0079 0.0009 0.0003 0.0001 0 0.0004 0.0011
Bud Periphery 0.0012 0 0.0001 0.0001 0.0001 0.002 0.0007 0 0.0001 0.0002 0.0004 0.0001 0.0002 0 0 0 0 0
Bud Site 0.013 0.0001 0.0006 0.0039 0.0005 0.0014 0.0018 0.0002 0.0002 0.0002 0.0006 0.0003 0.0005 0.0003 0.0002 0 0 0.0001
Cell Periphery 0.0004 0 0 0 0 0.0002 0.0002 0 0 0 0.0001 0 0 0 0 0 0 0
Cytoplasm 0.0163 0.0118 0.014 0.0009 0.0011 0.0042 0.014 0.0143 0.0068 0.0063 0.0115 0.0029 0.0045 0.0134 0.0087 0.0001 0.0005 0.0042
Cytoplasmic Foci 0.0139 0.0001 0.0012 0.028 0.0198 0.0032 0.0032 0.0002 0.0021 0.0008 0.0042 0.0016 0.0014 0.0001 0.0002 0 0 0.0006
Eisosomes 0.0003 0 0 0 0 0.0001 0.0003 0 0.0001 0.0001 0.0002 0 0.0002 0 0 0 0 0.0001
Endoplasmic Reticulum 0.0016 0.0004 0.0035 0 0.0013 0.0008 0.0042 0.0005 0.0008 0.0006 0.0031 0.0002 0.0007 0.0001 0.0013 0 0 0.0003
Endosome 0.0177 0.0003 0.0054 0.003 0.0319 0.0051 0.009 0.0005 0.0021 0.0023 0.0112 0.0015 0.0024 0.0001 0.0003 0 0 0.001
Golgi 0.0078 0 0.0005 0.0027 0.014 0.0031 0.0015 0 0.0017 0.0012 0.0031 0.0014 0.001 0 0 0 0 0.0005
Lipid Particles 0.0124 0 0.0013 0.0036 0.0809 0.0031 0.0035 0 0.0025 0.0027 0.006 0.0032 0.0019 0 0.0001 0 0 0.0017
Mitochondria 0.0031 0.0001 0.0012 0.0005 0.0028 0.0066 0.0014 0.0003 0.0006 0.0006 0.0043 0.0005 0.0017 0.0001 0.0002 0 0.0001 0.0002
None 0.0084 0.0014 0.0029 0.0003 0.0004 0.0193 0.0302 0.0179 0.0039 0.0004 0.007 0.0051 0.0012 0.0095 0.0237 0 0.0001 0.0079
Nuclear Periphery 0.0281 0.0096 0.0187 0.0054 0.0784 0.0047 0.0252 0.0076 0.0033 0.0149 0.0265 0.0037 0.0051 0.0044 0.0076 0.0012 0.002 0.0028
Nucleolus 0.0059 0.0057 0.0262 0.0048 0.0037 0.0191 0.0078 0.0065 0.0121 0.0078 0.01 0.0184 0.0063 0.0072 0.0109 0.0021 0.0194 0.016
Nucleus 0.7919 0.9588 0.8963 0.932 0.7425 0.8968 0.851 0.9397 0.9336 0.9365 0.8669 0.9156 0.9329 0.9558 0.9407 0.9964 0.965 0.9388
Peroxisomes 0.0084 0 0.0001 0.0081 0.0099 0.009 0.0029 0 0.0014 0.0008 0.0015 0.0044 0.0018 0 0 0 0 0.0009
Punctate Nuclear 0.0103 0.011 0.0229 0.005 0.0064 0.0064 0.0158 0.0106 0.0198 0.013 0.025 0.0235 0.0143 0.0082 0.0055 0.0001 0.0125 0.0177
Vacuole 0.0026 0.0003 0.0007 0.0002 0.0012 0.0023 0.0025 0.0004 0.0002 0.0003 0.001 0.0002 0.0004 0.0004 0.0002 0 0 0.0001
Vacuole Periphery 0.0013 0.0001 0.0004 0.0001 0.0013 0.0008 0.001 0.0001 0.0001 0.0003 0.001 0.0001 0.0002 0.0001 0.0001 0 0 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 30.0432 17.9045 15.7015 16.1947 20.0663 30.9365 11.1088 13.8846 17.3944 22.071
Translational Efficiency 1.1426 0.9522 0.9185 1.1708 1.298 1.0715 1.4092 1.2631 1.0385 1.2037

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1910 1216 213 1937 1527 2324 1772 1787 3437 3540 1985 3724

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 691.19 808.93 946.27 896.91 703.10 809.79 873.85 978.22 696.48 809.49 881.62 935.93
Standard Deviation 307.63 113.39 101.26 118.77 84.41 99.98 121.31 129.07 236.20 104.78 121.41 130.31
Intensity Change Log 2 0.226933 0.453170 0.375881 0.203818 0.313656 0.476429 0.215323 0.384503 0.427460

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000152 0.000375 0.000751 0.000472 0.000120 0.000161 0.000424 0.000612 0.000138 0.000235 0.000459 0.000540
Bud Neck 0.003917 0.005682 0.004429 0.015596 0.001527 0.005872 0.007524 0.020735 0.002855 0.005807 0.007192 0.018062
Bud Site 0.001792 0.001559 0.000906 0.005106 0.000695 0.002132 0.000930 0.008431 0.001304 0.001935 0.000928 0.006702
Cell Periphery 0.000262 0.000182 0.000080 0.000141 0.000120 0.000132 0.000084 0.000104 0.000199 0.000149 0.000084 0.000123
Cytoplasm 0.038767 0.033204 0.002496 0.011126 0.023076 0.021036 0.007242 0.026250 0.031796 0.025216 0.006733 0.018384
Cytoplasmic Foci 0.006053 0.005083 0.000056 0.000114 0.001926 0.002411 0.000072 0.001746 0.004219 0.003329 0.000070 0.000897
Eisosomes 0.000021 0.000021 0.000028 0.000016 0.000013 0.000015 0.000016 0.000018 0.000018 0.000017 0.000017 0.000017
Endoplasmic Reticulum 0.001888 0.006168 0.007255 0.007059 0.001549 0.003338 0.004783 0.005271 0.001737 0.004310 0.005048 0.006201
Endosome 0.000690 0.001494 0.000276 0.000560 0.000257 0.001054 0.000196 0.001188 0.000498 0.001205 0.000205 0.000861
Golgi 0.000228 0.000397 0.000054 0.000129 0.000096 0.000735 0.000034 0.000825 0.000169 0.000619 0.000036 0.000463
Lipid Particles 0.001200 0.001128 0.000182 0.000105 0.000728 0.000724 0.000101 0.000222 0.000990 0.000863 0.000110 0.000161
Mitochondria 0.002864 0.001330 0.000690 0.001852 0.000805 0.004894 0.000711 0.003130 0.001949 0.003670 0.000709 0.002466
Mitotic Spindle 0.000810 0.004122 0.000189 0.010083 0.000829 0.004113 0.001140 0.015924 0.000819 0.004116 0.001038 0.012886
None 0.013219 0.011051 0.002837 0.004026 0.007527 0.006193 0.002749 0.006207 0.010690 0.007862 0.002759 0.005073
Nuclear Periphery 0.001078 0.001391 0.000481 0.002412 0.000573 0.001714 0.000638 0.001687 0.000854 0.001603 0.000621 0.002064
Nuclear Periphery Foci 0.000611 0.000853 0.000195 0.000232 0.000475 0.000544 0.000121 0.000341 0.000551 0.000650 0.000129 0.000284
Nucleolus 0.011627 0.011264 0.003076 0.002428 0.006391 0.007728 0.001848 0.002136 0.009301 0.008942 0.001980 0.002288
Nucleus 0.912088* 0.911275* 0.973525* 0.933023* 0.951757* 0.931869* 0.969816* 0.899310* 0.929712* 0.924795* 0.970214* 0.916846*
Peroxisomes 0.000920 0.000698 0.000165 0.000067 0.000395 0.000816 0.000063 0.000211 0.000687 0.000776 0.000074 0.000136
Vacuole 0.001449 0.002372 0.002255 0.004976 0.001032 0.003539 0.001445 0.005030 0.001264 0.003138 0.001532 0.005002
Vacuole Periphery 0.000366 0.000350 0.000072 0.000475 0.000110 0.000980 0.000062 0.000623 0.000252 0.000764 0.000063 0.000546

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.78 -4.89 -12.00 -5.27 2.00 -1.06 -8.80 -10.45 -11.69 -5.10 -3.48 -11.60 -15.54 -14.23 -4.24
Bud Neck -2.62 -2.81 -12.25 -10.45 -7.50 -7.25 -9.93 -15.50 -12.00 -8.46 -6.18 -8.83 -19.56 -15.71 -9.53
Bud Site 0.38 -0.43 -2.44 -2.78 -1.34 -2.53 -1.38 -5.41 -3.99 -5.13 -1.53 0.09 -5.66 -4.61 -5.92
Cell Periphery 1.51 5.17 4.99 1.72 -0.83 -0.51 1.61 0.96 2.21 -1.54 1.96 5.87 4.88 2.46 -1.93
Cytoplasm 1.48 15.36 12.26 7.92 -6.66 0.87 7.65 -0.16 -1.13 -8.81 3.18 15.43 8.47 5.13 -8.99
Cytoplasmic Foci 0.81 7.93 8.05 5.12 0.59 -0.67 5.13 2.19 2.71 -2.31 1.58 9.33 7.99 6.06 -2.31
Eisosomes -0.26 -3.20 -0.23 0.10 3.23 -1.06 -3.19 -8.24 -7.99 -6.92 0.43 -1.35 -5.22 -6.16 -4.24
Endoplasmic Reticulum -11.04 -8.55 -17.06 -3.18 0.83 -7.44 -17.77 -21.38 -10.45 -4.54 -12.18 -18.77 -25.74 -10.43 -5.70
Endosome -1.77 1.52 1.13 2.18 -0.55 -4.14 0.59 -3.37 0.49 -3.69 -3.41 3.32 -1.70 2.26 -4.47
Golgi -0.82 1.59 1.46 1.51 -0.22 -2.29 1.40 -1.71 0.88 -2.03 -2.25 2.76 -0.87 1.65 -2.31
Lipid Particles 0.43 4.63 5.19 5.07 1.31 -0.05 2.46 1.66 2.97 -4.04 0.75 5.52 4.87 5.62 -3.70
Mitochondria 2.13 2.91 1.06 -3.55 -7.63 -4.69 -0.04 -4.31 2.14 -4.85 -2.49 2.84 -1.14 2.00 -7.59
Mitotic Spindle -2.16 -0.30 -5.12 -2.64 -4.57 -3.12 -1.26 -6.78 -5.03 -6.14 -3.88 -1.44 -8.46 -5.52 -7.24
None 1.16 6.57 7.28 4.68 -0.97 1.08 5.10 1.71 0.60 -4.66 2.63 9.27 6.93 4.13 -4.48
Nuclear Periphery -2.07 4.00 -4.41 -3.41 -5.64 -5.12 -2.87 -13.85 -3.38 -13.01 -4.77 2.20 -8.90 -4.17 -9.65
Nuclear Periphery Foci -0.82 1.81 1.80 2.78 -0.27 -0.12 1.37 0.05 0.39 -5.01 -0.35 2.65 1.33 2.68 -4.57
Nucleolus 0.42 4.23 6.20 5.37 0.39 -0.91 4.03 2.64 4.18 -1.92 0.63 7.96 6.65 6.63 -1.97
Nucleus 0.12 -8.34 -3.06 -2.75 6.28 4.08 -3.46 10.88 7.13 15.09 1.13 -11.09 4.96 3.66 16.81
Peroxisomes 1.15 2.86 4.05 4.93 2.09 -1.70 2.47 1.76 3.02 -2.06 -0.19 4.53 4.39 4.46 -0.74
Vacuole -2.73 -3.18 -8.29 -6.80 -4.69 -4.01 -2.55 -9.42 -6.74 -8.19 -4.59 -2.93 -12.52 -8.89 -10.08
Vacuole Periphery 0.14 3.60 0.48 0.37 -2.21 -3.47 2.24 -2.51 2.20 -2.91 -2.98 4.21 -1.08 2.32 -3.77
Standard name
Human Ortholog
Description Third-largest subunit of DNA polymerase II (DNA polymerase epsilon); required to maintain fidelity of chromosomal replication and also for inheritance of telomeric silencing; stabilizes the interaction of Pol epsilon with primer-template DNA, positively affecting the processivity of the polymerase and exonuclease activities of Pol epsilon; mRNA abundance peaks at the G1/S boundary of the cell cycle; DPB3 has a paralog, DLS1, that arose from the whole genome duplication
Localization
Cell Percentages nucleus (96%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Dpb3

Dpb3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Dpb3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available